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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Mei HAN~1 Yan zhang~1 Ru-Yi HUANG~2 Zhi-Qi zhao~1 Yu-Qiu zhang~(1 *) 1 Institutes of Neurobiology Institutes of Brain Science and State Key Laboratory of Medical Neurobiology Fudan university shanghai 200032 china 2 department of Life Science Fudan university shanghai 200433 china

＜正＞Objective SNARE complex,including SNAβ-25,syntaxin and VAMP,plays an important role in presynaptic neurotransmitter ***β25 interacting protein of 30kD（SIP30）that was first identified in the cochlear hair cells of guinea pig organ of Corti is expressed in many organs but is most abundant in ***,its functional significance remains *** evidence demonstrates that the anterior cingulated cortex（ACC） is implicated in the affective response to noxious *** present study is to investigate whether SEP30 in the ACC play roles in pain-related negative affect in rat and its possible *** The localization of SEP30 was detected by *** chronic constriction injury（CCI） of sciatic nerve was used for neuropathic pain *** escape/avoidance paradigm （PEAβ） test was used to reflect the pain-related negative *** neural processing relating learning and memory was tested by conditioned place avoidance（CPA） induced by electric foot-shock or *** behavioral pain hypersensitivities were measured by Von Frey test for mechanical allodynia and Hargreves’test for thermal ***-ACC microinjection of SIP30 small interfering（siRNA） by letivirus as vector was used for knockdown the expression of SIP30. The efficiency of SIP30 knockdown in the ACC was tested by Western Blot and ***（1） SIP30 especially expressed on neuron not astrocyte or microglia in the rACC.（2） In the PEAβtest,the percentage of time spent in the light side of the chamber in the CCI group was much higher than that of sham group,which means PEA has occurred. （3） Compared with sham CCI group,SIP30 significantly upregulated in the rACC after PEAβtest in CCI group.（4） SIP30 knockdown in the rACC had no effect on CCI-induced pain hypersensitivities and innocuous stimuli-induced CPA.（5） Following SIP30 siRNA microinjected into the rACC,place escape/avoidance behavior to a noxious mechanical stimuli was attenua

关键词： SIP30 pain-related negative affect rACC rat

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RNA m^(6)A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progression

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Genes & Diseases 2024年第1期11卷 382-396页

作者： Zheng zhang Keren Zhou Li Han Andrew Small Jianhuang Xue Huilin Huang Hengyou Weng Rui Su Brandon Tan Chao Shen Wei Li Zhicong zhao Ying Qing Xi Qin Kitty wang Keith Leung Mark Boldin Chun-Wei Chen David Ann Zhijian Qian Xiaolan Deng Jianjun Chen Zhenhua Chen department of Systems Biology Beckman Research Institute of City of HopeMonroviaCA 91016USA Irell and Manella Graduate School of Biological Sciences Beckman Research Institute of City of HopeDuarteCA 91010USA Gehr Family Center for Leukemia Research City of HopeDuarteCA 91010USA Sun Yat-sen university Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdong 510060China Guangzhou Laboratory GuangzhouGuangdong 510005China Bioland Laboratory GuangzhouGuangdong 51005China department of Liver Surgery Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China department of Diabetes Complications and Metabolism Beckman Research Institute of City of HopeDuarteCA 91010USA department of Biochemistry and Molecular Biology University of FloridaGainesvilleFL 32603USA tongji Hospital Affiliated to tongji university Frontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China School of Pharmacy China Medical UniversityShenyangLiaoning 110001China

