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Direct generation of an ultrafast vortex beam in a CVD-graphene-based passively mode-locked Pr:LiYF4 visible laser

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Photonics Research 2019年第11期7卷 1209-1213页

作者： Nan Li Junjie Huang Bin Xu Yaqi Cai Jie Lu linjie zhan Zhengqian Luo Huiying Xu Zhiping Cai Weiwei Cai Department of Electronic Engineering Xiamen UniversityXiamen 361005China Shenzhen Research Institute of Xiamen University Shenzhen 518057China Department of Physics State Key Laboratory of Physical Chemistry of Solid SurfacesXiamen UniversityXiamen 361005China

We report on the direct generation of passively mode-locked vortex lasers in the visible spectral region, for the first time to the best of our knowledge, using a Pr :Li YF4(Pr:YLF) crystal as the gain medium. A stable mode-locked TEM00 mode has been achieved with a maximum average output power of 75 m W using a graphene saturable absorber mirror. The mode-locked pulse width is measured to be as short as about 73.4 ps at a repetition rate of about 140 MHz, and the laser wavelength is at about 721 nm with spectral width of about 0.5 nm. By slightly misaligning the laser resonator, a first-order Laguerre-Gaussian mode(LG0,1) has also been obtained with output power reduced to about 22 m W. The achieved LG0,1 mode has been verified via a home made improved Fizeau interferometer. This work provides a simple and universal method for direct generation of an ultrafast vortex laser,which can be readily extended to other spectral regions by using different laser gain mediums.

关键词： passive interferometer resonator

大豆浅圆仓中嗜虫书虱种群发生动态及其检测方法研究

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中国粮油学报 2024年第7期39卷 6-11页

作者：吴宗霖叶天伟郭晓勇李林杰张慧民徐展张茹王争艳中央储备粮新郑直属库有限公司新郑451100 河南工业大学粮食和物资储备学院郑州450001

在害虫综合治理中,害虫种群监测可以为害虫防治时机的选择提供依据。本文在大豆浅圆仓内,利用探管诱捕器、圆盘诱捕器、瓦楞纸板诱捕器和取样筛检法检测书虱的发生动态,分析影响害虫发生动态的因素,并比较不同检测方法的检测效果。研究... 详细信息

在害虫综合治理中,害虫种群监测可以为害虫防治时机的选择提供依据。本文在大豆浅圆仓内,利用探管诱捕器、圆盘诱捕器、瓦楞纸板诱捕器和取样筛检法检测书虱的发生动态,分析影响害虫发生动态的因素,并比较不同检测方法的检测效果。研究结果表明,嗜虫书虱主要分布在靠墙的位置,中心部位分布的较少;与温度相比,仓房湿度对嗜虫书虱发生密度的影响更为显著;在3种诱捕器中,探管诱捕器的诱捕效率最高,能较早地检测到低密度的书虱。

关键词：浅圆仓嗜虫书虱取样筛检法诱捕器

Upregulation of mtSSB by interleukin-6 promotes cell growth through mitochondrial biogenesis-mediated telomere elongation in colorectal cancer

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Upregulation of mtSSB by interleukin-6 promotes cell growth ...

基于全局敏感性分析和PE-HMGE的轴承润滑特性优化

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Gang Wang Yibing Chen Qian Wang Xiacheng Sun linjie He Lei zhan Xu Guo Qichao Huang Jinliang Xing 第四军医大学唐都医院普通外科

