检索结果-南通市图书馆

菌株J1对铜绿微囊藻光合作用的影响及其溶藻活性成分的研究

在线全文

学校读者我要写书评

暂无评论

环境科学研究 2010年第6期23卷 685-689页

作者：晋利汪辉赵耕毛陈雷刘兆普南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室江苏南京210095

利用浮游植物荧光仪研究了芽胞杆菌属菌株J1胞外溶藻活性物质对铜绿微囊藻叶绿素荧光活性的影响,并采用α-萘酚反应、考马斯亮蓝法和定磷法对无菌滤液中溶藻活性物质进行了初步判定,结合蛋白酶处理、透析、活性炭吸附与解吸的方法对其... 详细信息

利用浮游植物荧光仪研究了芽胞杆菌属菌株J1胞外溶藻活性物质对铜绿微囊藻叶绿素荧光活性的影响,并采用α-萘酚反应、考马斯亮蓝法和定磷法对无菌滤液中溶藻活性物质进行了初步判定,结合蛋白酶处理、透析、活性炭吸附与解吸的方法对其相关特性进行了研究.结果表明:试验第9天,加入无菌滤液的处理组,反映铜绿微囊藻叶绿素荧光活性的参数显著下降,其最大电子相对传递速率,光能转化效率,PSⅡ潜在活性和PSⅡ实际光合效率分别是对照的0.22%,0.33%,4.37%和5.56%;J1的溶藻活性物质可能为糖类物质,但不是蛋白质和核酸类物质,其分子质量小于8 ku,可被活性炭吸附.

关键词：叶绿素荧光溶藻活性物质蛋白酶K 透析活性炭吸附与解吸

First experimental constraints on WIMP couplings in the effective field theory framework from CDEX

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2021年第8期64卷 15-22页

作者： Yi Wang Zhi Zeng Qian Yue LiTao Yang KeJun Kang YuanJing Li Mehment Agartioglu HaiPeng An JianPing Chang JingHan chen YunHua chen JianPing cheng cheng Yi Chiang WenHan dai Zhi Deng ChangHao Fang XinPing geng Hui Gong QiuJu guo XuYuan guo HongJian He Li He Shengming He Jinwei Hu Tuchen Huang HanXiong Huang HaiTao Jia LiPing Jia Xi Jiang HauBin Li JianMin Li Jin Li mingXuan Li RenmingJie Li Xia Li YuLan Li Bin Liao FongKay Lin shinTed Lin ShuKui liu YanDong liu Yu liu YuanYuan liu ZhongZhi liu Hao Ma YuCai mao QiYuan Nie JinHua Ning Hui Pan NingChun Qi Jie Ren XiChao Ruan ChangSong Shang Vivek Sharma Ze She Lakhwinder Singh Monoj Kumar Singh TianXi Sun ChangJian Tang weiYou Tang Yang Tian GuangFu Wang Li Wang Qing Wang Yu chen Wang YunXiang Wang Zhen Wang Henry Tsz-King Wong shiYong Wu Yucheng Wu HaoYang Xing Yin Xu Tao Xue YuLu Yan Nan Yi ChunXu Yu HaiJun Yu JianFeng Yue ming Zeng BingTao Zhang Lei Zhang FengShou Zhang ZhenYu Zhang KangKang zhao mingGang zhao JiFang Zhou ZuYing Zhou JingJun Zhu Key Laboratory of Particle and Radiation Imaging(Ministry of Education) Department of Engineering PhysicsTsinghua UniversityBeijing 100084China Department of Physics Tsinghua UniversityBeijing 100084China Institute of Physics Academia SinicaTaipei 11529China NUCTECH Company Beijing 100084China YaLong River Hydropower Development Company Chengdu 610051China College of Nuclear Science and Technology Beijing Normal UniversityBeijing 100875China College of Physics Sichuan UniversityChengdu 610065China School of Physics Peking UniversityBeijing 100871China Sino-French Institute of Nuclear and Technology Sun Yet-sen UniversityZhuhai 519082China Department of Nuclear Physics China Institute of Atomic EnergyBeijing 102413China Department of Physics Banaras Hindu UniversityVaranasi 221005India Department of Physics Beijing Normal UniversityBeijing 100875China School of Physics Nankai UniversityTianjin 300071China

We present weakly interacting massive particles(WIMPs) search results performed using two approaches of effective field theory from the China dark Matter Experiment(CDEX), based on the data from both CDEX-1B and CDEX-10 stages. In the nonrelativistic effective field theory approach, both time-integrated and annual modulation analyses were used to set new limits for the coupling of WIMP-nucleon effective operators at 90% confidence level(C.L.) and improve over the current bounds in the low mχregion. In the chiral effective field theory approach, data from CDEX-10 were used to set an upper limit on WIMP-pion coupling at 90% C.L. We for the first time extended the limit to the m_(χ)<6 GeV/c^(2) region.

