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Effects of Waterborne Cu and Cd on Anti-oxidative Response, Lipid Peroxidation and Heavy Metals Accumulation in Abalone Haliotis discus hannai Ino

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Journal of Ocean University of China 2015年第3期14卷 511-521页

作者： LEI Yanju zhang Wenbing XU Wei zhang yanjiao ZHOU Huihui MAI Kangsen The Key Laboratory of Aquaculture Nutrition and Feed of Ministry of Agriculture The Key Laboratory of Mariculture of Ministry of Education Fisheries College Ocean University of China

The aim of this study was to compare the effects of waterbome copper （Cu） and cadmium （Cd） on survival, anti-oxida- tive response, lipid peroxidation and metal accumulation in abalone Haliotis discus hannai. Experimental animals （initial weight： 7.49 g±0.01 g） were exposed to graded concentrations of waterborne Cu （0.02, 0.04, 0.06, 0.08 mg L-1） or Cd （0.025, 0.05, 0.25, 0.5 mgL-1） for 28 days, respectively. Activities of the anti-oxidative enzymes （catalase, CAT; superoxide dismutase, SOD; glutathione peroxidases, GPx; glutathione S-transferase, GST）, contents of the reduced glutathione （GSH） and malondiadehyde （MDA） in the hepatopancreas, and metal accumulation in hepatopancreas and muscles were analyzed after 0, 1, 3, 6, 10, 15, 21, 28 days of metal exposure, respectively. Results showed that 0.04 mg L-1, 0.06 mg L-1 and 0.08 mgL-1 Cu caused 100% death of abalone on the 21st, 10th and 6th day, respectively. However, no dead abalone was found during the 28-day waterborne Cd exposure at all experimental concentrations. Generally, activities of SOD and GST in hepatopancreas under all Cu concentrations followed a decrease trend as the exposure time prolonged. However, these activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Activities of CAT in all Cu exposure treatments were higher than those in the control. These activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Contents of MDA in hepatopancreas in all Cu treatments significantly increased first and then decreased to the control level. However, the MDA contents in hepatopan- creas were not significantly changed during the 28-day Cd exposure. The metals accumulation in both hepatopancreas and muscles of abalone significantly increased with the increase of waterborne metals concentration and exposure time. These results indicated that H. discus hannai has a positive anti-oxidative defense aga

关键词： abalone copper cadmium anti-oxidation peroxidation toxicity

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盾构渣土作为护壁泥浆原材料配比预测研究

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国防交通工程与技术 2024年第5期22卷 35-40,50,96页

作者：康佳灵张振波翟逸欣张妍娇张梓函石家庄铁道大学安全工程与应急管理学院河北石家庄050043 河北省大型结构健康诊断与控制重点实验室河北石家庄050043

针对渣土利用为护壁泥浆使用最大化配比设计问题,依托苏州地铁8号线盾构工程,选取盾构穿越粉质黏土地层的渣土、膨润土和水作为护壁泥浆的原材料,通过室内试验测试配制的护壁泥浆性能。将测试结果作为数据集,对比分析了多种机器学习算法... 详细信息

针对渣土利用为护壁泥浆使用最大化配比设计问题,依托苏州地铁8号线盾构工程,选取盾构穿越粉质黏土地层的渣土、膨润土和水作为护壁泥浆的原材料,通过室内试验测试配制的护壁泥浆性能。将测试结果作为数据集,对比分析了多种机器学习算法,比选了盾构渣土作为护壁泥浆原材料配比预测方法。结合现有护壁泥浆使用要求,给出了渣土最大化再利用配比。研究结果表明:①AdaBoost集成的决策树算法相比于决策树、随机森林算法,准确率更高,拟合优度均大于0.8,可作为配比设计的预测方法;②水对比重影响最大,渣土对含砂率影响最大,膨润土对黏度、失水量与泥饼厚度影响最大。在盾构渣土再利用领域,为采用机器学习作为护壁泥浆原材料配比设计的方法进行了有益的探索与尝试。

关键词：盾构渣土护壁泥浆渣土再利用机器学习配比设计

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Effects of Dietary Corn Gluten Meal on Growth Performance and Protein Metabolism in Relation to IGF-I and TOR Gene Expression of Juvenile Cobia (Rachycentron canadum)

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Journal of Ocean University of China 2013年第3期12卷 418-426页

作者： LUO Yiwen AI Qinghui MAI Kangsen zhang Wenbing XU Wei zhang yanjiao LIUFU Zhiguo Key Laboratory of Animal Nutrition and Feed Science Ministry of Agriculture of ChinaOcean University of China

