检索结果-南通市图书馆

Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway

引用

Chinese Journal of Integrative Medicine 2023年第5期29卷 434-440页

作者： zhang Zhi-ying LIU Chao WANG Peng-xiang HAN Yi-wei zhang Yi-wen HAO Mei-li SONG Zi-xu zhang xiao-ying Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region School of MedicineXizang Minzu UniversityXianyangShaanxi Province(712082)China Key Laboratory of High Altitude Environment and Gene Related to Disease of Tibet Ministry of Education School of MedicineXizang Minzu UniversityXianyangShaanxi Province(712082)China School of Finance Economics Xizang Minzu UniversityXianyangShaanxi Province(712082)China School of Information Engineering Xizang Minzu UniversityXianyangShaanxi Province(712082)China

Objective: To investigate the effect and potential mechanism of dihydromyricetin(Dmy) on H9C2 cell proliferation, apoptosis, and autophagy. Methods: H9C2 cells were randomly divided into 7 groups, namely control, model, EV(empty p CDH-CMV-MCS-EF1-Cop GFP-T2A-Puro vector), IV(circHIPK3 interference), Dmy(50 μmol/L), Dmy+IV, and Dmy+EV groups. Cell proliferation and apoptosis were detected by cell counting kit-8 assay and flow cytometry, respectivley. Western blot was used to evaluate the levels of light chain 3 Ⅱ/Ⅰ(LC3Ⅱ/Ⅰ), phospho-phosphoinositide 3-kinase(p-PI3K), protein kinase B(p-AKT), and phospho-mammalian target of rapamycin(p-mTOR). The level of circHIPK3 was determined using reverse transcriptase polymerase chain reaction. Electron microscopy was used to observe autophagosomes in H9C2 cells. Results: Compared to H9C2 cells, the expression of circHIPK in H9C2 hypoxia model cells increased significantly(P<0.05). Compared to the control group, the cell apoptosis and autophagosomes increased, cell proliferation rate decreased significantly, and the expression of LC3Ⅱ/Ⅰ significantly increased(all P<0.05). Compared to the model group, the rate of apoptosis and autophagosomes in IV, Dmy, and Dmy+IV group decreased, the cell proliferation rate increased, and the expression of LC3Ⅱ/Ⅰ decreased significantly(all P<0.05). Compared to the control group, the expressions of p-PI3K, p-AKT, and p-mTOR in the model group significantly reduced(P<0.05), whereas after treatment with Dmy and sh-circHIPK3, the above situation was reversed(P<0.05). Conclusion: Dmy plays a protective role in H9C2 cells by inhibiting circHIPK expression and cell apoptosis and autophagy, and the mechanism may be related to PI3K/AKT/mTOR pathway.

关键词： dihydromyricetin circHIPK autophagy cell apoptosis PI3K/AKT/mTOR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Quality of Life in Terms of Mental Stress and Physical Activities among Community Elder Residents in Shenzhen in the Post-COVID-19 Period

引用

Biomedical and Environmental Sciences 2022年第12期35卷 1140-1144页

作者： zhang xiao ying DENG Shu LU Sen zhang Xu Chang NIU Tian Shui LYU Zhong Wei YANG Jian She The Affiliated Shanghai Tenth People’s Hospital of Nanjing Medical University Shanghai 200072China Shanghai Tenth People’s Hospital Tongji University School of MedicineShanghai 200072China The Second People’s Hospital of Bengbu City Bengbu 233400AnhuiChina First Affiliated Hospital of Anhui University of Science and Technology Huainan 232001AnhuiChina Longgang People’s Hospital Second Affiliated Hospital of the Chinese University of Hong KongShenzhen 518172GuangdongChina Affiliated Hangzhou Chest Hospital Zhejiang University School of MedicineHangzhou 310058ZhejiangChina

Coronavirus disease 2019(COVID-19)is a deadly infectious disease that has caused a total of 318 million cases and 5.5 million deaths as of January 14,2022[1].A series of preventive measures such as border restrictions,quarantine and isolation,and social distancing were implemented by the government,which resulted in a substantial disruption of daily life,reduced physical activity(PA).

