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International Journal of Minerals,Metallurgy and Materials 2017年第12期24卷 1412-1423页

作者： Hong-xiang Li Xin-yu Nie zan-bing he Kang-ning Zhao Qiang Du Ji-shan Zhang Lin-zhong Zhuang State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China SINTEF Materials and Chemistry Pb.124 BlindernNO-0314 OsloNorway IJmuiden Technology Centre Tata Steel Research&Development1970 CA IJmuidenThe Netherlands

Ti-6Al-4V/Al7050 joints were fabricated by a method of insert molding and corresponding interfacial microstructure and mechanical properties were investigated. The interfacial thickness was sensitive to holding temperature during the first stage, and a good metallurgical bonding interface with a thickness of about 90 μm can be obtained at 750°C. X-ray diffraction, transmission electron microscopy, and thermodynamic analyses showed that the interface mainly contained intermetallic compound TiAl_3 and Al matrix. The joints featured good mechanical properties, i.e., shear strength of 154 MPa, tensile strength of 215 MPa, and compressive strength of 283 MPa, which are superior to those of joints fabricated by other methods. Coherent boundaries between Al/TiAl_3 and TiAl_3/Ti were confirmed to contribute to outstanding interfacial mechanical properties and also explained constant fracture occurrence in the Al matrix. Follow-up studies should focus on improving mechanical properties of the Al matrix by deformation and heat treatment.

关键词： interfacial microstructure interfacial bonding mechanism mechanical properties insert molding method coherent boundaries Ti/Al joints

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一种实用的风力机叶片气动设计方法研究

空气动力学学报 2012年第3期30卷 411-414,379页

作者：昝丙合杨旭东西北工业大学翼型叶栅国家重点实验室陕西西安710072

提出了水平轴风力机叶片的优化设计模型,该模型以风轮平均年能量输出最大为设计目标,并将风速的概率分布考虑进来。为了获得较好的优化结果,首先采用PROPID程序对叶片进行反设计,获得较好性能的叶片几何,在此基础上,运用遗传算法进行搜... 详细信息

提出了水平轴风力机叶片的优化设计模型,该模型以风轮平均年能量输出最大为设计目标,并将风速的概率分布考虑进来。为了获得较好的优化结果,首先采用PROPID程序对叶片进行反设计,获得较好性能的叶片几何,在此基础上,运用遗传算法进行搜索寻优,这样不仅减小了搜索的范围,也降低了搜索的时间。对叶片面积附加约束,以获得符合实际的叶片,采用5阶贝塞尔函数在设计点之间进行插值,以获得光滑的几何外形。利用该方法优化设计了600kW风力机叶片。与已有叶片相比,优化结果显示了明显的优越性,验证了该优化方法的有效性和实用性。

关键词：风力机优化设计反设计 PROPID 遗传算法

YTE-17 inhibits colonic carcinogenesis by resetting antitumor immune response via Wnt5a/JNK mediated metabolic signaling

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Journal of Pharmaceutical Analysis 2024年第4期14卷 525-541页

作者： Hua Sui Wanli Deng Qiong Chai bing Han Yuli Zhang Zhenzhen Wei zan Li Ting Wang Jiling Feng Man Yuan Qingfeng Tang Hongxi Xu Medical Experiment Center Jiading Branch of Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai201803China Translational Medicine Research Center for Cancer Prevention and Treatment Shanghai General Hospital Jiading Branch-School of Pharmacy of Shanghai University of Traditional Chinese Medicine Joint LaboratoryShanghai201803China Department of Medical Oncology Putuo HospitalShanghai University of Traditional Chinese MedicineShanghai200062China School of Pharmacy Shanghai University of Traditional Chinese MedicineShanghai201203China The Second Clinical Medical College of henan University of Traditional Chinese Medicine Zhengzhou450000China Shanghai University of Traditional Chinese Medicine Shanghai201203China Precision Research Center for Refractory Diseases Institute for Clinical ResearchShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai201620China

