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Proteomic response of Phaeocystis globosa to nitrogen limitation

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Journal of Oceanology and Limnology 2024年第1期42卷 141-149页

作者： Haisu LiU Ruiwang WEi Qiangyong lei lei CUi Songhui LÜ Key Laboratory of Eutrophication and Red Tide Prevention Research Center for Harmful Algae and Marine BiologyJinan UniversityGuangzhou 510632China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China National Engineering Laboratory of Electronic and information Standardization Shenzhen 518057China

Phaeocystis globosa is an important unicellular eukaryotic alga that can also form *** can cause massive harmful algal blooms and plays an important role in the global carbon or sulfur *** far,the ecophysiology of *** has been investigated by numerous ***,the proteomic response of *** to nitrogen depletion remains largely *** compared four protein preparation methods of *** for two-dimensional electrophoresis(2-DE)(Urea/Triton X-100 with trichloroacetic acid(TCA)/acetone precipitation;TCA/acetone precipitation;Radio immuno Precipitation Assay(RiPA)with TCA/acetone precipitation;and Tris buffer).Results show that the combination of RiPA with TCA/acetone precipitation had a clear gel background and showed the best protein spot separation effect,based on which the proteomic response to nitrogen depletion was studied using *** addition,we identified six differentially expressed proteins whose relative abundance increased or decreased more than 1.5-fold(Pins could not be identified,which might be attributed to the lack of genomic sequences of *** nitrogen limitation,replication protein-like,RNA ligase,and sn-glycerol-3-phosphate dehydrogenase were reduced,which may decrease the DNA replication level and ATP production in *** *** increase of endonucleaseⅢand transcriptional regulator enzyme may affect the metabolic and antioxidant function of *** cells and induce cell *** findings provide a basis for further proteomic study of *** and the optimization of protein preparation methods of marine microalgae.

关键词： Phaeocystis globosa nitrogen limitation proteomic response two-dimensional electrophoresis

Numerical analysis of a projecting wall type oscillating water column(PW-OWC)wave energy converter in regular waves

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Journal of Hydrodynamics 2024年第3期36卷 479-491页

作者： lei Tan Ruiyuan Chang Tomoki ikoma Department of Oceanic Architecture and Engineering College of Science and TechnologyNihon UniversityChibaJapan

Oscillating water column(OWC)based wave energy absorption devices are classic which have been widely used for harnessing ocean wave *** paper presents a numerical study on a projecting wall(PW)type OWC wave energy converter in regular *** computational fluid dynamics(CFD)modelling of a stationary floating PW-OWC model in a three-dimensional wave flume is achieved by the software *** analyses are carried out based on CFD simulations and the linear potential flow solutions with modifications to account for turbine-induced *** present numerical solutions are validated against our previous experimental *** is found that both the CFD and modified linear potential flow predictions are in reasonably good agreements with the experimental data in the first order results of OWC and air pressure *** the nonlinear responses are included in the result,the modified linear potential flow solution is found to slightly under-estimate the wave energy conversion performance at long *** the airflows above and below the chamber orifice,the CFD results suggest that they are almost unidirectional,oscillating in not only the base frequency but also subharmonic and ultraharmonic *** evolution of the OWC responses during an entire period and the phase analysis based on CFD simulations are *** phase results provide the crucial evidence to the reasonability of the physics-based modification of the potential flow model in modelling of *** present results and analysis are expected to be beneficial to the understanding on the physical mechanism of OWCs and the design of phase control strategies.

关键词： Computational fluid dynamics(CFD)simulation hydrodynamic analysis oscillating water column(OWC)damping orifice airflow phase difference

Progress and prospect of Pt-based catalysts for electrocatalytic hydrogen oxidation reactions

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Nano Research 2024年第3期17卷 960-981页

作者： Xu Zhang Ying Xie lei Wang Key Laboratory of Functional inorganic Material Chemistry Ministry of Education of the People’s Republic of ChinaHeilongjiang UniversityHarbin 150080China

To achieve the goals of the peak carbon dioxide emissions and carbon neutral,the development and utilization of sustainable clean energy are extremely *** fuel cells are an important system for converting hydrogen energy into electrical ***,the slow hydrogen oxidation reaction(HOR)kinetics under alkaline conditions has limited its ***,elucidating the catalytic mechanism of HOR in acidic and alkaline media is of great significance for the construction of highly active and stable *** terms of practicality,Pt is still the primary choice for commercialization of fuel *** the above basis,we first introduced the hydrogen binding energy theory and bifunctional theory used to describe the HOR activity,as well as the pH *** that,the rational design strategies of Pt-based HOR catalysts were systematically classified and summarized from the perspective of activity *** addition,we further emphasized the importance of theoretical simulations and in situ characterization in revealing the HOR mechanism,which is crucial for the rational design of ***,the practical application of Pt-based HOR catalysts in fuel cells was also *** closing,the current challenges and future development directions of HOR catalysts were *** review will provide a deep understanding for exploring the mechanism of highly efficient HOR catalysts and the development of fuel cells.

