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Journal of Mountain Science 2014年第4期11卷 1001-1014页

作者： CHANG Ming TANG Chuan zhang dan-dan MA Guo-chao State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology

The Longchi area with the city of Dujiangyan, in the Sichuan province of China, is composed of Permian stone and diorites and Triassic sandstones and mudstones intercalated with slates. An abundance of loose co-seismic materials were present on the slopes after the May 12, 2008 Wenchuan earthquake, which in later years served as source material for rainfall-induced debris flows or shallow landslides. A total of 48 debris flows, all triggered by heavy rainfall on 13th August 20l0, are described in this paper. Field investigation, supported by remote sensing image interpretation, was conducted to interpret the co-seismic landslides in the debris flow gullies. Specific characteristics of the study area such as slope, aspect, elevation, channel gradient, lithology, and gully density were selected for the evaluation of debris flow susceptibility. A score was given to all the debris flow gullies based on the probability of debris flow occurrence for the selected factors. In order to get the contribution of the different factors, principal component analyses were applied. A comprehensive score was obtained for the 48 debris flow gullies which enabled us to make a susceptibility map for debris flows with three classes. Twenty-two gullies have a high susceptibility, twenty gullies show a moderate susceptibility and six gullies have a low susceptibility for debris flows.

关键词： Debris flow Landslide Probability Susceptibility assessment Grey correlation

Metabonomics delineates allergic reactions induced by Shuang-huang-lian injection in rats using ultra performance liquid chromatography-mass spectrometry

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Chinese Journal of Natural Medicines 2018年第8期16卷 628-640页

作者： GU Yuan-Yuan SHI Lang zhang dan-dan HUANG Xin CHEN Da-Zhong Research Institute of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine First Clinical Medical College Heilongjiang University of Chinese Medicine

Shuang-huang-lian Injection(SHLI) is the first successfully developed drug from traditional Chinese medicine(TCM) powder for injection,since its use for the treatment of acute respiratory tract infection,pneumonia,influenza,*** the same time,its allergic reactions have also emerged,which limits clinical ***,few scholars pay attention to the mechanism of allergic *** this present study,metabonomics technology was used to explore the changes in endogenous metabolites in urine of the rat model of SHLI induced allergic reaction;we and analyzed the metabolites,metabolic pathway,and the mechanism which were closely related to the allergic *** levels of serum histamine and tryptase were examined and changes in histomorphology were also *** on the UPLC-Q-TOF/MS metabonomics,we carried out the pattern recognition analysis,selected potential biomarkers associated with allergic reactions,and explored the pathological mechanism for SHLI induced allergic reaction,which laid the foundation for the safety research of *** results showed that SHLI increased the levels of serum histamine and tryptase in rats with allergic reaction;we determined 15 biomarkers in rat allergic reaction model induced by SHLI and found multiple metabolic pathways involved,such as metabolism of linolenic acid,phenylalanine,amino acid,2-oxo acid,and purine and other metabolic pathways.

关键词： Shuang-huang-lian injection Biomarker Metabonomics Allergic mechanism Metabolites

Cold hardiness of the invasive fall armyworm,Spodoptera frugiperda in China

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Journal of Integrative Agriculture 2021年第3期20卷 764-771页

作者： zhang dan-dan ZHAO Sheng-yuan WU Qiu-lin LI Yu-yan WU Kong-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Fall armyworm,Spodoptera frugiperda(***,1797),a crop pest native to tropical and subtropical regions of America,has invaded and spread into most regions in China,posing a severe threat to China’s *** cold hardiness directly determines its geographic distribution through adapting to winter temperatures of different ***,we measured supercooling points and lethal time(LT)at low temperatures of *** supercooling points for developmental stages in increasing order were:adults(-15.05℃)

关键词： Spodoptera frugiperda supercooling point overwinter China

Insecticide resistance monitoring for the invasive populations of fall armyworm,Spodoptera frugiperda in China

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Journal of Integrative Agriculture 2021年第3期20卷 783-791页

作者： zhang dan-dan XIAO Yu-tao XU Peng-jun YANG Xian-ming WU Qiu-Iin WU Kong-ming Sfafe Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Agricultural Genomics institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120P.R.China Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdao 266101P.R.China

