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Anas platyrhynchos BLVRA Gene:Molecular Cloning and Correlation between Green Shell Character and mRNA Expression in the Shell Gland in the Duck

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畜牧兽医学报 2011年第B12期42卷 13-19页

作者： zhang bao-le LI Guo-qin zhang He SHEN Jun-da LI Jin-jun TAO Zheng-rong CHEN Yi-chu WANG De-qian CHEN Wen-biao SHI Fang-xiong LU Li-zhi Institute of Animal Husbandry and Veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China Jinyun Husbandry and Veterinary Bureau Jinyun 321403 China Zhejiang Wulian Agriculture And Animal Co. Ltd. Jinyun 321403 China

This study has examined the structure and function of the biliverdin reductase A (BLVRA) gene and its correlation with the green eggshell character in Jinyun ducks. The previously unknown sequences of 3' and 5' ends of duck BLVRA mRNA were cloned, using RT-PCR and 5' RACE methods with four pairs of primers designed from the known coding sequence (CDS) of the duck BLVRA gene. Gene expression levels of BLVRA in the shell gland were quantified by real-time PCR, and eggshell colors were determined objectively by the reflection coefficient. The cDNA sequence of BLVRA was comprised of 1071 nucleotides, encoding a protein of 303 amino acids with an isoelectric point of 7.15 and molecular weight of 34.3 kDa. The 5' and 3' ends of the duck BLVRA gene sequence published in the GenBank were extended by 238 and 199 bp, respectively. The deduced amino acid sequence of duck BLVRA had 95.3% identity with Gallus gallus, 95.0% with Taeniopygia guttata, 70.0% with Xenopus tropicalis,61.1% with Bos taurus, 60.5% with Homo sapiens and 59.5% with Mus musculus, which indicated that the BLVRA gene was evolutionarily conserved. The relative gene expression level of BLVRA in shell glands of Jinyun ducks with white eggshells was 3 times higher than that in ducks with dark green eggshells (Plection coefficient of eggshells and the relative expression of the BLVRA gene (r=0.719, Plecule breeding of ducks with green eggshells.

关键词：基因设计绿头鸭 mRNA表达克隆 VRA 基因表达水平 GenBank 氨基酸序列

G蛋白偶联受体3:调控神经系统和卵泡发育的关键因子

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遗传 2013年第5期35卷 578-586页

作者：张宝乐高殿帅徐银学徐州医学院神经生物学教研室徐州221002 南京农业大学动物科学技术学院南京210095

G蛋白偶联受体(G protein-coupled receptors,GPCRs)是细胞表面最大的受体超家族,参与调节多种生理和病理过程。G蛋白偶联受体3(G protein-coupled receptor 3,Gpr3)是一种新发现的鞘氨醇1-磷酸受体,它直接或者间接参与调节脊椎动物神... 详细信息

G蛋白偶联受体(G protein-coupled receptors,GPCRs)是细胞表面最大的受体超家族,参与调节多种生理和病理过程。G蛋白偶联受体3(G protein-coupled receptor 3,Gpr3)是一种新发现的鞘氨醇1-磷酸受体,它直接或者间接参与调节脊椎动物神经系统及卵泡的发育过程。作为潜在的治疗多种神经疾病和卵巢早衰的药物靶标,Gpr3的生理功能及作用的分子机制等都值得进一步研究。文章主要就Gpr3及其介导的信号通路在脊椎动物神经系统发育及卵巢卵泡发育过程中的相关作用作一综述。

关键词： G蛋白偶联受体 Gpr3 神经系统卵泡发育减数分裂

过表达Gpr3诱导猪颗粒细胞G_1阻滞及凋亡

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中国生物化学与分子生物学报 2014年第10期30卷 999-1006页

作者：张宝乐李晔徐银学徐州医学院神经生物学教研室江苏徐州221002 南京农业大学动物科学技术学院南京210095 莱阳市畜牧兽医局山东莱阳265200

G蛋白偶联受体3(Gpr3)属于G蛋白偶联受体视紫质家族成员.Gpr3通过激活Gs蛋白介导的下游信号通路,维持卵泡卵母细胞减数分裂的前期阻滞,但在卵泡颗粒细胞中的作用不清.为了明确Gpr3在猪卵泡颗粒细胞中的功能,构建了Gpr3基因的真核表达载... 详细信息

