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子宫NTRK重排的梭形细胞肉瘤2例临床病理学分析

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中华病理学杂志 2024年第5期53卷 480-482页

作者：胡桂明陈慧萍张瑞珍吴会芳郑州大学第二附属医院病理科郑州450014 郑州市妇幼保健院病理科郑州450052

目的探讨子宫NTRK重排的梭形细胞肉瘤的临床病理学特征。方法回顾性分析2例原发于子宫的NTRK重排的梭形细胞肉瘤,包括肿瘤形态学、免疫表型、遗传学特征及预后,并复习相关文献。结果患者年龄分别为29岁(例1)和51岁(例2);肿瘤均位于宫颈... 详细信息

目的探讨子宫NTRK重排的梭形细胞肉瘤的临床病理学特征。方法回顾性分析2例原发于子宫的NTRK重排的梭形细胞肉瘤,包括肿瘤形态学、免疫表型、遗传学特征及预后,并复习相关文献。结果患者年龄分别为29岁(例1)和51岁(例2);肿瘤均位于宫颈,直径分别为26 mm和39 mm,国际妇产科联盟分期均为ⅠA期。低倍镜下密集的梭形肿瘤细胞呈条束状、"人"字形或无序分布,浸润性生长,可见血管外皮瘤样结构;高倍镜下瘤细胞中等大小,轻-中度异型,胞界不清,细胞核卵圆形,染色质粗糙,核仁不明显,核分裂象>20个/10 HPF。免疫组织化学染色显示2例均弥漫表达广谱原肌球蛋白受体激酶(pan-Trk)和cyclin D1,部分表达S-100蛋白和CD34,不表达雌激素受体、孕激素受体、平滑肌肌动蛋白及结蛋白,Ki-67阳性指数分别为40%和70%。采用断裂分离探针荧光原位杂交(FISH)检测发现2例均为NTRK3断裂;RNA靶向二代测序检测,例1存在MYH9-NTRK3融合,例2存在SPECC1L-NTRK3融合。分别随访30和6个月,患者均存活,无复发或转移。结论子宫NTRK重排的梭形细胞肉瘤是一种罕见的恶性肿瘤,组织学形态与多种子宫梭形细胞肿瘤有重叠,免疫组织化学抗体pan-Trk、S-100蛋白、CD34及cyclin D1可用于初筛,借助FISH或二代测序证实NTRK重排或融合有助于选择恩曲替尼或拉罗替尼指导靶向治疗。

关键词：梭形细胞肉瘤临床病理学

Fine mapping of locus S-b for F_1 pollen sterility in rice (Oryza sativa L.)

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Chinese Science Bulletin 2006年第6期51卷 675-680页

作者： LI Wentao ZENG ruizhen zhang Zemin DING Xiaohua zhang Guiquan Guangdong Provincial Key Lab of Plant Molecular Breeding SouthChina Agricultural University Guangzhou 510642 China

Hybrid sterility is the main barrier in util-izing the heterosis of subspecies in rice. A knowledge of the underlying molecular mechanism of the hybrid sterility will be useful for overcoming the barrier. In this research, the F1 pollen sterility locus, S-b, was mapped between SSR markers PSM8 and PSM202. To fine map the locus, one F2 mapping population of 3910 plants was developed using the near-isogenic lines of the locus. Ninety-seven recombinants be-tween two markers were selected. Moreover, a series of markers, including two SSR markers, two InDel markers and four CAPS markers, were developed on the region. Linkage analysis showed that marker W4 was co-segregated with locus S-b, while makers A8 and A14 were located on the two sides of the locus with a distance of 0.026 and 0.038 cM, respectively. The markers were then integrated with the se-quences of the clones of the region. Results showed that all the polymorphic markers were anchored on the three end-to-end jointed clones AC093089, AC079021 and AC134931. According to the physical information of the markers, locus S-b was finally de-limited to a region of 27 kb between A8 and A14. Seven ORFs were identified on the region based on the annotation results of RiceGAAS system. These results laid the foundation for further cloning the gene.

关键词：水稻杂种不育性分子标记杂交第一代花粉不育性

Ecdysteroids in the larvae of Opogona sacchari(Bojer),a invasive alien pest

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Chinese Science Bulletin 2007年第2期52卷 205-208页

作者： WANG FangHai zhang GuRen WEN ruizhen HE GuoFeng State Key Laboratory for Biological Control & Institute of Entomology Sun Yat-sen University Guangzhou 510275 China

In order to understand the composition and quantitative variation of ecdysteriods in the larvae of Opogona sacchari (Bojer), a invasive alien pest, we analyzed the larval ecdysteroid composition and titers in this pest. The main component of ecdysteriods in the larvae of O. sacchari is 20-hydroxyecdysone, and also there is a little 26-hydroxyecdysone. The titer of ecdysteriods in the larvae from the 1st instar to the 7th instar was gotten higher gradually compared on ng ecdyster-oid/larva, but no regularity could be found about the titer if compared on ng ecdysteroid/g avoirdupois. There was only one peak of ecdysteroids (0.5475 ng/larva) showed at day 2 during the developmental time of the 6th instar larvae. However, there were two peaks appearing during the developmental time of the 7th instar larvae , one peak (0.29415 ng/larva) at day 3, another (0.214 ng/larva) at day 5.