As the most common internal modification of mRNA,Ne-methyladenosine(m^(6)A)and its regulators modulate gene expression and play critical roles in various biological and patholog-ical processes including *** was reported previously that m^(6)A methyltransferase(writer),methyltransferase-like 3(METTL3)adds m^(6)A in primary microRNAs(pri-miRNAs)and fa-cilitates its processing into precursor miRNAs(pre-miRNAs).However,it is unknown whether m^(6)A modification also plays a role in the maturation process of pre-miRNAs and(if so)whether such a function contributes to ***,we found that YTHDF2 is aberrantly overexpressed in acute myeloid leukemia(AML)patients,especially in relapsed patients,and plays an onco-genic role in ***,YTHDF2 promotes expression of miR-126-3p(also known as miR-126,as it is the main product of precursor miR-126(pre-miR-126)),a miRNA that was reported as an oncomiRNA in AML,through facilitating the processing of pre-miR-126 into mature ***,YTHDF2 recognizes m^(6)A modification in pre-miR-126 and recruits AGO2,a regulator of pre-miRNA processing,to promote the maturation of ***2 posi-tively and negatively correlates with miR-126 and miR-126's downstream target genes,respec-tively,in AML patients,and forced expression of miR-126 could largely rescue YTHDF2/Ythdf2 depletion-mediated suppression on AML cell growth/proliferation and leukemogenesis,indi-cating that miR-126 is a functionally important target of YTHDF2 in ***,our studies not only reveal a previously unappreciated YTHDF2/miR-126 axis in AML and highlight the ther-apeutic potential of targeting this axis for AML treatment,but also suggest that m^(6)A plays a role in pre-miRNA processing that contributes to tumorigenesis.

关键词： AML miR-126 N^(6)-methyladenosine Pre-miRNA maturation YTHDF2

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Inhibition of Bcl-2 expression by a novel tumor-specific RNA interference system increases chemosensitivity to 5-fluorouracil in Hela cells

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Acta Pharmacologica Sinica 2006年第2期27卷 242-248页

作者： Sheng-lin HUANG Yi WU Hai YU Ping zhang Xing-qian zhang Lei YING Han-fang zhao Research Center for Human Gene Therapy,department of Biochemistry and Molecular Biology.shanghai Jiao Tong university School of Medicine,shanghai 200025.china department of Biochemistry and Molecular Biology Research Center for Human Gene Therapy Shanghai Jiao Tong University School of Medicine Shanghai China

Aim:RNA interference(RNAi)has been proposed as a potential treatment forcancer,but the lack of cellular targets limits its use in cancer gene *** technology has achieved direct tumor-specific gene silencing using *** the present study we attempt to develop a tumor-specific RNAi system usingthe human telomerase reverse transcriptase(hTERT)promoter:furthermore,weanalyzed its inhibitive effect on Bcl-2 ***:The vectors contain-ing a small hairpin RNA(shRNA)to target exogenous reporters[firefly luciferaseand enhanced green fluorescent protein(EGFP)]and endogenous gene(Bcl-2)were *** expression was determined by dual luciferase *** transcription-polymerase chain reaction(RT-PCR),fluorescence micros-copy and fluorescence-activated cell sorting(FACS)were used to measure EGFPexpression,Inhibition of Bcl-2 was evaluated by RT-PCR and Western *** proliferation and viability were measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)*** was used to analyzethe cell cycle distribution ***:We showed that with the hTERTpromoter directly driving shRNA transcription,expression of the exogenous re-porters(LUC and EGFP)in tumor cells,but not normal cells,was specificallyinhibited in *** hTERT promoter-driven shRNA also depressed the expres-sion of *** of Bcl-2 did not affect cell proliferation,but increased thechemosensitivity of HeLa cells to ***:The present studydescribes an efficient RNAi system for gene silencing that is specific to tumorcells using the hTERT *** of Bcl-2 by using this system sen-sitized HeLa cells to *** system may be useful for RNAi therapy.

