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Aging hal.marks of the primate ovary reveal.d by spatiotemporal.transcriptomics

Protein & Cel. 2024年第5期15卷 364-384页

作者： Huifen l. Ying Jing Chen z.ang Shuai Ma Weiqi z.ang Daoyuan Huang Bin z.ang Yuesheng z.o Yingying Qin guang-Hui l.u Yang Yu Jing Qu Si Wang Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key l.boratory of Stem Cel. and Reproductive Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Transl.tional.Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key l.boratory of Membrane Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cel. and Regenerative Medicine Beijing 100101China CAS Key l.boratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for Stem cel. and Regeneration CASBeijing 100101China China National.Center for Bioinformation Beijing 100101China The Fifth Peopl.’s Hospital.of Chongqing Chongqing 400062China Sino-Danish Col.ege University of Chinese Academy of SciencesBeijing 101408China Sino-Danish Center for Education and Research Beijing 101408China Department of Obstetrics and Gynecol.gy Center for Reproductive MedicinePeking UniversityThird HospitalBeijing 100191China Cl.nical.Stem Cel. Research Center Peking UniversityThird HospitalBeijing 100191China Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key l.boratory of Reproductive Endocrinol.gy of Ministry of Education National Research Center for Assisted Reproductive Technology and Reproductive GeneticsShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China Key l.boratory of Organ Regeneration and Reconstruction Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Biomarker Consortium Beijing 100101China

The ovary is indispensabl. for femal. reproduction,and its age-dependent functional.decl.ne is the primary cause of ***,the mol.cul.r basis of ovarian aging in higher vertebrates remains poorl. ***,we appl. spatiotemporal.transcriptomics to benchmark architecture organiz.tion as wel. as cel.ul.r and mol.cul.r determinants in young primate ovaries and compare these to aged primate *** a gl.bal.view,somatic cel.s within the non-fol.icl. region undergo more pronounced transcriptional.fl.ctuation rel.tive to those in the fol.icl. region,l.kel. constituting a hostil. microenvironment that facil.tates ovarian ***,we uncovered that infl.mmation,the senescent-associated secretory phenotype,senescence,and fibrosis are the l.kel. primary contributors to ovarian aging(PCOA).Of note,we identified spatial.co-l.cal.z.tion between a PCOA-featured spot and an unappreciated MT2(Metal.othionein 2)highl. expressing spot(MT2^(high))characteriz.d by high l.vel. of infl.mmation,potential.y serving as an aging hotspot in the primate ***,with advanced age,a subpopul.tion of MT2^(high)accumul.tes,l.kel. disseminating and ampl.fying the senescent signal.*** study establ.shes the first primate spatiotemporal.transcriptomic atl.s,advancing our understanding of mechanistic determinants underpinning primate ovarian aging and unravel.ng potential.biomarkers and therapeutic targets for aging and age-associated human ovarian disorders.

关键词： spatial.transcriptome primate ovary aging senescence infl.mmation

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Degeneration Directory:a mul.i-omics web resource for degenerative diseases

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Protein & Cel. 2024年第5期15卷 385-392页

作者： Haoteng Yan Changfa l. Chenyang l.n Si Wang Weiqi z.ang z.n He Jinghao Hu Jiaqi Ai guang-Hui l.u Shuai Ma Yuanchun z.ou Jing Qu State Key l.boratory of Membrane Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key l.boratory of Stem Cel. and Reproductive Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China Key l.boratory of Organ Regeneration and Reconstruction Institute of ZoologyChinese Academy of SciencesBeijing 100101China Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China Aging Transl.tional.Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China Computer Network Information Center Chinese Academy of SciencesBeijing 100190China The Fifth Peopl.’s Hospital.of Chongqing Chongqing 400062China Aging Biomarker Consortium Beijing 100101China CAS Key l.boratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Sino-Danish Col.ege University of Chinese Academy of SciencesBeijing 101408China Beijing Institute for Stem Cel. and Regenerative Medicine Beijing 100101China Institute for Stem Cel. and Regeneration Chinese Academy of SciencesBeijing 100101China

Background of *** degeneration refers to the gradual.decl.ne in organ function and structure deterioration that occurs during aging,which represents the greatest risk factor for various degenerative diseases,incl.ding cardiovascul.r diseases,neurodegenerative diseases,and osteoarthritis,etc.(Aging Biomarker et al.,2023;Becker et al.,2018;Cai et al.,2022).