关键词： Pt-based catalysts hydrogen oxidation reaction(HOR) electrocatalytic mechanism hydrogen binding energy theory bifunctional theory

Sufficient Conditions for Amalgamated 3-Manifolds to be ∂-irreducible

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Chinese Annals of Mathematics,Series b 2024年第2期45卷 161-172页

作者： bing FANG Fengchun lei Liang LiANG School of Mathematical Sciences Dalian University of TechnologyDalian 116024LiaoningChina School of Mathematics Liaoning Normal UniversityDalian 116029LiaoningChina

in this paper,the authors give some sufficient conditions for an amalgamated 3-manifold along a compact connected surface F with boundary to be ∂-irreducible in terms of distances between some kinds of vertex subsets ... 详细信息

关键词： ∂-irreducibility irreducibility Amalgamated 3-manifold Curve complex Arc complex

Fault diagnosis of HVAC system with imbalanced data using multi-scale convolution composite neural network

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building Simulation 2024年第3期17卷 371-386页

作者： Rouhui Wu Yizhu Ren Mengying Tan lei Nie School of Mechanical Engineering Hubei University of TechnologyWuhan430068China industrial Research institute of Xiangyang Hubei University of Technology Xiangyang441100China

Accurate fault diagnosis of heating,ventilation,and air conditioning(HVAC)systems is of significant importance for maintaining normal operation,reducing energy consumption,and minimizing maintenance ***,in practical applications,it is challenging to obtain sufficient fault data for HVAC systems,leading to imbalanced data,where the number of fault samples is much smaller than that of normal ***,most existing HVAC system fault diagnosis methods heavily rely on balanced training sets to achieve high fault diagnosis ***,to address this issue,a composite neural network fault diagnosis model is proposed,which combines SMOTETomek,multi-scale one-dimensional convolutional neural networks(M1DCNN),and support vector machine(SVM).This method first utilizes SMOTETomek to augment the minority class samples in the imbalanced dataset,achieving a balanced number of faulty and normal ***,it employs the M1DCNN model to extract feature information from the augmented ***,it replaces the original Softmax classifier with an SVM classifier for classification,thus enhancing the fault diagnosis *** the SMOTETomek-M1DCNN-SVM method,we conducted fault diagnosis validation on both the ASHRAE RP-1043 dataset and experimental dataset with an imbalance ratio of 1:*** results demonstrate the superiority of this approach,providing a novel and promising solution for intelligent building management,with accuracy and F1 scores of 98.45%and 100%for the RP-1043 dataset and experimental dataset,respectively.

关键词： fault diagnosis chiller imbalanced data SMOTETomek multi-scale neural networks

Recent progress of cobalt-based electrocatalysts for water splitting: Electron modulation, surface reconstitution, and applications

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Nano Research 2024年第4期17卷 2234-2269页

作者： Zhijian Liang Di Shen lei Wang Honggang Fu Key Laboratory of Functional inorganic Material Chemistry Ministry of Education of the People’s Republic of ChinaHeilongjiang UniversityHarbin 150080China

Electrocatalytic water splitting is an essential and effective means to produce green hydrogen energy structures,so it is necessary to develop non-precious metal catalysts to replace precious ***-based catalysts present effective alternatives due to the diverse valence states,adjustable electronic structures,and plentiful *** this review,the catalytic mechanisms of hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)for electrocatalytic water splitting are ***,the synthesis strategies of various cobalt-based catalysts are systematically summarized,followed by the relationships between the structure and performance ***,the effects of d-band center and spin regulation for cobalt-based catalysts are also ***,the dynamic electronic and structural devolution of cobalt-based catalysts are elucidated by combining a series of in-situ ***,we highlight the challenges and future developed directions of cobalt-based catalysts for electrocatalytic water splitting.

关键词： cobalt-based electrocatalysts water splitting electron modulation surface reconfiguration

Vibration attenuation performance of wind turbine tower using a prestressed tuned mass damper under seismic excitation

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Earthquake Engineering and Engineering Vibration 2024年第2期23卷 511-524页

作者： lei Zhenbo Liu Gang Wang Hui Hui Yi School of Civil Engineering Chongqing UniversityChongqing 400045China Key Laboratory of New Technology for Construction of Cities in Mountain Area Chongqing UniversityMinistry of EducationChongqing 400045China

With the rapid development of large megawatt wind turbines,the operation environment of wind turbine towers(WTTs)has become increasingly *** particular,seismic excitation can create a resonance response and cause excessive vibration of the *** investigate the vibration attenuation performance of the WTT under seismic excitations,a novel passive vibration control device,called a prestressed tuned mass damper(PS-TMD),is presented in this ***,a mathematical model is established based on structural dynamics under seismic ***,the mathematical analytical expression of the dynamic coefficient is deduced,and the parameter design method is obtained by system tuning ***,based on a theoretical analysis and parameter design,the numerical results showed that the PS-TMD was able to effectively mitigate the resonance under the harmonic basal ***,the time-history analysis method is used to verify the effectiveness of the traditional pendulum tuned mass damper(PTMD)and the novel PS-TMD device,and the results indicate that the vibration attenuation performance of the PS-TMD is better than the *** addition,the PS-TMD avoids the nonlinear effect due to the large oscillation angle,and has the potential to dissipate hysteretic energy under seismic excitation.