关键词： RNA interference telomerase promoter regions (Genetics) Bcl-2 fluorouracil gene therapy

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LiNbO_3:Fe晶体中光写入波导时折射率的变化规律

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物理学报 2003年第5期52卷 1179-1183页

作者：杨德兴赵建林张鹏李碧丽冯锡淇西北工业大学应用物理系光子学研究室西安710072 中国科学院上海硅酸盐研究所无机功能材料重点实验室上海200050

研究了利用光辐照法制作光折变波导时LiNbO3:Fe晶体中折射率变化的规律 .分别采用波长为 63 2 8nm和5 3 2nm的寻常偏振和非常偏振的细激光束和片状激光束 ,在LiNbO3:Fe晶体中进行了写入波导实验 .研究表明 ,制作波导的写入光宜采用寻... 详细信息

研究了利用光辐照法制作光折变波导时LiNbO3:Fe晶体中折射率变化的规律 .分别采用波长为 63 2 8nm和5 3 2nm的寻常偏振和非常偏振的细激光束和片状激光束 ,在LiNbO3:Fe晶体中进行了写入波导实验 .研究表明 ,制作波导的写入光宜采用寻常偏振光 .在利用由光束辐照LiNbO3:Fe晶体形成的正折射率变化区域作为波导结构时 ,必须严格控制辐照时间 .否则 ,由于长时间光辐照会带来较强的噪音栅以及折射率变化区域会发生扩展 ,而难以形成优质波导 .利用片光在“三明治”方式辐照下 ,以小曝光量制作波导时 ,可以避免噪音栅的影响 ,形成高质量的对称型光波导 .通过选择不同的光束宽度和片光间距。

关键词： LiNbO3:Fe晶体铌酸锂晶体铁掺杂折射率变化规律写入波导实验光折变波导光辐照

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High critical current density in Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12) electrolyte via interfacial engineering with complex hydride

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Rare Metals 2024年第2期43卷 692-701页

作者： Ying-Tong Lv Teng-Fei zhang zhao-Tong Hu Guang-Lin Xia Ze-Ya Huang Zhen-Hua Liu Li-Hua Que Cai-Ting Yuan Fang-Qin Guo Takayuki Ichikawa Xue-Bin Yu College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing210016China Centre for Hydrogenergy College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing210016China department of Materials Science Fudan UniversityShanghai200433China Graduate School of Advanced Science and engineering Hiroshima University1-4-1 KagamiyamaHigashi-Hiroshima739-8527Japan

Garnet-type solid-state batteries(SSBs)are considered to be one of the most promising candidates to realize next-generation lithium metal batteries with high energy density and ***,the dendrite-induced short-circuit and the poor interfacial contact impeded the practical ***,interface engineering to achieve low interfacial resistance without high temperature calcination was developed,which Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)was simply coated with complex hydride(Li_(4)(BH_(4))_(3)I(3L1L))in various mass ratios n(Li_(4)(BH_(4))_(3)I)-(100−n)LLZTO(10≤n≤40).The interfacial conductivity increases by more than three orders of magnitude from 8.29×10^(−6)S·cm^(−1)to 1.10×10^(−2)S·cm^(−1).Symmetric Li cells exhibit a high critical current density(CCD)of 4.0 mA·cm^(−2) and an excellent cycling stability for 200 h at 4.0 mA·cm^(−2).SSBs with polymeric sulfur-polyacrylonitrile(SPAN)cathode achieve a high discharge capacity of 1149 mAh·g^(−1) with a capacity retention of 91%after 100 cycles(0.2 C).This attempt guides a simple yet efficient strategy for obtaining a stable Li/LLZTO interface,which would promote the development of solid-state batteries.

关键词： Hydrides Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO) Critical current density Solid-state electrolytes(SSEs) Lithium-sulfur batteries

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非那雄胺2种片剂的药动学和相对生物利用度比较

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中国新药与临床杂志 2004年第10期23卷 684-686页

作者：张国庆柴逸峰张银娣王彬沈建平王慧赵亮中国人民解放军第二军医大学东方肝胆外科医院药材科上海200438 中国人民解放军第二军医大学药学院上海200433 南京医科大学临床药理室江苏南京210029

目的 :比较非那雄胺 2种片剂在健康受试者体内的药动学和相对生物利用度。方法 :2 0名男性健康受试者随机交叉口服非那雄胺受试片与参比片 ,剂量均为 5mg ,采用液相色谱质谱联用技术测定血浆中非那雄胺浓度。结果 :非那雄胺受试片和... 详细信息

