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Spike timing-dependent plasticity of neocortical excitatory synapses on inhibitory interneurons depends on target cell type

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Spike timing-dependent plasticity of neocortical excitatory ...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Jiang-Teng LU~1,Cheng-Yu LI~1,Jian-Ping zhao~1,Mu-Ming POO~(1,2*),Xiao-Hui zhang~(1*) 1 Institute of Neuroscience and Key Laboratory of Neurobiology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,china 2 Division of Neurobiology,department of Molecular and Cell Biology,Helen Wills Neuroscience Institute,university of California,Berkeley,CA 94720,USA

＜正＞Repetitive correlated spiking can induce long-term potentiation（LTP） and long-term depression（LTD） of many excitatory synapses on glutamatergic neurons,in a manner that depends on the timing of pre- and postsynaptic spiking. However,it is largely unknown whether and how such spike timing-dependent plasticity（STDP） operates at neocortical excitatory synapses on inhibitory interneurons,which have diverse physiological and morphological *** this study we found that these synapses exhibit target cell-dependent *** layer 2/3 of the somatosensory cortex,the pyramical cell（PC） forms divergent synapses on fast spiking（FS） and low-threshold spiking（LTS） interneurons that exhibit short-term synaptic depression and facilitation in response to high-frequency stimulation,*** PC-LTS synapses,repetitive correlated spiking induced LTP or LTD,depending on the timing of pre- and postsynaptic spiking. However,regardless of the timing and frequency of spiking,correlated activity induced only LTD at PC-FS *** target cell-specific STDP was not caused by the difference in the short-term plasticity between these two types of *** of postsynaptic N-methyl-D-aspartate subtype of glutamate receptors（NMDARs） was required for LTP induction at PC-LTS synapses,whereas activation of metabotropic glutamate receptors（mGluRs） was required for LTD induction at both PC-LTS and PC-FS *** absence of LTP at PC-FS synapses may be attributed in part to a much lower level of NMDARs relative to non-NMDARs,as compared to that at PC-LTS *** dependence of STDP on postsynaptic interneuron types may contribute to differential processing and storage of information in cortical local circuits.

关键词： synaptic plasticity spike timing-dependent plasticity(STDP) excitatory synapse inhibitory interneuron target-cell specificity somatosensory cortex

表面麻醉下白内障手术配合欠佳老年患者术中体验的质性研究

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军事护理 2024年第8期41卷 94-96页

作者：张文娟张睿赵敏赵春艳海军军医大学第一附属医院眼科上海200433

目的了解表面麻醉下白内障手术中配合欠佳老年患者的真实感受,为提高该类患者术中配合度及手术安全性提供参考。方法于2022年9月至2023年6月目的抽样法选取某三级甲等医院眼科表面麻醉下行白内障手术术中配合欠佳的17例老年患者作为研... 详细信息

目的了解表面麻醉下白内障手术中配合欠佳老年患者的真实感受,为提高该类患者术中配合度及手术安全性提供参考。方法于2022年9月至2023年6月目的抽样法选取某三级甲等医院眼科表面麻醉下行白内障手术术中配合欠佳的17例老年患者作为研究对象,采用现象学研究法进行半结构化访谈,并使用Colaizzi 7步分析法对数据进行整理和分析。结果归纳出3个主题:对白内障手术相关知识了解不足;负性心理体验(对手术环境的陌生、手术操作引起的负性心理、担心手术预后);术中畏光,固视时间久。结论为了提高老年患者白内障手术的术中配合度,护理人员应完善健康宣教及科普,全方位提高患者对疾病的认知度,并采取有效措施疏导患者情绪,减轻其心理负担,以改善术中配合度,提高手术安全性。

关键词：白内障术中配合心理感受质性研究

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Probabilistic analysis of tunnel face seismic stability in layered rock masses using Polynomial Chaos Kriging metamodel

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Journal of Rock Mechanics and geotechnical engineering 2024年第7期16卷 2678-2693页

作者： Jianhong Man Tingting zhang Hongwei Huang Daniel Dias department of geotechnical engineering Key Laboratory of Geotechnical and Underground Engineering of Ministry of EducationCollege of Civil EngineeringTongji UniversityShanghai200092China Grenoble Alpes 3SR LabCNRS UMR 5521Grenoble38000France

