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circ_0000144靶向miR-502-5p调控人视网膜母细胞瘤Y79细胞增殖和凋亡及迁移侵袭

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国际眼科杂志 2021年第10期21卷 1686-1692页

作者：储明慧陈海银张晓力中国江苏省海安市中医院眼耳鼻喉科 226600

目的:探讨环状RNA(circRNA)circ_0000144是否靶向微小RNA(miRNA)-502-5p调控人视网膜母细胞瘤Y79细胞增殖、凋亡、迁移侵袭。方法:将Y79细胞分为si-NC组(转染si-NC)、si-circ_0000144组(转染si-circ_0000144)、miR-NC组(转染miR-NC)、miR-502-5p组(转染miR-502-5p mimic)、pcDNA组(转染pcDNA)、pcDNA-circ_0000144组(转染pcDNA-circ_0000144)、si-circ_0000144+anti-miR-NC组(转染si-circ_0000144+anti-miR-NC)、si-circ_0000144+anti-miR-502-5p组(转染si-circ_0000144+anti-miR-502-5p)。实时定量聚合酶链反应(qRT-PCR)检测视网膜母细胞瘤组织和细胞中circ_0000144和miR-502-5p表达,溴化噻唑蓝四氮唑(MTT)检测细胞增殖,免疫印迹实验(western blot)测定细胞核相关抗原Ki-67(Ki-67)、B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、基质金属蛋白酶(MMP)-2、MMP-9蛋白表达,流式细胞术分析细胞凋亡,Transwell测定细胞迁移、侵袭。生物信息学预测与双荧光素酶报告实验分析circ_0000144是否靶向miR-502-5p。结果:31例视网膜母细胞瘤组织中circ_0000144表达量比瘤旁组织高,miR-502-5p表达量比瘤旁组织低(P<0.05)。与si-NC组比较,si-circ_0000144组Y79细胞中circ_0000144表达量、OD值、Ki-67、Bcl-2、MMP-2、MMP-9蛋白表达量、迁移、侵袭细胞数减少,Bax蛋白表达量、凋亡率增加(P<0.05)。circ_0000144靶向负调控miR-502-5p的表达。miR-502-5p组较miR-NC组增加Y79细胞凋亡率、Bax蛋白表达量,减少OD值、迁移及侵袭细胞数、Ki-67、Bcl-2、MMP-2、MMP-9蛋白表达量(P<0.05)。与si-circ_0000144+anti-miR-NC组比较,si-circ_0000144+anti-miR-502-5p组Y79细胞凋亡率、Bax蛋白表达量降低,OD值、迁移及侵袭细胞数、Ki-67、Bcl-2、MMP-2、MMP-9蛋白表达量升高。结论:视网膜母细胞瘤组织中circ_0000144高表达,通过负调控miR-502-5p抑制其表达降低视网膜母细胞瘤Y79细胞增殖、迁移侵袭,促进凋亡。

关键词：视网膜母细胞瘤 circ_0000144 miR-502-5p 增殖凋亡迁移侵袭

基于FFT的一种计及负频率影响的相位差测量新方法

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计量学报 2008年第2期29卷 168-171页

作者：张海涛涂亚庆后勤工程学院信息工程系重庆400016

用FFT法测量低频正弦信号相位差时,精度明显下降,甚至无法测量,其主要原因之一是模型中忽略了负频率成分的贡献。基于FFT法,提出了一种计及负频率影响的相位差测量方法,分别给出了加矩形窗和加汉宁窗对应的相位差计算公式。仿真结果表明... 详细信息

用FFT法测量低频正弦信号相位差时,精度明显下降,甚至无法测量,其主要原因之一是模型中忽略了负频率成分的贡献。基于FFT法,提出了一种计及负频率影响的相位差测量方法,分别给出了加矩形窗和加汉宁窗对应的相位差计算公式。仿真结果表明,在无噪声背景下,具有很高的精度,尤其是加汉宁窗后,误差接近双精度运算的下限;在噪声背景下,该方法的测量精度也高于FFT法。提出的测量方案简单实用,特别适用于频率很低或接近奈奎斯特频率的正弦信号相位差测量。

关键词：计量学相位差测量快速傅里叶变换负频率低频信号离散傅里叶变换

Antithrombotic and antiplatelet therapies in relation to risk stratification in patients with non-ST elevation acute coronary syndrome： insights from the Sino-Global Registry of Acute Coronary Events

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Chinese Medical Journal 2009年第5期122卷 502-508页