G蛋白偶联受体3(Gpr3)属于G蛋白偶联受体视紫质家族成员.Gpr3通过激活Gs蛋白介导的下游信号通路,维持卵泡卵母细胞减数分裂的前期阻滞,但在卵泡颗粒细胞中的作用不清.为了明确Gpr3在猪卵泡颗粒细胞中的功能,构建了Gpr3基因的真核表达载体,利用过表达的方式激活其介导的信号通路,并利用MTT、流式细胞术和real-time PCR等方法检测了过表达Gpr3对猪卵泡颗粒细胞增殖及凋亡的影响.结果显示,过表达Gpr3后,猪颗粒细胞的增殖水平显著下调,G0/G1期细胞的百分比增加,S期细胞减少,Cyclin B1和CDK1 mRNA的表达量也显著降低;同时,显著增加了颗粒细胞的凋亡率,在抑制Bcl-2表达的同时,促进了Bax的表达.结果表明:过表达Gpr3在猪颗粒细胞中具有抑增殖促凋亡的作用,丰富了其在调节卵泡发育过程中的生物学功能.

关键词： G蛋白偶联受体3(Gpr3) 猪颗粒细胞过表达增殖凋亡

Visible-light-responsive homologous heterostructure of oxynitrides constructed to boost charge separation and water splitting

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Science China Chemistry 2025年第01期 186-191页

作者： Hai Zou Yunfeng bao Xueshang Xin Yu Qi Tengfeng Xie Fuxiang zhang State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences School of Chemistry and Chemical Engineering Liaoning Normal University College of Chemistry Jilin University

Constructing type II heterostructures represents a widely recognized strategy for enhancing charge separation and photocatalytic efficiency,but it remains challenging to fabricate homologous heterostructures based on visible-light-responsive semiconductors,especially for popularly investigated （oxy）nitrides with low thermal ***,we synthesized a homologous heterostructure （denoted as YTON＿H） based on the oxynitrides of YTaO4-xNyand YTa *** heterostructure exhibits type II band structure alignment and intimate interface achieved through a magnesium （Mg） powder-assisted nitridation *** with widely reported heterologous heterostructures,the as-obtained YTON＿H homologous heterostructure could similarly exhibit enhanced charge separation as well as photocatalytic water splitting performance with respect to its counterparts(YTaO4-xNyand YTaON2).Meanwhile,detailed structural characterizations and spectroscopy analysis have been made to confirm the formation of heterostructure and promotion of spatial charge separation.

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A surface engineering strategy for the stabilization of zinc metal anodes with montmorillonite layers toward long-life rechargeable aqueous zinc ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 94-105页

作者： Wenbo Wang Ruifeng Xu Xu zhang Peiyu Wang bao Yang Bingjun Yang Juan Yang Kailimai Su Pengjun Ma Yanan Deng Xianfeng Fan Wanjun Chen Key Laboratory for Electronic Materials College of Electrical Engineering Northwest Minzu University Resource Chemistry and Energy Materials Research Center State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Institute for Materials and Processes School of Engineering University of Edinburgh

Rechargeable aqueous zinc-ion batteries （AZIBs） exhibit appreciable potential in the domain of electrochemical energy ***,there are serious challenges for AZIBs,for instance zinc dendrite growth,hydrogen evolution reaction （HER）,and corrosion side ***,we propose a surface engineering modification strategy for coating the montmorillonite （MMT） layer onto the surface of the Zn anode to tackle these issues,thereby achieving high cycling stability for rechargeable *** results reveal that the MMT layer on the surface of the Zn anode is able to provide ordered zincophilic channels for zinc ions migration,facilitating the reaction kinetics of zinc *** functional theory（DFT） calculations and water contact angle （CA） tests prove that MMT@Zn anode exhibits superior adsorption capacity for Zn2+and better hydrophobicity than the bare Zn anode,thereby achieving excellent cycling ***,the MMT@Zn||MMT@Zn symmetric cell holds the stable cycling over 5600 h at 0.5 mA cm-2and 0.125 m A h cm-2,even exceeding 1800 h long cycling under harsh conditions of 5 m A cm-2and 1.25 m A h *** MMT@Zn||V2O5full cell reaches over 3000 cycles at 2 A g-1with excellent rate ***,this surface engineering modification strategy for enhancing the electrochemical performance of AZIBs represents a promising application.

