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Performance Comparison of Commercial and Home-Made Lipases for Synthesis of Poly(δ-Valerolactone)Homopolymers

Journal of Renewable Materials 2019年第4期7卷 335-343页

作者： Cansu Ulker zeynep gok Yuksel Guvenilir Istanbul Technical University Department of Chemical EngineeringAyazaga CampusIstanbul34469Turkey

Novozyme 435,which is the commercially available immobilized form of Candida antarctica lipase B,has been successfully conducted ring opening polymerization of lactones in organic *** this paper,it was aimed to introduce an alternative biocatalyst for Novozyme *** antarctica lipase B immobilized onto rice husk ashes via physical adsorption(with a specific activity of 4.4 U/mg)was prepared in previous studies and used as a biocatalyst for poly(δ-valerolactone)synthesis in the present *** reactions were proceeded at various reaction temperatures and periods via both two immobilized enzyme *** resulting products were characterized spectroscopically and *** highest molecular weight(Mn=9010 g/mol)was obtained via Novozyme 435 catalysis at 40℃and 24 *** performance of home-made lipase,which resulted in a molecular weight of 8040 g/mol,was close to commercial one.

关键词： Poly(δ-valerolactone) Candida antarctica lipase B Novozyme 435 immobilization enzymatic ring opening polymerization

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Toll-Like Receptor Gene Expression in Nasal Polyp

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International Journal of Otolaryngology and Head & Neck Surgery 2016年第2期5卷 90-95页

作者： Veysel Yurttas Tayfun Apuhan Tugce Simsek zeynep Ocak Uzeyir gok Department of Otorhinolaryngology and Head and Neck Surgery Abant Izzet Baysal University Faculty of Medicine Bolu Turkey Department of Otorhinolaryngology and Head and Neck Surgery Intercontinental Hospital Istanbul Turkey Department of Genetic Abant Izzet Baysal University Bolu Turkey Department of Otorhinolaryngology and Head and Neck Surgery Medical Park Hospital Elazig Turkey

Objective: To investigate the expression of Toll-like receptors (TLRs) and the correlation between TLR expression and mucosal changes in chronic sinusitis-related nasal polyposis. Methods: Patients with nasal polyps were enrolled in this study as the study group. The control group consisted of individuals undergoing surgery due to nasal obstruction caused by concha hypertrophy who were not diagnosed with chronic sinusitis-related nasal polyposis (NP). There were 30 patients in the study group (individuals with NP) and 20 patients in the control group. Sterile tissue samples were taken from the patients and were kept at -80°C. A piece of tissue sample was put into the eppendorf tube. Expression of TLR2 and TLR4 gene was investigated in the tissue samples. The samples were taken after the surgery;they were carried into the liquid nitrogen and were kept at -80° C before study. Results: TLR2 and TLR4 expression was measured in both groups and then analyzed using Student’s t-test. In the experimental group, the level of TLR2 expression was significantly higher than in the control group (P < 0.001);however, TLR4 expression was not significantly different between the two groups (P = 0.656). Conclusion: TLRs are thought to play a role in the etiopathogenesis of NP. In addition, the authors consider that a possible treatment for NP could be a TLR-specific treatment. Thus, in the future it will be essential to determine the extent to which TLRs function in NP formation and to apply this knowledge to design a specific therapeutic regimen for the enhanced treatment of NP.

关键词： Nasal Polyposis Toll-Like Receptor Gene Expression Etiopathogenesis

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