作者： zhang Li-jie CHEN Yun-dai SONG Xian-tao ZHAO Fu-hai Lü Shu-zheng Department of Cardiology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Department of Cardiology Chinese People's Liberation Army General Hospital Beijing 100853 China Department of Cardiology Xiyuan Hospital of China Academy of Chinese Medical Science Beijing 100091 China

Background Antithrombotic and antiplatelet therapies have been proposed to treat non-ST elevation acute coronary syndrome （NSTEACS）, yet limited information is available about their applications from a multicenter ＂real-world＂ clinical procedure, especially in China. This study was undertaken to characterize the use of antithrombotic and antiplatelet agents in relation to the risk levels of the NSTEACS patients who were enrolled in Sino-Global Registry of Acute Coronary Events （GRACEs） registry study. Methods We analyzed the data from 618 Chinese NSTEACS patients stratified into low-（n=151）, intermediate-（n=233）, and high-risk groups （n=-234） based on GRACE risk scores. The baseline characteristics, clinical presentations, antithrombotic and antiplatelet agents were recorded and compared among the three groups. Results The administration rates of low-molecular-weight heparins （LMWHs）（86.08%） and thienopyridines （85.92%） were higher whereas the administration rate of glycoprotein Ⅱb/Ⅲa inhibitor （1.78%） was much lower than those reported previously. Meanwhile, within the first 24 hours of admission, the use of heparin/LMWHs in the high-risk group was more than that in the intermediate- and low-risk groups （73.50% vs 63.09% vs 55.63%, P=0.001）. Furthermore, the combination of antithrombotic and antiplatelet medications showed no significant differences in all groups. Conclusions In the ＂real world＂ practice of China, the antithrombotic and antiplatelet therapies on NSTEACS are well adherent to the current guidelines except for several gaps, such as the very low use of glycoprotein Ⅱb/Ⅲa inhibitor. Moreover, these antithrombotic and antiplatelet treatments usually tend to be underused for the high-risk ones.

关键词： non-ST elevation acute coronary syndromes risk stratification antithrombotic antiplatelet

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基于品位的成本分摊方法及其应用

工程热物理学报 2016年第6期37卷 1141-1146页

作者：亓海青韩巍张娜中国科学院工程热物理研究所北京100190 中国科学院大学北京100190

成本分摊方法是热经济学建模的重要组成部分,现有的成本分摊方法较少考虑能量品质变化对能量单位成本的影响,不符合优质优价的原则。本文通过分析能量利用过程中品位变化,建立了单位成本与值的函数关系,提出一种新的成本分摊方法。利... 详细信息

成本分摊方法是热经济学建模的重要组成部分,现有的成本分摊方法较少考虑能量品质变化对能量单位成本的影响,不符合优质优价的原则。本文通过分析能量利用过程中品位变化,建立了单位成本与值的函数关系,提出一种新的成本分摊方法。利用该方法对热电联产系统成本计算,并与常规分摊方法对比,热的单位成本由原来的2.66kW/kW降低到1.76 kW/kW,电的单位成本由原来的1.79 kW/kW升高到2.27 kW/kW,新方法体现了优质优价的原则,更加真实反映了热电成本。此外,在新方法基础上研究了主要设计参数对热电成本的影响,提高压气机及透平效率均可降低热电成本。该方法可用于多联产系统的成本计算和性能评价。

关键词： [火用]经济学品位成本分摊 成本热电联产

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埕北断坡区断层输导体系与油气成藏模式

新疆石油地质 2007年第6期28卷 721-724页

作者：高长海查明张新征中国石油大学(华东)地球资源与信息学院山东东营257061 中国石化胜利油田有限责任公司地质录井公司山东东营257064

在系统描述埕北断坡区断裂体系及分布特征的基础上,着重分析了断层的封堵和输导作用及其对研究区油气分布的控制作用;提出了研究区断层输导的3种模式,即垂向输导模式、侧向输导模式及阶梯状输导模式,并总结了不同断层输导作用形成的3种... 详细信息

在系统描述埕北断坡区断裂体系及分布特征的基础上,着重分析了断层的封堵和输导作用及其对研究区油气分布的控制作用;提出了研究区断层输导的3种模式,即垂向输导模式、侧向输导模式及阶梯状输导模式,并总结了不同断层输导作用形成的3种不同的成藏模式及特点。

关键词：埕北断坡区断层封堵断层输导成藏模式

Diagnostic accuracy of various glomerular filtration rates estimating equations in patients with chronic kidney disease and diabetes