The density and composition of lymphocytes infiltrating colon tumors serve as predictive factors for the clinical outcome of colon *** previous studies highlighted the potent anti-cancer properties of the principal compounds found in Garcinia yunnanensis(YTE-17),attributing these effects to the regu-lation of multiple signaling ***,knowledge regarding the mechanism and effect of YTE-17 in the prevention of colorectal cancer is *** this study,we conducted isobaric tags for relative and absolute quantification(iTRAQ)analysis on intestinal epithelial cells(IECs)exposed YTE-17,both in vitro and in vivo,revealing a significant inhibition of the Wnt family member 5a(Wnt5a)/c-Jun N-terminal kinase(JNK)signaling ***,we elucidated the influence and mechanism of YTE-17 on the tumor microenvironment(TME),specifically focusing on macrophage-mediated T helper 17(Th17)cell induction in a colitis-associated cancer(CAC)model with Wnt5a ***,we performed the single-cell RNA sequencing(scRNA-seq)on the colonic tissue from the Wnt5a-deleted CAC model to characterize the composition,lineage,and functional status of immune mesenchymal cells during different stages of colorectal cancer(CRC)***,our findings demon-strate a significant reduction in M2 macrophage polarization and Th17 cell phenotype upon treatment with YTE-17,leading to the restoration of regulatory T(Treg)/Th17 cell balance in azoxymethane(AOM)/dextran sodium sulfate(DSS)***,we also confirmed that YTE-17 effectively inhibited the glycolysis of Th17 cells in both direct and indirect co-culture systems with M2 ***,our study shed light on potential mechanisms linking the non-canonical Wnt5a/JNK signaling pathway and well-established canonical b-catenin oncogenic pathway in ***,we proposed that Wnt5a/JNK signaling activity in IECs promotes the development of cancer stem cells with b-catenin activity within

关键词： Tumor microenvironment Intestinal epithelial cells Treg/Th17 cells Metabolism Wnt5a/JNK signaling Tumorigenesis

Suppressive Effects of Plumbagin on Invasion and Migration of Breast Cancer Cells via the Inhibition of STAT3 Signaling and Down-regulation of Inflammatory Cytokine Expressions

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Bone Research 2013年第4期1卷 362-370页

作者： Wei Yan bing Tu Yun-yun Liu Ting-yu Wang Han Qiao zan-jing Zhai Hao-wei Li Ting-ting Tang Shanghai Key Laboratory of Orthopaedic Implants Department of Orthopaedic SurgeryShanghai Ninth People's HospitalShanghai Jiao Tong University School of Medicine Wendeng Zhenggu Hospital of Shandong Province Department of Gynecology and Obstetrics Wendeng Center Hospital of Weihai City Department of Pharmacy Shanghai Ninth People's HospitalShanghai Jiao Tong University School of Medicine

Objective： The aim of this study was to investigate the effects of plumbagin （PL）, a naphthoquinone derived from the medicinal plant plumbago zeylanica, on the invasion and migration of human breast cancer cells. Methods： Human breast cancer MDA-MB-231SArfp cells were treated with different concentrations of plum- bagin for 24 h. The effects of plumbagin on the migration and invasion were observed by a transwell method. The expressions of IL-1α, IL-1β, IL-6, IL-8, TGF-β, TNFα, MMP-2 and MMP-9 mRNA in MDA-MB-231SArfp cells were detected using Real-Time PCR. MDA-MB-231SArfp cells were treated with plumbagin at different concentrations for 45 minutes. The activation of STAT3 was detected by western blot. Following this analysis, STAT3 in MDA-MB-231SArfp cells was knocked out using specific siRNA, mRNA levels of IL-1α, TGF-β, MMP-2 and MMP-9 were then detected. Consequently, MDA-MB-231SArfp cells were injected intracardially into BALB/c nude mice to construct a breast cancer bone metastatic model. The mice were injected intra- peritoneally with plumbagin. Non-invasive in vivo monitoring, X-ray imaging and histological staining were performed to investigate the effects of plumbagin on the invasion and migration of breast cancer cells in vivo. Results： The in vitro results showed that plumbagin could suppress the migration and invasion of breast cancer cells and down-regulate mRNA expressions of IL-1α TGF-β, MMP-2 and MMP-9. Western blotting demonstrated that plumbagin inhibited the activation of STAT3 signaling in MDA-MB-231SArfp cells. The inactivation of STAT3 was found to have an inhibitory effect on the expressions of IL-1α, TGF-β, MMP-2 and MMP-9. In vivo studies showed that plumbagin inhibited the metastasis of breast cancer cells and decreased osteolytic bone metastases, as well as the secretion of MMP-2 and MMP-9 by tumor cells at metastatic lesions. Conclusions： Plumbagin can suppress the invasion and migration of breast cancer cells via the inhibition of STAT3

关键词： plumbagin breast cancer invasion migration

Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichoderma viride endochitinase gene

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Journal of Integrative Agriculture 2015年第3期14卷 430-437页

