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The foxtail millet Si69 gene is a Wali7 (wheat aluminum-induced protein 7) homologue and may function in aluminum tolerance

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Chinese Science Bulletin 2009年第10期54卷 1697-1706页

作者： zhao linna zhao qian ao guangming yu jingjuan State Key Laboratory for Agro-Biotechnology College of Biological Sciences China Agricultural University Beijing 100193 China

We isolated a clone, named Si69, from a foxtail millet immature seed cDNA library. The protein encoded by Si69 contains a conserved Wali7 (wheat aluminum induced protein 7) domain and shares high-level homology with aluminum-induced proteins from other species including rice and Arabidopsis. The Si69 gene presents as a single locus in foxtail millet genome and is globally expressed in all tissues examined. Its expression is up-regulated by aluminum. The sequence feature and expression pattern suggest that the Si69 gene is involved in aluminum tolerance or detoxification. To confirm its biological functions, Si69 controlled by the CaMV35S promoter was introduced into Arabidopsis. Transgenic plants did not show any visible morphological changes compared to wild-type plants under normal growth conditions. However, when treated with 20 or 50 μmol/L Aluminum (Al), the root apices of wild-type plants were heavily stained by hematoxylin, whereas those of Si69 transgenic plants were not stained when treated with 20 μmol/L Al and slightly stained when treated with 50 μmol/L Al. Scanning electron microscopy (SEM) results further demonstrated that the damage of the root apices was se- verer in wild-type plants than in transgenic plants. Inhibition of root growth and accumulation of malondialdehyde (MDA), an indicator of lipid peroxidation, were lower in transgenic plants than in wild-type plants. The results show that overexpression of Si69 may increase Al tolerance in transgenic plants, indicating that a series of Wali7-containing genes may play similar roles in Al tolerance/detoxification.

关键词： Si69 诱导蛋白耐铝性基因组同源性谷子小麦转基因植物

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Ectopic expression of foxtail millet zip-like gene,SiPf40,in transgenic rice plants causes a pleiotropic phenotype affecting tillering,vascular distribution and root development

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Science China(Life Sciences) 2010年第12期53卷 1450-1458页

作者： LUAN yunXia WANG BaoSheng zhao qian ao guangming yu jingjuan State Key Laboratory for Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193China National Engineering Research Center for Information Technology in Agriculture Beijing 100097China

Plant architecture determines grain production in rice(Oryza sativa) and is affected by important agronomic traits such as tillering,plant height,and panicle *** key genes involved in controlling the initiation and outgrowth of axillary buds,the elongation of stems,and the architecture of inflorescences have been isolated and *** studies have shown that SiPf40,which was identified from a foxtail millet(Setaria italica) immature seed cDNA library,causes extra branches and tillers in SiPf40-transgenic tobacco and foxtail millet,*** reconfirm its function,we generated transgenic rice plants overexpressing SiPf40 under the control of the ubiquitin ***40-overexpressing transgenic plants have a greater tillering number and a wider tiller angle than wild-type *** root architecture is modified by the promotion of lateral root development,and the distribution of xylem and phloem in the vascular bundle is *** of hormone levels showed that the ratios of indole-3-acetic acid/zeatin(IAA/ZR) and IAA/gibberellic acid(IAA/GA) decreased in SiPf40-transgenic plants compared with wild-type *** findings strongly suggest that SiPf40 plays an important role in plant architecture.

关键词： SiPf40 ZIP phytohormone plant architecture root development tiller vascular rice

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Functional Characterization of Pollen Specific non-coding mRNA-like RNA Gene zm401 from Zea mays

Functional Characterization of Pollen Specific non-coding mR...

