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Facile and Scalable Preparation of Fluorescent Carbon Dots for Multifunctional Applications

Engineering 2017年第3期3卷 402-408页

作者： Dan wang Zhiyong wang Qiuqiang zhan Yuan Pu jie-xin wang Neil R. Foster Liming Dai Beijing Advanced Innovation Center for Soft Matter Science and Engineering & State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical Technology Beijing 100029 China Center of Advanced Science and Engineering for Carbon (Case4Carbon) Department of Macromolecular Science and Engineering Case School of Engineering Case Western Reserve University Cleveland OH 44106 USA SCNU-ZJU Joint Research Center of Photonics South China Academy of Advanced Optoelectronics South China Normal University Guangzhou 510006 China Department of Chemical Engineering Curtin University Perth WA 6845 Australia

The synthesis of fluorescent nanomaterials has received considerable attention due to the great potential of these materials for a wide range of applications, from chemical sensing through bioimaging to optoelectron- ics. Herein, we report a facile and scalable approach to prepare fluorescent carbon dots （FCDs） via a one-pot reaction of citric acid with ethylenediamine at 150 ℃ under ambient air pressure. The resultant FCDs pos- sess an optical bandgap of 3.4 eV and exhibit strong excitation-wavelength-independent blue emission （λEm = 450 nm） under either one- or two-photon excitation. Owing to their low cytotoxicity and long fluorescence lifetime, these FCDs were successfully used as internalized fluorescent probes in human cancer cell lines （HeLa cells） for two-photon excited imaging of cells by fluorescence lifetime imaging microscopy with a high-contrast resolution. They were also homogenously mixed with commercial inks and used to draw fluo- rescent patterns on normal papers and on many other substrates （e.g., certain flexible plastic films, textiles, and clothes）. Thus, these nanomaterials are promising for use in solid-state fluorescent sensing, security labeling, and wearable optoelectronics.

关键词： Scalable Carbon dots Two-photon Fluorescence lifetime imaging Patterning

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Comparison of rAAV-2-EGFP versus liposome transfection of neural stem cells

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Neural Regeneration Research 2009年第11期4卷 822-826页

作者： Yanqiang zhan Wei Xiong jie Xu Bo Yin Furong wang Suming zhang Department of Neurology Tongji Hospital Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

BACKGROUND： At present, a universal method and vector for transfecting enhanced green fluorescent protein （EGFP） into neural stem cells does not exist. The traditional use of liposome to transfect GFP shows low labeling efficiency and short labeling time. However, there is an increasing number of reports in recent years utilizing adeno-associated virus （AAV） transfection of neural stem cells. OBJECTIVE： To compare differences of neural stem cell transfection via rAAV-2-EGFP or liposome, with regard to transfection efficiency, stability, and safety. DESIGN, TIME AND SETTING： A parallel, controlled experiment at a cellular molecular level was performed in the Central Laboratory, Clinical Neuromedicine Research Center, Tongji Medical College, Huazhong University of Science and Technology, between June 2007 and March 2008. MATERIALS： Liposome 2000 was purchased from Invitrogen, USA; rAAV-2-EGFP was offered from Beijing AGTC Gene Technology, China. METHODS： Cerebral cortical cells from embryonic day 12 C57BL/6 mouse embryo were isolated and cultivated, and the logarithmically growing neural stem cells were divided into three groups. Liposome transfection： neural stem cells were transfected with liposome/EGFP plasmid mixture comprising 2 pg pcDNA-3.0-EGFP plasmid and 12 μg Liposome 2000 in complete culture solution. AAV transfection： neural stem cells were transfected with virus transfection solution comprising rAAV-2-EGFP and complete culture solution at multiplicity of infection = 10^5. Negative control： physiological saline was used instead of virus transfection solution. MAIN OUTCOME MEASURES： At different time points after transfection （36 hours, 1 week, 2 weeks, 1 month, and 6 months）, the proportion of green fluorescent cells was quantified under fluorescent microscopy. Transfection efficiency and proliferative activity of the transfected neural stem cells were detected with flow cytometry and 3-（4,5）-dimethylthiahiazo （-z-yl）-3,5-di- phenytetrazoliumremide, respec

