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Optimizing ABA-based chemically induced proximity for enhanced intracellular transcriptional activation and modification response to ABA

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Science China Life Sciences 2024年

作者： zeng Zhou Yue-Qi Wang Xu-Nan Zheng Xiao-Hong Zhang Lu-Yao Ji Jun-You Han ze-cheng zuo Wei-Liang Mo Li Zhang College of Life Sciences Fujian Agriculture and Forestry University Basic Forestry and Proteomics Research Center Fujian Agriculture and Forestry University Jilin Province Engineering Laboratory of Plant Genetic Improvement College of Plant Science Jilin University

Abscisic acid(ABA)-based chemically induced proximity(CIP) is primarily mediated by the interaction of the ABA receptor pyrabactin resistance 1-like 1(PYL1) and the 2C-type protein phosphatase ABI1, which confers ABA-induced proximity to their fusion proteins, and offers precise temporal control of a wide array of biological processes. However, broad application of ABA-based CIP has been limited by ABA response intensity. In this study, we demonstrated that ABA-induced interaction between another ABA receptor pyrabactin resistance 1(PYR1) and ABI1 exhibited higher ABA response intensity than that between PYL1 and ABI1 in HEK293T cells. We engineered PYR1-ABI1and PYL1-ABI1 into ABA-induced transcriptional activation tools in mammalian cells by integration with CRISPR/dCas9 and found that the tool based on PYR1-ABI1 demonstrated better ABA response intensity than that based on PYL1-ABI1 for both exogenous and endogenous genes in mammalian cells. We further achieved ABA-induced RNA m6A modification installation and erasure by combining ABA-induced PYR1-ABI1 interaction with CRISPR/dCas13, successfully inhibiting tumor cell proliferation. We subsequently improved the interaction of PYR1-ABI1 through phage-assisted continuous evolution(PACE), successfully generating a PYR1 mutant(PYR1m) whose interaction with ABI1 exhibited a higher ABA response intensity than that of the wild-type. In addition, we tested the transcriptional activation tool based on PYRm-ABI1 and found that it also showed a higher ABA response intensity than that of the wild type. These results demonstrate that we have developed a novel ABA-based CIP and further improved upon it using PACE, providing a new approach for the modification of other CIP systems.

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A Study of the Blue-Light-Dependent Phosphorylation, Degradation, and Photobody Formation of Arabidopsis CRY2

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Molecular Plant 2012年第3期5卷 726-733页

作者： ze-cheng zuo Ying-Ying Meng Xu-Hong Yu zeng-Lin Zhang De-Shun Feng Shih-Fan Sun Bin Liu Chen-Tao Lin Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100080 China National Key Laboratory of Crop Biology Taian Subcenter of National Wheat Improvement Center College of Agronomy Shandong Agricultural University Taian Shandong 271018 China Department of Molecular Cell and Developmental Biology University of California Los Angeles CA 90095 USA

Arabidopsis cryptochrome 2 （CRY2） is a blue-light receptor mediating blue-light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation. CRY2 is a constitutive nuclear protein that undergoes blue-light-dependent phosphorylation, ubiquitination, photobody formation, and degradation in the nucleus, but the relationship between these blue-light-dependent events remains unclear. It has been proposed that CRY2 phosphorylation triggers a conformational change responsible for the subsequent ubiquitination and photobody formation, leading to CRY2 function and/or degradation. We tested this hypothesis by a structure-function study, using mutant CRY2-GFP fusion proteins expressed in transgenic Arabidopsis. We show that changes of lysine residues of the NLS （Nuclear Localization Signal） sequence of CRY2 to arginine residues partially impair the nuclear importation of the CRY2Ks41R and CRY2K554/sR mutant proteins, resulting in reduced phosphorylation, physiological activities, and degradation in response to blue light. In contrast to the wild-type CRY2 protein that forms photobodies exclusively in the nucleus, the CRY2K541R and CRY2K554/sR mutant proteins form protein bodies in both the nucleus and cytosol in response to blue light. These results suggest that photoexcited CRY2 molecules can aggregate to form photobody-like structure without the nucleus-dependent protein modifications or the association with the nuclear CRY2-interacting proteins. Taken together, the observation that CRY2 forms photobodies markedly faster than CRY2 phosphorylation in response to blue light, we hypothesize that the photoexcited cryptochromes form oligomers, preceding other biochemical changes of CRY2, to facilitate photobody formation, signal amplification, and propagation, as well as desensitization by degradation.