关键词： Surface engineering strategy Montmorillonite layer Protection mechanism of Zn anode Cycling stability Aqueous zinc ion batteries

Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

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Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen Chen Xiaolin Zeng le Wang Wenwu zhang Gang Li Xing Cheng Peiqiang Su Yong Wan Xiang Li Department of Spine Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第1期 174-180页

作者： Fan-Xiao Meng Xing-Yu Wang Ka Lu Ju-Song Yang Fu-Min zhang Ai-Jun Ma bao Du Yong-Qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University School of Chemistry and Chemical Engineering Shanghai Jiao Tong University School of Biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

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鸭蛋壳颜色定量测定与统计学分析

浙江大学学报（农业与生命科学版） 2010年第5期36卷 573-577页

作者：张宝乐任晋东李国勤石放雄卢立志浙江省农业科学院畜牧兽医研究所浙江杭州310021 南京农业大学动物科技学院江苏南京210095

以缙云麻鸭所产鸭蛋为素材,采用ECR测色仪对其4种类型鸭蛋的蛋壳颜色进行定量测定,每种类型测50个蛋,每个蛋测定3个点.结果表明：白壳、浅青壳、青壳和深青壳4组蛋的反射系数均值各组间差异极显著（P〈0.01）;利用反射系数与蛋壳颜色的... 详细信息

以缙云麻鸭所产鸭蛋为素材,采用ECR测色仪对其4种类型鸭蛋的蛋壳颜色进行定量测定,每种类型测50个蛋,每个蛋测定3个点.结果表明：白壳、浅青壳、青壳和深青壳4组蛋的反射系数均值各组间差异极显著（P〈0.01）;利用反射系数与蛋壳颜色的回归分析得出划分青壳蛋颜色层次的量化区间,即白壳蛋80.64~84.42,浅青壳蛋77.74~80.64,青壳蛋74.85~77.74,深青壳蛋64.69~74.95;对各测定点的测定值与均值的相关分析和主成分分析表明,中端为蛋壳颜色的最佳测定点,其准确性可达到0.8934;蛋壳反射系数与时间的回归分析表明,不同时间的反射系数测定值间差异不显著（P〉0.05）.

关键词：鸭 ECR测色仪蛋壳颜色定量测定蛋色均匀度统计学分析

Extraction of full energy peak of ^(137)Cs from in situ NaI(Tl)gamma-ray spectrum

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Nuclear Science and Techniques 2016年第4期27卷 53-59页

作者： le-le zhang Nan-Ping Wang bao-Chuan Li School of Geophysics and Information Technology China University of Geosciences

A levenberg–Marquardt Gaussian fitting algorithm has been used for analyzing the overlap of three peaks(the 583-ke V peak of^(208)Tl, the 609-ke V peak of214 Bi, and the 662-ke V peak of^(137)Cs) using an in situ Na I(Tl) scintillation spectrometer. The algorithm, in addition,was compared with a genetic algorithm used for multiple deconvolution. The three fitted peak areas(583, 609, and662 ke V) were calculated from the measured gamma-ray spectra obtained from a simulation experiment in which a^(137) Cs source was buried at different soil depths(from 18 to38 cm). The application of the levenberg–Marquardt algorithm yielded similar results compared to the genetic algorithm. A lack-of-fit test showed that the fitting is good when the instrumental noise levels were estimated from replicated analyses. The relative fitting error of the total net area and the residual standard deviation were within 5 %and 0.04, respectively, and the goodness of the fitting was better than 0.98. While the methods used in this paper give high performance, the results may lead to incorrect estimation when the signal-to-noise ratio is smaller than-30 d B. This study is useful for the determination of radioactive specific activity of^(137) Cs by in situ spectrometry.

关键词： 137Cs γ射线谱 Marquardt算法全能峰 Gaussian 拟合算法提取相对拟合误差