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Sex-and age-specific associations between abdominal fat and non-alcoholic fatty liver disease:a prospective cohort study

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Journal of Molecular Cell Biology 2023年第11期15卷 33-42页

作者： Hongli Chen Yuexing Liu Dan Liu Yebei Liang zhĳun zhu Keqing Dong Huating Li Yuqian Bao Jiarui Wu Xuhong hou Weiping Jia Department of Endocrinology and Metabolism Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai Diabetes InstituteShanghai Key Laboratory of Diabetes MellitusShanghai Key Clinical Center for Metabolic DiseaseShanghai 200233China General Practitioner Teams in Community Health Service Center of Nicheng Pudong New Area DistrictShanghai 201306China CAS Key Laboratory of Systems Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China

Obesity is closely related to non-alcoholic fatty liver disease(NAFLD).Although sex differences in body fat distribution have been well demonstrated,little is known about the sex-specific associations between adipose tissue and the development of *** community-based cohort data,we evaluated the associations between magnetic resonance imaging quantified areas of abdominal adipose tissue,including visceral adipose tissue(VAT)and subcutaneous adipose tissue(SAT),and incident NAFLD in 2830 participants(1205 males and 1625 females)aged 55–70 *** a 4.6-year median follow-up,the cumulative incidence rates of NAFLD increased with areas of VAT and SAT both in males and in *** analyses showed that the abovementioned positive associations were stronger in males than in females,especially in participants under 60 years *** contrast,these sex differences disappeared in those over 60 years ***,the risk of developing NAFLD increased non-linearly with increasing fat area in a sex-specific ***,sex-specific potential mediators,such as insulin resistance,lipid metabolism,inflammation,and adipokines,may exist in the associations between adipose tissue and *** study showed that the associations between abdominal fat and the risk of NAFLD were stratified by sex and age,highlighting the potential need for sex-and age-specific management of NAFLD.

关键词： visceral adipose tissue subcutaneous adipose tissue sex difference NAFLD incidence prospective study

Global Dynamic Characteristic of Nonlinear Torsional Vibration System under Harmonically Excitation

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Chinese Journal of Mechanical Engineering 2009年第1期22卷 132-139页

作者： SHI Peiming LIU Bin hou Dongxiao Institute of Electrical Engineering Yanshan University Qinhuangdao 066004 China

Torsional vibration generally causes serious instability and damage problems in many rotating machinery parts. The global dynamic characteristic of nonlinear torsional vibration system with nonlinear rigidity and nonlinear friction force is investigated. On the basis of the generalized dissipation Lagrange＇s equation, the dynamics equation of nonlinear torsional vibration system is deduced. The bifurcation and chaotic motion in the system subjected to an external harmonic excitation is studied by theoretical analysis and numerical simulation. The stability of unperturbed system is analyzed by using the stability theory of equilibrium positions of Hamiltonian systems. The criterion of existence of chaos phenomena under a periodic perturbation is given by means of Melnikov＇s method. It is shown that the existence of homoclinic and heteroclinic orbits in the unperturbed system implies chaos arising from breaking of homoclinic or heteroclinic orbits under perturbation. The validity of the result is checked numerically. Periodic doubling bifurcation route to chaos, quasi-periodic route to chaos, intermittency route to chaos are found to occur due to the amplitude varying in some range. The evolution of system dynamic responses is demonstrated in detail by Poincare maps and bifurcation diagrams when the system undergoes a sequence of periodic doubling or quasi-periodic bifurcations to chaos. The conclusion can provide reference for deeply researching the dynamic behavior of mechanical drive systems.

