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妊娠中期和后期牦牛胎盘组织中ELANE表达、定位及功能分析

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农业生物技术学报 2024年第10期32卷 2275-2284页

作者：陈文丽张博皓李建富袁宝周斌张勇赵兴绪张全伟甘肃农业大学生命科学技术学院甘肃省动物生殖生理及繁殖调控重点实验室甘肃农业大学动物医学院

哺乳动物妊娠和分娩过程与胎盘炎症发生和发展密切，炎症反应过早或免疫失衡可导致妊娠终止或流产。中性粒细胞弹性蛋白酶(elastase, neutrophil expressed, ELANE)在炎症发生和发展过程中发挥重要作用。本研究旨在探究妊娠中期和后期牦... 详细信息

哺乳动物妊娠和分娩过程与胎盘炎症发生和发展密切，炎症反应过早或免疫失衡可导致妊娠终止或流产。中性粒细胞弹性蛋白酶(elastase, neutrophil expressed, ELANE)在炎症发生和发展过程中发挥重要作用。本研究旨在探究妊娠中期和后期牦牛(Bos grunniens)胎盘组织中ELANE定位、表达规律及潜在功能。采集妊娠中期和后期牦牛胎盘组织(n=3/组)，通过苏木精-伊红(hematoxylin-eosin, HE)染色、免疫组织化学(immunohistochemistry, IHC)染色、免疫荧光(immunofluorescence, IF)染色、实时荧光定量PCR (real-time fluorescence quantitative PCR, qRT-PCR)和蛋白免疫印迹(Western blot)分析ELANE的定位及表达；基于数据非依赖型模式(data-independent acquisition, DIA)蛋白质组学数据预测牦牛胎盘中ELANE潜在生物学功能。HE显示妊娠中期牦牛胎盘组织可见大量滋养层巨细胞和单核滋养层细胞；妊娠后期滋养层细胞数目减少。IHC和IF表明，ELANE蛋白主要定位于牦牛胎盘滋养层巨细胞和单核滋养层细胞胞质。qRT-PCR和Western blot显示，与妊娠中期相比，妊娠后期牦牛胎盘组织ELANE基因及其蛋白表达显著上调(P<0.01)。生物信息学分析发现，ELANE可能通过吞噬作用、细胞外空间的组成、丝氨酸型内肽酶活性和与细胞因子结合等途径调控妊娠中期和后期牦牛胎盘的生长发育。综上，ELANE可能通过调节牦牛胎盘滋养层细胞的功能调控胎盘动态发育和妊娠维持。本研究为深入探讨牦牛胎盘组织ELANE功能提供参考。

关键词：胎盘中性粒细胞弹性蛋白酶(ELANE) 表达定位数据非依赖型模式(DIA)蛋白质组学

Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第1期12卷 55-64页

作者： wei zhang quan-wei wei Zheng-chao WANG wei DING wei WANG Fang-xiong SHI Laboratory of Animal Reproduction College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China

Objective: The present study is designed to investigate the cellular expressions and immunolocalizations of three different nitric oxide synthase (NOS) isoforms and the related nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) signaling pathway in the ovaries of neonatal and immature ***: The ovaries were obtained from ICR (Institute for Cancer Research) female Sprague-Dawley rats at postnatal days 1,5,7,10,and *** we carried out the histologic examination,immunohistochemistry,measurement of NOS activity,and modifications within the NO/cGMP ***: During postnatal days 1,5,7,10,and 19,all three isoforms of NOS were mainly localized to the oocytes and expressed as a gradual increase in granulosa cells and theca cells within the growing *** ovarian total NOS activities and NO levels were increased at postnatal days 7 and 10 compared with other ***: Our findings suggest that the locally produced NO and the NO/NOS signaling systems are involved in the follicular development to puberty.

