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Super-resolution microscopy based on parallel detection

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Journal of Innovative Optical Health Sciences 2019年第6期12卷 210-216页

作者： zhimin zhang Shaocong Liu Liang Xu Yubing Han Cuifang Kuang Yong Liu Xiang Hao Hongqin Yang Xu Liu State Key Laboratory of Modern Optical Instrumentation College of Optical Science and Engineering Zhejiang University HangzhouZhejiang 310027P.R.China Ningbo Research Institute Zhejiang University Ningbo 315100P.R.China Collaborative Innovation Center of Extreme Optics Shanxi University TaiyuanShanxi 030006P.R.China College of Electronics and Information Engineering Shanghai University of Electric Power Shanghai 200090P.R.China Key Laboratory of Optoelectronic Science and Technology for Medicine Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology Fujian Normal University Fuzhou 350007P.R.China

Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio(SNR)greatly[***,"Super resolution in confocal imaging,"Optik 80(2)53-54(1988).***,***,"Image scanning microscopy,"***.104(19)198101(2010).***,***,R Heintzmann,"Superresolution by image scanning microscopy using pixel reassignment,"***.38(15)28892892(2013)].Here,we use a tailor-made optical fiber and 19 avalanche pho-todiodes(APDs)as parallel detectors to upgrade our existing confocal microscopy,termed as parallel-detection super resolution(PDSR)*** order to obtain the correct shift value,we use the normalized 2D cross correlation to calculate the shifting value of each *** characterized our system performance by imaging fuorescence beads and applied this system to observing the 3D structure of biological specimen.

关键词： Pixel reassignment SIM parallel-detection super-resolution(PDSR)microscopy normalized Cros correlation algorithm

Towards a low-carbon footprint:Current status and prospects for aquaculture

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Water Biology and Security

Water Biology and Security 2024年第4期3卷 1-15页

作者： zhimin zhang Haokun Liu Junyan Jin Xiaoming Zhu Dong Han Shouqi Xie Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed Wuhan 430072China The Innovative Academy of Seed Design Chinese Academy of SciencesWuhan 430072China

Aquaculture is one of the fastest-growing agricultural sectors globally and is becoming increasingly important for producing sustainable and healthy diets with relatively low climate *** comparison to livestock production,especially beef,seafood production has lower carbon ***,some species can extract carbon from aquatic environments,contributing to an extended carbon ***,the potential for negative environmental consequences in aquaculture production might increase with the growing global population and demand for aquatic ***,we review the environmental consequences of aquaculture production and the potential effects of different aquatic products on greenhouse gas(GHG)*** also summarize approaches to improving environmental footprints for mitigating global climate *** elements,including improving feed efficiency,selecting suitable farmed species,and implementing sustainable farming practices and management in aquaculture,are discussed,as well as the trade-offs between aquaculture and capture ***,performance gaps among farmed species underscore the potential for consumer-oriented advice to significantly reduce the effects of aquaculture on climate ***,uncertainties and knowledge gaps related to carbon sequestration and GHG emissions in aquaculture ponds necessitate further ***,holistic evaluation and strategic intervention regarding GHG emissions are fundamental and essential for achieving a sustainable,low-carbon future for aquaculture.

关键词： Aquatic products Low-carbon footprints GHG emissions Aquaculture Fisheries

Fast Algorithm for Leakage Power Reduction by Input Vector Control

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Fast Algorithm for Leakage Power Reduction by Input Vector C...

Preface to Focused Issue on Discontinuous Galerkin Methods

2005 6th International Conference on ASIC

作者： Xiaotao Chang Dongrui Fan Yinhe Han zhimin zhang Xiaowei Li Institute of Computing Technology Chinese Academy of Sciences Graduate School of Chinese Academy of Sciences

ISBN: (纸本)0780392108

＜正＞With leakage power being no longer negligible,it becomes a critical problem for low power *** vector control is an effective method to reduce leakage power when a circuit enters sleep mode. It seeks to find a vector that minimizes leakage power to be statically applied to the primary inputs of a *** this paper,we present a fast algorithm to search the input vector which can lead to the minimal leakage *** order to accelerate the evaluation procedure,the circuit under simulation is reduced by circuit partition based on the level number of circuits ***,a searching algorithm based on sub-circuit is used to find the target input *** results on combinational circuits of ISCAS85 benchmark show this algorithm can accelerate calculation over 5 times.