A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and protein metabolism in relation to specific gene *** isonitrogenous(crude protein 45%) and isoenergetic(gross energy 20 kJ g 1) practical diets were formulated by replacing 0%(the control),17.5%,35.0%,52.5%,and 70.0% of fish meal(FM) protein with CGM *** significant differences were observed in the survival,feed intake(FI),specific growth rate(SGR),feed efficiency(FE) and protein productive value(PPV) among fish fed diets with 0%,17.5%,35.0%,and 52.5% of CGM ***,these indices were significantly lower in fish fed the diet with 70.0% of CGM protein than those in fish fed the control diet(P < 0.05).The whole-body crude protein and lipid contents were significantly lower while the whole-body moisture content was significantly higher in fish fed the diet with 70.0% of CGM protein compared with the control group(P < 0.05).When 70.0% of FM protein was replaced by CGM,plasma total protein and cholesterol contents were significantly lower than those in the control group(P < 0.05).Fish fed the diet with 70.0% of CGM protein had significantly lower hepatic insulin-like growth factor I(IGF-I) expression levels than those in the control group(P < 0.05).However,no significant differences were observed in hepatic target of rapamycin(TOR),dorsal muscle IGF-I and TOR expression levels among dietary *** of the present study indicated that 52.5% of FM protein could be replaced by CGM in the diets without significant influences on the growth,feed utilization and protein metabolism of juvenile *** present results might be useful for developing cost effective and sustainable cobia dietary formulations.

关键词： Cobia corn gluten meal growth performance protein metabolism gene expression

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TAT Improves in vitro Transportation of Fortilin Through Midgut and into Hemocytes of White Shrimp Litopenaeus vannamei

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Journal of Ocean University of China 2012年第2期11卷 197-204页

作者： ZHOU Yi zhang Wenbing MAI Kangsen XU Wei zhang yanjiao AI Qinghui WANG Xiaojie The Key Laboratory of Mariculture(Education Ministry of China) Ocean University of ChinaQingdao 266003P.R.China

Fortilin is a multifunctional protein implicated in many important cellular processes. Since injection of Pm-fortilin reduces shrimp mortality caused by white spot syndrome virus (WSSV), there is potential application of fortilin in shrimp culture. In the present study, in order to improve trans-membrane transportation efficiency, the protein transduction domain of the transactivator of transcription (TAT) peptide was fused to fortilin. The Pichia pastoris yeast expression system, which is widely accepted in animal feeds, was used for production of recombinant fusion protein. Green fluorescence protein (GFP) was selected as a reporter because of its intrinsic visible fluorescence. The fortilin, TAT and GFP fusion protein were constructed. Their trans-membrane transportation efficiency and effects on immune response of shrimp were analyzed in vitro. Results showed that TAT peptide improved in vitro uptake of fortilin into the hemocytes and midgut of Litopenaeus vannamei. The phenoloxidase (PO) activity of hemocytes incubated with GFP-Fortilin or GFP-Fortilin-TAT was significantly increased compared with that in the control without expressed fortilin. The PO activity of hemocytes incubated with 200 μg mL-1 GFP-Fortilin-TAT was significantly higher than that in the group with the same concentration of GFP-Fortilin. Hemocytes incubated with GFP-Fortilin-TAT at all concentrations showed significantly higher nitric oxide synthase (NOS) activity than those in the control or in the GFP-Fortilin treatment. The present in vitro study indicated that TAT fusion protein improved the immune effect of fortilin.

关键词： shrimp Litopenaeus vannamei TAT fortilin immunity

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Rearing in intermediate salinity enhances immunity and disease-resistance of turbot (Scophthalmus maximus L.)

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Acta Oceanologica Sinica 2011年第4期30卷 122-128页

作者： zhang yanjiao MAI Kangsen MA Hongming AI Qinghui zhang Wenbing XU Wei Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

Studies were conducted to investigate the non-specific immune responses and disease-resistance of juvenile turbot Scophthalmus maximus,cultured at four different salinities（8,20,32 and 40） . Three concentrations（3.75 × 10 7,3.75 × 10 8 and 3.75 × 10 9 CFU/ml） of Vibrio anguillarum suspension were employed at each salinity to determine the 4-day LD 50 . The serum lysozyme activity,the alternative complement pathway activity（ACH50） and the phagocytosis percentage of head kidney in turbot were tested at 24,48 and 72 h post-challenge of V. anguillarum（1.1 × 10 8 CFU/ml,0.1 ml） ,respectively,to evaluate the non-specific immune responses at the selected rearing salinities. Fish reared at salinity 20 had the lowest mortality,namely,the highest 4-day LD 50 value（8.88 ± 0.17） . Besides,the lysozyme activity,ACH50 and the phagocytosis of turbot were the highest at the salinity 20,but with the lowest at the salinity 40 treatment regardless of sampling time. In addition,the non-specific immune activities kept increasing within 72 h post-challenge of V. anguillarum,except that the lysozyme activity increased from 24 to 48 h,and then decreased from 48 to 72 h at 40 significantly. These results together indicate that rearing in intermediate salinity（20） was able to enhance the immunity and disease-resistance of turbot.