关键词： Nitric oxide synthase Nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) pathway Ovary Neonatal rats Prepuberty

牦牛STAG3基因克隆及其在不同年龄段牦牛睾丸中的表达定位

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农业生物技术学报 2024年第2期32卷 344-354页

作者：石军陈文丽白旭李建富袁宝赵兴绪张全伟甘肃农业大学生命科学技术学院甘肃省动物生殖生理及繁殖调控重点实验室甘肃农业大学动物医学院

基质抗原3(stromal antigen 3, STAG3)是黏连蛋白复合体的重要成分之一，在动物卵泡发育、卵巢早衰和癌症发生等生物学过程中发挥重要调节作用，其在牦牛(Bos grunniens)生殖和发育过程中的作用尚不明确。本研究以不同岁龄(2, 4, 6和8岁... 详细信息

基质抗原3(stromal antigen 3, STAG3)是黏连蛋白复合体的重要成分之一，在动物卵泡发育、卵巢早衰和癌症发生等生物学过程中发挥重要调节作用，其在牦牛(Bos grunniens)生殖和发育过程中的作用尚不明确。本研究以不同岁龄(2, 4, 6和8岁龄)牦牛睾丸组织为材料，通过反转录PCR(reverse transcription-PCR, RT-PCR)克隆STAG3基因编码区序列(CDS)，利用STRING蛋白质互作数据库(STRING:functional protein association networks)和基因本体(Gene Ontology, GO)预测分析STAG3互作蛋白及生物学功能；通过苏木精-伊红(hematoxylin-eosin, HE)染色观察不同年龄牦牛睾丸组织形态结构；免疫组织化学(immunohistochemistry, IHC)染色和qRT-PCR分析STAG3分布及表达规律。结果显示，牦牛STAG3基因CDS区全长3 679 bp，开放阅读框为1 659 bp，编码552个氨基酸，蛋白无跨膜结构区，可能参与第一次减数分裂、联会复合和染色体浓缩等生物学过程；STAG3蛋白在各年龄段牦牛睾丸组织中均有表达，主要定位于初级精母细胞和次级精母细胞；2岁龄牦牛睾丸组织STAG3表达最高，且随年龄增长表达逐渐降低。结果表明，STAG3在动物进化中较为保守，各年龄段牦牛睾丸组织均有表达，其可能在牦牛生殖过程中发挥重要作用。本研究为解析牦牛生殖过程中STAG3功能及调控机制提供了参考依据。

关键词：牦牛基质抗原3(STAG3) 基因克隆生物信息学分析

Identification of novel and differentially expressed micro RNAs in ovine ovary and testis tissues using Solexa sequencing and bioinformatics

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Journal of Integrative Agriculture 2015年第8期14卷 1604-1616页

作者： CHANG wei-hua zhang Yong CHENG zhang-rui ZHAO Xing-xu WANG Juan-hong MA You-ji HU Jun-jie zhang quan-wei College of Veterinary Medicine Gansu Agricultural University College of Animal Science Tarim University Royal Veterinary College London University College of Animal Science and Technology Gansu Agricultural University

Micro RNAs（mi RNAs） are small, single stranded, non-coding RNA molecules, about 19–25 nucleotides in length, which regulate the development and functions of reproductive system of *** discover novel mi RNAs and identify the differential expression of them in ovine ovary and testis tissues, the study constructed two libraries by using next-generation sequencing technologies（Solexa high-throughput sequencing technique）.As a result, 9 321 775 and 9 511 538 clean reads were obtained from the ovary and testis separately, which included 130 562（90 genes of ovary） and 56 272（85 genes of testis） of known mi RNAs and 486 potential novel mi RNAs *** this study, a total of 65 conserved mi RNAs were significantly differentially expressed（P〈0.01） between the two *** them, 28 mi RNAs were up-regulated and 3 mi RNAs were down-regulated on ovary compared with *** addition, the known mi RNAs with the highest expression level（5 mi RNAs） and 30 novel mi RNAs with the functions related to reproduction were validated using the real-time quantitative ***, the gene ontology（GO） annotation and Kyoto encyclopedia of genes and genomes（KEGG） pathway analysis showed that differentially expressed mi RNAs were involved in ovary and testis physiology, including signal transduction, gonad development, sex differentiation, gematogenesis, fertilization and embryo *** results will be helpful to facilitate studies on the regulation of mi RNAs during ruminant reproduction.