关键词： WIMP EFT CDEX dark matter annual modulation

Constraining self-interacting dark matter with the full dataset of PandaX-II

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2021年第11期64卷 2-7页

作者： Jijun Yang Abdusalam Abdukerim wei chen Xun chen Yunhua chen chen cheng Xiangyi Cui Yingjie Fan Deqing Fang Changbo Fu Mengting Fu Lisheng geng Karl Giboni Linhui Gu Xuyuan guo Ke Han Changda He Shengming He Di Huang Yan Huang Ran Huo Yanlin Huang Zhou Huang Xiangdong Ji Yonglin Ju Shuaijie Li Qing Lin Huaxuan liu Jianglai liu Xiaoying Lu Wenbo Ma Yugang Ma Yajun mao Yue Meng Nasir Shaheed Kaixiang Ni Jinhua Ning Xuyang Ning Xiangxiang Ren Changsong Shang guofang Shen Lin Si Andi Tan Anqing Wang Hongwei Wang Meng Wang QiuHong Wang Siguang Wang wei Wang Xiuli Wang Zhou Wang Mengmeng Wu shiyong Wu weihao Wu Jingkai Xia Mengjiao Xiao Xiang Xiao Pengwei Xie Binbin Yan Yong Yang Chunxu Yu Hai-Bo Yu Jumin Yuan Ying Yuan Xinning Zeng dan Zhang Tao Zhang Li zhao Qibin Zheng Jifang Zhou Ning Zhou Xiaopeng Zhou School of Physics and Astronomy Shanghai Jiao Tong UniversityMOE Key Laboratory for Particle Astrophysics and CosmologyShanghai Key Laboratory for Particle Physics and CosmologyShanghai 200240China Shanghai Jiao Tong University Sichuan Research Institude Chengdu 610213China Yalong River Hydropower Development Company Ltd.Chengdu 610051China School of Physics Sun Yat-sen UniversityGaungzhou 510275China Tsung-dao Lee Institute Shanghai 200240China School of Physics Nankai UniversityTianjin 300071China Key Laboratory of Nuclear Physics and Ion-beam Application(MOE) Institute of Modern PhysicsFudan UniversityShanghai 200433China School of Physics Peking UniversityBeijing 100871China School of Physics Beihang UniversityBeijing 100191China International Research Center for Nuclei and Particles in the Cosmos&Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang UniversityBeijing 100191China Shandong Institute of Advanced Technology Jinan 250103China School of Medical Instrument and Food Engineering University of Shanghai for Science and TechnologyShanghai 200093China Department of Physics University of MarylandCollege ParkMaryland 20742USA School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China Key Laboratory of Particle Physics and Particle Irradiation of Ministry of Education Shandong UniversityJinan 250100China Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Department of Physics and Astronomy University of CaliforniaRiversideCalifornia 92507USA Center of High Energy Physics Peking UniversityBeijing 100871China

Self-interacting dark matter(SIDM)is a leading candidate proposed to solve discrepancies between predictions of the prevailing cold dark matter theory and observations of *** SIDM models predict the existence of a light force carrier that mediates strong dark matter *** the mediator couples to the standard model particles,it could produce characteristic signals in dark matter direct detection *** report searches for signals of SIDM models with a light mediator using the full dataset of the PandaX-II experiment,basing on a total exposure of 132 *** significant excess over background is found,and our likelihood analysis leads to a strong upper limit on the dark matter-nucleon coupling *** further combine the PandaX-II constraints and those from observations of the light element abundances in the early universe,and show that direct detection and cosmological probes can provide complementary constraints on dark matter models with a light mediator.