Fall armyworm has invaded China and colonized its populations in tropical and sub-tropical regions of South China since December *** spray has been widely used to control the pest,which shall lead to resistance *** this research,we collected five populations of the pest from Yunnan,Hainan,Tibet,and Fujian of China,and tested their susceptibilities to pyrethroid,organophosphorus,oxadiazine,diamide,antibiotics and other types of insecticides(14 insecticides totally)in the *** on the susceptible baseline published from the previous studies,the resistance ratio was 615-1068-fold to chlorpyrifos,60-388-fold to spinosad,26-317-fold to lambda-cyhalothrin,13-29-fold to malathion,9-33-fold to fenvalerate,8-20-fold to deltamethrin,3-8-fold to emamectin benzoate and 1-2-fold to chlorantraniliprole,*** median lethal concentration(LC_(50))of other six insecticides without the susceptible baselines was 148.27-220.96μgmL^(-1) for beta-cypermethrin,87.03-128.43μgmL^(-1) for chlorfenapyr,16.35-99.67μgmL^(-1) for indoxacarb,10.55-51.01μgmL^(-1) for phoxim,7.08-8.78μgmL^(-1) for M-EBI(the mixed insecticide of emamectin benzoate and indoxcarb)and 1.49-4.64μg mL^(-1) for *** study can be helpful for chemical control as well as for resistance monitoring and management of the pest in China.

关键词： Spodoptera frugiperda insecticides resistance monitoring China

Extracellular superoxide dismutase VdSOD5 is required for virulence in Verticillium dahliae

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Journal of Integrative Agriculture 2021年第7期20卷 1858-1870页

作者： TIAN Li HUANG Cai-min zhang dan-dan LI Ran CHEN Jie-yin SUN Wei-xia QIU Nian-wei DAI Xiao-feng College of Life Science Qufu Normal UniversityQufu 273165P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests/lnstitute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

Plants produce reactive oxygen species(ROS) to defend pathogens. To counteract this attack, certain pathogens express superoxide dismutases(SODs) to scavenge host-derived ROS. However, the roles of SODs in Verticillium dahliae, an important vascular pathogen, are not clear. Our previous study has shown that a putative extracellular SOD(VdSOD5) of V. dahliae is significantly induced by culturing in cotton tissues, suggesting that VdSOD5 may play an important role in host–pathogen interactions and virulence. Here, we showed that VdSOD5 encoded a superoxide dismutase with a cofactor copper-binding site and a functional signal peptide that can conduct protein secretion in an invertase-mutated yeast strain. The mutations in VdSOD5(ΔVdSOD5) did not change the normal vegetative growth and conidial production but reduced the virulence of V. dahliae on susceptible host cotton. Further studies showed that the transcription of Vd SOD5 was significantly up-regulated during the early stage of infection, and the loss-of-function of VdSOD5 decreased culture filtrate and fungal tissue SOD activities of V. dahliae by 74 and 28%, respectively. Compared to the wild-type strain Vd991, the ΔVdSOD5 showed the same sensitivity to the intracellular ROS generator menadione. Furthermore, nitroblue tetrazolium(NBT) staining demonstrated that VdSOD5 functioned in the detoxification of superoxides generated by host roots during infection. These results suggest that VdSOD5 of V. dahliae is an important virulence factor, secreted out of cells to combat host-derived ROS.

关键词： Verticillium dahliae superoxide dismutase secretion virulence ROS detoxification

Effect of Aluminum and Titanium Treatment on Non-Metallic Inclusions and Microstructures of CGHAZ in HSLA Steel

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Journal of Iron and Steel Research International 2011年第S1期18卷 360-365页

作者： CHAI Feng,YANG Cai-fu,SU Hang,zhang Yong-quan,zhang dan-dan (China Iron and Steel Research Institute,Beijing 100081,China) China Iron & Steel Res Inst Beijing 100081 Peoples R China