关键词： nonlinear torsional vibration dynamics behavior bifurcation chaos Melnikov's method

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系统调用异常检测模型研究

计算机工程与应用 2008年第35期44卷 92-94页

作者：张志利何聚厚陕西师范大学计算机科学学院西安710062

应用程序系统调用的执行序列可以体现出应用程序运行的行为特征,因此通过检测系统调用可以进行异常检测。针对已有算法模式库规模比较大的不足,提出了一种基于遗传算法的系统调用异常检测方法。首先用滑动窗口将系统调用序列划分成长度... 详细信息

应用程序系统调用的执行序列可以体现出应用程序运行的行为特征,因此通过检测系统调用可以进行异常检测。针对已有算法模式库规模比较大的不足,提出了一种基于遗传算法的系统调用异常检测方法。首先用滑动窗口将系统调用序列划分成长度固定的短序列,然后用遗传算法对系统调用短序列进行学习,建立模式库,用单模式不完全匹配方法对测试数据进行检测。实验表明该方法达到了较好的检测效果。

关键词：异常检测遗传算法系统调用短序列

TLR signaling in B-cell development and activation

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Cellular & Molecular Immunology 2013年第2期10卷 103-106页

作者： zhaoUn Hua Baidong hou Key Laboratory of Infection and Immunity Institute of Biophysics Chinese Academy of Sciences Beijing China

Expression of Toll-like receptors （TLRs） in B cells provides a cell-intrinsic mechanism for innate signals regulating adaptive immune responses. In combination with other signaling pathways in B cells, including through the B-cell receptor （BCR）, TLR signaling plays multiple roles in B-cell differentiation and activation. The outcome of TLR signaling in B cells is largely context-dependent, which partly explains discrepancies among in vitroand in vivostudies, or studies using different immunogens. We focus on recent findings on how B-cell-intrinsic TLR signaling regulates antibody responses, including germinal center formation and autoantibody production in autoimmune disease models. In addition, TLR signaling also acts on the precursors of B cells, which could influence the immune response of animals by shaping the composition of the immune system. With TLR signaling modulating immune responses at these different levels, much more needs to be understood before we can depict the complete functions of innate signaling in host defense.

关键词： autoimmunity B-cell development germinal center Toll-like receptor

See the color,see the seed:GmW1 as a visual reporter for transgene and genome editing in soybean

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The Crop Journal 2023年第1期11卷 311-315页

作者： Li Chen Shan Yuan Yupeng Cai Weiwei Yao Qiang Su Yingying Chen Jialing zhang Wensheng hou National Center for Transgenic Research in Plants Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

A fast and efficient recognition method of transgenic lines will greatly improve detection efficiency and reduce *** this study,we successfully identified the transgenic soybean plants by the *** isolated a GmW1 gene encoding a flavonoid 3'5'-hydroxylase from a soybean cultivar zh42(purple flower).We found that purple flowers occurred in the overexpression lines in the Jack and Williams 82 backgrounds(white flower).All plants with purple flowers were positive,and this trait seems stably inherited in the *** have also obtained the editing plants,which were classified into three types according to the different flower colors *** analyzed the phenotype and the homozygous types of the T_*** also found that a correspondence between flower color and stem *** study provides a visible color reporter on soybean *** can be quickly and early to identify the transgenic soybean plants by stem color of seedlings,which substantially reduces the amount of labor and cost.

关键词： Soybean GmW1 Color Transgenic lines Genome editing

Association between serum somatostatin levels and glucose-lipid metabolism in the Jino ethnic minority and Han Chinese population

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Science China(Life Sciences) 2018年第11期61卷 1382-1388页

作者： Shiyun Wang Rong zhang Xuhong hou Fusong Jiang Jie Wang zhen He Feng Jiang Cheng Hu Weiping Jia Shanghai Diabetes Institute Shanghai Key Laboratory of Diabetes MellitusShanghai Clinical Center for DiabetesShanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai 200233China Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus Shanghai 200233China