关键词： dark matter direct detection self-interacting dark matter

在线全文

学校读者我要写书评

暂无评论

氮磷水平对龙须菜生长和光合特性的影响

植物生态学报 2009年第6期33卷 1140-1147页

作者：李枫邹定辉刘兆普赵耕毛程丽巍朱喜锋陈伟洲南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室南京210095 华南理工大学环境科学与工程学院广州510006 汕头大学海洋生物研究所广东汕头515063

研究不同营养盐条件对龙须菜(Gracilaria lemaneiformis)的生理效应,对深入了解龙须菜与近海环境的相互作用具有重要意义。在低氮低磷(LNLP)、低氮高磷(LNHP)、高氮低磷(HNLP)和高氮高磷(HNHP)4种营养盐条件下培养龙须菜15d,以探讨不同... 详细信息

研究不同营养盐条件对龙须菜(Gracilaria lemaneiformis)的生理效应,对深入了解龙须菜与近海环境的相互作用具有重要意义。在低氮低磷(LNLP)、低氮高磷(LNHP)、高氮低磷(HNLP)和高氮高磷(HNHP)4种营养盐条件下培养龙须菜15d,以探讨不同氮、磷水平对龙须菜生长和光合特性的影响。结果表明:1)LNHP、HNLP和HNHP处理促进了龙须菜的生长,其中HNHP处理下龙须菜具有最大的相对生长速率和生物量;2)LNHP、HNLP和HNHP处理提高了龙须菜的光合无机碳利用能力,其中HNHP处理下龙须菜具有最大的无机碳饱和光合速率和表观半饱和常数,比LNLP处理分别提高了118%倍和48.71%;3)LNHP、HNLP和HNHP处理显著影响龙须菜的光化学效率,与LNLP处理相比,LNHP处理提高了龙须菜的光化学效率,而HNLP和HNHP处理降低了龙须菜的光化学效率。研究结果表明,HNHP处理条件下,龙须菜的生长和光合无机碳利用能力最高,光化学效率最低。

关键词：龙须菜氮磷生长光合作用光化学效率

在线全文

学校读者我要写书评

暂无评论

不同气候带海水灌溉下滨海盐土水盐运动特征

水土保持学报 2004年第1期18卷 43-46,50页

作者：刘兆普赵耕毛刘玲陈铭达刘联綦长海南京农业大学资源与环境科学学院南京210095 南京农业大学海南滩涂农业研究所海南乐东572541 山东莱州益丰盐生植物有限公司山东莱州261418

自2001年开始,采用田间试验与室内模拟相互验证的方法,对山东莱州(暖温半干旱带)、江苏大丰(暖温湿润带)、海南乐东(热带湿润带)进行了海水灌溉下滨海盐土水盐运动特征研究,结果表明:(1)各气候带适宜的灌溉定额下,未作任何人为调控措施... 详细信息

自2001年开始,采用田间试验与室内模拟相互验证的方法,对山东莱州(暖温半干旱带)、江苏大丰(暖温湿润带)、海南乐东(热带湿润带)进行了海水灌溉下滨海盐土水盐运动特征研究,结果表明:(1)各气候带适宜的灌溉定额下,未作任何人为调控措施,半干旱的莱州虽呈自然积盐的趋势,但即使用50%的海淡水混灌,2年0～60cm土层盐分仍未超过3‰,江苏大丰用60%的海淡水灌溉,海水灌溉携入的总盐量与1m土体淋失的盐量持平,而海南乐东盐分收支平衡的海淡水比例为75%;(2)在半干旱的山东莱州,0～60cm各土层含盐量变幅起伏较大,表明蒸发为盐分运动的控制因子,而在湿润的江苏大丰,30cm以下土层含盐量平稳,地下水控制盐分运动的作用增强。两个气候带,在整个夏季,各海水灌溉处理表层土壤含盐量明显低于下层土壤;(3)海水灌溉明显降低土壤水吸力,表明海水灌溉可以缓解土壤水的蒸发,越是干旱,这种作用越明显。

关键词：气候带海水灌溉滨海盐土水盐运动

在线全文

学校读者我要写书评

暂无评论

沙质海滩海水连续灌溉对芦荟生长发育的影响

干旱地区农业研究 2006年第6期24卷 35-38,63页

作者：王学勤刘兆普赵耕毛郑青松陈健妙云燕符永升刘玲北京大学环境学院北京100871 南京农业大学资源与环境科学学院江苏南京210095 海南省农业科学研究院海南海口571100 南京农业大学(海南)滩涂农业研究所海南乐东572541