The Effect of A1 and Ti treatment on non-metallic inclusions and microstructures of coarse-grain HAZ in HSLA stee1 was investigated in this paper based on experiments and thermodynamic *** results showed that the inclusions in A1 treated steel were mainly aluminum oxides and titanium nitrides which could not promote the formation of acicular ferrite *** of coarse-grain HAZ in A1 treated steels consists of heavy grain boundary ferrite and ferrite side *** inclusions in Ti treated steel were A1,Ti,Mg,Ca composite oxides with size in the range of 0.5-3μm and titanium nitrides with size less than 0.3μ*** composite oxide could promote the formation of acicular ferrite and microstructures of coarse-grain HAZ in Ti treated steel consists of grain boundary ferrite,small amounts of ferrite side plate and large amounts of intragranular acicular *** size of grain boundaries ferrite was increased and the amount of ferrite side plate was decreased with the increase of soaking time at the peak *** amount of grain boundary ferrite and the size of acicular ferrite were also increased with the increase of cooling rate during ferrite phase formation.

关键词： Ti treatment A1 treatment coarse grain HAZ grain boundary ferrite acicular ferrite

Xinglou Chengqi Decoction improves neurological function in experimental stroke mice as evidenced by gut microbiota analysis and network pharmacology

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Chinese Journal of Natural Medicines 2021年第12期19卷 881-899页

作者： GAO Qiang HAN Zhen-Yun TIAN dan-Feng LIU Gan-Lu WANG Zhen-Yi LIN Jing-Feng CHANG Ze zhang dan-dan XIE Ying-Zhen SUN Yi-Kun YAO Xing-Wei MA Da-Yong Beijing University of Chinese Medicine Beijing 100029China Shenzhen Hospital of Beijing University of Chinese Medicine(Longgang) Shenzhen 518172China Department of Neurology Dongzhimen Hospital Affiliated to Beijing University of Chinese MedicineBeijing 100700China Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing Dongzhimen Hospital Affiliated to Beijing University of Chinese MedicineBeijing 100700China Clinical Laboratory Dongzhimen Hospital Affiliated to Beijing University of Chinese MedicineBeijing 100700China

The current study was designed to explore the brain protection mechanism of Xinglou Chengqi Decoction(XCD)based on gut microbiota analysis and network pharmacology. A transient middle cerebral artery occlusion(MCAO) model of mice was established, followed by behavioral evaluation, TTC and TUNEL staining. Additionally, to investigate the effects of gut microbiota on neurological function after stroke, C57BL/6 mice were treated with anti-biotic cocktails 14 days prior to ischemic stroke(IS) to deplete the gut microbiota. High-throughput 16S rDNA gene sequencing, metabonomics technique, and flow multifactor technology were used to analyze bacterial communities, SCFAs and inflammatory cytokines respectively. Finally, as a supplement, network pharmacology and molecular docking were applied to fully explore the multicomponent-multitarget-multichannel mechanism of XCD in treating IS, implicated in ADME screening, target identification, network analysis, functional annotation, and pathway enrichment *** found that XCD effectively improved neurological function, relieved cerebral infarction and decreased the neuronal ***, XCD promoted the release of anti-inflammatory factor like IL-10, while down-regulating pro-inflammatory factors such as TNF-α, IL-17A, and IL-22. Furthermore, XCD significantly increased the levels of short chain fatty acids(SCFAs), especially butyric acid. The mechanism might be related to the regulation of SCFAs-producing bacteria like Verrucomicrobia and Akkermansia, and bacteria that regulate inflammation like Paraprevotella, Roseburia, Streptophyta and Enterococcu. Finally, in the network pharmacological analysis, 51 active compounds in XCD and 44 intersection targets of IS and XCD were selected. As a validation, components in XCD docked well with key targets. It was obviously that biological processes were mainly involved in the regulation of apoptotic process, inflammatory response, response to fatty acid, and regulation of est

关键词： Xinglou Chengqi Decoction Ischemic stroke Gut microbiota Network pharmacology

Population occurrence of the fall armyworm,Spodoptera frugiperda(Lepidoptera:Noctuidae),in the winter season of China

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Journal of Integrative Agriculture 2021年第3期20卷 772-782页