关键词： leakage power input vector control low power design gate-level simulator

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Communications on Applied Mathematics and Computation 2022年第1期4卷 1-2页

作者： Jan S.Hesthaven Jennifer Ryan Chi-Wang Shu Jaap van der Vegt Yan Xu Qiang zhang zhimin zhang Ecole Polytechnique Federale de Lausanne(EPFL) 1015 LausanneSwitzerland Department of Applied Mathematics and Statistics Colorado School of MinesGoldenCO 80401USA Division of Applied Mathematics Brown UniversityProvidenceRI 02912USA Department of Applied Mathematics University of Twente7500AE EnschedeThe Netherlands School of Mathematical Sciences University of Science and Technology of ChinaHefei 230026AnhuiChina Department of Mathematics Nanjing UniversityNanjing 210093JiangsuChina Department of Mathematics Wayne State UniversityDetroitMI 48202USA

The discontinuous Galerkin(DG)method is a class of finite element methods using com-pletely discontinuous piecewise smooth functions(typically polynomials)as basis and test *** its inception in 1973[10],it has seen a sustained development,both in the computational mathematics community and in many scientific and engineering application *** DG methods have several advantages,such as its extreme flexibility in dealing with complex geometry and adaptive computation(both h-and p-adaptivities are easy to implement),extremely high parallel efficiency,good stability properties(energy and entropy stability has been established for DG methods in many situations),nice con-vergence and superconvergence properties,and capability to solve hyperbolic and convec-tion-dominated problems effectively.

关键词： discontinuous stability piecewise

SR9009联合吲哚丙酸通过核因子κB信号通路减轻C2C12成肌细胞的炎症反应

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中国组织工程研究 2025年第6期29卷 1220-1229页

作者：姬慧慧蒋旭张志敏邢运虹王亮亮李娜宋雨庭罗旭光崔慧林曹锡梅山西医科大学组织学与胚胎学教研室山西省晋中市030604 山西医科大学法医学院山西省晋中市030604 山西医科大学微生物免疫学教研室山西省晋中市030604 山西医科大学山西省临床级细胞治疗转化中试基地山西省晋中市030604

背景:钟基因Rev-erbα参与调节炎症,但激活Rev-erbα会增加心脑血管疾病风险。为降低相关风险,探索Rev-erbα激动剂SR9009联合其他药物来减轻骨骼肌成肌细胞炎症,奠定治疗炎症相关性骨骼肌萎缩的理论基础。目的:探讨脂多糖刺激C2C12成... 详细信息