海南海涂沙滩(非耕地)库拉索芦荟从苗期至叶片采摘期连续3 a的海水小区灌溉试验结果表明:①不同生长发育期海水灌溉对芦荟地上部产量影响不同,2000年11月至2003年3月(苗期),10%、25%海水两处理地上部产量(干物质,下同)同淡水灌溉处理(CK... 详细信息

海南海涂沙滩(非耕地)库拉索芦荟从苗期至叶片采摘期连续3 a的海水小区灌溉试验结果表明:①不同生长发育期海水灌溉对芦荟地上部产量影响不同,2000年11月至2003年3月(苗期),10%、25%海水两处理地上部产量(干物质,下同)同淡水灌溉处理(CK,下同)没有显著差异,从50%海水处理开始,随着海水比例的增加,库拉索芦荟地上部产量显著下降;随着灌溉时间的延长,至芦荟叶片采收期(2004年5月),10%、25%、50%三个处理与CK比地上部产量没有显著差异,而从75%海水处理起,其地上部产量开始显著下降。②海水灌溉芦荟幼苗始期(2000年11月初至2001年1月底),与CK比较,所有比例的海水处理芦荟根的生长量(干重,下同)均显著下降,随着灌溉时间的延长(至2003年3月),从75%海水灌溉处理起,其根生长量开始显著下降,而且25%海水处理芦荟根的生长量显著高于CK,至2004年5月的叶片采收期,75%、100%比例海水处理芦荟根的生长量显著低于CK,而其它处理与CK没有差异。③土壤各层次含盐量在2000年11月至2003年3月的灌溉处理期间内,无规律性的变化,而至2004年5月,无论是耕层土,还是底层土,随着海水比例的增加,盐分含量呈显著增加的趋势。但即使全海水灌溉,土壤含盐量仍在0.2%以下。④土壤各灌溉处理速效磷变化差异不显著,总体上速效磷水平较低;海水灌溉显著提高了耕层土的速效钾含量,而CK处理中,耕层土速效钾含量在20 mg/kg以下。

