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Chinese Science Bulletin 2008年第9期53卷 1337-1342页

作者： LUO ShiGhun zhang ZhengXi yang li School of Chemistry and Chemical Technology Shanghai Jiao Tong University Shanghai 200240 China

A new asymmetric sulfonium-based ionic liquid, 1-butyldimethylsulfonium bis(trifluoromethylsulfonyl) imide (S114TFSI), was developed as electrolyte material for lithium secondary battery. Its cathodic po- tential was a little more positive against the li/li+, so vinylene carbonate (VC) was added into the liTFSI/S114TFSI ionic liquid electrolyte to ensure the formation of a solid electrolyte interface (SEI), which effectively prevented the decomposition of the electrolyte. The properties of the li/liMn2O4 cell containing S114TFSI-based electrolyte were studied and the cycle performances were compared to those with a conventional organic electrolyte (1 mol/L liPF6/DMC:EC=1:1(w/w)) at room temperature. Electrochemical impedance spectroscopy (EIS) and X-ray diffraction (XRD) were conducted to analyze the mechanisms affecting the cell performances at different temperatures. The lithium secondary bat- tery system, using the above ionic liquid electrolyte material, shows good cycle performances and good safety at room temperature, and is worthwhile to further investigate so as to find out the potential application.

关键词：离子液体锂电池循环利用安全性

Study of the interaction of Zn^(2+) with Aβ(10-21) by fluorescamine assay

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Chinese Chemical Letters 2007年第1期18卷 97-98页

作者： Chao Feng zhang li Fan Pin yang Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Zinc may play a role as a co-factor in the pathogenesis of Alzheimer＇s disease （AD） through influencing the conformation and neurotoxicity of amyloid β-protein （Aβ）. Using the fluorescamine assay, we show for the first time that Zn^2＋ induced Aβ（10-21） aggregate in a concentration-dependent manner. These results indicate that Aβ（10-21） can be used as an in vitro model in Zn^2＋ - induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ（1-42）.

关键词： Aβ(10-21) Fluorescamine Zn^2+ Aggregation

Crystal Structure, Thermal Decomposition Behaviors and Sensitivity Properties of a Novel Energetic Compound [Co（DAT）6] （CIO4）2

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Chinese Journal of Chemistry 2011年第1期29卷 59-64页

作者： Qi, Shuyuan li, Zhimin Zhou, Zunning Cui, Yan zhang, Guotao zhang, Tonglai zhang, Jianguo yang, li State Key Laboratory of Explosion Science and Technology Beijing Institute of Techonoly Beijing 100081 China

A novel energetic coordination compound [Co（DAT）6]（CIO4）2 has been synthesized by using 1,5-diaminotetrazole （DAT） as a ligand and its structure has been characterized using X-ray single crystal diffraction, elemental analysis and FT-IR spectroscopy. The central cobalt（II） cation is coordinated by six N atoms from six DAT molecules to form a six-coordinated and distorted octahedral structure. Di-dimension layer structure was formed by the extensive intermolecular hydrogen bonds between DAT ligands and CIO4 anions along a-axis and b-axis. Thermal decomposition mechanism of [Co（DAT）6]（CIO4）2 was investigated based on differential scanning calorimetry （DSC）, thermogravimetric analysis （TGA） and Fourier transform infrared （FF-IR） spectra. The kinetic parameters of the first exothermic process were studied by applying the Kissinger＇s and Ozawa-Doyle＇s methods. Additionally, the sensitivities of this complex were tested. The results of all the studies show that [Co（DAT）6]（CIO4）2 has an extreme potential application as an energetic material.

关键词： cobalt crystal structure 1,5-diaminotetrazole thermochemistry sensitivity properties

li/liFePO_4 battery performance with a guanidinium-based ionic liquid as the electrolyte

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Chinese Science Bulletin 2011年第27期56卷 2906-2910页

作者： zhang XinYue FANG ShaoHua zhang ZhengXi yang li School of Chemistry and Chemical Technology Shanghai Jiaotong University Shanghai 200240 China

A new guanidinium-based ionic liquid (IL) was investigated as a novel electrolyte for a lithium rechargeable battery. The viscosity, conductivity, lithium redox behavior, and charge-discharge characteristics of the lithium rechargeable batteries were investigated for the IL electrolyte with 0.3 mol kg 1 lithium bis(trifluoromethanesulfonyl)imide (liTFSI) salt. li/ liFePO 4 cells incorporating the IL electrolyte without additives showed good cycle properties at a charge-discharge current rate of 0.1 C, and exhibited good rate capabilities in the presence of a mass fraction of 10% vinylene carbonate or gamma-butyrolactone.

