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An Evaluation of the Infection Status and Source of Subgroup J Avian Leukosis Virus in Cloned Free-Range Layers

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Journal of Integrative Agriculture 2013年第4期12卷 687-693页

作者： zhang Pei-pei LIU Shao-qiong WANG Jian WANG Bo ZHAO Cheng-di zhang yong-guang SUN Shu-hong College of Veterinary Medicine Shandong Agricultural University Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

In recent years, subgroup J avian leukosis virus （ALV-J） has been found to frequently infect layers in China. This virus is responsible for economic losses due to both mortality and decreased performance in chickens. In this study, 45-d-old cloned flee-range layers were suspected to be infected with ALV and other immunosuppressive diseases because their feathers were unkempt and their growth rate was impaired. To estimate the infection status and determine the source of ALV-J in the flock, 30 cloacal swabs were randomly collected to measure the p27 antigen level by enzyme-linked immunosorbent assay （ELISA）. Among the birds that were tested, 87% （26/30） were positive. In addition, 6 anticoagulant blood samples were aseptically collected at random from the flock when the layers were 60 d old. These samples were centrifuged to obtain the leukocytes, which were then used to inoculate chicken embryo fibroblast （CEF） cells for the identification of ALV-J by indirect immunofluorescence （IFA）. Of the samples tested, 100% （6/6） were positive. The flock＇s production performance was also investigated, and 10 layers were necropsied to evaluate pathological changes at 115 d of age. The flock never laid eggs even though they reached the age of the first laying （110 d）. Furthermore, there were pathological changes present, including atrophy of the thymus and bursa of Fabricius, undeveloped ovaries, glandular stomach haemorrhage, and hepatosplenomegaly. Paraffin-embedded sections of intumescent liver and spleen were prepared for antigen localisation using IFA. Positive signals were prevalent in paraffin-embedded sections of the intumescent liver and spleen. Furthermore, provirus DNA was extracted from 4 cloned flee-range layers, and 2 patemal parents （HR native cocks）, and the gp85 gene of ALV-J was amplified by PCR to analyse the genetic variation. The results of the autogenous variation analysis showed that the 6 strains were 98.5-99.7% homologous. This study indicated that there

关键词： cloned free-range layers ALV-J infection status hereditary variation source exploration

Hand-assisted laparoscopic splenectomy for splenomegaly:a comparative study with conventional laparoscopic splenectomy

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Chinese Medical Journal 2007年第1期120卷 41-45页

作者： WANG Ke-xin HU San-yuan zhang guang-yong CHEN Bo zhang Hai-feng Department of General Surgery Qilu Hospital of ShandongUniversity Jinan 250012 China

Background Laparoscopic splenectomy （LS） has been considered as the standard approach to remove a normal-sized spleen, but it is facing technical challenges when applied to splenomegaly. Hand-assisted laparoscopic technique was designed to facilitate the performance of difficult laparoscopic procedure. This study was aimed to evaluate the efficacy and superiority of hand-assisted laparoscopic splenectomy （HALS） for splenomegaly. Methods From November 1994 to January 2006, 36 patients with splenomegaly （final spleen weight 〉700 g） were treated with laparoscopic operations for splenectomy in our hospital. Conventional LS was performed in 16 patients （7 men and 9 women, group 1） and HALS in the other 20 patients （12 men and 8 women, group 2）. The patients＇ features, intraoperative details and the postoperative outcomes in the both groups were compared. Results The both groups were comparable in the terms of patient＇s age （（38±12） years vs （43±14）years, P〉0.05）, the greatest splenic diameter （（24±5）cm vs （27±7）cm, P〉0.05）, preoperative platelet count （（118±94）×10^9/L vs （97±81）×10^9/L P〉0.05） and diagnosis. Compared with LS group, operation time （（195±71） minutes vs （141±64） minutes, P〈0.05） was shorter, intraoperative blood loss （（138±80）ml VS （86±45）ml, P〈0.05） and conversion rate （4/16 vs 0/20, P〈0.05） were lower, but hospital stay （（5.3±3.8） days vs （7.4±1.6） days, P〈0.05） was longer in HALS group. There was no significant difference in the aspects of intraoperative and postoperative complication rate （2/16 vs 0/20, P〉0.05） or recovery time of gastrointestinal function （（16.3±11.6） hours vs （18.7±8.1）hours, p〉0.05） between the two groups. Conclusions In the cases of splenomegaly, HALS significantly facilitates the surgical procedure and reduces the operational risk, while maintaining the advantages of conventional LS. HALS is more feasible and more effective than conventional LS for the removal of splenomegaly of splenomegaly.