Face stability is an essential issue in tunnel design and *** rock masses are typical and ubiquitous;uncertainties in rock properties always *** view of this,a comprehensive method,which combines the Upper bound Limit analysis of Tunnel face stability,the Polynomial Chaos Kriging,the Monte-Carlo Simulation and Analysis of Covariance method(ULT-PCK-MA),is proposed to investigate the seismic stability of tunnel faces.A two-dimensional analytical model of ULT is developed to evaluate the virtual support force based on the upper bound limit *** efficient probabilistic analysis method PCK-MA based on the adaptive Polynomial Chaos Kriging metamodel is then implemented to investigate the parameter uncertainty *** input parameters,including geological strength indices,uniaxial compressive strengths and constants for three rock formations,and the horizontal seismic coefficients,are treated as random *** effects of these parameter uncertainties on the failure probability and sensitivity indices are *** addition,the effects of weak layer position,the middle layer thickness and quality,the tunnel diameter,the parameters correlation,and the seismic loadings are investigated,*** results show that the layer distributions significantly influence the tunnel face probabilistic stability,particularly when the weak rock is present in the bottom *** efficiency of the proposed ULT-PCK-MA is validated,which is expected to facilitate the engineering design and construction.

关键词： Tunnel face stability Layered rock masses Polynomial Chaos Kriging(PCK) Sensitivity index Seismic loadings

BNIP3-AIF pathway is involved in hippocampal neuronal death induced by oxygen-glucose deprivation

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BNIP3-AIF pathway is involved in hippocampal neuronal death ...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Shen-Ting zhao~1,Ming CHEN~1,Shu-Ji LI~1,Ming-Hai zhang~1,Bo-Xing LI~1,Xin-Hong ZHU~1,Ji-Ming KONG~(1,2),Tian-Ming GAO~(1,*) 1 department of Neurobiology,Southern Medical university,Guangzhou 510515,china 2 department of Human Anatomy and Cell Science,university of Manitoba Faculty of Medicine,Winnipeg,Man., Canada

＜正＞Objective Evidences have shown that mitochondrial protein apoptosis-inducing factor（AIF） is involved in caspase-independent neuronal apoptosis induced by ***,its upstream signaling mechanism remains *** we test the hypothesis that BNIP3 may be an upstream signal of AIF that mediates ischemic neuronal death. Methods We performed immunocytochemistry,Western blotting,cell transfection,subcellular fractionation,and RNA interfering to analyze the expression and localization of BNTP3 and AIF in primary cultured hippocampal neurons subjected to oxygen-glucose deprivation（OGD）.Results After OGD,both BNIP3 and AIF are *** translocates from mitochondria to nucleus in a time-dependent manner which follows the mitochondrial BNIP3 ***,the translocation of AIF occurs hours before the observable nuclear *** of BNIP3 induces nuclear translocation of AIF and the neuronal death whereas inhibition of BNIP3 decreases OGD-induced AIF translocation and neuronal ***3-induced neuronal death is also blocked by reducing AIF *** BNIP3-AIF pathway is involved in OGD-induced hippocampal neuronal death.

关键词： oxygen-glucose deprivation hippocampal neuron apoptosis BNIP3 AIF

CBX7在胃癌干细胞样细胞中的表达及其功能研究

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中国癌症杂志 2013年第2期23卷 114-119页

作者：赵丽琴郭伟剑张晓伟屈晓飞王小凤倪苏婕黄明主复旦大学附属肿瘤医院肿瘤内科复旦大学上海医学院肿瘤学系上海200032 华中科技大学同济医学院附属协和医院肿瘤中心湖北武汉430022

背景与目的:色素框同源蛋白7(chromobox protein homolog 7,CBX7)是一种参与调控细胞增殖衰老的转录抑制因子,属于PcG家族成员,其在维持胚胎干细胞自我更新和全能性中发挥重要作用,但CBX7在肿瘤干细胞中的表达和作用鲜见报道。本研究旨... 详细信息

背景与目的:色素框同源蛋白7(chromobox protein homolog 7,CBX7)是一种参与调控细胞增殖衰老的转录抑制因子,属于PcG家族成员,其在维持胚胎干细胞自我更新和全能性中发挥重要作用,但CBX7在肿瘤干细胞中的表达和作用鲜见报道。本研究旨在探讨CBX7在胃癌干细胞样细胞中的表达及其对胃癌干细胞相关特性的调节作用。方法:采用无血清悬浮培养法从MKN28胃癌细胞系中分离干细胞样亚群,蛋白质印迹法(Western blot)检测干细胞相关因子Oct4、β-catenin和CBX7蛋白表达。脂质体法将CBX7干扰质粒(CBX7i)和对照质粒(Ctrli)分别转染入MKN28胃癌细胞,转染后荧光显微镜观察转染效率,Western blot检测CBX7下调效果,无血清悬浮培养检测细胞自我更新能力,平板克隆检测细胞克隆增殖能力改变。结果:无血清培养MKN28细胞,极少数细胞能形成干细胞样悬浮球,其高表达Oct4、β-catenin两个干细胞相关因子,且明显高表达PcG家族蛋白CBX7。基因转染下调CBX7表达后,胃癌细胞无血清培养成球率降低,克隆形成率下降。结论:CBX7在胃癌干细胞样细胞中高表达,其在调控胃癌干细胞自我更新和增殖能力中发挥重要作用。