背景:钟基因Rev-erbα参与调节炎症,但激活Rev-erbα会增加心脑血管疾病风险。为降低相关风险,探索Rev-erbα激动剂SR9009联合其他药物来减轻骨骼肌成肌细胞炎症,奠定治疗炎症相关性骨骼肌萎缩的理论基础。目的:探讨脂多糖刺激C2C12成肌细胞时吲哚丙酸、SR9009与核因子κB信号通路的关系。方法:①1μg/mL脂多糖刺激C2C12成肌细胞,RNA转录组测序结合KEGG通路富集分析信号通路。②CCK-8法检测C2C12成肌细胞活性,筛选吲哚丙酸的最佳给药浓度;然后将细胞分为空白对照组、脂多糖(1μg/mL)组、SR9009(10μmol/L)+脂多糖组、吲哚丙酸(80μmol/L)+脂多糖组、吲哚丙酸+SR9009+脂多糖组,ELISA检测细胞上清液中白细胞介素6水平,RT-qPCR检测白细胞介素6、肿瘤坏死因子α、Toll样受体4、CD14 mRNA表达,Western blot检测NF-κB p65、p-NF-κB p65蛋白表达。③siRNA敲减Rev-erbα,RT-qPCR评估敲减效率,检测白细胞介素6、肿瘤坏死因子αmRNA表达。结果与结论:①与空白对照组比较,脂多糖时间依赖性抑制成肌细胞融合形成肌管,白细胞介素6、肿瘤坏死因子αmRNA表达水平升高,细胞上清液中白细胞介素6水平显著升高;KEGG通路分析支持脂多糖刺激激活核因子κB信号通路。②吲哚丙酸浓度>80μmol/L时抑制C2C12成肌细胞活性;吲哚丙酸和SR9009通过抑制核因子κB信号通路发挥抗炎作用,降低白细胞介素6、肿瘤坏死因子α、Toll样受体4、CD14 mRNA表达水平,p-NF-κB p65/NF-κB p65蛋白表达比值低于脂多糖组。SR9009联合吲哚丙酸显著降低脂多糖诱导的炎症,Toll样受体4、CD14、白细胞介素6和肿瘤坏死因子αmRNA表达水平进一步下调,p-NF-κB p65/NF-κB p65蛋白表达比值显著低于吲哚丙酸+脂多糖组和SR9009+脂多糖组。③Rev-erbα随脂多糖刺激时间依赖性升高;siRNA敲减Rev-erbα效率达58%以上,成功敲减Rev-erbα后添加脂多糖,白细胞介素6和肿瘤坏死因子αmRNA表达较脂多糖组显著上调。④结果说明,Rev-erbα可以作为调节炎症反应的靶点,SR9009靶向激活Rev-erbα联合吲哚丙酸能抑制核因子κB信号通路显著减轻C2C12成肌细胞的炎症反应,联合抗炎效果优于单独干预。

关键词： Rev-erbα SR9009 吲哚丙酸脂多糖核因子κB信号通路 C2C12成肌细胞

Research and Application of Meteorological Response Measures for Civil Engineering and Data Acquisition System

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Meteorological and Environmental Research 2023年第3期14卷 54-59页

作者： Dawei XU zhimin zhang Feng PAN Shanghai Construction Wujian Group Co. Ltd.Shanghai 200063China

Meteorological conditions have a very important impact on the development of construction *** order to assist project site managers to quickly and accurately deal with the engineering risks brought by meteorological conditions,meteorological risk assessment indicators for civil construction are established based on the actual impact of different meteorological conditions on each sub-project of civil engineering.A meteorological intelligent management system is created,and it will select reasonable countermeasures from the integrated existing database and push them to on-site construction managers based on meteorological risk assessment *** the pre-set construction log module,the managers can give feedback on the application effect of the pushed measures,and achieve the effect of construction data collection,correction and expansion of the existing database.

关键词： Meteorology Construction measures Database Information collection Management system

High-Order Fully Discrete Energy Diminishing Evolving Surface Finite Element Methods for a Class of Geometric Curvature Flows

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Annals of Applied Mathematics 2021年第4期37卷 405-436页

作者： Beiping Duan Buyang Li zhimin zhang Beijing Computational Science Research Center Beijing 100193China Shenzhen JL Computational Science and Applied Research Institute ShenzhenGuangdong 518129China Department of Applied Mathematics The Hong Kong Polytechnic UniversityHung HornHong KongChina Department of Mathematics Wayne State UniversityDetroitMI 48202USA

This article concerns the construction of high-order energy-decaying numerical methods for gradient flows of evolving surfaces with curvature-dependent energy *** semidiscrete evolving surface finite element method is derived based on the calculus of variation of the semidiscrete surface energy *** makes the semidiscrete problem naturally inherit the energy decay *** this property,the semidiscrete problem is furthermore formulated as a gradient flow system of *** averaged vector-field collocation method is used for time discretization of the ODEs to preserve energy decay at the fully discrete level while achieving high-order accuracy in *** numerical examples are provided to illustrate the accuracy and energy diminishing property of the proposed method,as well as the effectiveness of the method in capturing singularities in the evolution of closed surfaces.