Objective:It is now widely accepted that mitochondrial biogenesis is inhibited in most cancer cells. Interestingly, one of the possible exceptions is colorectal cancer(CRC), in which the content of mitochondria has been found to be higher than in normal colon mucosa. However, to date,the causes and effects of this phenomenon are still unclear. Methods : In the present study, we systematically investigated the functional role of mitochondrial single-strand DNA binding protein(mtSSB), a key molecule in the regulation of mitochondrial DNA(mtDNA) replication, in the mitochondrial biogenesis and CRC cell growth. First, we determined the expression of mtSSB in paired CRC tissues and investigated the clinical relevance of mtSSB expression. Then the effect of mtSSB on in vitro and in vivo cell proliferation was assessed. We next investigated the effects of mtSSB on mitochondrial biogenesis and functions in CRC cells. Furthermore,we investigated the effects of mtSSB on telomerase activity and telomere elongation. At last, we investigated the function and mechanism of the regulation of proinflammatory cytokines IL-6 on the expression of mtSSB in ***:Our results demonstrated that mtSSB was frequently upregulated in CRC tissues and found that CRC patients with high mtSSB expression had a significantly poorer overall survival and recurrence-free survival than those with low mtSSB expression(Log rank P = 0.025 and 0.006,respectively). We also found that forced expression of mtSSB significantly enhanced the proliferation of CRC cells, while knockdown of mtSSB suppressed the proliferation of CRC cell lines. In addition,xenograft nude mice mode showed that CRC cells with stable mtSSB overexpression exhibited a significant increase in tumorigenesis capacity in vivo, whereas CRC cells with stable mtSSB knockdown exhibited decreased tumorigenesis capacity. Mechanistically, the results showed that overexpression of mtSSB in CRC cells resulted in increased mtDNA content, the coun

关键词： mtSSB mitochondrial biogenesis telomere elongation interleukin 6 reactive oxygen species colorectal cancer

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汽车工程 2018年第12期40卷 1488-1493,1487页

作者：李林洁韦静思何志良陈宇占文锋广州汽车集团有限公司汽车工程研究院广州511434

针对复杂非线性系统多参数优化设计不仅计算工作量大且难以获得理论上最优解的问题,提出将全局敏感性分析和全局多梯度算法(PE-HMGE)相结合的多参数多目标优化策略。通过基于方差的Sobol全局敏感性分析精简系统模型,确定敏感参数,并构... 详细信息

针对复杂非线性系统多参数优化设计不仅计算工作量大且难以获得理论上最优解的问题,提出将全局敏感性分析和全局多梯度算法(PE-HMGE)相结合的多参数多目标优化策略。通过基于方差的Sobol全局敏感性分析精简系统模型,确定敏感参数,并构造基于敏感参数的多目标优化模型,采用PE-HMGE算法得到最优解。将本方法应用于主轴承润滑特性的简化模型优化设计中,对比仿真结果,最小油膜厚度(MOFT)提高了39%;最大峰值粗糙接触压力降低了45%;平均有效摩擦损失降低了19%。测试结果表明轴承的润滑特性得到明显改善,证明了该研究方法的有效性。

关键词：弹性液动轴承全局敏感性分析 PE-HMGE算法多参数优化

荧光和发光二极管辐射技术调控果蔬采后抗氧化活性及其机制研究进展

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食品科学 2019年第5期40卷 276-281页

作者：马亚丹张翠翠李林杰詹丽娟河南农业大学食品科学技术学院河南郑州450002

如何维持/增加果蔬采后抗氧化活性是当前果蔬采后贮藏保鲜研究的热点问题,光辐射技术作为当前一种新兴的物理保鲜方法,对果蔬采后抗氧化活性具有显著的调控作用。本文首先简要综述光辐射技术主要的光源类型及可能的保鲜机理,重点阐述光... 详细信息

如何维持/增加果蔬采后抗氧化活性是当前果蔬采后贮藏保鲜研究的热点问题,光辐射技术作为当前一种新兴的物理保鲜方法,对果蔬采后抗氧化活性具有显著的调控作用。本文首先简要综述光辐射技术主要的光源类型及可能的保鲜机理,重点阐述光辐射对果蔬采后抗氧化物质(VC、多酚类物质、类胡萝卜素)和抗氧化活性的调控作用,并对其可能的调控机制进行解析;最后就目前光辐射技术在果蔬采后贮藏保鲜研究和应用中存在的问题进行讨论。

关键词：光辐射抗氧化活性保鲜多酚

Increased mtDNA copy number promotes cancer progression by enhancing mitochondrial oxidative phosphorylation in microsatellite-stable colorectal cancer

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Signal Transduction and Targeted Therapy 2018年第1期3卷 246-254页

作者： Xiacheng Sun Lei zhan Yibing Chen Gang Wang linjie He Qian Wang Feng Zhou Fang Yang Jin Wu Yousheng Wu Jinliang Xing Xianli He Qichao Huang State Key Laboratory of Cancer Biology and Experimental Teaching Center of Basic Medicine Fourth Military Medical University710032 Xi’anChina Department of Gastroenterology Second Affiliated Hospital of Harbin Medical University150086 HarbinChina Center of Genetic&Prenatal Diagnosis First Affiliated HospitalZhengzhou University450052 ZhengzhouChina Department of General Surgery Tangdu HospitalFourth Military Medical University710032 Xi’anChina Department of General Surgery Huaihai HospitalXuzhou Medical UniversityXuzhou 221004China