We aim to investigate the relationship between serum somatostatin(SST) levels and glucose-lipid metabolism at various stages of glucose tolerance in the Jino ethnic minority(n=111) and Han population(n=113) of Yunnan Province, southwest *** parameters and biochemical traits were measured. Serum SST and plasma glucagon levels were tested. Participants were divided into three subgroups: isolated fasting hyperglycemia(IFH), isolated post challenge hyperglycemia(IPH)and normal glucose tolerance(NGT). SST levels were found lower while glucagon levels were significantly higher in the Jino ethnic with IPH(P=0.0026 and P=0.0069, respectively). Fasting glucose and high density lipoprotein-cholesterol(HDL-C)levels were higher(P=0.0055 and P=0.0021, respectively) and fasting insulin levels and homeostasis model assessments β-cell function were lower(P=0.0479 and P=0.0007, respectively) in the Jino population. After adjusting for confounding factors, the serum SST level was associated with glucagon(P<0.0001) in both populations. The SST level was correlated with fasting Cpeptide(P=0.0267) in Jino and HDL-C levels in Han(P=0.0079). Our findings suggest that serum SST levels and plasma glucagon levels may vary in subjects with IPH between two ethnics.

关键词： somatostatin glucagon hyperglycemia Jino ethnic Han Chinese

冻存复苏条件对髓性白血病细胞株生物学特性的影响

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中国组织工程研究与临床康复 2007年第24期11卷 4714-4717页

作者：侯毅鞠袁忠海李艳郭素红吉林医药学院临床检验教研室吉林省吉林市132013

目的:采用不同方法对白血病HL60细胞株进行冻存和复苏,观察生物学特性改变,筛选最佳冻存复苏方案。方法:实验于2006-04/06在吉林医药学院临床检验实验室(国家三级标准)进行。①HL60细胞株由中国科学院上海生物细胞研究所提供。将欲冷冻... 详细信息

目的:采用不同方法对白血病HL60细胞株进行冻存和复苏,观察生物学特性改变,筛选最佳冻存复苏方案。方法:实验于2006-04/06在吉林医药学院临床检验实验室(国家三级标准)进行。①HL60细胞株由中国科学院上海生物细胞研究所提供。将欲冷冻保存的HL60细胞调整到良好的生长状态(对数生长期),随机数字表法分成4组,离心收集后在含体积分数为0.1小牛血清的RPMI-1640培养基中分别加入二甲基亚砜,使其终浓度依次为50,100,150,200g/L。冻存15d后,每组取1支冻存管复苏并接种于细胞培养板,培养12h后用锥虫蓝拒染法计算细胞复苏率。②选择冻存条件和冻存细胞密度相同的两支冻存管,按不同方法进行HL60细胞复苏。37℃水浴传统复苏法:立即将冻存管置于37℃水浴中,待融化后800r/min离心5min,吸去上清液,加入含体积分数为0.1小牛血清的RPMI-1640培养基,混匀后接种于细胞培养板,1mL/孔,9孔/组,置于体积分数为0.05的CO2培养箱37℃继续培养。40℃溶解后再37℃恒温改良复苏法:将冻存细胞于40℃水浴中迅速溶解,转入37℃水浴箱,融化后离心、加培养基等操作同传统复苏法。复苏细胞培养12h后采用锥虫蓝染色计算细胞存活率。③用无血清RPMI-1640培养基制备HL60细胞悬液,按3×107L-1密度接种于细胞培养板中,1mL/孔,然后分别加入体积分数为0.05,0.1,0.12,0.15,0.2的灭活小牛血清,每种血清浓度设置8个试验孔,并分别于培养12,24,36,48,60,72,84,96h后计数每孔细胞数量,并绘制细胞生长曲线。结果:①不同浓度二甲基亚砜冻存后HL60细胞复苏率的比较:二甲基亚砜200g/L组的细胞复苏率明显低于二甲基亚砜50,100,150g/L组[(64.6±2.8)%,(87.0±1.4)%,(86.4±2.1)%,(85.7±2.8)%;t=25.44,P0.05)。②不同复苏方法HL60细胞存活率的比较:与37℃水浴传统复苏法比较,40℃溶解后再37℃恒温改良复苏法的细胞存活率显著升高[(69.5±1.5)%,(87.4±1.8)%,t=23.24,P<0.01]。③RPMI-1640培养基不同血清含量对HL60细胞生长情况的影响:在体积分数为0.05的血清含量培养基中,HL60细胞基本不生长,且有逐渐死亡的趋势;在体积分数为0.1的血清含量培养基中,HL60细胞生长趋势明显,但生长速度相对较慢;在体积分数为0.12,0.15,0.2的血清含量培养基中,HL60细胞生长迅速,3种血清浓度间细胞生长趋势无明显差异。结论:以50g/L二甲基亚砜作为HL60细胞冻存保护剂、联合40℃溶解后再37℃恒温改良复苏法可使细胞保持最佳生物学特性,体积分数为0.12的血清含量为HL60细胞常规培养的最适浓度。