关键词： Scophthalmus maximus salinity immunity disease-resistance Vibrio anguillarum

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盾构渣土作为护壁泥浆最大化再利用研究

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低温建筑技术 2024年第6期46卷 35-38页

作者：翟逸欣张振波康佳灵张妍娇张梓函石家庄铁道大学安全工程与应急管理学院石家庄050043 河北省大型结构健康诊断与控制重点实验室石家庄050043

为实现盾构渣土最大化再利用,文中依托苏州盾构隧道工程,设计了24组不同配比的护壁泥浆试验,基于最小二乘法原理,建立了泥浆性能与原材料配比函数关系,结合规范得到了护壁泥浆渣土含量最大的配比方案。研究表明原材料与护壁泥浆各项指... 详细信息

为实现盾构渣土最大化再利用,文中依托苏州盾构隧道工程,设计了24组不同配比的护壁泥浆试验,基于最小二乘法原理,建立了泥浆性能与原材料配比函数关系,结合规范得到了护壁泥浆渣土含量最大的配比方案。研究表明原材料与护壁泥浆各项指标正负相关性及相关程度,最优配比为渣土:水:膨润土=0.36:0.51:0.125。研究为苏州地区的粉质渣土作为护壁泥浆再利用提供理论依据。

关键词：盾构渣土护壁泥浆粉质黏性渣土

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Effects of Dietary Stachyose on Growth Performance, Digestive Enzyme Activities and Intestinal Morphology of Juvenile Turbot(Scophthalmus maximus L)

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Journal of Ocean University of China 2015年第5期14卷 905-912页

作者： HU Haibin zhang yanjiao MAI Kangsen AI Qinghui XU Wei zhang Wenbing LI Yanxian LIU Jintao Key Laboratory of Aquaculture Nutrition and Feed of Ministry of Agriculture Key Laboratory of Mariculture of Ministry of Education Ocean University of China

A 12-week feeding trial was conducted to evaluate the effects of dietary stachyose on the growth performance, digestive enzymes activities and intestinal structures of juvenile turbot(Scophthalmus maximus L). Five isonitrogenous(49.58% crude protein) and isolipidic(10.50% crude lipid) diets were formulated to contain 0(Control), 0.625%(S-0.625), 1.25%(S-1.25), 2.5%(S-2.5) and 5%(S-5) stachyose, respectively. With the increase of stachyose level, the growth performance and feed utilization of turbot, such as the specific growth rate, final mean body weight, weight gain rate and feed efficiency, increased significantly(P < 0.05) and then stabilized. The feed intake of fish fed S-5 was significantly higher(P < 0.05) than that of fish in other groups. The activities of trypsin, intestinal caseinolytic, stomach and intestinal amylase were significantly influenced by stachyose(P < 0.05). The highest values of trypsin and intestinal caseinolytic activities were observed in group S-1.25, while the highest activity of stomach amylase and the lowest activity of intestine amylase were observed in group S-5. No lesion or damage was found on the distal intestine structures of fish from all treatments, while the height of simple folds in the distal intestine was significantly increased(P< 0.05) when 1.25% or 2.5% stachyose was added in the diets. These results indicated that moderate level of stachyose(1.25%) improves the growth performance, feed utilization, digestive enzyme activities and the distal intestine structures of juvenile turbot.

关键词： stachyose growth digestive enzyme intestinal morphology turbot.

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Proline with or without Hydroxyproline Influences Collagen Concentration and Regulates Prolyl 4-Hydroxylase α(I) Gene Expression in Juvenile Turbot(Scophthalmus maximus L.)

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Journal of Ocean University of China 2015年第3期14卷 541-548页

作者： zhang Kaikai MAI Kangsen XU Wei ZHOU Huihui LIUFU Zhiguo zhang yanjiao PENG Mo AI Qinghui The Key Laboratory of Mariculture (Ministry of Education of China) Ocean University of China