关键词：外来生物幼虫害虫植物

Study on the dynamic variation of the secondary metabolites in Viscum coloratum using targeted metabolomics

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Chinese Journal of Natural Medicines 2023年第4期21卷 308-320页

作者： zhang ruizhen DUAN Rong WANG Weiqing YU Zhiguo LI Yun ZHAO Yunli Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical UniversityShenyang 110016China Department of Pharmacy the Second Hospital of Dalian Medical UniversityDalian 116023China

Viscum coloratum(Kom.)Nakai is a well-known medicinal ***,the optimal harvest time for *** is *** studies were performed to analyze compound variation during storage and to improve post-harvest quality *** study aimed to comprehensively evaluate the quality of *** in different growth stages,and determine the dynamic variation of ***-performance liquid chromatography tandem mass spectrometry was used to quantify 29 compounds in *** harvested in six growth periods,and the associated biosynthetic pathways were *** accumulation of different types of compounds were analyzed based on their synthesis *** relational analysis was used to evaluate the quality of *** across different *** compound variation during storage was analyzed by a high-temperature high-humidity accelerated *** results showed that the quality of *** was the hightest in March,followed by November,and became the lowest in *** storage,compounds in downstream steps of the biosynthesis pathway were first degraded to produce the upstream compounds and some low-molecular-weight organic acids,leading to an increase followed by a decrease in the content of some compounds,and resulted in a large gap during the degradation time course among different *** to the rapid rate and large degree of degradation,five compounds were tentatively designated as“early warning components”for quality *** report provides reference for better understanding the biosynthesis and degradation of metabolites in *** and lays a theoretical foundation for rational application of *** and better quality control of *** during storage.

关键词： Viscum coloratum Harvesting period Storage Plant metabolomics Quality control

Ecdysteroids in the pupae of Opogona sacchari (Bojer), a new invasive alien pest

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Chinese Science Bulletin 2006年第11期51卷 1324-1327页

作者： WANG Fanghai zhang Guren WEN ruizhen HE Guofeng State Key Laboratory for Biological Control & Institute of Entomology Sun Yat-sen University Guangzhou 510275 China

In order to understand the composition and quantitative variation of ecdysteriods in the pu- pae of Opogona sacchari (Bojer), a new invasive alien pest, we analyzed the pupal ecdysteroid com- position and titers in this pest. The main component of ecdysteriods in the pupae of O. sacchari is 20-hydroxyecdysone. The titre of ecdysteriods in both male pupae and female pupae get higher gradually, and then decreased slowly after reaching a peak (80 ng/pupa). The titre of ecdysteriods was about 30 ng/pupa in the later stage of pupae. The peak of male pupae appeared on the third day, but the peak of female pupae lasted from the second day to the fourth day during pupal developments. The ecdysteroid content was higher in female pupae than in male pupae during specific pupal time (the 1st, 2nd, 4th, 5th, 6th day). We also found that the titre of ec- dysteroids in posterior part of pupae was higher than in anterior part of pupae obviously.

关键词：蜕皮甾体蛹入侵有害物 Opogona sacchari

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山东省儿童百日咳感染与哮喘的相关性

山东大学学报(医学版) 2024年第7期62卷 33-41页

作者：闫金燕杨春李雷吴福玲焦永莉张晓蔚李晶张瑞珍王磊马香山东大学附属儿童医院感染性疾病科济南市儿童呼吸病重点实验室山东大学附属儿童医院山东第一医科大学附属济南妇幼保健院儿科滨州医学院附属医院儿科枣庄市市中区妇幼保健院儿科济南市章丘区人民医院儿科济宁市任城区妇幼保健院儿科德州市齐河县妇幼保健院儿科泰安市妇幼保健院儿科山东省儿童疾病及健康研究中心济南市儿童医院

目的通过多中心、前瞻性调查，评估不同分期、不同类型哮喘儿童百日咳的感染情况，并分析哮喘与百日咳的相关性。方法通过调查问卷收集因咳嗽持续时间≥1周而就诊的哮喘儿童相关信息，并进行百日咳毒素(pertussis toxin, PT)IgG抗体... 详细信息