关键词：沙质海滩海水连续灌溉库拉索芦荟生长量土壤含盐量

Reconstruction of Cherenkov image bymultiple telescopes of LHAASO-WFCTA

在线全文

学校读者我要写书评

暂无评论

Radiation Detection Technology and Methods 2022年第4期6卷 544-557页

作者： F.Aharonian Q.An Axikegu L.X.Bai Y.X.Bai Y.W.Bao D.Bastieri X.J.Bi Y.J.Bi J.T.Cai Zhe Cao Zhen Cao J.Chang J.F.Chang E.S.chen Liang chen Liang chen Long chen M.J.chen M.L.chen Q.H.chen S.H.chen S.Z.chen T.L.chen Y.chen H.L.cheng N.cheng Y.D.cheng S.W.Cui X.H.Cui Y.D.Cui B.D’Ettorre Piazzoli B.Z.dai H.L.dai Z.G.dai danzengluobu Ddella Volpe K.K.Duan J.H.Fan Y.Z.Fan Z.X.Fan J.Fang K.Fang C.F.Feng L.Feng S.H.Feng X.T.Feng Y.L.Feng B.Gao C.D.Gao L.Q.Gao Q.Gao W.Gao W.K.Gao M.M.Ge L.S.geng G.H.Gong Q.B.Gou M.H.Gu F.L.guo J.G.guo X.L.guo Y.Q.guo Y.Y.guo Y.A.Han H.H.He H.N.He S.L.He X.B.He Y.He M.Heller Y.K.Hor C.Hou X.Hou H.B.Hu Q.Hu S.Hu S.C.Hu X.J.Hu D.H.Huang W.H.Huang X.T.Huang X.Y.Huang Y.Huang Z.C.Huang X.L.Ji H.Y.Jia K.Jia K.Jiang Z.J.Jiang M.Jin M.M.Kang T.Ke D.Kuleshov K.Levochkin B.B.Li cheng Li Cong Li F.Li H.B.Li H.C.Li H.Y.Li J.Li Jian Li Jie Li K.Li WLLi XRLi Xin Li Xin Li YZLi Zhe Li Zhuo Li E.W.Liang Y.F.Liang S.J.Lin B.liu C.liu D.liu H.liu H.D.liu J.liu J.L.liu J.S.liu J.Y.liu M.Y.liu R.Y.liu S.M.liu W.liu Y.liu Y.N.liu W.J.Long R.Lu Q.Luo H.K.Lv B.Q.Ma L.L.Ma X.H.Ma J.R.mao A.Masood Z.Min W.Mitthumsiri Y.C.Nan Z.W.Ou B.Y.Pang P.Pattarakijwanich Z.Y.Pei M.Y.Qi Y.Q.Qi B.Q.Qiao J.J.Qin D.Ruffolo A.Sáiz C.Y.Shao L.Shao O.Shchegolev X.D.Sheng J.Y.shi H.C.Song Yu.V.Stenkin V.Stepanov Y.Su Q.N.Sun X.N.Sun Z.B.Sun P.H.T.Tam Z.B.Tang W.W.Tian B.D.Wang C.Wang H.Wang H.G.Wang J.C.Wang J.S.Wang L.P.Wang L.Y.Wang R.Wang R.N.Wang W.Wang X.G.Wang X.Y.Wang Y.Wang Y.D.Wang Y.J.Wang Y.P.Wang Z.H.Wang Z.X.Wang Zhen Wang Zheng Wang D.M.wei J.J.wei Y.J.wei T.Wen C.Y.Wu H.R.Wu S.Wu X.F.Wu Y.S.Wu S.Q.Xi J.Xia J.J.Xia G.M.Xiang D.X.Xiao G.Xiao G.G.Xin Y.L.Xin Y.Xing Z.Xiong D.L.Xu R.X.Xu L.Xue D.H.Yan J.Z.Yan C.W.Yang F.F.Yang H.W.Yang J.Y.Yang L.L.Yang M.J.Yang R.Z.Yang S.B.Yang Y.H.Yao Z.G.Yao Y.M.Ye L.Q.Yin N.Yin X.H.You Z.Y.You Y.H.Yu Q.Yuan H.Yue H.D.Zeng T.X.Zeng W.Zeng Z.K.Zeng M.Zha X.X.Zhai B.B.Zhang F.Zhang H.M.Zhang H.Y.Zhang J.L.Zhang L.X.Zhang Li Zhang Lu Zhang P.F.Zhang P.P.Zhang R.Zhang S.B.Zhang S.R.Zhang S. Key Laboratory of Particle Astrophyics&Experimental Physics Division&Computing Center Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China TIANFU Cosmic Ray Research Center ChengduSichuanChina Dublin Institute for Advanced Studies 31 Fitzwilliam Place2DublinIreland Max-Planck-Institut for Nuclear Physics P.O.Box 10398069029 HeidelbergGermany State Key Laboratory of Particle Detection and Electronics BeijingChina University of Science and Technology of China Hefei 230026AnhuiChina School of Physical Science and Technology&School of Information Science and Technology Southwest Jiaotong UniversityChengdu 610031SichuanChina College of Physics Sichuan UniversityChengdu 610065SichuanChina School of Astronomy and Space Science Nanjing UniversityNanjing 210023JiangsuChina Center for Astrophysics Guangzhou UniversityGuangzhou 510006GuangdongChina Key Laboratory of dark Matter and Space Astronomy&Key Laboratory of Radio Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210023JiangsuChina Key Laboratory for Research in Galaxies and Cosmology Shanghai Astronomical ObservatoryChinese Academy of SciencesShanghai 200030China Key Laboratory of Cosmic Rays(Tibet University) Ministry of EducationLhasa 850000TibetChina National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Hebei Normal University Shijiazhuang 050024HebeiChina School of Physics and Astronomy(Zhuhai)&Sino-French Institute of Nuclear Engineering and Technology(Zhuhai) Sun Yat-sen UniversityZhuhai 519000China School of Physics(Guangzhou) Sun Yat-sen UniversityGuangzhou 510275GuangdongChina Dipartimento di Fisica dell’Universitàdi Napoli“Federico II” Complesso Universitario di Monte Sant’Angelovia Cinthia80126 NapoliItaly School of Physics and Astronomy Yunnan UniversityKunming 650091YunnanChina Département de Physique Nucléaire et Corpusculaire Facultéde SciencesUniversitéde Genève

