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建筑技艺（中英文） 2024年第4期30卷 76-80页

作者：赖冠廷孙佳爽梁喆上海大界机器人科技有限公司上海大界机器人科技有限公司智能建造事业部

针对建筑设计与制造间的断层,即设计与生产的“分离”,提出基于生产工艺的设计方法,包括集成式设计、面向生产与装配的设计以及材料与工艺的适配,并通过案例展示技术创新、工作流程优化对建筑设计的重要影响,以期实现“设计、生产、建... 详细信息

针对建筑设计与制造间的断层,即设计与生产的“分离”,提出基于生产工艺的设计方法,包括集成式设计、面向生产与装配的设计以及材料与工艺的适配,并通过案例展示技术创新、工作流程优化对建筑设计的重要影响,以期实现“设计、生产、建造”的紧密融合。

关键词：材料与工艺生产装配集成式设计数字化技术智能建造

Conventional processes and membrane technology for carbon dioxide removal from natural gas:A review

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Journal of Natural Gas Chemistry 2012年第3期21卷 282-298页

作者： zee Ying Yeo Thiam Leng Chew Abdul Rahman Mohamed Siang-Piao Chai Chemical Engineering Discipline School of EngineeringMonash University46150 Bandar SunwaySelangorMalaysia Faculty of Engineering and Green Technology(FEGT) Perak CampusUniversiti Tunku Abdul RahmanJalan UniversitiBandar Barat31900 KamparPerakMalaysia School of Chemical Engineering Engineering CampusUniversiti Sains MalaysiaSeri Ampangan14300 Nibong TebalPenangMalaysia

Membrane technology is becoming more important for CO,_ separation from natural gas in the new era due to its process simplicity, relative ease of operation and control, compact, and easy to scale up as compared with conventional processes. Conventional processes such as absorption and adsorption for CO2 separation from natural gas are generally more energy demanding and costly for both operation and maintenance. Polymeric membranes are the current commercial membranes used for CO2 separation from natural gas. However, polymeric membranes possess drawbacks such as low permeability and selectivity, plasticization at high temperatures, as well as insufficient thermal and chemical stability. The shortcomings of commercial polymeric membranes have motivated researchers to opt for other alternatives, especially inorganic membranes due to their higher thermal stability, good chemical resistance to solvents, high mechanical strength and long lifetime. Surface modifications can be utilized in inorganic membranes to further enhance the selectivity, permeability or catalytic activities of the membrane. This paper is to provide a comprehensive review on gas separation, comparing membrane technology with other conventional methods of recovering CO2 from natural gas, challenges of current commercial polymeric membranes and inorganic membranes for CO2 removal and membrane surface modification for improved selectivity.

关键词： membrane technology inorganic membrane CO2 separation natural gas: surface modification

Molecular cloning of rice cDNAs related to pollen development using the subtraction hybridization technique

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chinese Science Bulletin 1999年第20期44卷 1898-1903页

作者： zee S.Y Yau C.P. The Botany Department University of Hong Kong Hong Kong ChinaThe Botany Department University of Hong Kong Hong Kong China

The meiotic stage of pollen mother cell is a very important stage in controlling the development and formation of pollen. In order to clone the rice cDNA(s) of this stage, a normal rice, Annong N and its thermosensitive mutant, Annong S-1 were used as the plant material. The mRNA has been extracted from the young panicle at the meiotic stage. By using the cDNA subtraction hybridization technique, three cDNA fragments, RP-1, RP-2 and RP-3 have

关键词： cDNA subtraction hybridization pollen development rice.

Characteristics of unsteady flow in porous media while considering threshold pressure gradient with Green＇s function

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Journal of Central South University 2016年第1期23卷 201-208页

作者：曹仁义陈岭 Y.zee Ma 刘雪莹于柏慧 College of Petroleum Engineering China University of Petroleum Beijing 102249 China Schlumberger Company #400 Greenwood Village Colorado 80111 USA

The flow behavior in porous media with threshold pressure gradient(TPG) is more complex than Darcy flow and the equations of motion, and outer boundary and inner boundary with TPG are also different from Darcy flow for unsteady flow of a producing well in a reservoir. An analytic method to solve this kind of problem is in a need of reestablishment. The classical method of Green's function and Newman product principle in a new way are used to solve the unsteady state flow problems of various shapes of well and reservoir while considering the TPG. Four Green's functions of point, line, band and circle while considering the TPG are achieved. Then, two well models of vertical well and horizontal well are built and simultaneously the function to calculate the moving boundary of each well model is provided. The results show that when considering TPG the pressure field is much different, which has a sudden pressure change, with a moving boundary in it. And the moving boundary of each well model increases with time but slows down rapidly, especially when the TGP is large.