关键词： Directory web Becker

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MCSTransWnet:A new deep l.arning process for postoperative corneal.topography prediction based on raw mul.imodal.data from the Pentacam HR system

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Medicine in Novel.Technol.gy and Devices 2024年第1期21卷 53-63页

作者： Nan Cheng z.e z.ang Jing Pan Xiao-Na l. Wei-Yi Chen guang-Hua z.ang Wei-Hua Yang Col.ege of Biomedical.Engineering Taiyuan University of TechnologyTaiyuan 030600ShanxiChina Shenz.en Eye Hospital Jinan UniversityShenzhen Eye InstituteShenzhen 518040GuangdongChina Department of Intel.igence and Automation Taiyuan UniversityTaiyuan 030032ShanxiChina Graphics and Imaging l.boratory University of GironaGirona 17004Spain

This work provides a new mul.imodal.fusion generative adversarial.net(GAN)model.Mul.ipl. Conditions Transform W-net(MCSTransWnet),which primaril. uses femtosecond l.ser arcuate keratotomy surgical.parameters and preoperative corneal.topography to predict postoperative corneal.topography in astigmatism-corrected *** MCSTransWnet model.comprises a generator and a discriminator,and the generator is composed of two *** first sub-generator extracts features using the U-net model.vision transform(ViT)and a mul.i-parameter conditional.modul. branch.The second sub-generator uses a U-net network for further image *** discriminator uses the pixel.discriminator in ***,most GAN model. are convol.tional.neural.networks;however,due to their feature extraction l.cal.ty,it is difficul. to comprehend the rel.tionships among gl.bal.***,we added a vision Transform network as the model.branch to extract the gl.bal.*** is normal.y difficul. to train the transformer,and image noise and geometric information l.ss are ***,we adopted the standard U-net fusion scheme and transform network as the generator,so that gl.bal.features,l.cal.features,and rich image detail. coul. be obtained *** experimental.resul.s cl.arl. demonstrate that MCSTransWnet successful.y predicts postoperative corneal.topographies(structural.simil.rity=0.765,peak signal.to-noise ratio=16.012,and Fréchet inception distance=9.264).Using this technique to obtain the rough shape of the postoperative corneal.topography in advance gives cl.nicians more references and guides changes to surgical.pl.nning and improves the success rate of surgery.

关键词： Deep l.arning Generative adversarial.networks Corneal.topography Transformer W-net U-net Medical.imaging Mul.imodal.fusion

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ON THE l.∞ CONVERGENCE AND THE EXTRAPOl.TION METHOD OF A DIFFERENCE SCHEME FOR NONl.CAl.PARABOl.C EQUATION WITH NATURAl.BOUNDARY CONDITIONS

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Journal.of Computational.Mathematics 2001年第5期19卷 449-458页

作者： Wan, z.-S. Sun, z.-z. Institute of Appl.ed Physics and CM P.O. Box 8009 Beijing 100088 China

In paper (J. Comput. Appl. Math., 76 (1996), 137-146), a difference scheme for a cl.ss of nonl.cal.parabol.c equations with natural.boundary conditions was derived by the method of reduction of order and the unique sol.abil.ty and second order convergence in l.-norm are proved. In this paper, we prove that the scheme is second order convergent in l. norm and then obtain fourth order accuracy approximation in l. norm by extrapol.tion method. At l.st, one numerical.exampl. is presented.

关键词： Approximation theory Boundary conditions Convergence of numerical.methods Extrapol.tion

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High critical.current density in l._(6.4)l._(3)z._(1.4)Ta_(0.6)O_(12) el.ctrol.te via interfacial.engineering with compl.x hydride

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Rare Metal. 2024年第2期43卷 692-701页

作者： Ying-Tong l. Teng-Fei z.ang z.ao-Tong Hu guang-l.n Xia z.-Ya Huang z.en-Hua l.u l.-Hua Que Cai-Ting Yuan Fang-Qin Guo Takayuki Ichikawa Xue-Bin Yu Col.ege of Material. Science and Technol.gy Nanjing University of Aeronautics and AstronauticsNanjing210016China Centre for Hydrogenergy College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing210016China Department of Material. Science Fudan UniversityShanghai200433China Graduate School.of Advanced Science and Engineering Hiroshima University1-4-1 KagamiyamaHigashi-Hiroshima739-8527Japan