关键词： Activities COV measures

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

补骨脂中11种活性成分在正常和糖尿病大鼠体内的药代动力学研究

引用

中国中药杂志 2024年第5期49卷 1369-1377页

作者：朱娜成丽媛张孝莹张玥周昆天津中医药大学药物安全评价中心天津301617 天津中医药大学中医药研究院天津301617

采用超高效液相色谱-串联质谱(UHPLC-MS/MS)技术同时测定正常大鼠和糖尿病模型大鼠血浆中补骨脂素、异补骨脂素、补骨脂酚、补骨脂查尔酮、补骨脂二氢黄酮甲醚、补骨脂宁、补骨脂甲素、补骨脂乙素、新补骨脂异黄酮、巴库查尔酮及corylif... 详细信息

采用超高效液相色谱-串联质谱(UHPLC-MS/MS)技术同时测定正常大鼠和糖尿病模型大鼠血浆中补骨脂素、异补骨脂素、补骨脂酚、补骨脂查尔酮、补骨脂二氢黄酮甲醚、补骨脂宁、补骨脂甲素、补骨脂乙素、新补骨脂异黄酮、巴库查尔酮及corylifol A 11种有效成分,以阐明补骨脂香豆素类、黄酮类及单萜酚类在正常和糖尿病模型大鼠体内的药代动力学特征。以高糖高脂饲料结合每2 d注射一次1%链脲佐菌素诱导2型糖尿病(T2DM)大鼠模型,灌胃给予补骨脂后采集不同时间点血浆,血浆样品经乙酸乙酯沉淀蛋白,以UHPLC-MS/MS测定血浆中补骨脂11种成分的血药浓度,采用DAS 3.0软件计算药动学参数。结果显示,雌、雄性T2DM大鼠灌胃给予补骨脂,补骨脂素、异补骨脂素、补骨脂酚、补骨脂二氢黄酮甲醚等8种受试成分的药动学行为与正常大鼠存在显著性差异。其中香豆素类成分补骨脂素、异补骨脂素、补骨脂宁在雌性与雄性T2DM大鼠体内均出现入血暴露水平降低,但黄酮类成分如补骨脂二氢黄酮甲醚、corylifol A、巴库查尔酮以及单萜酚类成分补骨脂酚在雌性T2DM大鼠体内的暴露减少,而在雄性患病大鼠体内暴露增多,提示补骨脂在临床用药时针对不同性别患者应合理调整服用剂量。

关键词：补骨脂糖尿病 UHPLC-MS/MS 药代动力学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression of p53,c-erbB-2 and Ki67 in intestinal metaplasia and gastric carcinoma

引用

World Journal of Gastroenterology 2010年第3期16卷 339-344页

作者： Zheng, Yao Wang, Lin zhang, Jian-Ping Yang, Jing-Yan Zhao, Zhu-Mei zhang, xiao-ying Shandong Univ Qilu Hosp Dept Pathol Jinan 250012 Shandong Peoples R China Shandong Univ Sch Med Dept Pathol Jinan 250012 Shandong Peoples R China

AIM:To compare two types of classification of intestinal metaplasia (IM) of the stomach and to explore their relationship to gastric carcinoma.METHODS:Forty-seven cases of gastric IM were classified into type or typeaccordingto mucin histochemical staining and compared with a novel classification in which the specimens were classif ied into simple IM (SIM) or atypical IM according to polymorphism in terms of atypical changes of the metaplastic epithelium. Forty-seven IM and thirty-seven gastric carcinoma samples were stained for p53, c-erbB-2 and Ki67 proteins by Envision immunohistochemical technique.RESULTS: There were no significant differences in the expression of p53 and c-erbB-2 among type type, typeIM and gastric carcinomas. The positive expression rate of Ki67 was significantly higher in gastric carcinomas than in type IM while no signif icant Ki67 expression differences were observed among type,typeIM and gastric carcinomas. The expression ofp53, c-erbB-2 and Ki67 proteins in 20 SIM, 27 Atypical IM and 37 gastric carcinomas showed significant differences between SIM and gastric carcinomas while no significant differences were observed between Atypical IM and gastric carcinomas.CONCLUSION: Atypical IM may better reveal the pre-cancerous nature of IM and could be a helpful indicator in the clinical follow up of patients.