关键词：色素框同源蛋白7 胃癌干细胞自我更新细胞增殖

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Multi-center clinical study of the prevalence rate of senile osteoporotic fracture in china

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Multi-center clinical study of the prevalence rate of senile...

International Conference on Osteoporosis and Bone Research 2008

作者： Zhenming Hu~1 Xianzheng Luo~2 Kerong Dai~3 Xiaolin Li~4 Tianzun Tao~5 Lihua Zhu~6 Hanchang Lao~7 Yanling zhao~8 Shou zhang~9 Fachou zhang~(10) Gang zhao~(11) Jie Hao~1 Xuesong Li~8 ~1 department of Orthopaedic Surgery The First Affiliated Hospital Chongqing Medical university Chongqing china ~2 department of Orthopaedic Surgery The Affiliated Beijing Friendship Hospital Capital Medical university Beijing china ~3 department of Orthopaedic Surgery The Affiliated shanghai 9th Hospital shanghai Jiao Tong university shanghai china ~4 department of Orthopaedic Surgery The Affiliated shanghai 6th Hospital shanghai Jiao Tong university shanghai china ~5 department of Orthopaedic Surgery The 2nd Affiliated Hospital Harbin Medical university Heilongjiang china ~6 department of Orthopaedic Surgery Nanjing Gulou Hospital Nanjing Medical university Jiangsu china ~7 department of Orthopaedic Surgery The 2nd Affiliated Hospital Kunming Medical College Yunnan china ~8 Chinese Osteoporosis Foundation of CHPF Beijing china ~9 department of Orthopaedic Surgery Haikou Peoples Hospital Hainan china ~10 department of Orthopaedic Surgery Beijing Hospital Beijing china ~11 department of Orthopaedic Surgery The 3rd Affiliated Hospital Kunming Medical College Yunnan china

＜正＞Objective:To study the prevalence of senile osteoporotic fracture in ten clinical center patients in china,in order to afford many epidemiology evidences for prevention of the different kinds of osteoporotic fra...

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A CpG ODN adjuvant enhances the protective immune response induced by a pcD/Gpd-IL-2 DNA vaccine

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A CpG ODN adjuvant enhances the protective immune response i...

2012全国临床微生物与感染免疫学术研讨会

作者： zhao Feijun~1,Liu Shuangquan~(2,3),zhang Xiaohong~4,Zeng Tiebing~1,Gu Weiming~5,Yu Jian~1,Cao Xunyu~6, Chen Xi~1,Wu Yimou~1 1.Pathogenic Biology Institute,university of South china,Hengyang City,Hunan Province 421001,china 2.department of Anatomy & Neurobiology,Xiangya School of Medicine,Central South university,Changsha, 410013,china 3.First Affiliated Hospital,university of South china,Hengyang City,Hunan Province 411000,china 4.department of Histology and Embryology,School of Medicine,university of South china,Hengyang City,Hunan Province 421001,china 5.Skin Diseases and STD Hospital,shanghai 200050,china 6.Centers for Disease Control of Hunan Province,Changsha City,Hunan province 410005,china

Objectives The protective response against Treponema pallidum(Tp) infection of a DNA vaccine enhanced by an adjuvant CpG ODN was investigated. Methods New Zealand rabbits were immunized with the eukaryotic expression recombinant pcD/Gpd-IL-2 using intramuscular multi-injection or together with mucosal enhancement via nasal *** effect of the mucosal adjuvant CpG ODN was examined. Results The mucosal adjuvant CpG ODN enhanced the production of higher levels of anti-TpGpd antibodies induced by pcD/Gpd-IL-2 in *** also resulted in higher levels of secretion of IL-2 and IFN-γ,and facilitated T cell proliferation and differentiation(P2 vaccine intramuscular injection alone(P>0.05).Furthermore,CpG ODN stimulated the production of mucosa-specific anti-sIgA antibodies and resulted in the lowest Tp-positive rate(6.7%) for Tp-infection of skin lesions and the lowest rates(8.3%) of ulceration lesions,thus achieving better protective effects. Conclusions The CpG ODN adjuvant significantly enhances the humoral and cellular immune effects of the immunization by pcD/Gpd-IL-2 with mucosal enhancement via nasal *** also stimulates strong mucosal immune effects,thus initiating more efficient immune-protective effects.

