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Molecular Cloning, and Characterization of an Adenylyl Cyclase-Associated Protein from Gossypium arboreum L.

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Agricultural Sciences in China 2009年第7期8卷 777-783页

作者： WANG Sheng zhao Guo-hong JIA yin-hua du Xiong-ming Cotton Germplasm Division Cotton Research Institute Chinese Academy of Agricultural Sciences Anyang 455000 P.R.China

The aim of this study was to clone CAP （adenylyl cyclase-associated protein） gene from Gossypium arboreum L. and develop a platform for expressing and purifying CAP protein, which is a base for the construction and function researches of CAP. In this work, a CAP homolog from cotton （DPL971） ovule was identified and cloned. And the cDNA sequence consisted of an open reading frame of 1 416 nucleotides encoding a protein of 471 amino acid residues with a calculated molecular weight of 50.6 kDa. To gain insight on the CAP role in cotton fiber development, the cloned CAP cDNA was expressed. A significant higher yield pure protein was obtained with the chromatographic method. Further experiments showed that the purified protein can bind with the actin in vitro indicating that the recombinant cotton CAP is functional. The procedure described here produced high yield pure protein through one chromatographic step, suitable for further structure-function studies.

关键词： adenylyl cyclase-associated protein CAP cotton fiber protein expression protein purification Gossypiumarboreum L.

Identification and Characterization of Cotton Genes Involved in Fuzz-Fiber Development

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Journal of Integrative Plant Biology 2013年第7期55卷 619-630页

作者： Gaskin Wang Guo-Hong zhao yin-hua Jia Xiong-Ming du State Key Laboratory of Cotton Biology Institute of Cotton Research of the Chinese Academy of Agricultural Sciences

Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence in Arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of GLABRA2 （GL2） controlling trichome cell differentiation; it is also assumed that TRIPTYCHON （TRY） and CAPRICE （CPC） counteract the complex formation in neighboring cells. Here, the homologs GaMYB23 （a homolog of GL1）, GaDEL65 （a homolog of GL3）, GaTTG1, GaCPC and GaTRY were identified in Gossypium arboreum. We show that GaMYB23 can bind to and activate the promoters of GaCPC, GaGL2 and GaTRY, and that GaMYB23, GaTRY and GaTTG1 could interact with GaDEL65 in yeast and in planta. In situ analysis showed that GaMYB23, GaGL2, GaDEL65, and GaTRY were predominantly expressed in fuzz fiber, but GaTRY proteins were primarily found in undeveloped epidermal cells. A G. arboreum fuzzless mutant with consistently high level GaMYB23 transcript has lost the detectable GaMYB23-promoter of GaGL2 complex, corresponding to sharply reduced transcription of GaGL2. Our results support that cotton homologs to the genetic molecules regulating Arabidopsis trichome differentiation interacted in the epidermis of ovules and the redundant GaMYB23 serves as a negative regulator in fuzz-fiber patterning.

关键词： Convergent evolution fuzz, fuzzless mutant Gossypium arboreum lateral inhibitor trichome.

MOVPE growth of InAs quantum dots for mid-IR applications

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中国有色金属学会会刊：英文版 2006年第B01期16卷 25-28页

作者： TANG Xiao-hong yin Zong-you du An-yan zhao Jing-hua DENY S Photonics Research Center School of Electrical and Electronic Engineering Nanyang Technological University 639798 Singapore Institute of Microelectronics 11 Science Park Road 117685 Singapore

InAs quantum dots (QDs) grown on InxGa1-xAs/InP matrix by low pressure metal organic vapor phase epitaxy (LP-MOVPE) in nitrogen ambient were studied. Formation of the InAs QDs with different growth conditions was investigated. To improve the dot size uniformity, a two-step growth method was used and investigated. It is found that morphology of the InAs QDs formed on such InxGa1-xAs/InP matrix is very sensitive to the growth conditions. InAs QDs with high density of 1.3×1010 cm?2 are grown by using S-K growth method with fast growth rate. Using the two-step growth method, the InAs QDs size uniformity improves by 63% and 110% compared that of the dots grown by ordinary S-K method and ALE method, respectively. Narrow photoluminescence (PL) emission spectrum of the QDs grown by using the two-step growth method is received. FWHM of the PL curve is measured at 26 meV and the peak emission wavelength is larger than 2.3 μm at 77 K.