关键词： Simple intestinal metaplasia p53 Atypical intestinal metaplasia c-erbB-2 Ki67

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Emergent management of penetrating trauma of aortic arch in a countryside hospital

引用

Chinese Journal of Traumatology 2011年第2期14卷 114-116页

作者： DI Dong-mei zhang xiao-ying JING Huo-jun Department of Cardiovascular Surgery Third Affiliated Hospital of Suzhou University Changzhou 213003 Jingtan Chinese Medicine Hospital Jingtan 213200 Jiangsu Province China

根据文学，仅仅关于渗透胸的主动脉的损伤的出现的一个小比例能成功地被对待。此处，我们报导我们缺乏的一所农村医院里的如此的一个病人的最近的营救的经验推进了仪器为心肺绕过操作。20 岁的男性在在无名的动脉和左普通颈动脉动脉之... 详细信息

根据文学，仅仅关于渗透胸的主动脉的损伤的出现的一个小比例能成功地被对待。此处，我们报导我们缺乏的一所农村医院里的如此的一个病人的最近的营救的经验推进了仪器为心肺绕过操作。20 岁的男性在在无名的动脉和左普通颈动脉动脉之间的大动脉的拱门上和 1.5 厘米的撕裂与破坏无名的静脉和恰好普通的颈动脉动脉为渗透的损害被招收。没有实现，病人成功地被救心肺绕过。在这种情况中的表示和管理被讨论。

关键词：主动脉弓应急管理医院农村穿透体外循环静脉

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于KPCA特征量降维的风电并网系统暂态电压稳定性评估

引用

兰州理工大学学报 2024年第2期50卷 96-103页

作者：张晓英史冬雪张琎张鑫兰州理工大学电气工程与信息工程学院甘肃兰州730050 国网甘肃省电力公司陇南供电公司甘肃陇南746000 国网甘肃省电力公司超高压公司甘肃兰州730000

针对电力系统暂态电压稳定性评估中所需特征量数据庞大,影响模型训练时间,降低计算效率等问题,提出了一种基于核主成分分析方法KPCA和CPSO-BP组合的风电并网系统暂态电压稳定性评估方法.首先根据输入特征采集原始特征集,采用核主成分分... 详细信息

针对电力系统暂态电压稳定性评估中所需特征量数据庞大,影响模型训练时间,降低计算效率等问题,提出了一种基于核主成分分析方法KPCA和CPSO-BP组合的风电并网系统暂态电压稳定性评估方法.首先根据输入特征采集原始特征集,采用核主成分分析算法对特征量进行非线性数据处理,提取出最优的特征集.然后将降维后的特征集作为CPSO-BP神经网络输入量进行监督学习,将得到的模型按照临界故障切除时间裕度值的大小进行分类,将分类后的样本进行风电并网系统的暂态电压稳定性评估和临界故障切除时间裕度值预测.仿真分析结果表明,对输入特征进行降维,保留重要输入特征量,剔除冗余特征量,不仅简化了模型,还提高了网络评估的准确性和计算效率.

关键词：风电并网核主成分分析算法降维 CPSO-BP神经网络暂态电压稳定性评估

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Theoretical Approximation of Focusing-Wave Induced Load upon A Large-Scale Vertical Cylinder

引用

China Ocean Engineering 2017年第5期31卷 598-606页

作者： XUE Hong-xiang HU Zhe TANG Wen-yong zhang xiao-ying WANG Kun-peng State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Collaborative Innovation Center for Advanced Ship and Deep-Sea Exploration Key Laboratory of Ships and Ocean Engineering of Fujian Province Jimei University School of Naval Architecture and Ocean Engineering Jiangsu University of Science and Technology

Until now, most researches into the rogue-wave-structure interaction have relied on experimental measurement and numerical simulation. Owing to the complexity of the physical mechanism of rogue waves, theoretical study on the wave-structure issue still makes little progress. In this paper, the rogue wave flow around a vertical cylinder is analytically studied within the scope of the potential theory. The rogue wave is modeled by the Gauss envelope, which is one particular case of the well-known focusing theory. The formulae of the wave-induced horizontal force and bending moment are proposed. For the convenience of engineering application, the derived formulae are simplified appropriately, and verified against numerical results. In addition, the influence of wave parameters, such as the energy focusing degree and the wave focusing position, is thoroughly investigated.