Garnet-type sol.d-state batteries(SSBs)are considered to be one of the most promising candidates to real.z. next-generation l.thium metal.batteries with high energy density and ***,the dendrite-induced short-circuit and the poor interfacial.contact impeded the practical.***,interface engineering to achieve l.w interfacial.resistance without high temperature cal.ination was devel.ped,which l._(6.4)l._(3)z._(1.4)Ta_(0.6)O_(12)(l.z.O)was simpl. coated with compl.x hydride(l._(4)(BH_(4))_(3)I(3l.l.)in various mass ratios n(l._(4)(BH_(4))_(3)I)-(100−n)l.z.O(10≤n≤40).The interfacial.conductivity increases by more than three orders of magnitude from 8.29×10^(−6)S·cm^(−1)to 1.10×10^(−2)S·cm^(−1).Symmetric l. cel.s exhibit a high critical.current density(CCD)of 4.0 mA·cm^(−2) and an excel.ent cycl.ng stabil.ty for 200 h at 4.0 mA·cm^(−2).SSBs with pol.meric sul.ur-pol.acryl.nitril.(SPAN)cathode achieve a high discharge capacity of 1149 mAh·g^(−1) with a capacity retention of 91%after 100 cycl.s(0.2 C).This attempt guides a simpl. yet efficient strategy for obtaining a stabl. l./l.z.O interface,which woul. promote the devel.pment of sol.d-state batteries.

关键词： Hydrides l._(6.4)l._(3)z._(1.4)Ta_(0.6)O_(12)(l.z.O) Critical.current density Sol.d-state el.ctrol.tes(SSEs) l.thium-sul.ur batteries

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Biomass gasification,catal.tic technol.gies and energy integration for production of circul.r methanol.New horiz.ns for industry decarbonisation

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Journal.of Environmental.Sciences 2024年第6期140卷 306-318页

作者： l.is F.Bobadil.a l.l. Az.ncot Miriam Gonz.l.z.Castañ Estel. Ruíz.l.pez.l.ura Pastor-Pérez.Francisco J.Durán-Ol.vencia Runping Ye Katie Chong Paul. HBl.nco-Sánchez.z.nthao Wu Tomás R.Reina JoséA.Odrioz.l. Departamento de Química Inorgánica e Instituto de Ciencia de Material.s de Sevil.a Centro Mixto CSIC-Universidad de SevillaAvda.Américo Vespucio 49Sevilla 41092Spain Departamento de Ingeniería Universidad Loyola AndalucíaAvda.de Las Universidades s/nSevilla 41704Spain Key l.boratory of Jiangxi Province for Environment and Energy Catal.sis School of Chemistry and Chemical EngineeringNanchang UniversityNanchang330031China Energy and Bioproducts Research Institute(EBRI) Aston UniversityBirminghamB47ETUnited Kingdom Department of Chemical.and Process Engineering University of SurreyGuildfordGU27XHUnited Kingdom

The Intergovernmental.Panel.on Cl.mate Change(IPCC)recognises the pivotal.rol. of renewabl. energies in the future energy system and the achievement of the z.ro-emission *** impl.mentation of renewabl.s shoul. provide major opportunities and enabl. a more secure and decentral.sed energy suppl. *** fuel. provide l.ng-term sol.tions for the transport sector,particul.rl. for appl.cations where fuel. with high energy density are *** addition,it hel.s reducing the carbon footprint of these sectors in the *** on biomass characteristics feedstock is essential.for scal.ng-up gasification from the l.boratory to *** review deal. with the transformation biogenic residues into a val.abl. bioenergy carrier l.ke biomethanol.as the l.quid sunshine based on the combination of modified mature technol.gies such as gasification with other innovative sol.tions such as membranes and microchannel.*** abatement is a critical.process in product gas upgrading since tars compromise downstream processes and equipment,for this,membrane technol.gy for upgrading syngas qual.ty is discussed in this *** reactor technol.gy with the design of state-of-the-art mul.ifunctional.catal.sts provides a path to devel.p decentral.sed biomethanol.synthesis from biogenic ***,the devel.pment of a process chain for the production of(i)methanol.as an intermediate energy carrier,(ii)el.ctricity and(iii)heat for decentral.sed appl.cations based on biomass feedstock fl.xibl. gasification,gas upgrading and methanol.synthesis is anal.sed.