关键词： comparison neural stem cells transfection adeno-associated virus liposome

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Optimal Control for a Class of Complex Singular System Based on Adaptive Dynamic Programming

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IEEE/CAA Journal of Automatica Sinica 2019年第1期6卷 188-197页

作者： zhan Shi zhanshan wang the College of Information Science and Engineering Northeastern University the State Key Laboratory of Synthetical Automation for Process Industries(Northeastern University) IEEE

This paper presents a new design approach to achieve decentralized optimal control of high-dimension complex singular systems with dynamic uncertainties. Based on robust adaptive dynamic programming(robust ADP) method, controllers for solving the singular systems optimal control problem are designed. The proposed algorithm can work well when the system model is not exactly known but the input and output data can be measured. The policy iteration of each controller only uses their own states and input information for learning,and do not need to know the whole system dynamics. Simulation results on the New England 10-machine 39-bus test system show the effectiveness of the designed controller.

关键词： Adaptive dynamic programming (ADP) decentralized control frequency control power system singular systems

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基于特征融合和模板更新的孪生网络跟踪算法

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电光与控制 2025年第1期32卷 41-47,85页

作者：吴国瑞王峰李杰太原理工大学电子信息与光学工程学院山西晋中030000 太原理工大学电气与动力工程学院太原030000

针对现有孪生网络跟踪算法仅使用主干网络最后一层的特征进行相似度匹配,以及缺少有效模板更新策略的问题,提出基于多尺度特征融合和自适应模板更新的孪生网络跟踪算法。首先,结合深度过参数化卷积设计非填充单元,提取更深层的前景特征... 详细信息

针对现有孪生网络跟踪算法仅使用主干网络最后一层的特征进行相似度匹配,以及缺少有效模板更新策略的问题,提出基于多尺度特征融合和自适应模板更新的孪生网络跟踪算法。首先,结合深度过参数化卷积设计非填充单元,提取更深层的前景特征和语义背景;然后,设计新的全局-局部特征融合模块,充分聚合浅、中层特征的全局和局部信息,捕获丰富的浅层外观特征和中层过渡特征;最后,采用自适应模板更新机制在线更新模板。为验证算法的有效性,在公开数据集上对所提算法进行详尽评估,实验结果显示,所提算法在OTB2015和VOT2018数据集上的精确度分别达到0.878和0.588,GOT10K数据集上平均重叠率达到0.526,优于其他主流算法。

关键词：目标跟踪孪生网络计算机应用多层特征融合模板更新

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Transcriptional activation of Nlp by estrogen-ERa in breast cancer

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Science Bulletin 2017年第21期62卷 1445-1454页

作者： jia li jie chen liyan xue qimin zhan State Key Laboratory of Molecular Oncology Cancer Institute and HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBejing 100021China Laboratory of Molecular Oncology Peking University Cancer HospitalBeijing 100142.China Department of Pathology Cancer Institute and HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China

Estrogen Receptor-a （ERa） is the key transcription factor that regulates cell proliferation and homeosta- sis. In this pathway, estrogen plays an important role in genomic instability and cell cycle regulation pro- cesses and the mechanisms of its action are multifaceted. In this study, we showed that estrogen regulates genomic instability through promoting the expression of Nip, a BRCAl-associated centrosomal protein which is involved in microtubule nucleation, spindle formation, chromosomal missegregation and abnormal cytokinesis. We demonstrated that the expression of Nip is strongly associated with ERa and FOXAI level in clinical breast cancer samples with poor clinical outcomes to breast cancer patients. Addition of estrogen in the ER-positive breast cancer cells resulted in elevation of NLP mRNA. Significantly, we identified that estrogen-ERa is capable of regulating Nlp expression through specifically binding ERa to the proximal region and the Estrogen Responsive Elements （ERE） enhancer in the distal region of NLP gene. Reporter assays demonstrated that estrogen directly activated Nlp promoter. ChIP assay results showed that E2-ERa directly bound to the EREs of Nip. Therefore, overexpression of Nip in breast cancer exhibits a hormone-dependent pattern, and estrogen participates in the regulation of genome instability and cell cycle in breast cancer cells partially through transcriptional activation of NLP gene. Overexpression of Nlp enhances the malignant progression of ERa-positive breast cancer cells in vitro, whereas knockdown of Nip suppresses this biological effects in ERa-positive breast cancer ceils. ERa/NIp axis may serve as a promising target against breast cancer.

