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Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer

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Science china(Life Sciences) 2006年第2期49卷 164-171页

作者： zhang yunhai1. pan dengke1.2, SUN Xiuzhu1. SUN Guojie1. WANG Xiaobo1. LIU Xiaohui1. LI Yan1. DAI Yunping1.& LI Ning1.1. State Key Laboratory for Agrobiotechnology,{1., beijing 1.0094, china 2. College of Animal Science and Technology,{1., beijing 1.0094, china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China College of Animal Science Technology China Agricultural University Beijing 100094 China

In the present study, nuclear transferred embryos (NTEs) were reconstructed by using pig fetal fibroblasts as donors; in vitro matured oocytes as recipients. The effects of G41. selection on donor cells, duration of IVM of prepubertal gilt oocytes; oxygen tension in IVM of oocytes were investigated. The results were as follows: (i) When G41. selected cells expressing GFP were used as donors, the cleavage rate of NTEs decreased drastically in comparison to NTEs derived from donors without antibiotic selection (47.5% vs. 71.6%, p>0.05). For the blastocyst rate, no significant difference was observed between two groups (1.% vs. 1..4%, p0.05). However, no statistical difference was observed between NTEs derived from oocytes of 36 h IVM group; NTEs from oocytes of 42 h IVM group in the rates of cleavage (59.3% vs. 73.6%, p1..2%, p1., p1..8% vs. 1..3%, p1. selection does not have a significant effect on cleavage rate of NTEs expressing GFP. (ii) Nuclear maturation is greatly improved by prolonging IVM duration from 36 to 42 h, while no significant differences were observed for developmental potential of transgenic embryos. Thus IVM 42 h is the better choice in order to obtain maximum number of MII oocytes as recipients. (iii) Lower oxygen tension; higher oxygen tension in IVM have no significant effect on development of cloned embryos.

关键词： transgenic cloning, GFP, Pig, early development.

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Seasonal influence on the development of parthenotes and cloned embryos in pigs

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Progress in Natural Science:Materials International 2007年第10期17卷 1172-1176页

作者： zhang Kun1, zhang yunhai3,4, pan dengke5, Ma Yufang1, Wei Hengxi1, zhang Jian6, Li Xuyang7, Wang Shaohua1, Li Yan2, Dai Yunping1.2 and Li Ning1(1. State Key Laboratory for Agrobiotechnology, College of Biological Science, china agricultural university, beijing 1.0094, china 2. JiPulin Biotech Ltd., beijing 1.0094, china 3. Danish Institute of {1.} Sciences, 8830 Tjele, Denmark 4. College of Animal Science and Technology, Anhui {1.} {1.}, Hefei 230036, china 5. Institute of Animal Sciences, Chinese Academy of {1.} Sciences, beijing 1.0094, china 6. Animal Clinical Hospital, china agricultural university, beijing 1.0094, china 7. Hebei Minghui Group of Pigs Breeding, Sanhe 065200, china) State Key Laboratory for Agrobiotechnology College of Biological Science China Agricultural University Beijing 100094 China JiPulin Biotech Ltd. Beijing 100094 China Danish Institute of agricultural Sciences 8830 Tjele Denmark College of Animal Science and Technology Anhui Agricultural University Hefei 230036 China Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China 7. Hebei Minghui Group of Pigs Breeding Sanhe 065200 China

Seasonal influence on the developmental ability of porcine embryos was studied by comparisons of the number of cumulus-oocytes-complexes (COCs) per ovary pair and meiotic maturation ability of oocytes in different seasons; and by the observations on developmental competence of the parthenogenetic oocytes and cloned embryos collected from different seasons. We found that the number of COCs per ovary pairs was significantly higher in spring compared to the other seasons (P<0.05). However, no significant difference was found in the rate of oocytes progressing to MⅡ in four seasons (P<0.05). In addition, the rate of blastocyst formation of parthenogenetic embryos in summer was obviously declined compared to the other seasons (P<0.05), and an increased blastocyst rate of cloned porcine embryos was found in spring compared to autumn and winter (P<0.05). The results suggest that there should be a seasonal influence on the developmental competence of porcine embryos.

关键词： pig cloned parthenogenetic season

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Spatiotemporal distribution,environmental risk and carbon emission reduction potential of livestock manure in Shaanxi Province,china

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Frontiers of agricultural Science and Engineering 2024年第3期11卷 381-396页

作者： Mengyuan QIAO Jianchun ZHU Ronghua LI Junting pan Ran XIAO Hailong WANG Kuok Ho Daniel TANG Zengqiang {1. College of Humanities&Social Development Northwest A&F UniversityYangling 712100China College of Natural Resources and Environment Northwest A&F UniversityKey Laboratory of Low-carbon Green Agriculture in Northwestern ChinaMinistry of Agriculture and Rural AffairsYangling 712100China Key Laboratory of Non-point Source Pollution of Ministry of agricultural and Rural Affairs Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin College of Resources and EnvironmentSouthwest UniversityChongqing 400715China Biochar Engineering Technology Research Center of Guangdong Province School of Environmental and Chemical EngineeringFoshan UniversityGuangdong 528000China Department of Environmental Science University of ArizonaTucsonAZ 85721USA

