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Serum deprivation enhances DNA synthesis of human hepatoma SMMC-7721 cells

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World Journal of Gastroenterology 1998年第2期4卷 29-32页

作者： Jiang, SM Xu, zh Shandong Normal Univ Dept Biol Provincial Key Lab Anim Stress Jinan 250014 Shandong Provin Peoples R China

AIM To determine the relationship between serum deprivation or serum levels and cell proliferation of human hepatoma SMMC 7721 cells. METHODS Human hepatoma SMMC 7721 cells were grown in RPMI 1640 supplemented with 10% fetal calf (FCS) in 5% CO 2 incubator at 37℃ for 24h , and culture media were replaced to serum free or different serum FCS levels (2 5%, 5%, 10%, 20% and 25%). Six h, 12h , 18h and 24h after the culture, the cells were incorporated TdR for 4h . At last TdR incorporation was detected with liquid scintillation counting. RESULTS DNA synthesis of SMMC 7721 cells could be sharply stimulated by short time (6h) serum deprivation (the cpm value of 3H TdR incorporation of cells in serum free was 39 32 fold higher than cells in 25% serum), and the incorporation of 3H TdR was negatively related to the serum levels. Longer time serum starvation ( 12h , 18h and 24h ) also greatly stimulated DNA synthesis, although the cpm value of 3H TdR incroporation was less than that in 6h serum deprivation. Morphology of cells cultured in different serum levels also showed significant difference. CONCLUSIONS Compared with other cell lines such as BEL7404 and Swiss 3T3, human hepatoma SMMC 7721 cells had different response to the serum deprivation. Short time serum deprivation could greatly stimulate DNA synthesis of human hepatoma SMMC 7721 cells. Precautions must be given to the changes of serum levels for the detection of growth factors and drugs using SMMC 7721 cells as a model.

关键词： liver neoplasms carcinoma, hepatocellular DNA, neoplasm/biosynthesis SMMC 7721 tumor cell, cultured cell proliferation growth factors

EFFECT　OF　RARE　EARTH　ELEMENT　Er　ON　STREngTHENIng　OF　INTERFACE　OF　Ti（C，N）　BASED　CERMETS

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中国有色金属学会会刊：英文版 1996年第3期6卷 136-139页

作者： Liu, N Cui, K Hu, zh ANHUI INST TECHNOL DEPT MAT SCI & ENGNHEFEI 230069PEOPLES R CHINA HUAzh.ng UNIV SCI & TECHNOL WUHAN 430074PEOPLES R CHINA

ＥＦＦＥＣＴＯＦＲＡＲＥＥＡＲＴＨＥＬＥＭＥＮＴＥｒＯＮＳＴＲＥＮＧＴＨＥＮＩＮＧＯＦＩＮＴＥＲＦＡＣＥＯＦＴｉ（Ｃ，Ｎ）ＢＡＳＥＤＣＥＲＭＥＴＳ￥ＬｉｕＮｉｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｅｒｉａｌｓＳｃｉｅｎ... 详细信息

ＥＦＦＥＣＴＯＦＲＡＲＥＥＡＲＴＨＥＬＥＭＥＮＴＥｒＯＮＳＴＲＥＮＧＴＨＥＮＩＮＧＯＦＩＮＴＥＲＦＡＣＥＯＦＴｉ（Ｃ，Ｎ）ＢＡＳＥＤＣＥＲＭＥＴＳ￥ＬｉｕＮｉｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｅｒｉａｌｓＳｃｉｅｎｃｅａｎｄＥｎｇｉｎｅｅｒｉｎｇ，...

关键词： rare earth element Er Ti(C N) based cermets bending strength hardness

Nonlinear Analysis of Pile Foundation Jacket Platform

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China Ocean Engineering 1997年第1期12卷 21-28页

作者： Xu, B Shen, zh Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China

In this paper, the nonlinear collapse of the BOHAI-8 pile foundation jacket platform has been analyzed. The ultimate load and collapse process of two computational models of the structure are given. One model is of fixed support whose length is eight times the pile leg diameter and the other considers the nonlinearity of the soil-pile interaction.

关键词： nonlinear analysis jacket platform ultimate load platform collapse

DEVELOPMENT　OF　DISTILLATE　HDS　CATALYST

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催化学报 1996年第5期17卷 401-403页

作者： zh.ng Kongyuan zh ResearchinstituteofQiluPetrochemicalCompany Zibo25 ResearchinstituteofQiluPetroc

ＤＥＶＥＬＯＰＭＥＮＴＯＦＤＩＳＴＩＬＬＡＴＥＨＤＳＣＡＴＡＬＹＳＴＺｈａｎｇＫｏｎｇｙｕａｎ，ＺｈｏｕＲａｎ，ＺｈｅｎｇＳｈａｏｋｕａｎ，ＭａｏＰｅｎｇｓｈｅｎｇ（ＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅｏｆＱｉｌｕＰｅ... 详细信息

ＤＥＶＥＬＯＰＭＥＮＴＯＦＤＩＳＴＩＬＬＡＴＥＨＤＳＣＡＴＡＬＹＳＴＺｈａｎｇＫｏｎｇｙｕａｎ，ＺｈｏｕＲａｎ，ＺｈｅｎｇＳｈａｏｋｕａｎ，ＭａｏＰｅｎｇｓｈｅｎｇ（ＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅｏｆＱｉｌｕＰｅｔｒｏｃｈｅｍｉｃａｌＣｏｍｐａｎ...