关键词： protein phosphorylation signal transduction fluorescence imaging protein degradation photobody

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Jiangsen Mao:a pioneer in Chinese hepatitis A vaccine and interferon research,a virologist at the forefront of times

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Protein & Cell 2024年第1期15卷 1-5页

作者： Huan Liu Kunlan zuo Zongzhen Wu Hao cheng University of Science and Technology of China Hefei 230026China State Key Laboratory of Virology Wuhan 430072China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China

There is a renowned virologist who dedicated his life to virology and medicine *** has made important contributions to the study of Chinese polio and hepatitis A vaccines,interferons,and the theory of viral reverse *** invented a live attenuated vaccine for hepatitis A(H2 strain)in the late 1970s,which contributed to the prevention of hepatitis A epidemics in China and earned him the title"Hepatitis A Eradicator"He said.

关键词： prevention hepatitis vaccine

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Switchable Multicomponent Cyclization Reactions to Access Fluoroalkylated Dihydropyrimidines and Pyrimidines under Solvent-Free Conditions

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Chinese Journal of Chemistry 2024年第12期42卷 1394-1398页

作者： Wanqing zuo Zhizhen Zhu Yu cheng Lingling zuo Xiao Geng Zhifang Li Lei Wang College of Material Chemistry and Chemical Engineering Key Laboratory of Organosilicon Chemistry and Material TechnologyMinistryof EducationHangzhouNormal UniversityHangzhouZhejiang311121China Advanced Research Institute and School of Pharmaceutical Sciences Taizhou UniversityJiaojiangZhejiang 318000China state Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032China

Comprehensive Summary The development of switchable solvent-free multicomponent reactions to build high-value-added products is an important demand for organic ***,we detailed the successful implementation of a switchable strategy for the construction of diverse 4-fluoroalkyl-1,4-dihydropyrimidines and 4-fluoroalkyl-pyrimidines via a solvent/additive-free[3+2+1]annulation,starting from readily available enamines,trifluoroacetaldehyde hydrate or 1-ethoxy-2,2-difluoroethanol and amidines *** reaction conforms to the concept of green synthesis,and provides a new avenue to access valuable fluorinated heterocycles.

关键词： Fluorine chemistry Multicomponent reactions Environmental chemistry Heterocycles Enaminones Amidines

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Developments and challenges on enhancement of photocatalytic CO_(2)reduction through photocatalysis

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Carbon Resources Conversion 2024年第3期7卷 106-120页

作者： Haiquan Wang Qingjie Guo Hongyan Zhang cheng zuo College of Chemistry&Chemical and Environmental Engineering Weifang UniversityWeifangPeople’s Republic of China State Key Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering College of Chemistry and Chemical EngineeringNingxia UniversityYinchuanPeople’s Republic of China

The conversion of CO_(2)into high-value fuels and chemicals has garnered research interest *** conversion and utilization of CO_(2)has become one of the most urgent tasks for *** this context,using solar energy to convert CO_(2)into high-value fuels such as CH4 and CH_(3)OH has extremely high potential application ***,the research progress and results of applying various photocatalysts in photocatalytic CO_(2)reduction with various novel catalysts were ***,strategies for improving photocatalytic performance were ***,improving the catalytic mechanism of catalysts and designing novel highactivity,high-stability catalysts through comprehensive exploration of the reaction mechanism were suggested to meet the future requirements of industrial production.