关键词：离子液体电解液电池性能锂离子充电电池胍基氧化还原行为碳酸亚乙烯酯充放电特性

TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance

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Agricultural Sciences in China 2011年第11期10卷 1732-1738页

作者： zhang li-rong yang Wen-xiang liU Da-qun College of Plant Protection Biological Control Center of Plant Diseases and Pests of Hebei Province Agricultural University of HebeiBaoding 071001 P.R.China

Virus-induced gene silencing （VIGS） offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus （BSMV） VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RT- PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.

关键词： disease resistance barley stripe mosaic virus (BSMV) virus-induced gene silencing (VIGS) Lr24 RAR1

Relative bioavailability of tribasic zinc sulfate for broilers fed a conventional corn-soybean meal diet

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Journal of Integrative Agriculture 2015年第10期14卷 2042-2049页

作者： li Wen-xiang MA Xin-yan LU lin zhang li-yang LUO Xu-gang Mineral Nutrition Research Division Institute of Animal Sciences Chinese Academy of Agricultural Sciences Institute of Animal Science Guangdong Academy of Agricultural Sciences

An experiment was carried out to investigate the relative bioavailability of tribasic zinc（Zn） sulfate relative to Zn sulfate for broilers fed a conventional corn-soybean meal diet. A total of 504 1-d-old Arbor Acres commercial male chicks were randomly assigned by body weight of birds to one of seven treatments involving a 2×3 factorial arrangement with three levels of added Zn（30, 60, or 90 mg of Zn kg–1） and two Zn sources（tribasic Zn sulfate and Zn sulfate） plus a control with no added Zn for an experimental phase of 14 d. Plasma Zn, tibia ash Zn, pancreas Zn, and pancreas metallothionein（MT） messenger RNA（m RNA） were analyzed at 6 or 14 d of age post-hatching. The results showed that plasma Zn, tibia ash Zn, pancreas Zn, and pancreas MT m RNA increased linearly（P〈0.002） as dietary Zn concentration increased at 6 or 14 d of age. The R2 for a linear model was greater on d 6 than on d 14 for the above 4 responsive criteria, and among these indices, the fitting of the tibia ash Zn concentration was the best（R2=0.99）. Therefore, based on slope ratios from the multiple linear regressions of the above 4 indices of the birds at 6 d of age on daily intake of dietary Zn, the bioavailabilities of tribasic Zn sulfate relative to Zn sulfate（100%） were 95.6%（P=0.18）, 83.5%（P=0.01）, 87.9%（P=0.53）, and 75.9%（P=0.38） for the tibia ash Zn, pancreas Zn, plasma Zn, and pancreas MT m RNA, respectively. The results indicated that generally, Zn from tribasic Zn sulfate was as available as Zn from Zn sulfate for broilers.

关键词： tribasic zinc sulfate bioavailability Zn concentration MT gene expression broilers

The miRNA let-7al inhibits the expression of insulin-like growth factor 1 receptor （IGFIR） in prostate cancer PC-3 cells

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Asian Journal of Andrology 2013年第6期15卷 753-758,I0006页

作者： li-Na Wang Wei-Wen Chen Ju zhang Chao-yang li Chun-Yan liu Iing Xue Peng-Ju zhang An-li Jiang Institution of Biochemistry and Molecular Biology Medical School of Shandong University Jinan 250012 China

Reduced microRNA （miRNA） let-7a expression and the activation of insulin-like growth factor-1 receptor （IGFIR） signalling are both involved in prostate cancer and progression. In the present study, we demonstrated that the growth inhibitory effect of let-7al is directly related to targeting IGFIRgene expression in PC-3 cells. TargetScan predicted three potential target sites （T1, T2 and T3） of let-7a in the 3＇ untranslational region （3＇ UTR） of IGFIR mRNA. Real-time PCR, Western blot and luciferase reporter assays were used to detect the effects of let-7al overexpression or let-7al inhibitor on the IGFIRgene expression in PC-3 cells. The results indicated that let-7al could inhibit IGFIR expression by directly targeting the T1 and T2 sites in the 3＇ UTR of the IGFIR mRNA. We then used RT-PCR, luciferase reporter assays, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-2H-tetrazolium bromide （MTT） assay, flow cytometry and Hoechst 33342 staining to examine whether let-7al-mediated inhibition of IGFIR expression also affects the IGFIR-mediated signalling events, including Elk1 activity and c-fos gene expression, proliferation, apoptosis and cell cycle. We demonstrated that let-7al-mediated IGFIR downregulation was accompanied by attenuation of Elk1 activity and c-fos expression, inhibition of cell proliferation, enhanced apoptosis and cell cycle arrest, and that loss function of let-7al via inhibition can upregulate IGF1R accompanied by an increase of Elk1 activity and c-fos expression, thereby enhancing cell proliferation. Altogether, these findings sueeest that let-7a mav be novel therapeutic candidate for Drostate cancer.

