Two-year long field study was conducted using a permanent layout to investigate the economics of crop residues incorporation (2 t·ha-1) and P application (0, 40, 80 and 120 kg P2O5 ha-1) to directly sowing of ri...
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Two-year long field study was conducted using a permanent layout to investigate the economics of crop residues incorporation (2 t·ha-1) and P application (0, 40, 80 and 120 kg P2O5 ha-1) to directly sowing of rice and wheat crops gown under naturally salt-affected calcareous soil (ECe = 4.59 dS m-1;pHs = 8.38;SAR = 6.57 (mmolc L-1)1/2;CaCO3 = 3.21%;Extractable P = 4.07 mg·kg-1;sandy clay loam) at farmers field in district Hafizabad during the year 2012-13. Split plot design (crop residues in main plots and P application in sub plots) was followed with three replications. Agronomic data on growth and yield were collected at the time of each crop maturity. Maximum growth and yield of both the crops were harvested from the plots where P2O5 was applied @ 80 kg·ha-1 along with crop residues incorporation. On an average of two years, maximum paddy (3.26 t·ha-1) and wheat grain (3.56 t·ha-1) yield were produced with P application @ 80 kg P2O5 ha-1 along with crop residues incorporation. Although, the yield harvested with this treatment (80 kg P2O5 ha-1 + crop residues) performed statistically equal to 120 kg P2O5 ha-1 without crop residues incorporation during both the years, however, on an average of two years, grain yield of directly sowing rice and subsequent wheat was significantly superior (22% and 24% respectively) than that of higher P rate (120 kg·ha-1) without crop residues. Overall, continuous two-year crop residues incorporation further increased (17%) paddy yields during the follow up year of crop harvest. Economic analyses of both the crops were carried out to choose the best treatment with adequate economic benefits as compared to those without crop residue incorporation. Maximum net benefit of Rs = 108,680/- for direct seeded rice and Rs = 99,362/- for wheat grown with 80 kg P2O5 ha-1 application under crop residues incorporation was determined. Among P application treatments without crop residues incorporation, the maximu
Mycobacterium avium subspecies paratuberculosis(MAP) persistently survive and replicate in macrophages by adopting various strategies to subvert host immune response. Interleukin-10(IL-10) upregulated via MAPK-p38 sig...
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Mycobacterium avium subspecies paratuberculosis(MAP) persistently survive and replicate in macrophages by adopting various strategies to subvert host immune response. Interleukin-10(IL-10) upregulated via MAPK-p38 signaling cascade is a critical step for MAP survival and pathogenesis within the host cell. In the current study, we found that miR-27 a expression is downregulated during MAP infection both in vivo and in vitro. We observed that overexpression of miR-27 a inhibited MAP induced IL-10 production in macrophages and upregulated proinflammatory cytokines, while miR-27 a inhibitor counteracted these effects. Luciferase reporter assay results revealed that IL-10 and TAB2 are potential targets of miR-27 a. Furthermore, overexpression of miR-27 a significantly inhibited the intracellular survival of MAP in infected macrophages. Our data shows that miR-27 a augments antimicrobial activities of macrophages and inhibits the expression of IL-10, demonstrating that miR-27 a regulates protective innate immune responses during MAP infection and can be exploited as a novel therapeutic target in the control of intracellular pathogens, including MAP.
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