关键词：砷化铟量子点 MOVPE 晶体生长中红外应用

Corrosion Resistance and durability of Superhydrophobic Coating on AZ31 Mg Alloy via One-Step Electrodeposition

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Acta Metallurgica Sinica(English Letters) 2021年第1期34卷 25-38页

作者： zheng-zheng yin zhao-Qi Zhang Xiu-Juan Tian zhen-lin Wang Rong-Chang Zeng Corrosion Laboratory for Light Metals College of Materials Science and EngineeringShandong University of Science and TechnologyQingdao 266590China College of Materials Science and Engineering Chongqing University of TechnologyChongqing 400065China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450002China

To enhance durability and adhesion of superhydrophobic surface,an integrated superhydrophobic calcium myristate(Ca[CH3(CH2)12COO]2)coating with excellent corrosion resistance was fabricated on AZ31 magnesium(Mg)alloy via one-step electrodeposition ***-emission scanning electron microscopy,Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy as well as X-ray diff raction were employed to investigate the surface characteristics(morphology,composition and structure)of the *** of the coating was evaluated by means of contact and sliding ***,potentiodynamic polarization,electrochemical impedance spectroscopy and hydrogen evolution tests were conducted to characterize the corrosion *** indicated that the coating exhibited super-hydrophobicity with large static water contact angle(CA)and small sliding angle of 155.2°±1.5°and 6.0°±0.5°,respectively,owing to spherical rough structure and low surface energy(7.01 mJ m^(-2)).The average hydrogen evolution rate(HERa)and corrosion current density(icorr)of the coated sample were 5.3μL cm^(-2)h^(-1) and 5.60×10^(-9)A cm^(-2),about one and four orders of magnitude lower than that of AZ31 substrate,respectively,implying the excellent corrosion *** CA of the coating remained 155.6°±0.9°after soaking for 13 days,showing the super-hydrophobicity and stability of the ***,the large critical load(5004 mN)for the coating designated the outstanding adhesion to the substrate by nano-scratch test.

关键词： Magnesium alloy Electrodeposition durability Super-hydrophobicity Adhesion Corrosion resistance

Roles of mushroom polysaccharides in chronic disease management

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Journal of Integrative Agriculture 2022年第7期21卷 1839-1866页

作者： ZHANG Shan LEI lin ZHOU Yun YE Fa-yin zhao Guo-hua College of Food Science Southwest UniversityChongqing 400715P.R.China Chongqing Engineering Research Center of Regional Foods Chongqing 400716P.R.China

Chronic diseases have drawn much attention as the primary cause of death and disability. In exploring novel sideeffect-free agents against chronic diseases, significant efforts have been devoted to mushroom polysaccharides due to their diverse biological activities. This work reviewed the structural features, biological performances and molecular mechanisms of mushroom polysaccharides in managing cancers, diabetes mellitus and cardiovascular diseases. The potentials of mushroom polysaccharides against chronic diseases highly depend on their structural features, including monosaccharide composition, molecular weight, the type and configuration of glycosidic bonds, degree of branching, the type of substituent pattern and chain conformation. Regarding their working mechanisms, shared and diseasespecific pathways were found. The three chronic diseases shared the regulation of specific signalling pathways and the adjustment of gut microbiota. In addition, the roles of transcription factors, receptors, enzymes, hormones and other functional proteins involved in the molecular mechanisms of mushroom polysaccharides against chronic diseases are first elaborated herein. The present review describes the state of the art of mushroom polysaccharides in treating chronic diseases and addresses the perspectives, and will further promote research on this topic.

关键词： mushroom polysaccharide chronic diseases structural feature biological performance molecular mechanism

Synthesis of 3-(1-Cyclohexenyl)-2-butanone via Environmentally Friendly Catalysts

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Chemical Research in Chinese Universities 2003年第3期19卷 350-354页

作者： zhao zhen-hua Department of Applied Chemistry and Environmental Science Changsha UniversityChangsha 410003P.R.China

We substituted several environmentally friendly catalysts which included HY and H- β zeolites, various cation-exchanged β zeolites, and solid-supported ferric chloride for conventional catalysts for the synthesis of 3-(1-cyclohexenyl)-2-butanone from the reaction of ethylidenecyclohexane with acetic anhydride at room temperature. HY zeolite was found to be the most effective for this reaction, and gave the acylated product in a 72% yield under the conditions of n (ethylidenecyclohexane)/ n (acetic anhydride)/ m (HY zeolite)=1 mmol/10 mmol/0 100 g, reaction temperature 25 ℃ and reaction time 2 h. The used HY zeolite can be recovered, regenerated and gave almost the same yield as the fresh one. The lifetime of the HY zeolite is over 80 h. The effect of different factors on the reaction has also been investigated.

关键词： Acylation Zeolite Environmentally friendly catalyst 3-(1-Cyclohexenyl)-2-butanone Ethylidenecyclohexane Acetic anhydride Synthesis

Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells(bEnd.3)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第5期11卷 350-356页

作者： Yan-xia NING Shun-lin REN Feng-di zhao Lian-hua yin Department of Physiology and Pathophysiology Shanghai Medical College Fudan University Shanghai 200032 China Department of Medicine Virginia Commonwealth University Richmond Virginia 23298 USA Veterans Affairs Medical Center Richmond Virginia 23249 USA

Objective:To determine the effect of steroidogenic acute regulatory protein(StAR) overexpression on the levels of adenosine triphosphate(ATP)-binding cassette transporter A1(ABCA1) and ATP-binding cassette transporter G1(ABCG1) in an endothelial cell line(bEnd.3).Methods:The StAR gene was induced in bEnd.3 cells with adenovirus *** infection efficiency was detected by fluorescence activated cell sorter(FACS) and fluorescence *** expressions of StAR gene and protein levels were detected by real-time polymerase chain reaction(PCR) and Western *** gene and protein levels of ABCA1 and ABCG1 were detected by real-time PCR and Western blot after StAR ***:The result shows that StAR was successfully overexpressed in bEnd.3 cells by adenovirus *** mRNA and protein expressions of ABCA1 and ABCG1 were greatly increased by StAR overexpression in bEnd.3 ***:Overexpression of StAR increases ABCA1 and ABCG1 expressions in endothelial cells.