关键词： Gradient flow evolving surface curvature energy decay evolving surface finite element method averaged vector-field collocation

Effects of fish meal replacement with Chlorella meal on growth performance,pigmentation,and liver health of largemouth bass(Micropterus salmoides)

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Animal Nutrition 2022年第3期10卷 26-40页

作者： Longwei Xi Qisheng Lu Yulong Liu Jingzhi Su Wen Chen Yulong Gong Dong Han Yunxia Yang zhimin zhang Junyan Jin Haokun Liu Xiaoming Zhu Shouqi Xie State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed Wuhan 430072China The Innovative Academy of Seed Design Chinese Academy of SciencesWuhan 430072China

Chlorella meal is a potential protein source for ***,the physiological response of carnivorous fish fed Chlorella meal remains *** study evaluated the effects of replacing dietary fish meal with Chlorella meal on growth performance,pigmentation,and liver health in largemouth *** diets were formulated to replace dietary fish meal of 0%(C0,control),25%(C25),50%(C50),75%(C75),and 100%(C100)with Chlorella meal,*** 300 fish(17.6±0.03 g)were randomly assigned to 15 tanks(3 tanks/group).Fish were fed the experimental diet twice daily for 8 *** increased dietary Chlorella meal quadratically influenced the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and feed intake(FI),which were significantly lower in the C100 group than in the other groups(P<0.05).The feed conversion ratio(FCR)increased linearly or quadratically with dietary Chlorella *** Chlorella meal linearly or quadratically increased the lutein content of plasma,liver,and dorsal muscle of largemouth bass(P<0.05).Compared to the C0 group,all supplemented Chlorella meal groups significantly improved the yellowness(b*)of the dorsal body(1.5 to 2.0 fold),abdominal body(1.5 to 1.8 fold),and dorsal muscle(3.8 to 5.4 fold)of largemouth bass(P<0.05).In addition,compared to the C0 group,the liver vacuolation area of fish was significantly increased in the C75 and C100 groups(P<0.05).Transcriptional levels of apoptosis-related genes of b-cell lymphoma-2(bcl2),caspase-9-like(casp9),and caspase-3a(casp3)were markedly upregulated(0.9 to 1.6 fold)in the C100 group compared to the C0 group(P<0.05).Based on the quadratic regression analysis between FBW,WGR,or SGR and dietary Chlorella meal level,largemouth bass had the best growth when replacing 31.7%to 32.6%of fish meal with 15.03%to 15.43%dietary Chlorella *** present results indicated that dietary supplementation with Chlorella meal(11.85%to 47.45%)significantly enhanced the pigmentation;how

关键词： Chlorella meal Growth Liver health Micropterus salmoides Pigmentation

冷变形对NiTiCr形状记忆合金超弹性的影响

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稀有金属材料与工程 2008年第1期37卷 185-188页

作者：司乃潮张志敏江苏大学江苏镇江212013

通过测量NiTiCr形状记忆合金丝的恢复率和做拉伸实验来观察冷变形对其超弹性的影响。结果表明,不同的冷变形量对NiTiCr的超弹性有着显著的影响。当冷变形量超过27%时,NiTiCr丝的弹性随变形量的增加而显著增强,在变形量达到33%之后,弹性... 详细信息

通过测量NiTiCr形状记忆合金丝的恢复率和做拉伸实验来观察冷变形对其超弹性的影响。结果表明,不同的冷变形量对NiTiCr的超弹性有着显著的影响。当冷变形量超过27%时,NiTiCr丝的弹性随变形量的增加而显著增强,在变形量达到33%之后,弹性随变形量的变化不大。单纯的冷变形不能使NiTiCr丝呈现完全的非线性超弹性。同时讨论了实验弯曲次数与弹性稳定性之间的关系。

关键词：冷变形超弹性 NiTiCr