Colorectal cancer is one of the leading causes of cancer death *** to global genomic status,colorectal cancer can be classified into two main types:microsatellite-stable and microsatellite-instable ***,the two subtypes also exhibit different responses to chemotherapeutic agents through distinctive molecular ***,mitochondrial DNA depletion has been shown to induce apoptotic resistance in microsatellite-instable colorectal ***,the effects of altered mitochondrial DNA copy number on the progression of microsatellite-stable colorectal cancer,which accounts for the majority of colorectal cancer,remain *** this study,we systematically investigated the functional role of altered mitochondrial DNA copy number in the survival and metastasis of microsatellite-stable colorectal cancer ***,the underlying molecular mechanisms were also *** results demonstrated that increased mitochondrial DNA copy number by forced mitochondrial transcription factor A expression significantly facilitated cell proliferation and inhibited apoptosis of microsatellitestable colorectal cancer cells both in vitro and in ***,we demonstrated that increased mitochondrial DNA copy number enhanced the metastasis of microsatellite-stable colorectal cancer ***,the survival advantage conferred by increased mitochondrial DNA copy number was caused in large part by elevated mitochondrial oxidative ***,treatment with oligomycin significantly suppressed the survival and metastasis of microsatellite-stable colorectal cancer cells with increased mitochondrial DNA copy *** study provides evidence supporting a possible tumor-promoting role for mitochondrial DNA and uncovers the underlying mechanism,which suggests a potential novel therapeutic target for microsatellite-stable colorectal cancer.

关键词： colorectal metastasis inhibited

Increased mtDNA copy number promotes cancer progression by enhancing mitochondrial oxidative phosphorylation in microsatellite stable colorectal cancer

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Increased mtDNA copy number promotes cancer progression by e...

可溶性糖介导的荧光照射延缓鲜切西兰花衰老黄化

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青...

作者： Xiacheng Sun Lei zhan Yibing Chen Gang Wang linjie He Qian Wang Fang Yang Jin Wu Yousheng Wu Jinliang Xing Xianli He Qichao Huang State Key Laboratory of Cancer Biology and Experimental Teaching Center of Basic Medicine Fourth Military Medical University Department of Gastroenterology Second Affiliated Hospital of Harbin Medical University

Aim:Colorectal cancer（CRC） is one of the leading causes of cancer death in *** to the global genomic status, CRC can be classified into two main types: microsatellite stable（MSS） and microsatellite instable（MSI） tumors. Moreover, the two subtypes also exhibit different responses to chemotherapeutic agents through distinctive molecular mechanisms. Recently,mtDNA depletion has been shown to induce apoptotic resistance in MSI CRC. However, the effects of alternated mtDNA copy number on the progression of MSS CRC, the majority of CRC, remain ***: In this study, we systematically investigated the functional role of altered mtDNA copy number in the survival and metastasis of MSS CRC cells. Moreover, the underlying molecular mechanisms were also explored. Results:Our results demonstrated that increased mtDNA copy number by forced TFAM expression significantly facilitated cell proliferation and inhibited apoptosis of MSS CRC cells both in vitro and in vivo, whereas mtDNA deletion either by TFAM knockdown or ethidium bromide treatment exhibited opposite effects. Moreover, we also demonstrated that increased mtDNA copy number enhanced the metastasis of MSS CRC cells. Mechanistically, the survival advantage conferred by increased mtDNA copy number was majorly caused by the elevated mitochondrial oxidative phosphorylation. Furthermore, treatment with oligomycin（inhibitor of ATP synthase）significantly suppressed the survival and metastasis of MSS CRC cells with increased mtDNA copy number. Conclusion:Our study provides the evidence supporting a possible tumor-promoting role for mtDNA and uncoveres the underlying mechanism, which suggests a potential novel therapeutic target for MSS CRC.