作者： YANG Xian-ming SONG Yi-fei SUN Xiao-xu SHEN Xiu-jing WU Qiu-lin zhang Hao-wen zhang dan-dan ZHAO Sheng-yuan LIANG Ge-mei WU Kong-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China College of Plant Protection Southwest UniversityChongqing 400716P.R.China

Fall armyworm(FAW),Spodoptera frugiperda(***),has become an important pest in Chinese agricultural systems since its invasion on 11 December *** its establishment,FAW months in the year-round breeding region have become the main source population migrating to other areas in *** investigations were conducted in tropical and subtropical regions to improve understanding of its year-round breeding area in *** results showed that FAW larval density was significantly correlated with the seasonal temperature of the location *** FAW larvae maintained a high density in the tropical area and were frequently found in sites of the south subtropical region,but were absent from the north subtropical region and the northern part of the central subtropical *** results indicated that FAW can reproduce annually in the tropical and south subtropical regions of China,including Hainan,Taiwan and the southern area of Fujian,Guangdong,Guangxi,Guizhou,and ***,great effort should be made to monitor and control FAW in the year-round breeding region to suppress the population density of this area and to reduce migration of moths into northern parts of *** study clarifies the occurrence area of the pest in winter in China and provides much valuable information for its population forecasting and management.

关键词： fall armyworm temperature year-round breeding region tropical region subtropical region

Analysis of phototactic responses in Spodoptera frugiperda using Helicoverpa armigera as control

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Journal of Integrative Agriculture 2021年第3期20卷 821-828页

作者： LIU Ying-jie zhang dan-dan YANG Li-yu DONG Yong-hao LIANG Ge-mei Philip DONKERSLEY REN Guang-wei XU Peng-jun WU Kong-ming Key Laboratory of Tobacco Pest Monitoring Controlling&Integrated Management Tobacco Research InstituteChinese Academy of Agricultural SciencesQingdao 266101P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Lancaster Environment Centre Lancaster UniversityLancasterLA14YQUK

Light traps are widely utilized to monitor and manage insect pest *** late 2018,the fall armyworm(FAW),Spodoptera frugiperda,invaded China through Yunnan Province representing a huge threat to grain *** estimate the efficiency of light traps on FAW moths,we first identified the opsin genes from FAW by using the *** analysis indicated that the four opsins of FAW were clustered with those of other Noctuidae *** expressed levels of opsins in *** were lower than in Helicoverpa armigera,suggesting a different phototactic response between the two ***,we determined the phototactic behavior of FAW using *** as a control,which is widely monitored and managed using light traps in *** results indicated that the two moths species showed significantly different phototactic behavior and both female and male FAW displayed faster flight-to-light speed than *** may be due to a faster flight capacity in FAW compared to ***,the capture rate of both female and male of *** was significantly lower than that of ***,which was consistent with the expression levels of *** results support the positive phototaxis of *** moths and suggest light traps could be used for monitoring and managing the pests,but with a lower efficiency than ***.

关键词： Spodoptera frugiperda Helicoverpa armigera light performance opsin genes light trap

Synthesis,Characterization and Catalytic Activity of Salen Co(Ⅲ)Cl in Alternating Copolymerization of CO2 and Propylene Oxide

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Chinese Journal of Structural Chemistry 2020年第1期39卷 86-95,3页

作者： YU Jin-Fa MAO Min-Jie LI Hui-Ping ZHOU Xian-Bo zhang dan-dan CHEN Jian-Xin zhang Zhi-Chun College of Chemistry and Materials Science Fujian Normal UniversityFuzhou 350007China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

The title complex Salen Co(Ⅲ)Cl(Salen = 6,6’-((1E,1’E)-(cyclohexane-1,2-diylbis(azaneylylidene))bis(methaneylylidene))bis(2,4-di-tert-butylphenol)) was synthesized and characterized by elemental analysis, IR spectroscopy, 1H NMR and UV-Vis. The complex can be used as catalyst for the propylene oxide(PO)/CO2 copolymerization in different conditions of reaction time, reaction temperature, carbon dioxide pressure and monomer concentration, and the optimum conditions for copolymerization were obtained.

关键词： cobalt(Ⅲ)complex salen ploy(propylene carbonate) catalytic activity