关键词： microRNA ovary testis solexa sequencing qRT-PCR

Selective adsorption of tetracycline by β-CD-immobilized sodium alginate aerogel coupled with ultrafiltration for reclaimed water

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Chinese Journal of Chemical Engineering 2024年第4期68卷 27-34页

作者： Xi quan Jun zhang Linlin Yin wei Zuo Yu Tian State Key Laboratory of Urban Water Resource and Environment(SKLUWRE) School of EnvironmentHarbin Institute of TechnologyHarbin 150090China

In this work,a novel composite material based on β-cyclodextrin-immobilized sodium alginate aerogel(β-CD/NaAlg) was developed utilizing cross-linker of epichlorohydrin and applied as an adsorbent to remove tetracycline antibiotics from reclaimed wastewater.A series of characterizations were utilized to confirm the successful synthesis of the adsorbent and this β-CD/NaAlg presented a three-dimensional network at the nanoscale or *** optimal conditions(pH=4,t=8 h,β-CD:NaAlg=9,adsorbent dosage = 1.5 g·L-1),the maximum removal rate of β-CD/NaAlg to tetracycline was 70%.The adsorption behavior of tetracycline on β-CD/NaAlg conformed to the Freundlich isotherm model(R2=0.9977) and the pseudo-second-order kinetic model(R^(2)=0.9993).Moreover,the adsorbent still removed 55.3% of tetracycline after five ***,the adsorbent was integrated with ultrafiltration to adsorb tetracycline antibiotics from simulated reclaimed wastewater,and the removal rate of tetracycline reached 78.9% within 2 *** existence of Cr(Ⅵ) had a negligible impact on tetracycline removal,while the presence of humic acid exhibited a promoting *** possible adsorption mechanisms were also elucidated through X-ray photoelectron spectroscopy and density functional theory *** summary,β-CD/NaAlg represents an environmentally friendly,efficient,and sustainable adsorbent for removing tetracycline antibiotics from reclaimed water.

关键词： b-CD/NaAlg aerogel Covalent grafting Tetracycline antibiotics Ultrafiltration Selective adsorption Reclaimed water

Numerical simulation of transient characteristics in a bulb turbine during the load rejection process

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Journal of Hydrodynamics 2024年第1期36卷 170-183页

作者： Yue Lu Yu-quan zhang Zhong-wei He Yuan Zheng College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing210098China College of Energy and Electrical Engineering Hohai UniversityNanjing210098China Huadong Engineering Corporation Ltd. Hangzhou311122China

To evaluate the safety of the bulb tubular turbine,the dynamic hydraulic characteristics of a hydropower station system during the load rejection process are studied through numerical simulations and a prototype *** the developed model,a dynamic grid technology(DGT)controls the closure of the guide vane and the blade,whilst the moment balance equation and the user-defined function(UDF)provide the runner’s rotation *** 3-D transient simulation method can well predict the rotation speed and mass flow curves in the state of load *** simulation outcomes of the system performance are basically consistent with the measurement data of the *** observed,the runner is subjected to the reversely increased torque and axial force,the system is in a braking phase,and the maximum speed peaks at 144.6%of the rated ***,the internal flow of the runner is greatly affected by the closure of the guide vane,and the draft tube forms an eccentric spiral vortex *** breaks downstream,aggravating the instability of the draft ***,the transient characteristics span for the first five seconds,demonstrating the importance of establishing an efficient governing *** obtained results are useful for designing the turbine’s flow channel with a double regulating function and comprehending the turbine’s transient characteristics.

关键词： Bulb tubular turbine load rejection numerical simulation dynamic grid prototype test

Conductive and stable polyphenylene/CNT composite membrane for electrically enhanced membrane fouling mitigation

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Frontiers of Environmental Science & Engineering 2024年第1期18卷 35-47页

作者： Huijuan Xie Haiguang zhang Xu Wang Gaoliang wei Shuo Chen Xie quan Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education) School of Environmental Science and TechnologyDalian University of TechnologyDalian 116024China

Nanocarbon-based conductive membranes, especially carbon nanotube (CNT)-based membranes, have tremendous potential for wastewater treatment and water purification because of their excellent water permeability and selectivity, as well as their electrochemically enhanced performance (e.g., improved antifouling ability). However, it remains challenging to prepare CNT membranes with high structural stability and high electrical conductivity. In this study, a highly electroconductive and structurally stable polyphenylene/CNT (PP/CNT) composite membrane was prepared by electropolymerizing biphenyl on a CNT hollow fiber membrane. The PP/CNT membrane showed 3.4 and 5.0 times higher electrical conductivity than pure CNT and poly(vinyl alcohol)/CNT (PVA/CNT) membranes, respectively. The structural stability of the membrane was superior to that of the pure CNT membrane and comparable to that of the PVA/CNT membrane. The membrane fouling was significantly alleviated under an electrical assistance of −2.0 V, with a flux loss of only 11.7% after 5 h filtration of humic acid, which is significantly lower than those of PP/CNT membranes without electro-assistance (56.8%) and commercial polyvinylidene fluoride (PVDF) membranes (64.1%). Additionally, the rejection of negatively charged pollutants (humic acid and sodium alginate) was improved by the enhanced electrostatic repulsion. After four consecutive filtration-cleaning cycle tests, the flux recovery rate after backwashing reached 97.2%, which was much higher than those of electricity-free PP/CNT membranes (67.0%) and commercial PVDF membranes (61.1%). This study offers insights into the preparation of stable conductive membranes for membrane fouling control in potential water treatment applications.