目的 :比较非那雄胺 2种片剂在健康受试者体内的药动学和相对生物利用度。方法 :2 0名男性健康受试者随机交叉口服非那雄胺受试片与参比片 ,剂量均为 5mg ,采用液相色谱质谱联用技术测定血浆中非那雄胺浓度。结果 :非那雄胺受试片和参比片的主要药动学参数Cmax为 (5 0±s 11)和 (5 3± 12 ) μg·L- 1,Tmax为 (2 .4± 0 .8)和 (2 .3± 0 .8)h ,T1/2 为 (6 .2± 0 .9)和 (6 .4± 0 .7)h ,AUC0 2 4 为 (317± 91)和 (30 7± 86 ) μg·h·L- 1。非那雄胺受试片对参比片的相对生物利用度为 (10 4± 14 ) %。结论 :双单侧t检验显示

关键词：非那雄胺片剂药动学生物利用度液相色谱法光谱分析质量

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HIGH RESOLUTION OPTICAL SPECTRA OF COMET C/1995 O1(HALE-BOPP)

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中国科学院上海天文台年刊 2000年 55-58页

作者： zhang Huawei 1,2,3 zhao Gang 3 Hu Jingyao 3 ( 1. department of Geophysics, Peking university, Beijing 100871) (2. Beijing Astrophysics Center, Jointly sponsored by the Chinese Academy of Sciences and Peking university, Beijing 100871) (3

High resolution, high signal-to-noise ratio spectra of Comet C/1995 O1 (Hale-Bopp) which provided continuous wavelength coverage from 5500  to 8500  and partial coverage from 4000  to 5500  were obtained with Coudé Echelle Spectrograph at Beijing Astronomical Observ atory on March 26, 28 and April 22, 1997. In the spectra we found 751 emission features among which 577 lines from H, O, Na, C 2, C 3, CN, CH, NH 2, H 2O + were identified.

关键词： Comets - General-Comets - Individual(C/1995 O1)

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Activation of p38/MAPK in the anterior cingulate cortex contributes to pain-related negative emotion in rats

Activation of p38/MAPK in the anterior cingulate cortex cont...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Hong CAO~(1,2),Gen-Cheng WU~2,Zhi-Qi zhao~1,Yu-Qiu zhang~1 1 Institute of Neurobiology 2 department of Integrative Medicine and Neurobiology,Institutes of Brain Research and State Key Laboratory of Medical Neurobiology shanghai Medical College,Fudan university,shanghai 200032,china

＜正＞Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage, including a sensory-discriminative and an emotional-affective *** evidence from morphological, electrophysiological,neuroimaging,and behavioral studies as well as early clinical observation indicates that the anterior cingulate cortex（ACC） is involved in pain-related ***,little is known about the molecular and cellular mechanisms underlying pain-related aversion in *** previous studies showed that NMDAR-dependent ERK signaling cascade in rat ACC contributes to pain *** the present study we observed the role of p38,another member of MAP（mitogen-activated protein） kinase family,in ACC in pain-related negative emotion using Western Blot and behavioral ***-plantar injection of 5%formalin produced a robust increase in phosphorylated MKK3/6（pMKK3/6）,upstream of p38/MAPK,and phosphorylated p38（p-p38） in the bilateral rostral ACC（rACC）.Using formalin-induced conditioned place avoidance（F-CPA） in rats,which is believed to reflect the pain-related negative emotion,we found that blockade of p38/MAPK activation in the rACC with p38 inhibitor SB203580（0.1μg,1μg per side） but not low dose（0.01μg per side）,or another kind of p38 inhibitor SB239063（0.1μg,1μg per side） dose-dependently prevented the induction of ***,these blockades affected neither formalin-induced two-phase spontaneous pain nor low-intensity electric foot shock-induced CPA *** together,these results indicate that activation of p38/MAPK contributes to pain-related negative emotion,which will provide new molecule mechanisms underlying pain affect and bring new treatments for relieving emotional pain-related disturbance.