关键词： CO_(2)photoreduction Photocatalyst Charge separation CO_(2)conversion technology

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Distribution of modified Circumpolar Deep Water and its threat in Vincennes Bay,East Antarctica

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Journal of Oceanology and Limnology 2024年第5期42卷 1399-1414页

作者： Wenjun YE Lingqiao cheng Yujiro KITADE Song HU Juncheng zuo College of Oceanography and Ecological Science Shanghai Ocean UniversityShanghai 201306China Key Laboratory of Marine Ecological Monitoring and Restoration Technologies Ministry of Natural ResourcesShanghai 201306China Department of Ocean Sciences Tokyo University of Marine Science and TechnologyTokyo 108-8477Japan

The Antarctic Bottom Water formation site Vincennes Bay,East Antarctica is experiencing a substantial intrusion of modified Circumpolar Deep Water(mCDW),which may inhibit the formation of Dense Shelf Water(DSW)and drive basal melting of the ice *** on hydrographic data obtained from March to November in 2012,we evaluated the spatial spread of mCDW over the continental shelf region of Vincennes Bay and the associated temporal evolution of water properties,as well as the sea ice formation effect on water column in the coastal *** show that two branches of mCDW occupied the deep layers of the continental shelf,distinguished by the potential density(smaller than 27.8 kg/m^(3) or not)when potential temperatureθ=0.5°C in theθ-salinity *** warmer and less dense branch observed on the east plateau,accessed the eastern ice shelves in the coastal polynya to drive basal melting of ice *** contrast,the other colder and denser branch in the mid-depression reached the western Underwood Ice *** formation was detectable in the coastal polynya during September-November,proving the occurrence of deep *** heat loss and brine rejection during the intensive sea ice formation contributed to the destratification of the water column in the coastal *** was estimated that at least 1.11±0.79 TW heat carried by mCDW into the inner part of the polynya.

关键词： modified circumpolar deep water evolution of water properties coastal polynya ice shelf Vincennes Bay

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Design and fabrication of lightweight AlCrFeNiTi_(x) compositionally complex alloys with exceptional specific strength

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Rare Metals 2024年第7期43卷 3314-3328页

作者： Jun-Zhi Li Wei-Zong Bao Jie Chen Bo-Hua Yu Kun zuo Tian Gao ze-Yun Cai Guo-Qiang Xie School of Materials Science and Engineering Harbin Institute of Technology(Shenzhen)Shenzhen 518055China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001China

This study focuses on compositionally complex alloys(CCAs),aiming to achieve a balance between high strength and low density for new energy and aerospace *** composition of AlCrFeNiTi_(x) CCAs is strategically guided by employing density functional theory and the theoretical design of thermodynamic *** CCAs,particularly AlCrFeNiTi_(0.25) alloy,demonstrate remarkable specific yield strength(1640.8 MPa) and 22.7% maximum *** incorporation of Ti facilitates the formation of lightweight and high-strength L2_(1)phase,contributing to the overall high specific *** effects of grain boundary strengthening,solid solution strengthening,Orowan strengthening and Peierls flow stress further enhance *** exploration of micros tructural changes during fracture reveals the role of ordered phases in suppressing crack propagation and absorbing energy within disordered phases,thereby improving the toughness and fracture resistance of *** methods and discoveries establish a robust foundation for advancing the development of novel lightweight CCAs.

关键词： Lightweight Compositionally complex alloys Specific yield strength

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Surface-targeted functionalization of nickel-rich cathodes through synergistic slurry additive approach with multi-level impact using minimal quantity

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Nano Research 2024年第1期17卷 333-343页

作者： Jing Zhang Jiapei Li Longhao Cao Wenhua cheng Ziyin Guo Xiuxia zuo Chao Wang Ya-Jun cheng Yonggao Xia Yudai Huang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources College of ChemistryXinjiang UniversityUrumqi 830017China Ningbo Institute of Materials Technology&Engineering Chinese Academy of SciencesNingbo 315201China School of Materials Science and Engineering Tongji UniversityShanghai 200240China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