关键词： wind turbine tower prestressed tuned mass damper vibration control seismic excitation numerical simulation

A semi-analytical model for coupled flow in stress-sensitive multi-scale shale reservoirs with fractal characteristics

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Petroleum Science 2024年第1期21卷 327-342页

作者： Qian Zhang Wen-Dong Wang Yu-Liang Su Wei Chen Zheng-Dong lei lei Li Yong-Mao Hao Key Laboratory of Unconventional Oil&Gas Development(China University of Petroleum(East China)) Ministry of EducationQingdao266580ShandongChina School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Shale Oil Development branch Changqing Oilfield CompanyPetroChinaQingyang745708GansuChina PetroChina Research institute of Petroleum Exploration&Development Beijing100083China

A large number of nanopores and complex fracture structures in shale reservoirs results in multi-scale flow of oil. With the development of shale oil reservoirs, the permeability of multi-scale media undergoes changes due to stress sensitivity, which plays a crucial role in controlling pressure propagation and oil flow. This paper proposes a multi-scale coupled flow mathematical model of matrix nanopores, induced fractures, and hydraulic fractures. in this model, the micro-scale effects of shale oil flow in fractal nanopores, fractal induced fracture network, and stress sensitivity of multi-scale media are considered. We solved the model iteratively using Pedrosa transform, semi-analytic Segmented bessel function, Laplace transform. The results of this model exhibit good agreement with the numerical solution and field production data, confirming the high accuracy of the model. As well, the influence of stress sensitivity on permeability, pressure and production is analyzed. it is shown that the permeability and production decrease significantly when induced fractures are weakly supported. Closed induced fractures can inhibit interporosity flow in the stimulated reservoir volume (SRV). it has been shown in sensitivity analysis that hydraulic fractures are beneficial to early production, and induced fractures in SRV are beneficial to middle production. The model can characterize multi-scale flow characteristics of shale oil, providing theoretical guidance for rapid productivity evaluation.

关键词： Multi-scale coupled flow Stress sensitivity Shale oil Micro-scale effect Fractal theory

Strong,tough,and thermally conductive nacre-inspired boron nitride nanosheet/epoxy layered nanocomposites

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Nano Research 2024年第2期17卷 820-828页

作者： Huagao Wang Rongjian Lu lei Li Cheng Liang Jia Yan Rui Liang Guoxing Sun lei Jiang Qunfeng Cheng School of Chemistry Key Laboratory of Bio-inspired Smart Interfacial Science and Technology of Ministry of EducationBeihang UniversityBeijing 100191China School of Chemistry and Materials Science University of Science and Technology of ChinaHefei 230026China Suzhou institute for Advanced Research University of Science and Technology of ChinaSuzhou 215123China Department of Stomatology Fifth Medical CenterChinese PLA General HospitalBeijing 100039China Department of Engineering Science Faculty of Innovation EngineeringMacao University of Science and TechnologyAvenida Wai LongTaipaMacao 999078 China Joint Key Laboratory of the Ministry of Education Institute of Applied Physics and Materials EngineeringUniversity of MacaoAvenida da UniversidadeTaipaMacao 999078China institute of Energy Materials Science(iEMS) University of Shanghai for Science and TechnologyShanghai 200093China

Thermally conductive polymer nanocomposites integrated with lightweight,excellent flexural strength,and high fracture toughness(Kic)would be of great use in many ***,achieving all of these properties simultaneously remains a great *** by natural nacre,here we demonstrate a lightweight,strong,tough,and thermally conductive boron nitride nanosheet/epoxy layered(bNNEL)*** of the layered structure and enhancing the interfacial interactions through hydrogen bonding and Si–O–b covalent bonding,the resulting nacre-inspired bNNEL nanocomposites show high fracture toughness of~4.22 MPa·m^(1/2),which is 7 folds as high as pure ***,the bNNEL nanocomposites demonstrate sufficient flexural strength(~168.90 MPa,comparable to epoxy resin),while also being lightweight(~1.23 g/cm^(3)).Additionally,the bNNEL nanocomposites display a thermal conductivity(κ)of~0.47 W/(m·K)at low boron nitride nanosheet loading of 2.08 vol.%,which is 2.7 times higher than that of pure epoxy *** developed nacre-inspired strategy of layered structure design and interfacial enhancement provides an avenue for fabricating high mechanical properties and thermally conductive polymer nanocomposites.

关键词： nacre-inspiration nanocomposites flexural strength fracture toughness(Kic) thermal conductivity