关键词： rogue wave Gauss envelope upright cylinder potential theory analytical approximation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

氧分析仪常见故障分析及应用

引用

云南冶金 2024年第2期53卷 156-160页

作者：张潇莹中铜(昆明)铜业有限公司云南昆明650502

介绍了LECO RO500氧分析仪测试氧含量的工作原理,对该仪器在检测铜杆氧含量过程中出现仪器漏气检查未通过、分析时自动终止、分析功率不足、分析曲线异常等四类常见故障的具体原因进行了分析,并采取了相应的控制措施,结果表明,制定的四... 详细信息

介绍了LECO RO500氧分析仪测试氧含量的工作原理,对该仪器在检测铜杆氧含量过程中出现仪器漏气检查未通过、分析时自动终止、分析功率不足、分析曲线异常等四类常见故障的具体原因进行了分析,并采取了相应的控制措施,结果表明,制定的四种常见故障控制措施有效,氧分析仪的平均故障处理时间从4 h降低到1 h。

关键词：氧分析仪氧含量故障平均故障处理时间

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于代谢组学的补骨脂素在2D和3D培养模型的肝毒性差异研究

引用

中国药理学通报 2024年第2期40卷 399-400页

作者：蒲位凌夏宁张孝莹张玥周昆天津中医药大学中医药研究院天津301617 天津中医药大学组分中药国家重点实验室天津301617 天津中医药大学现代中医药海河实验室天津301617

中药补骨脂具有温肾助阳,纳气等作用,主治阳痿遗精,腰膝冷痛,肾虚作喘等。补骨脂及其方剂的不当使用有造成肝损伤的风险。补骨脂素是补骨脂药材的主要活性成分之一,同时也是导致补骨脂肝毒性的主要成分之一[1]。体外毒性实验具有速度快... 详细信息

中药补骨脂具有温肾助阳,纳气等作用,主治阳痿遗精,腰膝冷痛,肾虚作喘等。补骨脂及其方剂的不当使用有造成肝损伤的风险。补骨脂素是补骨脂药材的主要活性成分之一,同时也是导致补骨脂肝毒性的主要成分之一[1]。体外毒性实验具有速度快、成本低、通量高等优势。

关键词：补骨脂素肝毒性 3D培养代谢组学药物敏感性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design, Synthesis and Antitumor Activity of a Novel Series of PAC-1 Analogues

引用

Chemical Research in Chinese Universities 2013年第5期29卷 906-910页

作者： LUO Hao-ming YANG Chun-ling zhang xiao-ying ZHAO Ming-ming JIANG Dan xiao Jun-hai YANG xiao-hong LI Song School of Pharmaceutical Sciences Jilin University Changchun 130021 P. R. China National Engineering Research Center for the Strategic Drugs Beijing Institute of Pharmacology and Toxicology Beo'ing 100850 P. R. China The First Hospital of Jilin University Changchun 130021 P. R. China School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 P. R. China

A novel series of first procaspase activating compound（PAC-1） analogues was designed, synthesized and evaluated for antitumor activity towards two cell lines[human promyelocytic leukemia cell line（HL60） and human embryonic lung fibroblast cell line（HLF）] by the MTT[3-（4,5）-dimethylthiahiazo（-z-yl）-3,5-di-phenytetrazo- liumromide] method in vitro. The structures of all the compounds were confirmed by IH NMR, MS and elemental analysis. Among the compounds synthesized^（E）-2-[（3-{[4-（tert-buty~）benzy~]（methy~）amin~}pr^py~）（methy~）amin~]- N＇-[4-（diethylamino）-2-hydroxybenzylidene]acetohydrazide（compound 6n） exhibits a good anti-proliferative activity to the majority of tumor cells tested, and selectively cleaves cancer cells. Thus, compound 6n was identified as promising lead compound for further structural modification.

关键词： PAC-1 analogue Anticancer activity MTT assay HL60

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：