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食品科学 2019年第9期40卷 207-212页

作者：张翠翠马亚丹李林杰庞凌云詹丽娟河南农业大学食品科学技术学院河南郑州450002

鲜切西兰花极易衰老黄化,严重影响其感官质量和商品价值。为减缓鲜切西兰花衰老黄化,延长贮藏期,研究荧光照射(光照)处理(24μmol/(m^2·s)、12 h/d)对鲜切西兰花贮藏期间(7℃、5 d)衰老黄化的影响。结果表明:和黑暗对照相比,光照处理... 详细信息

鲜切西兰花极易衰老黄化,严重影响其感官质量和商品价值。为减缓鲜切西兰花衰老黄化,延长贮藏期,研究荧光照射(光照)处理(24μmol/(m^2·s)、12 h/d)对鲜切西兰花贮藏期间(7℃、5 d)衰老黄化的影响。结果表明:和黑暗对照相比,光照处理能够显著维持鲜切西兰花叶绿素含量,降低黄化指数(P<0.05),同时诱导葡萄糖、蔗糖和果糖累积,与贮藏初期(第0天)相比,3种糖在贮藏第5天分别增加了106%、73%和70%;进一步用0、40、80、120、160 mmol/L外源葡萄糖溶液对鲜切西兰花进行培养,结果发现在一定浓度范围(0～120 mmol/L)内,随着外源葡萄糖浓度增加,鲜切西兰花内源葡萄糖含量显著上升,黄化指数显著下降(P<0.05),而且黄化指数与外源葡萄糖浓度、内源葡萄糖含量呈极显著负相关(P<0.01),相关系数分别为-0.958和-0.878。因此,光照处理延缓鲜切西兰花黄化可能与其诱导的可溶性糖尤其是葡萄糖累积有关。

关键词：衰老荧光保鲜叶绿素可溶性糖

IGF1R和IGF2R在南江黄羊不同组织和肌细胞中的表达模式比较

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畜牧兽医学报 2019年第4期50卷 701-711页

作者：占思远丁雪仲涛王林杰李利张红平四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室成都611130

为了探讨IGF1R和IGF2R基因在山羊不同组织及肌细胞中mRNA的表达模式,本研究采用qRT-PCR检测了南江黄羊妊娠期3个阶段(E45、E60和E105)的背最长肌及出生后5个时期(B3、B30、B60、B90和B120)的肌肉组织(背最长肌、臂三头肌)和内脏(心、肝... 详细信息

为了探讨IGF1R和IGF2R基因在山羊不同组织及肌细胞中mRNA的表达模式,本研究采用qRT-PCR检测了南江黄羊妊娠期3个阶段(E45、E60和E105)的背最长肌及出生后5个时期(B3、B30、B60、B90和B120)的肌肉组织(背最长肌、臂三头肌)和内脏(心、肝、肺)中IGF1R和IGF2R的mRNA水平,以及骨骼肌卫星细胞(skeletal muscle satellite cells,SMSCs)增殖和分化过程中二者的表达模式。结果表明,在胎儿期的背最长肌中,IGF1R与IGF2R均呈现先上升后下降的表达趋势,其中IGF1R在E45和E60的表达量高于IGF2R(P0.05),在E105的表达量低于IGF2R(P<0.01)。在出生后阶段,IGF1R在肺中表达量最高,肝中最低;相反,IGF2R在肺中表达量最低,在背最长肌中最高。随着羔羊的发育,IGF1R与IGF2R的表达量在心中呈现上升的趋势,在肝中呈现"下降-上升-下降"的表达变化,而在肺中两者的表达趋势相反(B90前)。在背最长肌和臂三头肌中,IGF1R的表达量呈现持续上升模式(B90前),IGF2R则呈现出"下降-上升-下降"的趋势。此外,IGF1R和IGF2R在SMSCs增殖阶段(24、48和72 h)呈现"下降-上升"的表达模式,分化早期(0～3 d)低表达而后期显著增加(分化5 d,P<0.05),随后下降。本研究获得了IGF1R和IGF2R基因在南江黄羊不同组织及SMSCs中的表达模式,初步揭示IGF1R在山羊肺中的高表达与肝中的低表达对器官的生长和功能维持具有关键作用,IGF1R与IGF2R协同促进其肌肉组织发育和肌细胞的增殖分化。

关键词：南江黄羊 IGF1R IGF2R 组织卫星细胞基因表达