This study was conducted to investigate the effect of dietary proline(Pro), and Pro and hydroxyproline(Hyp) in combination on the growth performance, total Hyp and collagen concentrations of tissues, and prolyl 4-hydroxylase α(I)(P4H α(I)) gene expression in juvenile turbot feeding high plant protein diets. A diet containing 50% crude protein and 12% crude lipid was formulated as the basal and control, on which other two protein and lipid contents identical experimental diets were formulated by supplementing the basal with either 0.75% Pro(Pro-0.75) or 0.75% Pro and 0.75% Hyp(Pro+Hyp). Four groups of fish in indoor seawater recirculating systems, 35 individuals each, were fed twice a day to apparent satiation for 10 weeks. The results showed that dietary Pro and Hyp supplementation had no significant effect on growth performance and feed utilization of juvenile turbot(P > 0.05). Total Hyp and collagen concentrations in muscle were significantly increased when dietary Pro and Hyp increased(P < 0.05), and fish fed diet Pro+Hyp showed significantly higher free Hyp content in plasma than those fed other diets(P < 0.05). The expression of P4 H α(I) gene in liver and muscle was significantly up regulated in fish fed diet Pro-0.75 in comparison with control(P < 0.05); however the gene was significantly down regulated in fish fed diet Pro+Hyp in muscle in comparison with fish fed diet Pro-0.75(P < 0.05). It can be concluded that supplement of crystal L-Pro and L-Hyp to high plant protein diets did not show positive effects on growth performance of juvenile turbot, but enhanced total collagen concentrations in muscle.

关键词： proline hydroxyproline juvenile turbot high plant protein collagen prolyl 4-hydroxylase α(I)

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重构本草——肉苁蓉

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长春中医药大学学报 2024年第4期40卷 371-373页

作者：马凯乐赵旌屹张艳娇田传玺张伟李敏中国中医科学院广安门医院北京100053 甘肃中医药大学基础医学院兰州730000

通过古籍文献调研、中医药现代研究成果的梳理,结合中医临床及中药学等学科领域内专家学者的认识及应用经验,归纳出肉苁蓉:主要功效为补肾阳,益精血,润肠通便。症靶为便秘、阳痿。现代药理研究发现,肉苁蓉及其有效成分具有调节肠道菌群... 详细信息

通过古籍文献调研、中医药现代研究成果的梳理,结合中医临床及中药学等学科领域内专家学者的认识及应用经验,归纳出肉苁蓉:主要功效为补肾阳,益精血,润肠通便。症靶为便秘、阳痿。现代药理研究发现,肉苁蓉及其有效成分具有调节肠道菌群、促进排便、改善生殖功能、促进雄激素分泌、抗氧化、抗衰老、抗骨质疏松、降血糖血脂、抗炎等作用。现有实验数据显示,肉苁蓉是无毒或低毒性物质,阴虚火旺及大便溏泄者不宜服用,肠胃实热、热结便秘者忌用。临床使用剂量为6~60 g,内服时当根据不同疾病,或同一疾病的不同阶段进行辨证使用,并积极探索肉苁蓉用量与症靶的量效关系构建。

关键词：肉苁蓉症靶重构本草临床回归

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Effect of Dietary Lipid on the Growth, Fatty Acid Composition and Δ5 Fads Expression of Abalone(Haliotis discus hannai Ino) Hepatopancreas

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Journal of Ocean University of China 2015年第2期14卷 317-324页

作者： LI Mingzhu MAI Kangsen AI Qinghui HE Gen XU Wei zhang Wenbing zhang yanjiao ZHOU Huihui LIUFU Zhiguo The Key Laboratory of Aquaculture Nutrition and Feed of Ministry of Agriculture The Key Laboratory of Mariculture of Ministry of Education Ocean University of China College of Agriculture Ludong University

This study investigated the effect of dietary lipid on the growth, fatty acid composition and Δ5 fatty acyl desaturase genes(Fads) expression of juvenile abalone(Haliotis discus hannai Ino) hepatopancreas. Six purified diets were formulated to contain tripalmitin(TP), olive oil(OO, 72.87% 18:1n-9), grape seed oil(GO, 68.67% 18:2n-6), linseed oil(LO, 70.48% 18:3n-3), ARA oil(AO, 41.81% ARA) or EPA oil(EO, 44.09% EPA and 23.67% DAH). No significant difference in survival rate was observed among abalone fed with different diets. Weight gain rate(WGR) and daily growth rate of shell length(DGRSL) were significantly increased in abalone fed with diets containing OO, AO and EO, but decreased in abalone fed with LO diet(P < 0.05) in comparison with those fed with TP. High level of dietary 18:2n-6 resulted in higher content of n-6 polyunsaturated fatty acids(PUFAs) in abalone fed with GO than those fed with TP, OO, LO and EO(P < 0.05). n-3 PUFAs in abalone fed with LO was significantly higher than those in abalone fed with TP, OO, GO and AO(P < 0.05). The highest contents of 20:1n-9 and 22:1n-9 were observed in abalone fed with OO. The expression of Δ5 Fads in hepatopancreas of abalone was enhanced by high concentration of 18:3n-3 and suppressed by dietary LC-PUFAs; however it was not affected by dietary high concentration of 18:1n-9 or 18:2n-6. These results provided valuable information for understanding the synthesis of LC-PUFAs and nutritional regulation of Δ5 Fads expression in abalone.

关键词： desaturase fatty acid gene expression, fish oil vegetable oil

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