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Chinese Medical Journal 2010年第6期123卷 745-751页

作者： LI hai-xia XU Guo-bin WANG Xue-jing zhang Xu-chu YANG Jian-mei Department of Clinical Laboratory Peking University First HospitalBeijing 100034China Department of Nuclear Medicine Peking University First HospitalBeijing 100034China Department of Endocrinology Peking University First HospitalBeijing 100034China

Background The equations for estimating glomerular filtration rate （GFR） based on creatinine have been found to have limitations and have not been generalizable across all populations. Equations based on cystatin C provide an alternative method to estimate GFR. Whether the equation based on cystatin C alone or combined creatinine would improve GFR estimates has not been validated among Chinese patients with chronic kidney disease （CKD） and diabetes. The aim of this study was to compare the performance of the modification of diet in renal disease （MDRD） equation based on creatinine with the five cystatin C-based formulae for estimation of GFR in patients with CKD and diabetes. Methods A total of 166 patients with CKD and 91 patients with type 2 diabetes were enrolled in this study. Cystatin C was measured by using the particle-enhanced immunonephelometric method and estimated formulae proposed by five different investigator teams （Stevens, Ma, Rule, Macisaac and Perkins）. The plasma clearance of ^99mTc-DTPA was determined as measured GFR （mGFR）.Results For CKD patients, the bias and accuracy for the Ma and Macisaac equations were superior compared with the MDRD, and the mean results for the Ma formula were closer to mGFR than the other equations in CKD stages 2-5. The differences between Macisaac and mGFR in CKD stages 2-4 were significantly less than those in CKD stage 1 or *** and Rule＇s formulae revealed a similar bias and accuracy compared with the MDRD equation. The MDRD formula had a higher accuracy in CKD stages 3-5 as compared with the results in other stages. For diabetic patients, the mean results between Macisaac and mGFR were closer than those of other equations in mGFR≥90 ml·min^-1·1.73 m^-2 stage, in GFR 60-89 ml·min^-1·1.73 m^-2 stage, the MDRD formula showed the smallest difference compared with other equations. All equations overestimated GFR in the cases with GFR 〈60 ml·min^-1·1.73 m^-2 stages. The MDRD formula had a greater accuracy within 50

关键词： creatinine cystatin C kidney diseases diabetes mellitus, type 2 glomerular filtration rate

Long-term efficacy,safety and biocompatibility of a novel sirolimus eluting iron bioresorbable scaffold in a porcine model

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Bioactive Materials 2024年第9期39卷 135-146页

作者： Ya-Nan Gao Hong-Tao Yang Zi-Feng Qiu Feng Qi Qian-Hong Lu Jian-Feng Zheng Zi-Wei Xi Xin Wang Li Li Gui zhang De-Yuan zhang Yu-Die Lu hai-Ping Qi Hong Qiu Run-Lin Gao Yu-Feng Zheng Department of Cardio-Metabolic Medicine Center Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Department of Cardiology Coronary Artery Disease CenterFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials Animal Experimental CenterFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Department of Pathology Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina School of Engineering Medicine Beihang UniversityBeijingChina R&D Center Biotyx Medical(Shenzhen)Co.Ltd.ShenzhenChina Department of Cardiology Fuwai Yunnan HospitalChinese Academy of Medical SciencesAffiliated Cardiovascular Hospital of Kunming Medical UniversityKunmingChina Peking University Health Science Center BeijingChina Department of Cardiology Beijing Tsinghua Changgung HospitalBeijingChina Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing Institute of Heart Lung and Blood Vessel DiseasesBeijingChina School of Materials Science and Engineering Peking UniversityBeijingChina

Iron is considered as an attractive alternative material for bioresorbable scaffolds(BRS).The sirolimus eluting iron bioresorbable scaffold(IBS),developed by Biotyx Medical(Shenzhen,China),is the only iron-based BRS with an ultrathin-wall *** study aims to investigate the long-term efficacy,safety,biocompatibility,and lumen changes during the biodegradation process of the IBS in a porcine model.A total of 90 IBSs and 70 cobaltchromium everolimus eluting stents(EES)were randomly implanted into nonatherosclerotic coronary artery of healthy mini *** multimodality assessments including coronary angiography,optical coherence tomography,micro-computed tomography,magnetic resonance imaging,real-time polymerase chain reaction(PCR),and histopathological evaluations,were performed at different time *** was no statistical difference in area stenosis between IBS group and EES group at 6 months,1year,2 years and 5 *** the scaffolded vessels narrowed at 9 months,expansive remodeling with increased mean lumen area was found at 3 and 5 *** IBS struts remained intact at 6 months,and the corrosion was detectable at 9 *** 5 years,the iron struts were completely degraded and absorbed in situ,without in-scaffold restenosis or thrombosis,lumen collapse,aneurysm formation,and chronic *** local or systemic toxicity and abnormal histopathologic manifestation were found in all *** from real-time PCR indicated that no sign of iron overload was reported in scaffolded ***,the IBS shows comparable efficacy,safety,and biocompatibility with EES,and late lumen enlargement is considered as a unique feature in the IBS-implanted vessels.