LiNi0.8Co0.1Mn0.1O_(2)(NCM811),a Ni-rich layered oxide,is a promising cathode material for high-energy density lithium-ion batteries(LIBs).However,its structural instability,caused by adverse phase transitions and continuous oxygen release,as well as deteriorated interfacial stability due to excessive electrolyte oxidative decomposition,limits its widespread *** address these issues,a new concept is proposed that surface targeted precise functionalization(STPF)of the NCM811 cathode using a synergistic slurry additive(SSA)*** approach involves coating the NCM811 particle surface with 3-aminopropyl dimethoxy methyl silane(3-ADMS),followed by the precise deposition of ascorbic acid via an acid-base *** slurry additives induce the formation of an ultra-thin spinel surface layer and a stable cathode–electrolyte interface(CEI),which enhances the electrochemical kinetics and inhibits crack *** STPF strategy implemented by the SSA approach significantly improves the cyclic stability and rate performance of the NCM811 cathode in both half-cell and full-cell *** work establishes a promising strategy to enhance the structural stability and electrochemical performance of nickel-rich cathodes and provides a feasible route to promote practical applications of high-energy density lithium-ion battery technology.

关键词： nickel-rich cathode slurry additive lithium-ion battery cathode–electrolyte interface ascorbic acid phase transition

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Investigation of wake-induced vibration interference between two staggered wave-cone cylinders at subcritical Reynolds number

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Journal of Hydrodynamics 2024年第2期36卷 378-393页

作者： Lin Zou Di-wei Liu Jian Liu Hong-cheng zuo Yin-huan Zheng Hao Xia School of Mechanical and Electronic Engineering Wuhan University of TechnologyWuhan430070China Department of Aeronautical and Automotive Engineering Loughborough UniversityLoughboroughUK

The wake induced vibration(WIV)of a one-and two-degree-of-freedom(1DOF,2DOF)downstream wave-cone cylinder(WCC)behind a stationary equal-size upstream wave-cone cylinder in the staggered arrangement is numerically investigated at subcritical Reynolds number of 3900 by using shear stressed transfer(SST)k-ωturbulence *** streamwise pitch ratios(P/Dm)vary from 4 to 6 with a fixed incident angleα=8°.Experimental measurements were also performed for the validation of the present numerical *** is found that the largest vibration amplitude in crossflow direction occurred at P/Dm=4,Ur=8 with small difference of streamwise vibration at P/Dm=4,*** from single wavy-cone cylinder(SWCC),the downstream flexible one of a pair staggered WCCs got larger vibration amplitude during phase switching stage instead of in-phase *** upstream wake will suppress the triple frequency of main frequency in the power spectra density(PSD)functions of Cl but stimulate the double one of *** intriguing vibration mechanism happened in all 2DOF cases where the trajectory of downstream WCC is a significant ellipse rather than a figure of *** transformation of phase switching and the variation of the main frequency of drag coefficient(Cd)can be explained by the vortex-shedding modes of downstream WCC.

关键词： Two wave-cone cylinders staggered arrangement wake induced vibration phase switching vortex shedding

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Research progress of MOF-based materials in the photocatalytic CO_(2)reduction

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Carbon Resources Conversion 2024年第1期7卷 103-121页

作者： Qian Su Qingjie Guo Haiquan Wang Meifang Liu cheng zuo College of Chemistry&Chemical and Environmental Engineering Weifang UniversityWeifangPeople’s Republic of China State Key Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering College of Chemistry and Chemical EngineeringNingxia UniversityYinchuanPeople’s Republic of China

Photocatalytic technology could utilize solar energy to reduce CO_(2)into high-value-added fossil fuels,providing promising solutions for global energy and environmental ***-organic frameworks(MOFs)are a class of crystalline porous solids with high porosity and flexible ***-based photocatalysts have excellent CO_(2)capture ability,photochemical and structural characteristics and have shown infinite development potential in CO_(2)***,in practical large-scale applications,MOF-based photocatalysts still have some urgent problems to be solved,such as high composite rate of photogenerated carriers,limited response range to visible spectrum,poor photocatalytic activity and weak reduction *** paper introduces series of MOF-based photocatalysts,including pure MOF materials,compounds,and derivatives,were reviewed based on recent *** was placed on the modification strategy of photocatalysts,the photocatalytic reaction’s key physical and chemical parameters,and the mechanism of synergistic improvement of chemical fuel *** and most importantly,the future development trends and prospects of MOF-based catalysts for photocatalytic CO_(2)reduction were discussed.

关键词： Photocatalysis Metal-organic frameworks Rational design CO_(2)reduction

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