关键词： splenomegaly laparoscopy splenectomy surgical procedures, minimally invasive

Expression of farnesyltransferase in primary liver cancer

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Chinese Medical Journal 2012年第14期125卷 2427-2431页

作者： SUI Guo-de zhang guang-yong NIU Zhao-jian HU San-yuan Department of General Surgery Qilu Hospital of ShandongUniversity Jinan Shandong 250012 China

Background Primary liver cancer （PLC） is a common malignant tumor. Over the past decade, although farnesyltransferase （FTase） has emerged as a significant target for anticancer therapies and has become a hotspot of cancer research, its exact mechanism of action remains unknown. The aim of this study was to investigate the expression of FTase in PLC and its role in the development of PLC. Methods Expression of FTase was detected by real-time fluorescent quantitative-polymerase chain reaction （FQ-PCR） in cancer and surrounding normal tissues from 32 patients with PLC. Results Expression of FTase mRNA in PLC was significantly higher than that in normal hepatic tissues （P 〈0.001）. Overexpression of FTase was as high as 87.5%. The positive rate for FTase mRNA in the high tendency to metastatic recurrence group was obviously higher than that in the low tendency to metastatic recurrence group （P=0.02）. The positive rate for FTase mRNA in patients with metastatic recurrence during postoperative follow-up was also significantly higher than that in those without metastatic recurrence （P=-0.01）. Conclusions The level of FTase mRNA expression in cancer tissues is much higher than in normal tissues. FTase may play an important role in the genesis and development of PLC and may be one of the reliable markers for the metastatic activity gained by liver tumor cells. FTase could be used clinically in predicting metastatic recurrence of PLC

关键词： primary liver cancer, farnesyltransferase fluorescent quantitative-polymerase chain reaction

Quantum Computation Using Controllable Exchange Interactions and a Global Magnetic Field

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量子光学学报 2006年第B8期12卷 15-15页

作者： YE Ming-yong zhang yong-sheng GUO guang-can Key Laboratory of Quantum Information University of Science and Technology of China Hefei 230026 China

Protective effects of ischaemic postconditioning on warm/cold ischaemic reperfusion injury in rat liver： a comparative study with ischaemic preconditioning

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Chinese Medical Journal 2008年第20期121卷 2004-2009页

作者： WANG Ke-xin HU San-yuan JIANG Xu-sheng ZHU Min JIN Bin zhang guang-yong CHEN Bo Department of General Surgery Qilu Hospital Shandong University Jinan Shandong 250012 China

Background Ischaemic reperfusion injury （IRI） is inevitable during major liver surgery. Ischaemic preconditioning （IPC） has been proven an effective intervention against hepatic IRI. Recently, it was demonstrated that ischaemic postconditioning （IPO） provided effective cardioprotection on IRI. We evaluated the protective effects of IPO on warm/cold IRI in rat liver by a comparison with IPC and assessed the role of apoptosis in the process.Methods Warm IRI model （clamping hepatic pedicle for 30 minutes） and cold IRI model （orthotopic liver transplantation with 2 hours cold storage） were established. Each model consisted of 3 groups：（1） control group, normal warm/cold IRI; （2） IPC group, 5 minutes of ischaemia followed by 5 minutes of reperfusion twice prior to warm/cold IRI; （3） IPO group, 30 seconds of reperfusion followed by 30 seconds of reocclusion for three times after warm/cold ischaemia. The levels of serum transaminase, glucose, and γ glutamyltransferase （GGT） in bile, histopathological examination, apoptotic activity of hepatocyte, and apoptosis related protein Fas, at 3 hours after operation were compared. Survival rates one week after intervention were also compared.Results IPO and IPC protected the functions of hepatocytes and biliary epithelial cells, inhibited the hepatocellular apoptosis by preventing expression of Fas gene, and elevated the one week survival rate compared with control group in both models （P 〈0.05）. IPO and IPC groups were comparable in levels of serum transaminase levels, glucose, and GGT in bile, Fas positive expression index, and one week survival. In cold ischaemic models, IPO had lower apoptotic index than IPC （P 〈0.05）.Conclusion Compared with ischaemic preconditioning, ischaemic postconditioning is associated with comparable protections of rat liver from warm or cold ischaemic reperfusion injury.