关键词： NipBreast cancerEstrogen receptor-or (ERct)1713-Estradiol (E2)Estrogen responsive elements (EREs)Transcriptional activation

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Synthesis and optical properties of highly efficient Na_(3)KMg_(7)(PO_(4))_(6):Eu^(2+)blue phosphor for full-spectrum white-light-emitting diodes:The role of Li_(2)CO_(3)flux

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Journal of Rare Earths 2024年第7期42卷 1217-1223,I0001页

作者： Zihao wang Sisi Liang Chenyang zhan Kunyuan Xu jie Hu Dejian Chen Liping Song Haomiao Zhu School of Physics and Materials Science Nanchang UniversityNanchang330000China Ganjiang Innovation Academy Chinese Academy of SciencesGanzhou341000China CAS Key Laboratory of Design and Assembly of Functional Nanostructures and Fujian Key Laboratory of NanomaterialsFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou350002China Xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials Xiamen Research Center of Rare Earth MaterialsHaixi InstituteChinese Academy of SciencesXiamen361021China

Full-spectrum phosphor-converted white-light-emitting diodes(pc-WLED)are emerging as a mainstream technology in semiconductor ***,high-performance blue phosphor which can be excited efficiently by a 400 nm NUV diode chip is still ***,we present a blue-emitting Na_(3)KMg_(7)(PO_(4))6:Eu^(2+)phosphor synthesized by the solid-reaction ***,we find that the using of Li_(2)CO_(3)as flux can significantly improve the crystal quality and thus the emission efficiency of the ***,the excitation peak of the phosphor shifts from 365 to 400 nm,which is pivotal for efficient NUV(400 nm)diode chip *** practical Eu^(2+)concentration is also enhanced by using Li_(2)CO_(3)as flux,and the absorption efficiency is greatly *** phosphor exhibits superior PL thermal stability,namely retains 94%integrated photoluminescence intensity at 150℃of that at 25℃.As a result,the optimized phosphor shows an emission band peaked at 437 nm with a bandwidth of 40 nm and a high external photoluminescence quantum yield of 51.7%.Finally,a pc-WLED was fabricated by using NKMPO:Eu^(2+)blue,Sr_(2)SiO_(4):Eu^(2+)green,CaAlSiN_(3):Eu^(2+)red phosphors,and a 400 nm NUV diode *** shows a high color rendering index of R_(a)=96.4 and a correlated color temperature of 4358 *** results prove that NKMPO:Eu^(2+)is a promising blue phosphor for full-spectrum WLED based on NUV diode chips.

关键词： Eu^(2+) Blue phosphor Na_(3)KMg_(7)(PO_(4))_(6) Full-spectrum white LEDs Rare earths

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Power Extraction Performance of a Semi-activated Flapping Foil in Gusty Flow

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Journal of Bionic Engineering 2017年第1期14卷 99-110页

作者： Jiapu zhan Bing Xu jie Wu Jing Wu Department of A erodynamics Nanjing University of Aeronautics and Astronautics Nanjing 210016 China Jiangsu Key Laboratory of Hi- Tech Research for Wind Turbine Design Nanjing 210016 China

A numerical investigation on the power extraction performance of a semi-activated flapping foil in gusty flow is conducted by using the commercial software FLUENT. The foil is forced to pitch around the axis at one-third chord and heave in the vertical direction due to the period lift force. Different from previous work with uniform flow, an unsteady flow with cosinusoidal velocity profile is considered in this work. At a Reynolds number of 1100, the influences of the mechanical parameters （spring constant and damping coefficient）, the amplitude and frequency of the pitching motion, the amplitude of the gust fluctuation and the phase difference between the pitching motion and the gusty flow on the power extraction performance are systematically investigated. Compared with the case of uniform flow, the capability energy harvesting of the system is enhanced by the introduction of the gusty flow. For a given pitching amplitude and frequency, the power extraction efficiency increases with the gust fluctuation amplitude. Moreover, with an optimal phase difference between pitch and gust （φ = 180°）, the efficiency can be further enhanced due to the generation of high lift force.