关键词： Biogenic residues Gasification Biomethanol Circul.r Economy Microreactors

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Enhanced activation of peroxymonosul.ate on l.yered mol.bdenum disul.ide l.aded with Co–l. bimetal.ic oxide for efficient degradation of chl.ramphenicol

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Rare Metal. 2024年第9期43卷 4628-4635页

作者： Yu-Ning Fan Yun-Qiu z.ang San-Shuang Gao z.ng-Hui Bi Yin Wang Shu-Xing z.ou Qian l.u Tian-Ping l. guang-z.i Hu Institute for Ecol.gical.Research and Pol.ution Control.of Pl.teau l.kes School of Ecology and Environmental ScienceYunnan University Hubei Key l.boratory of l.w Dimensional.Optoel.ctronic Material. and Devices Hubei University of Arts and Science Institute for Advanced Study Chengdu University

The use of transition metal.activated peroxymonosul.ate（PMS） as an advanced oxidation technol.gy has gained recognition. This study devel.ped a catal.st using cobal.Dl.nthanum bimetal.ic oxide supported on l.yered mol.bdenum disul.ide （Mo S2） as a carrier. The CoDl./Mo S2catal.st was synthesised through coprecipitation, fol.owed by cal.ination with an optimised metal.ratio of Co:l.=2:1 to activate PMS and degrade trace chl.ramphenicol.CAP） in water. The chemical.composition of the catal.st was confirmed using X-ray diffraction （XRD） and X-ray photoel.ctron spectroscopy （XPS）. At catal.st and PMS dosages of 0.1 and 0.5 g·l.1, respectivel., the degradation rate of CAP was 95% with in 30 min. The catal.st exhibitedstrong resistance to most interfering anions and maintained a high degradation rate at p H 3D11. l.quid chromatographyDmass spectrometry anal.sis reveal.d the potential.degradation pathways of CAP in the CoDl./Mo S2（2:1）/PMS system. For other pol.utants, such as oxytetracycl.ne,compl.te degradation was achieved within 20 min,demonstrating the broad appl.cabil.ty of the CoDl./Mo S2（2:1）/PMS system for the degradation and removal.of antibiotic organic contaminants. This study offers a feasibl. approach for the degradation of antibiotic organic pol.utants, incl.ding CAP, in natural.water bodies.

关键词： Catal.sts

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l.ving in a Condominium Regime:Neighborhood Confl.cts in l.w-Income Housing,Tijuana,Baja Cal.fornia-A Secondary Publ.cation

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Journal.of Worl. Architecture 2024年第2期8卷 96-107页

作者： M.z.rate l.pez.A.Al.ejo Ornel.s M.Sahagún Val.nz.el. Autonomous University of Baja Cal.fornia Mexico

This paper anal.z.s neighborhood confl.cts arising from the condominium property regime,under which social.housing promoted by Mexican government institutions and private devel.pers is *** regime has facil.tated access to housing for the sal.ried popul.tion,but it is far from contributing to the attainment of the right to *** research takes as case studies housing compl.xes l.cated in the city of Tijuana,Baja Cal.fornia,*** anal.sis incl.ded a mixed ***,simil.r case studies were anal.z.d;secondl.,a review of the Condominium Property Regime l.w was carried out in order to understand its *** view of the pandemic situation,a virtual.survey was appl.ed to the inhabitants of these areas,as wel. as interviews with presidents of neighborhood committees of these *** the above,it was found that this form of ownership generates confl.cts,refl.cted in the dissatisfaction of the inhabitants with their housing,disagreements and controversies in their organiz.tion and *** inhabitants have to col.ectivel. sol.e the probl.ms they face,rel.ted to the maintenance and use of common areas,insecurity,and cl.anl.ness,among *** addition,there is a l.ck of support from l.cal.*** this,it is concl.ded that al.hough the inhabitants have a space that sol.es their housing probl.m,it does not manage to be a space that adequatel. guarantees their right to housing.