目的通过多中心、前瞻性调查，评估不同分期、不同类型哮喘儿童百日咳的感染情况，并分析哮喘与百日咳的相关性。方法通过调查问卷收集因咳嗽持续时间≥1周而就诊的哮喘儿童相关信息，并进行百日咳毒素(pertussis toxin, PT)IgG抗体浓度和/或百日咳核酸检测，对结果进行判读。根据年龄、哮喘分期、是否合并百日咳感染等进行分组，分析不同分组间百日咳抗体分布、感染情况及临床特点等。结果 1 049例哮喘患儿中，PT-IgG总阳性率为26.22%,其中≥6岁组显著高于3个月～0.05)。两组间白细胞计数、中性粒细胞计数、淋巴细胞计数、嗜酸性粒细胞计数及IgE水平差异均无统计学意义(P均>0.05)。结论哮喘儿童百日咳检出率高，尤其是哮喘急性发作与百日咳密切相关，临床症状对两者有一定的鉴别意义，实验室检查结果对两种疾病无鉴别意义。建议对咳嗽持续时间>2～4周的哮喘患儿常规筛查百日咳，以更好地鉴别或判断，指导治疗。

关键词：儿童百日咳感染哮喘相关性

Study on the dynamic variation of the secondary metabolites in Viscum coloratum using targeted metabolomics

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Chinese Journal of Natural Medicines 2023年第04期 308-320页

作者： zhang ruizhen DUAN Rong WANG Weiqing YU Zhiguo LI Yun ZHAO Yunli Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical University Department of Pharmacy the Second Hospital of Dalian Medical University

Viscum coloratum(Kom.) Nakai is a well-known medicinal plant. However, the optimal harvest time for V. coloratum is unknown. Few studies were performed to analyze compound variation during storage and to improve post-harvest quality *** study aimed to comprehensively evaluate the quality of V. coloratum in different growth stages, and determine the dynamic variation of metabolites. Ultra-performance liquid chromatography tandem mass spectrometry was used to quantify 29 compounds in *** harvested in six growth periods, and the associated biosynthetic pathways were explored. The accumulation of different types of compounds were analyzed based on their synthesis pathways. Grey relational analysis was used to evaluate the quality of *** across different months. The compound variation during storage was analyzed by a high-temperature high-humidity accelerated test. The results showed that the quality of V. coloratum was the hightest in March, followed by November, and became the lowest in July. During storage, compounds in downstream steps of the biosynthesis pathway were first degraded to produce the upstream compounds and some low-molecular-weight organic acids, leading to an increase followed by a decrease in the content of some compounds, and resulted in a large gap during the degradation time course among different compounds. Due to the rapid rate and large degree of degradation, five compounds were tentatively designated as “early warning components ” for quality control. This report provides reference for better understanding the biosynthesis and degradation of metabolites in V. coloratum and lays a theoretical foundation for rational application of V. coloratum and better quality control of V. coloratum during storage.

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Ecdysteroids in the adults and eggs of Opogona sacchari (Bojer),an invasive alien pest

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Science China(Life Sciences) 2008年第2期51卷 140-144页

作者： WANG FangHai, zhang GuRen, WEN ruizhen & HE GuoFeng State Key Laboratory for Biological Control and Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China State Key Laboratory for Biological Control and Institute of Entomology Sun Yat-sen University Guangzhou China

In order to understand the composition and quantitative variation of ecdysteriods in the adults and eggs of Opogona sacchari (Bojer), an invasive alien pest, we analyzed the ecdysteroid composition and titers in the adult and egg of this pest. On day 4 after eclosion, the titer of ecdysteriods in the male adult was 0.080 ng/adult, much lower than 5.978 ng/adult in the female adult. During the development of ovaries, the titer of ecdysteriods was low on the first two days, and high in the late period, with the peak (10.48 ng/ovary) appearing on day 3. During the development of eggs, the titer of ecdysteriods was about 0.010 ng/egg from day 1 to day 3, and then decreased to 0.006 ng/egg on day 4. In both adults and eggs, three main components of ecdysteriods were found by identification of HPLC/RIA. They were 20-hydroxyecdysone, 26-hydroxyecdysone, and an unidentified component.

关键词： Opogona sacchari ecdysteroids adults eggs

施用有机肥对土壤理化性状及高丹草产量的影响

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草学 2024年第4期 57-60页

作者：李博杨春桃严东海唐玮琦张瑞珍朱永群程明军李洪泉四川省农业科学院农业资源与环境研究所四川成都610066 四川省草业技术研究推广中心四川成都610041

为减少化肥施用量,探寻有机肥对土壤的改良作用和对高丹草产量的影响,以化肥、普通有机肥、有机无机混合肥为材料,开展高丹草的肥效试验。结果表明,综合配施有机肥或有机无机混合肥可提高土壤有机质、全氮、有效磷、速效钾和碱解氮含量... 详细信息

为减少化肥施用量,探寻有机肥对土壤的改良作用和对高丹草产量的影响,以化肥、普通有机肥、有机无机混合肥为材料,开展高丹草的肥效试验。结果表明,综合配施有机肥或有机无机混合肥可提高土壤有机质、全氮、有效磷、速效钾和碱解氮含量,改良土壤理化环境,提高土壤肥力并增加高丹草产量,适合推广有机无机混合肥施肥模式。

关键词：有机肥土壤理化性状高丹草产量

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