Introduction One of main scientiﬁc goals of the Large High Altitude Air Shower Observatory(LHAASO)is to accurately measure the energy spectra of different cosmic ray compositions around the‘knee’*** Wide Field-of-View(FoV)Cherenkov Telescope Array(WFCTA),which is one of the main detectors of LHAASO and has 18 telescopes,is built to achieve this *** telescopes are put together and point to connected directions for a larger *** Telescopes are deployed spatially as close as possible,but due to their own size,the distance between two adjacent telescopes is about 10 ***,the Cherenkov lateral distribution and the parallax between the two telescopes should be considered in the event building process for images crossing over the boundaries of FoVs of the *** event building method for Cherenkov images measured by multiple telescopes of WFCTA is *** performance of the shower measurements using the combined images is evaluated by comparing with showers that are fully contained by a virtual telescope in *** and conclusion It is proved that the developed event building process can help to increase the FoV of WFCTA by 30%while maintaining the same reconstruction quality,compared to the separate telescope reconstruction method.

关键词： method directions conclusion

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BES-Ⅱ TOF数据的离线刻度

高能物理与核物理 2001年第2期25卷 154-159页

作者：荣刚赵家伟刘琦薛生田陈光培李佩琴刘靖毛泽普李卫国邱进发徐春成谢跃红袁野赵海文中国科学院高能物理研究所北京100039 中国科学技术大学近代物理系合肥230026

简要地讨论了北京谱仪－Ⅱ（ＢＥＳ－Ⅱ）飞行时间计数器（ＴＯＦ）数据的离线刻度．利用Ｊ／φ能区的电子对事例仔细完成的离线刻度与校正结果表明，新的ＴＯＦ时间分辨达到σＴＯＦ＝１８０ｐｓ．由此大大增强了对带电粒子的识别能... 详细信息

简要地讨论了北京谱仪－Ⅱ（ＢＥＳ－Ⅱ）飞行时间计数器（ＴＯＦ）数据的离线刻度．利用Ｊ／φ能区的电子对事例仔细完成的离线刻度与校正结果表明，新的ＴＯＦ时间分辨达到σＴＯＦ＝１８０ｐｓ．由此大大增强了对带电粒子的识别能力．对于３σ的π／Ｋ分离其动量可以达到０．９３ＧｅＶ／ｃ．

关键词：北京谱仪飞行时间计数器数据离线刻度时间分辨本领粒子识别

转录因子T-bet/GATA3在EV71感染患儿外周血单个核细胞中的表达及其与相关细胞因子的关系

在线全文

学校读者我要写书评

暂无评论

中华疾病控制杂志 2011年第7期15卷 575-578页

作者：耿建忠柳迎昭石燕苏兆亮茅凌翔陈建国江苏大学附属人民医院儿科江苏镇江212002 江苏大学附属人民医院检验科江苏镇江212002 江苏大学基础医学与医学技术学院江苏镇江212013 镇江市疾病预防控制中心江苏镇江212001

目的探讨转录因子T-bet/GATA3在肠道病毒71型(enterovirus 71,EV71)感染患儿外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达及其与相关细胞因子的关系。方法收集42例EV71感染患者外周血标本,其中急性期28例、恢复... 详细信息

目的探讨转录因子T-bet/GATA3在肠道病毒71型(enterovirus 71,EV71)感染患儿外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达及其与相关细胞因子的关系。方法收集42例EV71感染患者外周血标本,其中急性期28例、恢复期14例,另外健康对照组21例。采用实时荧光定量PCR(QRT-PCR)检测PBMC中T-bet和GATA3 mRNA水平;酶联免疫吸附(ELISA)法检测血浆IFN-γ和IL-4的蛋白浓度。结果急性EV71感染患者PBMC中的T-bet mRNA表达和血浆IFN-γ水平明显低于健康对照组(P<0.05),急性EV71感染患者GATA3 mRNA的表达和IL-4水平则明显高于健康对照组(P<0.05)。结论急性EV71感染患者存在Th2优势分化,可能与转录因子T-bet表达下调、GATA3表达上调有关。监测EV71感染患者外周血Th1/Th2细胞特异性转录因子与相关细胞因子的水平,有助于对病情的判断。

关键词：肠道病毒A型人转录因子病例对照研究