关键词： Steroidogenic acute regulatory protein(StAR) Endothelial cells Cholesterol Adenosine triphosphate (ATP)-binding cassette transporter A(ABCA) ATP-binding cassette transporter G(ABCG) bEnd. Steroidogenic acute regulatory protein(StAR) Endothelial cells Cholesterol Adenosine triphosphate (ATP)-binding cassette transporter A(ABCA) ATP-binding cassette transporter G(ABCG) bEnd.

A New Carbon and Oxygen Balance Model Based on Ecological Service of Urban Vegetation

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Chinese Geographical Science 2010年第2期20卷 144-151页

作者： yin Kai zhao Qianjun LI Xuanqi CUI Shenghui hua Lizhong lin Tao Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China Institute of Remote Sensing Applications Chinese Academy of Sciences Beijing 100101 China Institute of Spatial Information Technology Xiamen University of Technology Xiamen 361024 China

The application of human induced oxygen consumption and carbon emission theory in urban region was summed up and on this base a new model of urban carbon and oxygen balance (UCOB) was constructed by calculating the carbon and oxygen fluxes. The purpose was to highlight the role of vegetation in urban ecosystems and evaluate the effects of various human activities on urban annual oxygen consumption and carbon emission. Hopefully,the model would be helpful in theory to keep the regional balance of carbon and oxygen,and provide guidance and support for urban vegetation planning in the future. To test the UCOB model,the Jimei District of Xiamen City,Fujian Province,China,a very typical urban region,was selected as a case study. The results turn out that Jimei′s vegetation service in oxygen emission and carbon sequestration could not meet the demand of the urban population,and more than 31.49 times of vegetation area should be added to meet the whole oxygen consumption in Jimei while 9.60 times of vegetation area are needed to meet the carbon sequestration targets. The results show that the new UCOB model is of a great potential to be applied to quantitative planning of urban vegetation and regional eco-compensation mechanisms.

关键词： ecological service carbon cycle oxygen cycle urban carbon and oxygen balance urban vegetation planning

Corrosion Resistance of Superhydrophobic Mg(OH)_(2)/Calcium Myristate Composite Coating on Magnesium Alloy AZ31

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Acta Metallurgica Sinica(English Letters) 2021年第12期34卷 1618-1634页

作者： zheng-zheng yin Wei zhao Jing Xu Rong-Chang Zeng Feng-Qin Wang zhen-lin Wang Corrosion Laboratory for Light Metals College of Materials Science and EngineeringShandong University of Science and TechnologyQingdao 266590China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001China Hubei Key Laboratory of Advanced Technology for Automotive Components Wuhan 430070China College of Materials Science and Engineering Chongqing University of TechnologyChongqing 400065China

Hydrophobic Mg(OH)_(2)/calcium myristate(Ca[CH3(CH2)12COO]_(2),CaMS)and Mg(OH)_(2)/magnesium myristate(Mg[CH3(CH2)12COO]2,MgMS)composite coatings were prepared on the alkali-treated AZ31 substrates via both electrodeposition and dipping *** morphologies,compositions,and constitutes of the coatings were investigated by using field-emission scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),and Fourier transform infrared spectrometry(FTIR)together with X-ray diffractometer(XRD).Furthermore,electrochemical(polarization and impedance spectroscopy)and hydrogen evolution tests were applied to evaluate the corrosion *** wettability and adhesive strength of the composite coatings were characterized through water static contact angle(CA),sliding angle(SA),and nano-scratch *** indicated that the better super-hydrophobicity,corrosion resistance,and adhesion were achieved via an electrodeposited *** corrosion current density(icorr)and hydrogen evolution rate(HER)of the electrodeposited coating were three and one orders of magnitude smaller than the substrate,implying a significantly improved corrosion *** scenario was ascribed to the super-hydrophobicity of electrodeposited composite coating with a contact angle(CA)and slide angle(SA)of 159.2°±0.8°and 5.2°±0.8°,***,the dipped composite coating was adverse to the improvement of corrosion resistance and adhesion due to the dissolution of the underlying Mg(OH)_(2)layer and smooth surface with less organic fatty acid salt(MgMS)

关键词： Magnesium alloy Electrodeposition and dipping Super-hydrophobicity Corrosion resistance