关键词： non-Darcy flow Green's function moving boundary threshold pressure gradient (TPG)

心肌补片:细胞来源、完善策略及最佳制作方法分析

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中国组织工程研究 2024年第17期28卷 2723-2730页

作者：胡威邢健陈广新陈泽娥赵艺乔丹欧阳昆富黄文华福建医科大学基础医学院福建省福州市350122 牡丹江医学院医学影像学院黑龙江省牡丹江市157011 北京大学深圳医院心外科广东省深圳市518036 北京大学深圳研究生院化学生物学与生物技术学院广东省深圳市518055 哈尔滨工业大学(深圳)教务部广东省深圳市518055 浙江大学医学院附属邵逸夫医院病理科浙江省杭州市310016 南方医科大学基础医学院人体解剖学国家重点学科广东省医学生物力学重点实验室广东省医学3D打印应用转化工程技术研究中心广东省广州市510515 深圳市坪山区人民医院(南方医科大学坪山总医院) 广东省深圳市518118 南方医科大学第三附属医院广东省医学3D打印应用转化创新平台广东省广州市510000

背景:心肌补片是一种修复损伤心肌的有效方式,但目前使用哪种细胞制作心肌补片和如何使心肌补片在体内发挥最大的治疗效果还存在争议。目的:通过综述心肌补片的细胞来源和完善心肌补片的策略来寻找出制作心肌补片的最佳方法。方法:由第... 详细信息

背景:心肌补片是一种修复损伤心肌的有效方式,但目前使用哪种细胞制作心肌补片和如何使心肌补片在体内发挥最大的治疗效果还存在争议。目的:通过综述心肌补片的细胞来源和完善心肌补片的策略来寻找出制作心肌补片的最佳方法。方法:由第一作者应用计算机以“cell sheet,cell patch,cardiomyocytes,cardia progenitor cells,fibroblasts,embryonic stem cell,mesenchymal stem cells”等为英文检索词检索PubMed和Web of Science数据库;以“心肌补片,生物3D打印,心肌”为中文检索词检索中国知网和万方数据库,经过入组筛选后,最终纳入94篇文献进入结果分析。结果与结论:①心肌补片的细胞来源主要分为3类:分别是体细胞、单能干细胞和多能干细胞。心肌补片的细胞来源丰富,但是并不是所有的细胞都适合制作心肌补片,例如,成纤维细胞和骨骼肌母细胞制成的心肌补片会有致心律失常的风险,间充质干细胞在体内的作用时间短并且存在伦理方面的问题。随着诱导式多功能干细胞的发现,为制作心肌补片提供了一个可靠的细胞来源。②心肌补片的制作方法有两种:一种是使用细胞片技术,另一种是使用生物3D打印技术。细胞片技术可完整保留细胞外基质成分,可以最大程度地模拟细胞在体内的生长循环,但是想要通过细胞片技术获得具有三维结构的心肌补片还存在困难。而生物3D打印技术可以通过计算机个性化设计获得具有三维结构的心肌补片。③完善心肌补片的策略主要包括:多种细胞共同培养后制作成心肌补片、改进生物3D打印技术中的墨水配方和支架成分、提高心肌补片的治疗效果、抑制移植后的免疫排斥反应和完善干细胞的分化培养方案。④目前还没有制作心肌补片的最佳细胞来源和制作方法,单靠某一种细胞或者某一种技术所获得的心肌补片常无法达到预期的治疗效果,因此研究者们在制作心肌补片之前需要根据预期的治疗效果来选择合适的策略制作心肌补片。

关键词：心肌补片多能干细胞 3D打印生物墨水细胞片综述间充质干细胞免疫反应心肌细胞骨骼肌母细胞

Membrane skeleton spectrin in pollen and pollen tube

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chinese Science Bulletin 1999年第10期44卷 930-933,961页