作者： YU Ya ZHANG Lei LIAN Wei-ran XU Feng-feng LI Shuang-tao XIANG Juan ZHANG Guo-zhen HU zan-min ZHAO bing REN Shu-xin GUO Yang-dong College of Agriculture & Biotechnology China Agricultural University Department of Biology Science & Technology Wuhan Institute of Bioengineering Institute of Genetics & Developmental Biology Chinese Academy of Sciences School of Agriculture Virginia State University

A endochitinase gene（Tch） from the fungus Trichoderma viride was introduced into broccoli（Brassica oleracea var. italica） by Agrobacterium-mediated transformation. Sixty-eight putative transformants were obtained and the presence of the Tch gene was confirmed by both PCR and Southern blot analysis. RT-PCR analysis showed an accumulation of the transcript encoding the endochitinase protein in the transgenic plants. Using real-time quantitative PCR, the expression profiling of endochitinase gene was analyzed. Primary transformants and selfed progeny were examined for expression of the endochitinase using a fluorometric assay and for their resistance to the pathogenic fungi Botrytis cinerea and Rhizoctonia solani. The endochitinase activities in T0 in vitro plants, T0 mature plants and T1 mature plants were correlated with leaf lesions, and the transgenic line T618 had high endochitinse activities of 102.68, 114.53 and 120.27 nmol L–1 MU min–1 mg–1 protein in the three kinds of plants, respectively. The endochitinase activity showed a positive correlation with the resistance to the pathogens. Most transgenic T0 broccoli had increased resistance to the pathogens of B. cinerea and R. solani in leaf assays and this resistance was confirmed to be inheritable. These findings suggested that expression of the Tch gene from T. viride could enhance resistance to pathogenic fungi in Brassica species.

关键词： Botrytis cinerea broccoli endochitinase gene genetic transformation Rhizoctonia solani

RNA interference-mediated gene silencing of vascular endothelial growth factor in colon cancer cells

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World Journal of Gastroenterology 2007年第40期13卷 5312-5316页

作者： Tie-Jun Li Jian-Ning Song Kai Kang Shu-Sheng Tong zan-Lan Hu Tong-Chuan he bing-Qiang Zhang Cai-Quan Zhang The First Affiliated Hospital Chongqing University of MedicalSciences Chongqing 400016 China The Ninth Hospital of Chongqing Chongqing 400700 China Molecular Oncology Laboratory University of Chicago Medical Center IL 60637 United States

AIM： TO inhibit the expression of vascular endothelial growth factor （VEGF） in colon cancer cell line by RNA interference （RNAi）.METHODS： Followed the service of E-RNAi, we designed and constructed two kinds of shRNA expression vectors aiming at the VEGF gene, then transfected them into colon cancer HT29 cells by lipofectamineTM 2000. The level of VEGF mRNA was investigated by RT-PCR and Northern blotting. The protein expression of VEGF was observed by immunofluoresence staining and Western blotting.RESULTS： We got two kinds of VEGF specific shRNA expression vectors which could efficiently inhibit the expression of VEGF in HT29 cells. RT-PCR, Northern blotting, immunofluoresence staining and Western blotting showed that inhibition rate for VEGF expression was up to 42%, 89%, 73% and 82%, respectively.CONCLUSION： The expression of VEGF can be inhibited by RNA interference in HT29 cells.

关键词： RNA interference Vascular endothelialgrowth factor Colon cancer Northern blotting Western blotting

内镜超声指导内镜下皮圈套扎联合高频电切除术对胃异位胰腺的诊断和治疗价值

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中国内镜杂志 2008年第11期14卷 1168-1170页

作者：余福兵何夕昆张冰左赞云南省第二人民医院消化科云南昆明650021 云南省第二人民医院肝胆外科云南昆明650021

目的提高对胃异位胰腺的诊断及治疗水平。方法分析20例来源于黏膜下层的胃黏膜下肿瘤(SMT)患者的超声内镜(EUS)特征,对其行内镜下皮圈套扎联合高频电切除术,并将检查结果与术后病理结果进行对照。结果20例SMT患者EUS诊断胃异位胰腺17例... 详细信息

目的提高对胃异位胰腺的诊断及治疗水平。方法分析20例来源于黏膜下层的胃黏膜下肿瘤(SMT)患者的超声内镜(EUS)特征,对其行内镜下皮圈套扎联合高频电切除术,并将检查结果与术后病理结果进行对照。结果20例SMT患者EUS诊断胃异位胰腺17例,脂肪瘤3例。病理组织学检查后确诊异位胰腺18例,脂肪瘤2例。EUS诊断准确率达94.4%。结论超声内镜对胃异位胰腺的诊断有一定的价值,内镜切除是安全有效的治疗方法。