关键词： semi-activated flapping foil gusty flow power extraction performance

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Reducing the voltage loss of Y-series acceptor based organic solar cells via ternary/quaternary strategies

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Chinese Chemical Letters 2024年第2期35卷 70-86页

作者： Pengyu zhang Zhiyang zhang Heng Sun jie Li Yi Chen Jiang wang Chuanlang zhan Key Laboratory of Advanced Materials Chemistry and Devices (AMC&DLab) of the Department of Education of Inner Mongolia Autonomous Region Inner Mongolia Key Laboratory of Green CatalysisCollege of Chemistry and Environment ScienceInner Mongolia Normal UniversityHuhehot 010022China

In recent years, with the emergence of non-fullerene fused-ring acceptors, power conversion efficiencies (PCEs) of organic solar cells (OSCs) have exceeded 19%.However, compared to inorganic or perovskite photovoltaic cells, a higher voltage loss has become one of the key factors limiting further improvement in the PCEs of *** ternary/quaternary strategy has been identified as a feasible and effective way to obtain high-efficiency *** this review, a brief outline is given of the key roles that guest materials played in reducing voltage losses in solar cell devices and a brief look at the future material design and the design of ternary/quaternary systems.

关键词： Organic solar cell Ternary/quaternary strategy Open-circuit voltage Voltage loss Non-radiative recombination loss

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Advances in Phytochemistry and Modern Pharmacology of Saposhnikovia Divaricata(Turcz.)Schischk

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Chinese Journal of Integrative Medicine 2023年第11期29卷 1033-1044页

作者： GAO Jun-wen zhan Yang wang Yun-he ZHAO Shu-jie HAN Zhong-ming College of Resources and Environment Jilin Agricultural UniversityChangchun(130118)China Information Center Jilin Agricultural UniversityChangchun(130118)China College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun(130118)China

Saposhnikovia divaricata(Turcz.)Schischk(***,Fangfeng)is a herb in the Apiaceae family,and its root has been used since the Western Han Dynasty(202 B.C.).Chromones and coumarins are the pharmacologically active substances in *** phytochemical and pharmacological studies have demonstrated their antipyretic,analgesic,anti-inflammatory,antioxidant,anti-tumor,and anticoagulant *** and analytical strategy theory advancements have yielded novel results;however,most investigations have been limited to the main active substances—chromones and ***,we reviewed studies related to the chemical composition and pharmacological activity of ***,analyzed the developing trends and challenges,and proposed that research should focus on components’synergistic *** also suggested that,the structure-effect relationship should be prioritized in advanced research.

关键词： coumarin synergistic priori

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General Model of Temperature-dependent Modulus and Yield Strength of Thermoplastic Polymers

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Chinese Journal of Polymer Science 2020年第4期38卷 382-393,I0007页

作者： Ping-Yuan Huang zhan-Sheng Guo jie-Min Feng Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai UniversityShanghai200072China Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai 200072China

A general model was developed to predict the temperature-dependent modulus and yield strength of different thermoplastic *** model,which depends on only two parameters with clear and specific physical meanings,can describe the temperaturedependent modulus and yield strength of thermoplastic polymers over the full glass transition *** temperature-dependent modulus and yield strength of three thermoplastic polymers were measured by uniaxial tension tests over a temperature range of 243-383 *** predictions showed excellent agreement with the experimental *** analysis of model input parameters showed negligible effect on the present general *** universality of the present general model was further validated,showing excellent agreement with published experimental data on other thermoplastic polymers and their composites.

关键词： Thermoplastics Modulus Yield strength Modeling Glass transition

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