关键词：细胞培养复苏 HL60

多发性骨髓瘤患者微小残留病检测的现状、问题及展望

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中华医学杂志 2022年第36期102卷 2819-2822页

作者：侯健上海交通大学医学院附属仁济医院血液科上海200127

微小残留病(MRD)检测技术随着多发性骨髓瘤(MM)治疗的进展应运而生。它不仅能够反映疾病缓解的程度,还可以预测MM患者的预后,提示复发,且可以作为临床治疗决策的重要参考。长期持续MRD阴性已经成为全球公认的MM治疗目标,但MRD检测在临... 详细信息

微小残留病(MRD)检测技术随着多发性骨髓瘤(MM)治疗的进展应运而生。它不仅能够反映疾病缓解的程度,还可以预测MM患者的预后,提示复发,且可以作为临床治疗决策的重要参考。长期持续MRD阴性已经成为全球公认的MM治疗目标,但MRD检测在临床实践中仍有许多问题有待深入解决。本文就MRD检测在MM中应用取得的进展和存在的问题等方面进行阐述,并对未来发展方向进行展望,以进一步提升该病的诊治水平。

关键词：多发性骨髓瘤微小残留病诊断治疗

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The effect of sensation of transmission along meridian acupuncture for chronic nonbacterial prostatitis

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World Journal of Acupuncture-Moxibustion 2019年第2期29卷 113-118页

作者： Shu-ren MING Yu-lin FU Wen-guang hou Yue-lai CHEN Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine Shanghai 200437 China

Objective:To investigate the short-term efficacy difference between sensation of transmission along meridian acupuncture and non-sensation of transmission along meridian acupuncture in the treatment of chronic nonbacterial ***:A randomized,controlled,and single-blind clinical study was performed to collect 63 patients with chronic nonbacterial prostatitis who met the inclusion criteria and were randomly assigned to a transmission sensation group(31 patients)and a non-transmission sensation group(32 patients).The two groups of patients took the same acupuncture *** they were lying down on their backs,Guānyuán(关元 CV4),zhōngjí(中极 CV3)and Dàhè(大赫 K112)were *** they were lying down on their stomachs,Shènshū(肾俞 BL 23),Cìliáo(次髎 BL32)and Huìyáng(会阳 BL35)were *** them,the transmission sensation group was treated with transmission sensation along meridian to apply deep needling to the lower abdomen and lumbosacral acupuncture points with lifting,thrusting,and twirling method,so that the needling sensation was transmitted to the bladder,perineum and *** non-transmission sensation group was treated with non-transmission sensation along meridian,and the acupuncture points were treated with conventional acupuncture until the patient felt soreness and distending *** the arrival of *** groups of patients were treated with electro-acupuncture during acupuncture,and the sparse-dense wave(sparse wave 4 Hz,dense wave 20 Hz),and the needle retention continued for 20 *** patients were treated for 3 times with once every other day,and the treatment lasted for one *** CV4,CV3 and KI12 were used in the first and third treatments,and the BL23,BL32 and BL35 were used in the second *** National Institute of Health chronic prostatitis syndrome index(NIH-CPSI)and Hamilton Anxiety Rating Scale(HAMA)were observed,and the clinical efficacies of the patient were ***:After treatment,the NIH-CPSI scores(18.29±1.25,

关键词： Acupuncture Transmission sensation along meridian Chronic nonbacterial prostatitis