关键词： Treponema pallidum Membrane protein Tp Gpd CpG ODN DNA vaccine Mucosal immune

The MAPK pathway involves in the bone cancer pain

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The MAPK pathway involves in the bone cancer pain

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ting-Ting LI~1,Chao BIAN~1,Zhi-Qi zhao~1,Ru-Rong JI~2,Yu-Qiu zhang~(1,*) 1 Institute of neurobiology,institutes of brain science and state key laboratory of medical neurobiology,Fudan university, shanghai 200032,china 2 department of anesthesiology,Brigham and women''s hospital,Harvard medical school,Boston,Massachusetts 02115, USA

＜正＞Objective MAPKs are the evolutionally conserved molecules that play a critival role in intracellular signal transduction and consisit of extracellular signal-regulated protein kinase（ERK）,p38MAPK,c-Jun N-terminal kinase/ stress-activated protein kinase（JNK/SAPK）.It has been demonstrated that phosphorylated p38（p-p38） and phosphorylated extracellular signal-regulated kinasel/2（pERK1/2） are involved in inflammatory pain and neuropathic *** this study we futher investigate the role of two members of MAPKs in the cancer *** Walker256 mammary gland carcinoma cells were injected into the tibia medullary cavity to build a rat bone cancer pain *** method, behavioral test and histochemistry methods were used to test the development of bone cancer pain *** expression of p-p38 and pERK in the spinal dorsal horn of cancer pain model is tested by using immunohistochemistry and western blot ***（1） The significant bone destruction was observed on day 14 after the inoculation and the mechanical allodynia occurred in both hindpaws on day 8 and maintained to day 21 after inoculation.（2） p38 was activated mainly in neurons on the superficial of the spinal dorsal horn at 3 and 7 *** day 14,p-p38 was found in both neurons and microglia,but by day 21 predominantly in microglia.（3） ERK activation was found in neurons and microglia on the superficial of the spinal dorsal horn on day 3 after the ***,by 14 and 21 days after inoculation,all the neurons,astrocytes and microglia expressed pERK.（4） On day 14 after inoculation,the mechanical allodynia could be reduced by the administration of the inhibitors of p-p38 and pERK.（5） Microglia and astrocytes in the spinal dorsal horn were also activated on day 7 and lasted by day *** p-p38 and pERK as well as spinal glia cell activation are involved in bone cancer pain,and p-p38 and pERK may also play a role in spinal glia activation induced by cancer pain.

关键词： MAPK pathway spinal cord glia cells bone cancer pain

左甲状腺素钠2种配方片剂人体生物利用度比较

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中国新药与临床杂志 2005年第1期24卷 7-10页

作者：荣征星赵咏桔陈广洁王昊席晔斌张颖德陆洁莉刘玥姜昌斌陈红专上海第二医科大学药理学教研室上海200025 瑞金医院国家药品临床研究基地上海200025 上海市免疫研究所上海200025

目的:评价进口新配方与旧配方左甲状腺素钠片剂的人体相对生物利用度及生物等效性。方法:24名健康男性受试者,随机分为2组,分别于早晨空腹单剂量口服新配方或旧配方左甲状腺素钠片600μg。35d清洗期后再交叉给药。分别在服药前30,15,4mi... 详细信息

目的:评价进口新配方与旧配方左甲状腺素钠片剂的人体相对生物利用度及生物等效性。方法:24名健康男性受试者,随机分为2组,分别于早晨空腹单剂量口服新配方或旧配方左甲状腺素钠片600μg。35d清洗期后再交叉给药。分别在服药前30,15,4min内和服药后0.5,1.0,1.5,2.0,2.53.0,3.5,4.0,6.0,8.0,10.0,12.0,18.0,24.048.0h取静脉血,用放射免疫分析法测定血清样品T4和T3的浓度。结果:受试者口服新配方和旧配方左甲状腺素钠片剂后血清中T4呈明显时量变化主要药动学参数如下:tmax为(3.4±s2.3)和(3.5±2.1)h,cmax为(168±39)和(160±34)μg·L-1AUC0～48为(5640±1060)和(5620±1010)μg··L-1。而血清中T3无明显时量变化。结论:新配方和旧配方左甲状腺素钠片剂相对生物利用度为(102±18)%,2种配方的制剂具有生物等效性。

关键词：甲状腺素片剂生物利用度药物稳定性放射免疫测定左甲状腺素钠处方

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