关键词：胃异位胰腺超声内镜诊断治疗

New Structure for Multi-Channel Demultiplexing

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The Journal of China Universities of Posts and Telecommunications 1997年第1期4卷 21-28页

作者： Li bing, Shi zan and Ye Peida (Optical Communication Laboratory, Beijing University of Posts and Telecommunications, Beijing 100088, P. R.China)

A new all optical demultiplexer based on nonlinear effect in semiconductor laser amplifier (SLA) is proposed. It can demultiplex all channels of the OTDM signal concurrently, and it can also be integrated on a single chip. The proposed device consists of a series short Fabry Perot amplifiers (FPSLA) setting on the path vertically, through which the preamplified optical signal travels. Perpendicularly to the signal, K(channel number) paralleled beams of light pulse, which act as probes with repetition at the bit rate of one channel, irradiate on these FP SLAs , respectively, just when the corresponding signal channel pulse passes through the active region from lateral side. The transmissivity depends on the signal pulse. Theoretical analysis has been given. As a demonstrating example, a device operated at the speed of 25Gbit/s (2.5Gbit/s×10 ) is simulated and the results show that the about 9.24 dB extinction ratio can be achieved easily.

关键词： optical time division multiplexing demultiplexer Fabry Perot semiconductor laser amplifier

全球(Z)双三次数值模式的设计与个例模拟(I):模式动力框架设计

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高原气象 2008年第3期27卷 474-480页

作者：辜旭赞张兵中国气象局武汉暴雨研究所湖北武汉430074

引入双三次数值模式:双三次数值模式是通过作三次样条与双三次曲面拟合,实现各个大气要素量场的二阶可导,从而可对各个预报方程作时间积分。双三次数值模式适合采用大气运动原始方程组和采用水平方向准拉格朗日/垂直方向欧拉时间积分方... 详细信息

引入双三次数值模式:双三次数值模式是通过作三次样条与双三次曲面拟合,实现各个大气要素量场的二阶可导,从而可对各个预报方程作时间积分。双三次数值模式适合采用大气运动原始方程组和采用水平方向准拉格朗日/垂直方向欧拉时间积分方案。且本文的全球(Z)双三次数值模式与个例模拟实际采用Navier-Stokes"浅薄大气"原始方程组,建立球面Z坐标系上的非静力、全可压、干/湿绝热大气运动动力框架。其离散化气压、气温预报方程与个例模拟揭示出大气运动中凝结降水,其天气学原因不仅是湿空气作Z坐标垂直上升运动,而且是湿空气被"减压/减温",即湿空气作P坐标"垂直上升运动",后者可由大气平流运动(如Rossby波)引起。

关键词：双三次数值模式原始方程组非静力全可压大气运动动力框架准拉格朗日/欧拉时间积分方案大气运动降水物理机制

全球(Z)双三次数值模式的设计与个例模拟(Ⅱ):个例模拟结果分析

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高原气象 2008年第3期27卷 481-490页

作者：辜旭赞张兵中国气象局武汉暴雨研究所湖北武汉430074

引入双三次数值模式:通过"双三次曲面拟合—时间步长积分—双三次曲面拟合—……",实现成为一种新算法的数值模式。在球面Z坐标系上,给出全球经纬网格双三次数值模式的一个设计。全球(Z)双三次数值模式采用非静力、全可压、干/湿绝热大... 详细信息

引入双三次数值模式:通过"双三次曲面拟合—时间步长积分—双三次曲面拟合—……",实现成为一种新算法的数值模式。在球面Z坐标系上,给出全球经纬网格双三次数值模式的一个设计。全球(Z)双三次数值模式采用非静力、全可压、干/湿绝热大气运动动力框架,并采用显式水平方向准拉格朗日/垂直方向欧拉时间积分方案,用NCEP再分析资料做初值场,初步模拟出从极地到热带的基本大气环流。模拟试验表明:因大气运动本质上是非线性的,当限定模式大气作线性"一次运动"时,个例模拟无须任何平滑;但限定模式大气作"三次运动"时,必须有适当平滑。本文认为,未来双三次数值模式可按场变量双三次曲面曲率,以判断采用符合物理诠释的局域(或单点)平滑;又若大气非线性运动不稳定区恰是天气系统发源地,则全球多重/时变套网格双三次数值模式是今后研究的方向。

关键词：双三次数值模式全球经纬网格模式设计准拉格朗日/欧拉时间积分方案数值模拟大气非线性运动