检索结果-南通市图书馆

Effects of proteinase A on cultivation and viability characteristics of industrial Saccharomyces cerevisiae WZ65

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第10期10卷 769-776页

作者： Hong-bo zhang Hai-feng zhang qi-he CHEN hui RUAN Ming-liang FU Guo-qing HE Department of Food Science and Nutrition Zhejiang University Hangzhou 310029 China

Proteinase A （PrA）, encoded by PEP4 gene, is a key enzyme in the vacuoles of Saccharomyces cerevisiae. We characterized the effects of PrA on cell growth and glucose metabolism in the industrial S. cerevisiae WZ65. It was observed that the lag phase of cell growth of partial PEP4 gene deletion mutant （36 h） and PrA-negative mutant （48 h） was significantly extended, compared with the wild type strain （24 h）（P〈0.05）, but PrA had no effect on glucose metabolism either under shaking or steady state cultivations. The logistic model was chosen to evaluate the effect of PrA on S. cerevisiae cell growth, and PrA was found to promote cell growth against insufficient oxygen condition in steady state cultivation, but had no effect in shaking cultivation. The effects of glucose starvation on cell growth of partial PEP4 gene deletion strain and PrA-negative mutant were also evaluated. The results show that PrA partial deficiency increased the adaption ofS. cerevisiae to unfavorable nutrient environment, but had no effect on glucose metabolism under the stress of low glucose. During heat shock test, at 60 ℃ the reduced cell viability rate （RCVR） was 10% for the wild type S. cerevisiae and 90% for both mutant strains （P〈0.01）, suggesting that PrA was a negative factor for S. cerevisiae cells to survive under heat shock. As temperatures rose from 60 ℃ to 70℃, the wild type S. cerevisiae had significantly lower relative glucose consumption rate （RGCR）（61.0% and 80.0%） than the partial mutant （78.0% and 98.5%） and the complete mutant （80.0% and 98.0%）（P〈0.05）, suggesting that, in coping with heat shock, cells of the PrA mutants increased their glucose consumption to survive. The present study may provide meaningful information for brewing industry; however, the role of PrA in industrial S. cerevisiae physiology is complex and needs to be further investigated.

关键词： Proteinase A (PrA) PEP4 gene Saccharomyces cerevisiae WZ65 Cell metabolism Viability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Human responses to flower fragrance of Lilium ‘Siberia’ and Rosa ‘Escimo’

引用

Forestry Studies in China 2009年第3期11卷 185-189页

作者： JIN Zi-lin LI Xia zhang qi-xiang PAN hui-tang AN Xue National Engineering Research Center for Floriculture Beijing Forestry University Beijing 100083 R R. China

The objective of our study was to lay a foundation for the effect of flower flagrance on human emotions and to provide a theory for the choice of indoor plants and the improvement of the olfactory environment. Specifically, our purpose was to study human physiological responses to flower fragrance of Lilium ＇Siberia＇ and Rosa ＇Escimo＇. The participants were 31 college students. Blood pressure, pulse rate, finger temperature （FT） and galvanic skin response （GSR） were measured. The results show that the fragrance of Rosa ＇Escimo＇ causes the diastolic pressure and pulse rate of the participants to reduce significantly. The average decrease was 0.37 kPa and 2.23 beats per minute, which indicates that sympathetic nervous activity increases, physiological arousal decreases and emotional alleviation occurs. Furthermore, the GSR of participants significantly increased by smelling the fragrance of Lilium ＇Siberia＇, indicating that both sympathetic nervous activity and physiological arousal increased. But the data could not prove that flower fragrance stimulation has an effect on changes in systolic pressure and finger temperature. Some other factors, such as basic emotion and weather, may have an effect.

关键词： Lilium 'Siberia' Rosa 'Escimo' flower fragrance physiology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

五伦五德源流

引用

河南师范大学学报（哲学社会科学版） 2015年第3期42卷 82-87页

作者：章启辉湖南大学岳麓书院湖南长沙410082

五伦五德源远流长,经历了三代春秋源起、战国秦汉之际初形、两汉完型和立法三个历史阶段,形成了五伦五常、三纲五常两个思想体系。这一过程是五伦五德理论体系的形成过程,也是中国古代社会核心价值观的形成过程。在这个漫长的理论演变... 详细信息

五伦五德源远流长,经历了三代春秋源起、战国秦汉之际初形、两汉完型和立法三个历史阶段,形成了五伦五常、三纲五常两个思想体系。这一过程是五伦五德理论体系的形成过程,也是中国古代社会核心价值观的形成过程。在这个漫长的理论演变过程中,孔子是理论奠基的代表,孟子是理论伦常化的代表,董仲舒是理论纲常化的代表,《白虎通德论》是理论完型和立法的标识。

关键词：五伦五德五伦五常三纲五常

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design of BLDCM Driving and Control System for Motorized Treadmill

引用

Journal of Electronic Science and Technology of China 2007年第2期5卷 141-145页

作者： qi zhang hui Li Li-Bin Wang The authors are with School of Mechatronics Engineering University of Electronic Science and Technology of China （UESTC） Chengdu 610054 China

To satisfy the requirement of developing a new generation of motorized treadmill for a famous domestic manufacturer, a brushless DC motor （BLDCM） driving and control system for motorized treadmill is developed. High integration and reliability of this system are ensured under the condition that intelligent power module （IPM） is used and the protection module is included. Periodic current control method is applied to reduce the average current flowing through the armature winding of the motor when the treadmill is required to start with low speed while large load is added. Piecewise proportion-integration-differentiation （PID） control algorithm is applied to solve the problem of speed fluctuation when impulse load is added. The motorized treadmill of a new generation with the driving and control system has the advantages of high reliability, good speed stability, wide timing scope, low cost, and long life-span. And it is very promising for practical applications.