关键词： Bioresorbable scaffold Sirolimus eluting iron bioresorbable scaffold Preclinical study Completely bioresorbable Late lumen enlargement

Subchondral bone grafting reduces degenerative change of knee joint in patients of giant cell tumor of bone

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Chinese Medical Journal 2013年第16期126卷 3053-3056页

作者： XU hai-rong NIU Xiao-hui zhang Qing HAO Lin DING Yi LI Yuan Department of Orthopedic Oncology Surgery Beijing JishuitanHospital Peking University Beijing 100035 China

Background Giant cell tumors （GCTs） most commonly occur around the *** most beneficial procedure for this disease has been extensive curettage with ***,since many GCTs may compromise the subchondral bone,surgery can further jeopardize the articular cartilage and result in secondary *** this study,we aimed to determine the factors associated with the development of degenerative arthritis and the effect of bone grafting on the prevention of secondary *** We retrospectively analyzed 76 patients with GCT around the *** mean age at first diagnosis was 31.1 *** treatments included extensive curettage and cementation with or without bone grafting in the subchondral *** follow-up was a median duration of 35 months,ranging from 18 to 113 *** The local recurrence rate was 5.3％（4/76）.Secondary degenerative changes occurred in 30.3％（23/76） of the *** than 10 mm of the residual thickness of the remaining subchondral bone was correlated with secondary degenerative changes in 57 patients （P ＜0.001）.Of these 57 patients,56.5％（13/23） treated with bone cement reconstruction alone developed secondary degenerative changes; following bone grafting,the rate decreased to 29.4％（10/34）,with a statistically significant difference （P=0.041）.Conclusions GCT patients with less residual thickness of the subchondral bone are more likely to develop degenerative arthritis after *** grafting in the subchondral bone area is recommended when the residual thickness of the subchondral bone is less than 10 mm.

关键词： giant cell tumor bone grafting secondary osteoarthritis knee

First records of Triatoma rubrofasciata(De Geer,1773)(Hemiptera,Reduviidae)in Foshan,Guangdong Province,Southern China

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Infectious Diseases of Poverty 2017年第1期6卷 1143-1148页

作者： Qin Liu Yun-hai Guo Yi zhang Zheng-Bin Zhou Liang-Liang zhang Dan Zhu Xiao-Nong Zhou National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionKey Laboratory of Parasite and Vector BiologyMinistry of HealthWHO Collaborating Center for Tropical DiseasesShanghai 200025People’s Republic of China

Background:Triatomines,also known as kissing bugs,which are found throughout the world and especially in Latin America,are well known natural vectors that transmit American trypanosomiasis,also called Chagas *** China,the presence of two species of Triatoma(Triatoma rubrofasciata and ***)was recorded in the *** to the growing population and the increasing risk of the global spread of Chagas disease,triatomines became a potential public health nuisance,and in 2016,we started monitoring triatomine activities in southern ***:Triatomine specimens were collected by the National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,and identified by their morphological characteristics under a dissecting *** addition to morphological analysis,the genomic DNA of the specimens was extracted,and the mitochondrial 16S rRNA,the cytochrome b(CytB)gene and the nuclear ribosomal 28S rRNA gene were PCRamplified to analyze and confirm the species ***:One female adult insect and one male adult insect were collected in a dwelling in the rural area of Shunde County,Foshan City,Guangdong Province,China(22°42′44.63″N,113°08′45.34″E).The results from the morphological and genetic analyses indicated that these triatomines were ***:This is the first time that the occurrence of *** has been confirmed in Foshan City,Guangdong Province in southern *** studies are needed to reach a clearer understanding of the ecology of this species of triatomine,since it has been found to be naturally infected by Trypanosoma cruzi and *** and there is evidence of its domiciliation capabilities.

关键词： Triatominae Triatoma rubrofasciata Foshan Southern China