作者： Yan Li Longfei Yan S. Y. zee B. Q. Huang china Agr Univ Coll Biol Sci Beijing 100094 Peoples R China Univ Hong Kong Dept Bot Hong Kong Peoples R China

Immuno-western blots, immunofluorescence and immuno-gold labeling methods were used to study the existence and distribution of a homologue of the membrane skeleton spectrin in the pollen and pollen tube of Lilium davidii Duch. A spectrin homologue was found to exist in the pollen and pollen tube, distributed on membranes of secretory vesicles near Golgi apparatus.

关键词： membrane skeleton spectrin immuno-blots and label pollen plant cell.

Characterization of MAGEG2 with testis-specific expression in mice

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Asian Journal of Andrology 2017年第6期19卷 659-665页

作者： Juri Jeong Sora Jin Heejin Choi Jun Tae Kwon Jihye Kim Jaehwan Kim zee Yong Park Chunghee Cho School of Life Sciences Gwangju Institute of Science and Technology Gwangju 61005 South Korea.

Male germ cell development is a well-defined process occurring in numerous seminiferous tubules of the testis. Uncovering testicular novel genes related to intrinsic regulation of spermatogenesis is essential for the understanding of spermatogenesis. In the present study, we investigated mouse Mageg2, which belongs to a group of melanoma-associated antigens （MAGEs）. Mageg2 is transcribed in the testis specifically, and its expression level is increased at the pachytene spermatocyte stage, indicating that Mageg2 is expressed predominantly in germ cells. We generated an antibody against mouse MAGEG2 for further characterization at the protein level. Immunoblot analysis suggested that MAGEG2 has specific testicular expression and the expression primarily occurred in pachytene spermatocytes. Proteomic analyses demonstrated that mouse MAGEG2 binded to testicular germ cell-specific serine/threonine-protein kinase 31 （STK31） and heat shock protein 9 （HSPA9）. Direct binding with both interaction partners was confirmed by co-immunoprecipitation. We found that STK31 and HSPA9 bind MAGEG2 directly but not with each other. Interestingly, MAGEG2 reduced the kinase activity of STK31. Our study suggests that mouse MAGEG2 has at least two functions, including chaperone activity related to HSPA9 and regulation of pachytene spermatocyte-specific kinase, STK31. Altogether, our results provide the first information about MAGEG2 at the transcript and protein levels and suggest its potential molecular functions.

关键词： heat shock proteins serine/threonine kinase spermatogenesis testis

老年人肌肉减少症与心房颤动之间因果关联的孟德尔随机化分析

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老年医学与保健 2024年第2期30卷 290-296页

作者：申涛黄璨陈泽娥欧阳昆富葛建军韩振安徽医科大学附属省立医院心脏大血管外科安徽合肥230001 北京大学深圳医院心血管外科广东深圳518036

目的利用孟德尔随机化(Mendelian randomization,MR)方法探究肌肉减少症与心房颤动发病风险之间的因果关系,为老年人心房颤动的防治提供理论依据。方法基于全基因关联研究数据,以肌肉减少症的特征指标左手握力、右手握力和四肢肌肉质量(... 详细信息

目的利用孟德尔随机化(Mendelian randomization,MR)方法探究肌肉减少症与心房颤动发病风险之间的因果关系,为老年人心房颤动的防治提供理论依据。方法基于全基因关联研究数据,以肌肉减少症的特征指标左手握力、右手握力和四肢肌肉质量(appendicular lean mass,ALM)为暴露因素,AF为结局,通过MR评估左手握力、右手握力和ALM对AF的因果效应。结果逆方差加权法(inverse variance weighted,IVW)结果显示,左手握力(OR=0.782、95%CI:0.6770.904、P<0.001)、右手握力(OR=0.646、95%CI:0.5580.748、P<0.001)和ALM(OR=0.769、95%CI:0.7390.799、P<0.001)与心房颤动的发病风险降低相关,且结果无异质性和多效性。结论左手握力、右手握力和ALM增加可能有助于预防和治疗心房颤动。

关键词：肌肉减少症心房颤动孟德尔随机化衰老