关键词： p38/MAPK formalin-induced conditioned place avoidance pain-related negative emotion anterior cingulate cortex rat

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New method to identify optimal discontinuity set number of rock tunnel excavation face orientation based on Fisher mixed evaluation

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Underground Space 2024年第4期17卷 300-319页

作者： Keshen zhang Wei Wu Min zhang Yongsheng Liu Yong Huang Baolin Chen College of Civil engineering Tongji UniversityShanghai 200092China Key Laboratory of geotechnical and Underground engineering of Ministry of Education Tongji UniversityShanghai 200092China State Key Laboratory of Disaster Reduction in Civil engineering Tongji UniversityShanghai 200092China Shudao Investment Group Co.Ltd Chengdu 610095China china Railway Tunnel Group Co.Ltd Guangzhou 511466China china Railway First Survey and Design Institute Group Co.Ltd Xi’an 710043China Zhejiang Institute of Communications Co.Ltd Hangzhou 310012China

Discontinuity is critical for strength,deformability,and permeability of rock *** information is one of the essential discontinuity characteristics and is usually accessed by orientation *** methods of identifying optimal discontinuity set numbers are usually achieved by clustering validity indexes,which mainly relies on the aggregation and dispersion of clusters and leads to the inaccuracy and instability of *** paper proposes a new method of Fisher mixed evaluation(FME)to identify optimal group numbers of rock mass discontinuity *** FME,orientation distribution is regarded as the superposition of Fisher mixed *** grouping results are identified by considering the fitting accuracy of Fisher mixed distributions,the probability monopoly and central location significance of independent Fisher centers.A Halley-Expectation-Maximization(EM)algorithm is derived to achieve an automatic fitting of Fisher mixed *** real rock discontinuity models combined with three orientation clustering algorithms are adopted for discontinuity *** clustering validity indexes are used to automatically identify optimal group numbers for *** results show that FME is more accurate and robust than the other clustering validity indexes in optimal discontinuity group number identification for different rock models and orientation clustering algorithms.

关键词： Rock mass discontinuity Orientation grouping Fisher mixed distribution 3D point cloud Stereo photogrammetry

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The 2^(nd)Asian Conference on Porphyrins,Phthalocyanines and Related Materials

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Chinese Chemical Letters 2024年第7期35卷 1-3页

作者： Gaojie Zhu Zhen Yang Shijun Li Weihua Zhu Rui Cao Junlong zhang Jianzhang zhao Jonathan L.Sessler Xunjin Zhu Jianxin Song Yongshu Xie Jianzhuang Jiang Key Laboratory for Advanced Materials and Institute of Fine Chemicals School of Chemistry and Molecular EngineeringEast China University of Science&TechnologyShanghai 200237China College of Material Chemistry and Chemical EngineeringKey Laboratory of Organosilicon Chemistry and Material Technology of Ministry of EducationHangzhou Normal UniversityHangzhou 311121China School of Chemistry and Chemical engineering Jiangsu UniversityZhenjiang 212013China Key Laboratory of Applied Surface and Colloid Chemistry Ministry of EducationSchool of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi'an 710119China Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China State Key Laboratory of Fine Chemicals Frontier Science Center for Smart MaterialsSchool of Chemical EngineeringDalian University of TechnologyDalian 116024China department of Chemistry The University of TexasAustinTexas 78712-1224United States department of Chemistry and Institute of Advanced Materials Hong Kong Baptist UniversityHong Kong 999077China Key Laboratory of Chemical Biology and Traditional Chinese Medicine(Ministry of Educational of china) Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan ProvinceCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China Beijing Advanced Innovation Center for Materials Genome engineering Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline MaterialsDepartment of Chemistry and Chemical EngineeringSchool of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijing 100083China

In the past decades,porphyrins,phthalocyanines and related materials have attracted significant attention due to their diverse and brilliant structures[1,2],as well as their unique electronic structures and photophysi... 详细信息

关键词： cyanine phthalocyanine Porphyrin

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