关键词： Brushless DC motor intelligent power module motorized treadmill periodic current control method.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reflector Design Method for Rotational Uniform Illuminance System with LED

引用

Semiconductor Photonics and Technology 2009年第3期15卷 163-166页

作者： zhang qi-hui WANG Hong JI Ling-ling Engineering Research Center for Semiconductor Lighting Guangdong University Guangzhou 510640CHN School of Science South China University of Technology Guangzhou 510640 CHN

Based on nonimaging design method, uniform illuminance systems with LED source were developed to create a uniform illuminated circular region with a desired size in a screen at a prescribed place. By using ray-tracing software based on Monte-Carlo method, the simulation results show that in the illuminated region the luminous uniformity is higher than 90%.

关键词： semiconductor lighting nonimaging optics uniform illuminance system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

传统儒家实学与现代国家软实力理论

引用

湖南大学学报（社会科学版） 2012年第1期26卷 119-122页

作者：章启辉冯祺湖南大学岳麓书院湖南长沙410082

传统儒家实学是中国传统实学的重要组成部分,包括先秦以降儒学的实学精神和清代儒学的实学形态。现代国家软实力理论是西方国际政治学新自由主义派的新成果。以国家软实力为视界,传统儒家实学是中国古典国家软实力理论,对于现代国家软... 详细信息

传统儒家实学是中国传统实学的重要组成部分,包括先秦以降儒学的实学精神和清代儒学的实学形态。现代国家软实力理论是西方国际政治学新自由主义派的新成果。以国家软实力为视界,传统儒家实学是中国古典国家软实力理论,对于现代国家软实力理论具有历史借鉴意义。文亦提出:传统儒家实学以明体达用、崇实黜虚为特征,相对之"虚学"以体用分离、空谈形上为特征;儒道佛三学理一分殊,殊途同归,不为对立,而为互补;中国传统实学包括儒道佛,不能独判儒学为实而以道学和佛学为虚。

关键词：儒家实学国家软实力

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

论罗近溪“不学不虑”之学

引用

湖南大学学报（社会科学版） 2012年第4期26卷 39-43页

作者：阮春晖章启辉湖南大学岳麓书院湖南长沙410082

近溪"不学不虑"之学可从三个方面展开:"赤子之心"是立其体,"孝弟慈"是贯其实,"当下理趣"是受其用,三者循环互解又自成一体。在识取道德源头圆满自足的前提下,"不学不虑"呈显为当下具体事为,当下情境凝一成"不学不虑"之理境。

关键词：罗近溪赤子之心孝弟慈当下理趣不学不虑

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Field mold stress induced catabolism of storage reserves in soybean seed and the resulting deterioration of seed quality in the field

引用

Journal of Integrative Agriculture 2022年第2期21卷 336-350页

作者： DENG Jun-cai LI Xiao-man XIAO Xin-li WU Hai-jun YANG Cai-qiong LONG Xi-yang zhang qi-hui Nasir IQBAL WANG Xiao-chun YONG Tai-wen DU Jun-bo YANG Feng LIU Wei-guo zhang Jing WU Xiao-ling WU Yu-shan YANG Wen-yu LIU Jiang Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Ecophysiology and Farming System in Southwest Ministry of Agriculture and Rural AffairsChengdu 611130P.R.China College of Agriculture Sichuan Agricultural UniversityChengdu 611130P.R.China Institute of Ecological Agriculture Sichuan Agricultural UniversityChengdu 611130P.R.China College of Horticulture Sichuan Agricultural UniversityChengdu 611130P.R.China School of Agriculture Food and WineThe University of AdelaideUrrbraeSA 5064Australia

Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold(FM) stress. If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality. To investigate the responses of soybean seed against FM stress and identify the underlying biochemical pathways involved, a greenhouse was equipped with an artificial rain producing system to allow the induction of mold growth on soybean seed. The induced quality changes and stress responses were revealed on the levels of both transcriptome and metabolome. The results showed that soybean seeds produced under FM stress conditions had an abnormal and inferior appearance, and also contained less storage reserves, such as protein and polysaccharide. Transcriptional analysis demonstrated that genes involved in amino acid metabolism, glycolysis, tricarboxylic acid, β-oxidation of fatty acids, and isoflavone biosynthesis were induced by FM stress. These results were supported by a multiple metabolic analysis which exhibited increases in the concentrations of a variety of amino acids, sugars, organic acids, and isoflavones, as well as reductions of several fatty acids. Reprogramming of these metabolic pathways mobilized and consumed stored protein, sugar and fatty acid reserves in the soybean seed in order to meet the energy and substrate demand on the defense system, but led to deterioration of seed quality. In general, FM stress induced catabolism of storage reserves and diminished the quality of soybean seed in the field. This study provides a more profound insight into seed deterioration caused by FM stress.

关键词： seed deterioration seed quality primary metabolism storage reserves resistance

来源：

维普期刊数据库

同方期刊数据库评论