关键词：发展馏出液加氢脱硫过程催化剂氧化铝氧化钼氧化钴热解汽油改性剂

Constructive proof on the existence of periodic solution of Duffing equation

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Chinese Science Bulletin 1997年第22期42卷 1870-1874页

作者： Li, WG Shen, zh Nanjing Univ Dept Math Nanjing 210093 Peoples R China

CONSIDER the following boundary value problem of Duffing equation:{x(t)+Cx’(t)+g(t,x)=e(t), x(0)-x’(zπ)=x’(0)-x’(2π)=0,} (1)where g: R×R→R is continuous and continuously differentiable with respect to x, continu-ous and 2π-periodic with respect to t. C is a constant. e: R→R is continuous and 2π-period-ic. If there exist two almost everywhere continuous functions a(t), b(t)

关键词： Duffing equation periodic solution continuation.

SYNTHESIS OF A 2-ARYLBENZOFURANOID DERIVATIVE

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Chinese Chemical Letters 1995年第4期6卷 289-292页

作者： Yan, zh Li, LN Beijing Union Med Coll Chinese Acad Med Sci Inst Mat Med Beijing 100050 Peoples R China

A derivative of 2-arylbenzofuranoid pattern fashioned after the naturally occurring isolates from Salviamiltiorrhiza was synthesized by Perkin condensation and oxidative ring closure.

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REACTION OF 3,7-DINITRODIBENZOBROMOLIUM BISULFATE WITHSOME NUCLEOPHILES

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Chinese Chemical Letters 1996年第6期7卷 505-506页

作者： Hou, ZJ zh.ng, H zh.ng, zh LANzhOU UNIV INST ORGAN CHEMLANZHOU 730000PEOPLES R CHINA LANzhOU UNIV STATE KEY LAB APPL ORGAN CHEMLANZHOU 730000PEOPLES R CHINA

Reaction of 3,7-dinitrodibenzobromolium bisulfate with some nucleophiles were studied. Primary products of reactions ore 2-bromo-2'-Nu-4-4'-dinitro-biphenyls.

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Inhibitory activity of polysaccharide extracts from three kinds of edible fungi on proliferation of human hepatoma SMMC-7721 cell and mouse implanted S180 tumor

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World Journal of Gastroenterology 1999年第5期5卷 404-407页

作者： Jiang SM Xiao ZM Xu zh Shandong Normal Univ Dept Biol Provincial Key Lab Anim Stress Jinan 250014 Shandong Prov Peoples R China Univ Heidelberg D-6900 Heidelberg Germany

AIM To determine the activities ofpolysaccharide extracts from Flammulina velutipes (Curt. ex Fr. ) Sing (FV), Lentinusedodes (LE) and Agaricus bisporus Sing (AB)on the proliferation of human hepatoma SMMC-7721 cells in vitro and on mouse implanted S-180tumors in *** The polysaccharide extracts were isolated from the fruit bodies of FV, LE and AB by the methods of hot-water extraction, Sevag’sremoval of proteins, ethanol precipitation,trypsin digestion and ethanol fractionalprecipitation. Human hepatoma SMMC-7721 cells were treated with 50 mg/L Polysaccharide extracts, and the mitosis index, mitochondria activity and cell proliferation were detected at different times in both control and experimental groups. The mice with S-180 implanted tumors were injected with the polysaccharide extracts at 24 mg/ kg body weight for 9 d and the tumorweight was measured on the 15th *** The mitosis index of hepatoma cells in vitro could be significantly decreased by treatment with the polysaccharide extracts fromthe three kinds of edible fungi (P ngi could inhibitnot only the Cultured malignant cells in vitfO butalso impIanted Sl80 tum0r i0 vivo.

关键词： polysaccharide, edible fungi liver neoplasm carcinoma, hepatocellular SMMC7721, tumor cell, cultured implanted tumor, S-180 cell proliferation

Recurrence or metastasis of HCC:predictors,early detection and experimental antiangiogenic therapy

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World Journal of Gastroenterology 2000年第1期6卷 61-65页

作者： Jiang YF Yang zh Hu JQ Cancer Institute Chinese Academy of Medical Sciences & Peking Union Medical CollegeBeijing 100021China

AIM To investigate the predictors forrecurrence or metastasis of HCC,and toevaluate the effect of antiangiogenic therapy onthe growth of transplantable human HCC in *** RT-PCR was used to measure theexpression of matrix metalloproteinase-9(MMP-9)and vascular endothelial growth factor(VEGF)in 56 pairs of nontumorous liver andtumor *** blood samples from humanHCC were examined by nested RT-PCR to find outAFP *** human endostatin andpolyclonal antibody against VEGF wereadministered to treat human HCC transplanted innude *** Thirty of 56 HCC samples showedstronger expression of MMP-9 in tumoroustissues than in nontumorous *** ofthe 26 patients with relative expression level ofMMP-9 more than 0.34 developed tumorrecurrence or metastasis,whereas only 7 of 30patients with relative expression level less than0.34 developed tumor recurrence(Png of HCCcells in circulation and a predictor of recurrenceor metastasis of *** may bean adjuvant therapy for HCC.

关键词： Subject headings carcinoma Hepatocellular neoplasm metastasis angiogenesis liver neoplasms