关键词： Polyphenylene CNTs Membrane Electro-assistance Membrane fouling mitigation

Transfer matrix method for free and forced vibrations of multi-level functionally graded material stepped beams with different boundary conditions

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Applied Mathematics and Mechanics(English Edition) 2024年第6期45卷 983-1000页

作者： Xiaoyang SU Tong HU wei zhang Houjun KANG Yunyue CONG quan YUAN School of Civil Engineering and Architecture Guangxi UniversityNanning 530004China Scientific Research Center of Engineering Mechanics Guangxi UniversityNanning 530004China State Key Laboratory of Featured Metal Materials and Life-Cycle Safety for Composite Structures Guangxi UniversityNanning 530004China

Functionally graded materials(FGMs)are a novel class of composite materials that have attracted significant attention in the field of engineering due to their unique mechanical *** study aims to explore the dynamic behaviors of an FGM stepped beam with different boundary conditions based on an efficient solving *** the assumptions of the Euler-Bernoulli beam theory,the governing differential equations of an individual FGM beam are derived with Hamilton’s principle and decoupled via the separation-of-variable ***,the free and forced vibrations of the FGM stepped beam are solved with the transfer matrix method(TMM).Two models,i.e.,a three-level FGM stepped beam and a five-level FGM stepped beam,are considered,and their natural frequencies and mode shapes are *** demonstrate the validity of the method in this paper,the simulation results by ABAQUS are also *** this basis,the detailed parametric analyses on the frequencies and dynamic responses of the three-level FGM stepped beam are carried *** results show the accuracy and efficiency of the TMM.

关键词： transfer matrix method(TMM) free vibration forced vibration functionally graded material(FGM) stepped beam

Evaluation on Chinese Bread Wheat Landraces for Low pH and Aluminum Tolerance Using Hydroponic Screening

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Agricultural Sciences in China 2009年第3期8卷 285-292页

作者： DAI Shou-fen YAN Ze-hong LIU Deng-cai zhang Lian-quan wei Yu-ming ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University Dujiangyan 611830 P.R.China

Aluminum （Al） toxicity often takes place in acidic soils with a pH of 5.5 or lower. Breeding and cultivation of Al tolerance wheat can partially protect wheat escaping from Al toxicity. The scarcity of the tolerant sources impedes the wheat breeding. In order to find new Al tolerance sources, we screened 173 bread wheat landraces from Tibet of China using hydroponic screening. It was indicated that：（1） There were diversities on the root regenerate length （RRL）. The RRL of a large of landraces were longer than 7.00 cm in pH 7 （58.38%） and pH 4.5 （66.47%）, but shorter than 5.00 cm in pH 4.5 ＋50μM Al^3＋（80.93%）. The low pH showed either promotion or restraining effects depend on landraces, but Al toxicity under low pH only showed restraining effects on the root elongation. （2） There were also diversities on root tolerance index of low pH （RTI 1） or root aluminum tolerance index （RTI2） among cultivars. The RTI1 varied from a narrow range but with relatively high value （0.8722-1.2953） in comparison with that of RTI2 （0.3829-1.0058）, and the RTI1 of approximately 60% landraces was higher than 1.0000, the RTI2 of only 19.07% landraces was higher than 0.7000, suggesting that Al toxicity acted as an important factor for the reduction of the root elongation under acidic soils. （3） The RTI 1 of many wheats was higher than 1.0000, and As2256 and As2295 were the most tolerant for low pH, with RTI1 1.2953 and 1.2925, respectively. （4） Based on RTI2, seven wheats showed similar or higher tolerance to Al toxicity than Chinese Spring （CS）, a known tolerance wheat. Much better tolerance existed in landraces of As1543 and As1242, which can be used as the new parents for Al tolerant breeding.

关键词： bread wheat root regenerate length tolerance index aluminum tolerance hydroponic screening