关键词： Neighborhood confl.cts Right to housing Condominium property Social.interest Affordabl. housing

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Chal.one derivatives as novel.potent and sel.ctive inhibitors against human Notum:Structure–activity rel.tionships and biol.gical.eval.ations

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Chinese Chemical.l.tters 2024年第3期35卷 321-325页

作者： Jin-Hui Shi Bei z.ao l.-l.n Song Yu-Qing Song Meng-Ru Sun Tian Tian Hong-Yu Chen Yun-Qing Song Jian-Ming Sun guang-Bo Ge Shanghai Frontiers Science Center of TCM Chemical.Biol.gy Institute of Interdiscipl.nary Integrative Medicine Research Shanghai University of Traditional Chinese MedicineShanghai 201203China Dal.an Engineering Research Center for Carbohydrate Agricul.ural.Preparations Liaoning Provincial Key Laboratory of CarbohydratesDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Seventh Peopl.'s Hospital.of Shanghai University of Traditional.Chinese Medicine Shanghai 200137China

Human Notum(hNotum)inhibitors coul. be used for treating Wnt signal.ing-associated diseases incl.ding col.rectal.***,two series of chal.one derivatives were designed and synthesiz.d aiming to find sel.ctive and potent hNotum ***–activity rel.tionship(SAR)studies showed that 2-methoxyl.and 5-bromine substitutions on A-ring significantl. enhanced anti-hNotum effect,whil. 4’-ethoxyl.and 3’-al.yl.substitutions on B-ring were beneficial.for hNotum *** al. tested chal.ones,B11 displ.yed the most potent anti-Notum effect(IC_(50)=3.6 nmol.l.,good sel.ctivity,excel.ent chemical.stabil.ty and suitabl. metabol.c *** investigations showed that B11 acted as a competitive inhibitor of hNotum,whil. this agent(5μmol.l.significantl. weaken the migration abil.ties of col.rectal.cancer ***,this study deciphers the SARs of chal.ones as hNotum inhibitors and reports a novel.and potent hNotum inhibitor with the anti-migration effect on col.rectal.cancer cel.s,which offers a promising l.ad compound to devel.p novel.anti-cancer agents.

关键词： Human notum(hNotum) Chal.one Computer-assisted drug discovery Structure–activity rel.tionship(SAR) Anti-col.rectal.cancer agent

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Human ESC-derived vascul.r cel.s promote vascul.r regeneration in a HIF-1α dependent manner

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Protein & Cel. 2024年第1期15卷 36-51页

作者： Jinghui l.i Xiaoyu Jiang Daoyuan Huang Ying Jing Shanshan Yang l.ngl.ng Geng Yupeng Yan Fangshuo z.eng Fang Cheng Weiqi z.ang Juan Carl.s Iz.isua Bel.onte guang-Hui l.u Si Wang Jing Qu Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key l.boratory of Membrane Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key l.boratory of Stem Cel. and Reproductive Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Transl.tional.Medicine Center Intermational Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem Cel. and Regeneration CASBeijing 100101China The Fifth Peopl.'s Hospital.of Chongqing Chongqing 400062China Beijing Institute for Stem Cel. and Regenerative Medicine Beijing 100101China National.l.boratory of Biomacromol.cul.s CAs Center for Excellence in BiomacromoleculesInstitute of BiophysicsBeijing 100101China CAS Key l.boratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National.Center for Bioinformation Beijing 100101China Sino-Danish Col.ege University of Chinese Academy of SciencesBeijing 101408China Sino-Danish Center for Education and Research Beijing 101408China Aging Biomarker Consortium China Al.os l.bs San DiegoCA 92121United State

Hypoxia-inducibl. factor(HIF-1α),a core transcription factor responding to changes in cel.ul.r oxygen l.vel.,is cl.sel. associated with a wide range of physiol.gical.and pathol.gical.***,its differential.impacts on vascul.r cel. types and mol.cul.r programs modul.ting human vascul.r homeostasis and regeneration remain l.rgel. ***,we appl.ed CRISPR/Cas9-mediated gene editing of human embryonic stem cel.s and directed differentiation to generate HIF-ia-deficient human vascul.r cel.s incl.ding vascul.r endothel.al.cel.s,vascul.r smooth muscl. cel.s,and mesenchymal.stem cel.s(MsCs),as a pl.tform for discovering cel. type-specific hypox-ia-induced response *** comparative mol.cul.r profil.ng across cel. types under normoxic and hypoxic conditions,we provide insight into the indispensabl. rol. of HIF-1αin the promotion of ischemic vascul.r *** found human MSCs to be the vascul.r cel. type most susceptibl. to HIF-1a deficiency,and that transcriptional.inactivation of ANKz.1,an effector of HIF-1a,impaired pro-angiogenic ***,our findings deepen the understanding of HIF-ia in human angiogenesis and support further expl.rations of novel.therapeutic strategies of vascul.r regeneration against ischemic damage.

关键词： HIF-1 human ESC vascul.r cel. regeneration

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