关键词： liver ischaemic preconditioning ischaemic postconditioning reperfusion injury apoptosis

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基督教学校与近代中国体育教育

武汉体育学院学报 2006年第11期40卷 88-91页

作者：张永广华中师范大学中国近代史研究所湖北武汉430079

西方近代的体育教育是经由基督教学校传入中国的。从介绍体育教育在基督教学校的产生、发展入手,分析其开展体育教育的基本形式和内容,在此基础上探讨其教育理念和对近代中国的影响。

关键词：基督教学校体育教育近代体育中国教会学校教学管理

Improvement of Feeding Value of Rapeseed Meal by Mixed Solid State Fermentation

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Animal Husbandry and Feed Science 2010年第3期2卷 12-14,17页

作者： GU Xu-hui zhang yong-guang ZHAO Guo-ming zhang Ru-bing zhang Fan ZHU Jian-hang Key Laboratory of Biofuels Chinese Academy of Sciencesand Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China

[Objective] To optimize solid state fermentation conditions of rapeseed meal and thus to reduce glucosinolates and neutral detergent fibers by mixed cultures of Aspergillus oryzae and Tnchoderrna viride. [ Method ] The optimal fermentation conditions were determined by single factor test and orthogonal design. [ Result J The optimum fermentation conditions are as following: inoculum weight ratio (Aspergillus oryzae vs Trichoderma vinde), 1:1 ; inoculum size, 30% ; water content, 40% ; fermentation time, 96 h; and fermentation temperature, 30℃. Under these conditions, glucosinolates were reduced by 90.71% and neutral detergent fibers were degraded by 20.65%. [ Condusion] In laboratory, solid state fermentation with Aspergillus oryzae and Trichoderrna viride can be used to produce high-quality rapeseed meal.

关键词： Rapeseed meal Aspergillus oryzae Trichoderma viride Glucosinolates Neutral detergent fibers

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日本经验与民国时期中国基督教学校的立案

华中师范大学学报（人文社会科学版） 2007年第5期46卷 68-75页

作者：章开沅张永广华中师范大学中国近代史研究所湖北武汉430079

近代中日两国同为西方基督教会的重要差传地。日本基督教学校早先向政府立案的实例,构成了中国基督教教育界考虑同类问题的思想资源之一,并为中国基督教学校的立案提供了可借鉴的经验。比较两国的立案背景,它们都曾经历了反基督教运动... 详细信息

近代中日两国同为西方基督教会的重要差传地。日本基督教学校早先向政府立案的实例,构成了中国基督教教育界考虑同类问题的思想资源之一,并为中国基督教学校的立案提供了可借鉴的经验。比较两国的立案背景,它们都曾经历了反基督教运动的冲击,但在具体层面和程度上又存在不同。两国基督教学校因各自发展程度和所处的国情相异,造成双方对立案的态度不尽相同,而因此所受的冲击也差异较大。

关键词：基督教学校日本经验立案比较研究

一部具有理论创新的哲学专著——读聂暾《“中”论——中规律与中规则》

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南昌大学学报（人文社会科学版） 2010年第4期41卷 F0002-F0002,F0003页

作者：郭树森太原科技大学人文社会科学系山西太原030024 中国人民大学马克思主义学院北京100872

胡锦涛不仅向全党全社会提出了加强社会建设理论研究的重大任务，而且也论述了社会建设理论研究的方法问题。胡锦涛关于社会建设理论研究的方法论包括：关于社会建设理论研究的原则、社会建设理论研究的领域、社会建设理论研究的内容及... 详细信息

胡锦涛不仅向全党全社会提出了加强社会建设理论研究的重大任务，而且也论述了社会建设理论研究的方法问题。胡锦涛关于社会建设理论研究的方法论包括：关于社会建设理论研究的原则、社会建设理论研究的领域、社会建设理论研究的内容及社会建设理论研究的宗旨。深入学习和研究胡锦涛关于社会建设理论研究的方法，对于推进社会建设理论研究和实践创新具有重要意义。

关键词：哲学体系 “中” 理论创新规律专著《中论》魏晋时期儒家理论

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