Shaanxi is a leading province in animal husbandry(AH)in ***,the lack of provincial information on the characteristics and utilization potential of livestock manure(LM)hinders crucial management ***,we investigated the spatiotemporal distribution,availability and biogas potential of LM in Shaanxi,and examine the carbon emission reduction potential of *** has been a 1.26-fold increase in LM quantities in Shaanxi over the past 35 years,reaching 4635.6×1.^(4)t by *** was mainly concentrated in northern Shaanxi and the eastern part of *** and pig manure were the primary sources of LM,with the average LM land-load of 1..57 t·ha^(−1.in *** the overall AH in Shaanxi has not exceeded the environmental capacity,the actual scales of AH in Ankang and Hanzhong have already surpassed the respective environmental capacities,posing a higher risk of N and P *** 2021.the estimated biogas energy potential of LM was 1.2×1.^(1.)*** 201. to 2021.the average carbon emission reduction potential in Shaanxi was 22%,with an average potential scale of 1.%.The results of this research provide valuable data and policy recommendations for promoting the intensive use of LM and reducing carbon emissions in Shaanxi.

关键词： Livestock manure emission estimation N and P loads biogas potential carbon emission reduction

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哈密绿洲土地利用变化对区域环境的影响

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干旱区研究 2018年第3期35卷 568-578页

作者：杜皓阳胡琪张弛邱源邵华潘涛金晓龙常红中国科学院新疆生态与地理研究所荒漠与绿洲国家重点实验室新疆乌鲁木齐830011 中国科学院大学北京100049 内布拉斯加大学林肯分校自然资源学院和地球与大气科学系美国林肯68583 石河子大学新疆石河子832000

中国干旱区绿洲在过去20 a经历了快速的城市化和农田扩张,为了探究该区域不同下垫面在＂荒漠-绿洲-城市＂演化过程中对区域自然环境以及气象要素的影响。以典型的哈密绿洲为研究区,利用WRF模式分别耦合2000年、201.年2期土地利用数据,... 详细信息

中国干旱区绿洲在过去20 a经历了快速的城市化和农田扩张,为了探究该区域不同下垫面在＂荒漠-绿洲-城市＂演化过程中对区域自然环境以及气象要素的影响。以典型的哈密绿洲为研究区,利用WRF模式分别耦合2000年、201.年2期土地利用数据,模拟对比分析夏季7月哈密绿洲土地利用变化前后区域自然环境要素的影响,采集的指标为2 m气温、2 m相对湿度、地表温度。研究表明：（1.哈密绿洲土地利用变化对气象要素的综合效应表现为：2 m气温升高1.2℃,相对湿度增加2%;（2）下垫面由绿洲转变为城市,2 m气温增加2.5～3℃,地温增加6～7.5℃,相对湿度减小4%～8%;（3）由荒漠变为城市,2 m气温增加1.2℃,地温增加3～6℃,相对湿度减小0～4%;（4）由荒漠变为绿洲,2 m气温降低1.5～4℃,地温降低0～7.5℃,相对湿度增加4%～1.%。总体来看：在中国干旱区,城市化过程使该区域进一步变热和变干,与之相反,绿洲扩张使得区域具有变冷和变湿的趋势。

关键词： WRF 土地利用变化 2m气温 2m相对湿度地表温度哈密绿洲

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拮抗小麦赤霉病内生细菌YB-1.4的鉴定及其对DON的影响

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华北农学报 2020年第6期35卷 172-179页

作者：张立勇夏明聪徐文王琦张洁孙润红潘娅梅陈瑞雪吴坤杨丽荣河南农业大学生命科学学院河南郑州450002 河南省农业科学院植物保护研究所河南省生物农药工程研究中心河南郑州450002 中国农业大学植物保护学院北京100193

为研究控制禾谷镰孢菌生长及抑制其脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)产生的安全、有效生物防治技术,利用植物内生菌分离技术从小麦发病麦穗中分离得到的256株内生菌,通过平板对峙、共培试验,筛选到1.可显著抑制小麦赤霉病菌生... 详细信息

为研究控制禾谷镰孢菌生长及抑制其脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)产生的安全、有效生物防治技术,利用植物内生菌分离技术从小麦发病麦穗中分离得到的256株内生菌,通过平板对峙、共培试验,筛选到1.可显著抑制小麦赤霉病菌生长及降低DON含量的拮抗内生细菌YB-1.4。通过其形态特征、生化特性和1.S rDNA序列比对,结果表明,菌株YB-1.4为贝莱斯芽孢杆菌,菌株YB-1.4对禾谷镰孢菌PH-1.抑菌带宽度为1..6 mm,共培养1. h后孢子萌发抑制率高达81.67%,DON含量下降66.6%,DON合成基因Tri5、Tri3、Tri8的相对表达量分别表现出5.0,1.6,1.2倍的下调。同时菌株YB-1.4对小麦纹枯病菌、小麦根腐病菌等9种植物病原真菌具有显著的抑制作用,该菌株具有较好的开发利用潜力。

关键词：小麦赤霉病禾谷镰孢菌贝莱斯芽孢杆菌脱氧雪腐镰刀菌烯醇内生细菌

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