关键词： IGFIR microRNA (miRNA) prostate cancer

Effect of dietary calcium or phosphorus deficiency on bone development and related calcium or phosphorus metabolic utilization parameters of broilers from 22 to 42 days of age

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Journal of Integrative Agriculture 2020年第11期19卷 2775-2783页

作者： yang Yun-feng XING Guan-zhong li Su-fen SHAO Yu-xin zhang li-yang LU lin LUO Xugang liAO Xiu-dong Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225000P.R.China College of Animal Science and Technology Hebei Normal University of Science and TechnologyQinhuangdao 066004P.R.China

This experiment was conducted to investigate the effect of dietary calcium(Ca)or phosphorus(P)deficiency on bone development and related Ca or P metabolic utilization parameters of broilers from 22 to 42 days of age based on our previous study,which indicated that dietary Ca or P deficiency impaired the bone development by regulating related Ca or P metabolic utilization parameters of broilers from 1 to 21 days of age.A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates in a completely randomized design,and fed the normal control and Ca-or P-deficient diets from 1 to 21 days of *** 22 days of age,the broilers were further fed the normal control diet(0.90%Ca+0.35%non-phytate P(NPP)),the P-deficient diet(0.90%Ca+0.18%NPP),the Ca-deficient diet(0.30%Ca+0.35%NPP)or the Ca and P-deficient diet(0.30%Ca+0.18%NPP),*** results showed that dietary Ca or P deficiency decreased(Pline phosphatase(ALP)activity of broilers on day 42 compared with the control ***,the broilers fed the P-deficient diet had the lowest(Plic utilization parameters of broilers were the most sensitive to dietary P deficiency,followed by dietary Ca deficiency or Ca and P-deficiency;dietary Ca or P deficiency impaired the bone development possibly by regulating serum Ca and P contents as well as tibia Ca content and ALP activity of broilers from 22 to 42 days of age.

关键词： calcium deficiency phosphorus deficiency bone development metabolic utilization parameter broiler

Effect of dietary supplementation with flavonoid from Scutellaria baicalensis Georgi on growth performance,meat quality and antioxidative ability of broilers

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Journal of Integrative Agriculture 2018年第5期17卷 1165-1170页

作者： liAO Xiu-dong WEN Qian zhang ling-yan LU lin zhang li-yang LUO Xu-gang Mineral Nutrition Research Division Institute of Animal ScienceChinese Academy of Agricultural Sciences

An experiment was conducted to investigate the effect of dietary supplementation with flavonoid from Scutellaria baicalensis Georgi（SBGFN） as SBGFN-zinc（SBGFN-Zn） on growth performance, meat quality, immune responses and antioxidation of broilers. A total of 450 one-d-old Arbor Acres male broilers were randomly allocated to 5 treatments with 6 replicates of 15 birds per replicate for each treatment in a completely randomized design. Birds were fed a SBGFN-unsupplemented cornsoybean meal basal diet（control） or the basal diet supplemented with 60, 120, 180 or 240 mg SBGFN kg-（–1） from SBGFN-Zn for 42 d. Dietary SBGFN supplementation affected（P〈0.03） drip loss in thigh muscle, total superoxide dismutase（T-SOD） and glutathione peroxidase（GSH-Px） activity in liver of broilers at 42 d of age. Chicks fed the diets supplemented with 120, 180 and 240 mg SBGFN kg-（–1） had lower（P〈0.03） drip loss of thigh muscle than those fed the control diet. Chicks fed the diet supplemented with 180 mg SBGFN kg-（–1） had higher（P〈0.03） liver T-SOD and GSH-Px activity than those fed the diets supplemented with 0, 60 and 120 mg SBGFN kg-（–1）. The results from the present study indicate that dietary supplementation with 180 mg SBGFN kg-（–1） as SBGFN-Zn improved both meat quality and antioxidative ability of broilers.

关键词： flavonoid growth performance meat quality antioxidation

RESEARCH AND APPliCATION OF A NEURAL NETWORK CLASSIFIER BASED ON DYNAMIC THRESHOLD

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Journal of Electronics(China) 2009年第3期26卷 407-411页

作者： zhang li Luo Jianhua yang Suying School of Electronic and Information Engineering Dalian University of Technology Dalian 116023 China

In this study, a Multi-Layer BP neural network(MLBP) with dynamic thresholds is employed to build a classifier *** to the design of the neural network structure, theoretical guidance and plentiful experiments are combined to optimize the hidden layers' parameters which include the number of hidden layers and their node *** classifier with dynamic thresholds is used to standardize the output for the first time, and it improves the robustness of the model to a high ***, the classifier is applied to forecast box office revenue of a movie before its theatrical *** comparison results with the MLP method show that the MLBP classifier model achieves more satisfactory results, and it is more reliable and effective to solve the problem.

关键词： Neural network classifier Dynamic threshold Forecasting Box office revenue