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中国遗传学会植物遗传与基因组学专业委员会2005年学术研讨会

作者： Dai Xiaoyan Majinxia yu jingjuan zhao qian ao guangming(Stale Key Laboratory for Agricultural Biotechnology, College of Biology, China Agricultural University,Beijing 100094, China)State Key Laboratory for AgrobiotechnologyDepartment of Biochemistry and Molecular BiologyCollege of Biological ScienceChina Agricultural Universit

By differential screening of pollen cDNA library of Zea mays, we obtained nine clones. The full length of one of these clones, designed zm401, was obtained by 5’ RACE. Within the full-length cDNA sequence, a clear open reading frame (ORF) was undectable by omega 2.0 and DNAMAN software. The longest potential ORF was 269 nucleotides long (791-1060), coding 89 AA, and it had a poly (A) tail. This indicate that zm401 was probably one of a growing number of non-coding mRNA-like RNA transcripts that exert their cellular functions directly as RNA. RT-PCR and Northern blot analyses showed the zm401 was transcribed from tetrad stage of microspore development and increased in concentration up to mature pollen. This suggests that zm401 likely functions in pollen growth and/or development. For analysis of the function of zm401 was ectopically expressed in tobacco and overexpressed in maize under the control of a pollen specific promoter zm13. Interestingly, all transgenic tobacco and maize plants showed various levels of sterility, ranging from abortive flower development to male sterility. Further analyses on anther development indicated multiple abnormalities of anther development at late stages. These abnormalities include lagged degradation of the tapetum and connective tissue, failed deposition of fibrous bands in endothecial cells, and aborted pollen grain development. These strongly suggest that zm401 has essential roles in anther development and its ability to perturb the anther development in heterogeneous dicotyledonous tobacco and maize indicates that zm401 functions in a molecular mechanism possibly conserved in both monocots and dicots.

关键词： Anther Development Pollen-specific Gene Ectopic expression maize tobacco overexpression

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Zm401p10,encoded by an anther-specific gene with short open reading frames,is essential for tapetum degeneration and anther development in maize

Zm401p10,encoded by an anther-specific gene with short open ...

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中国生物化学与分子生物学会农业生物化学与分子生物学分会第八次学术研讨会

作者： Dongxue Wang~1 Chengxia Li~2 qian zhao Dengyun Zhu guangming ao jingjuan yu~* State Key Laboratory for Agro-biotechnology,College of Biological Sciences,China Agricultural University,No.2 yuanmingyuan West Road,Beijing 100094,P.R.China

In flowering plants,the tapetum is proposed to play a vital role at the early stages of pollen *** in tapetum development and degeneration typically result in male ***,we report the characterization of a maize (Zea mays L.) anther-specific gene,Zm401,which only contains short open reading frames (sORFs).The longest ORF of the Zm401 gene encodes a small protein designated Zm401p10 that accumulates in the nucleus. The over expression of Zm401p10 in maize retarded tapetal degeneration and caused microspore *** microarray analysis,we identified 278 downregulated and 150 upregulated genes in anthers over expressing *** results indicate that the Zm401 gene is one of major components of the molecular network regulating maize anther development and male fertility,and that Zm401p10 is expressed from the longest of the sORFs of the gene.

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用基因枪将高赖氨酸基因导入玉米及转基因植株的检测

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农业生物技术学报 1999年第4期7卷 363-367页

作者：张秀君刘俊起赵倩于静娟敖光明中国农业大学生物学院!北京 100094

构建了两个含高赖氨酸蛋白质基因cDNA的表达载体，用基因枪法将其导入玉米不同杂交组合的胚性愈伤组织，经潮霉素抗性筛选，得到了可育的再生植株，经PCR扩增分析、点杂交、及Southern杂交检测，表明该基因已整合到玉米的基因组中。测... 详细信息

构建了两个含高赖氨酸蛋白质基因cDNA的表达载体，用基因枪法将其导入玉米不同杂交组合的胚性愈伤组织，经潮霉素抗性筛选，得到了可育的再生植株，经PCR扩增分析、点杂交、及Southern杂交检测，表明该基因已整合到玉米的基因组中。测定13株T1代种子中赖氨酸的含量，其中有3株赖氨酸含量提高10％以上。

关键词：转化基因枪赖氨酸玉米

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