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艾灸对佐剂性关节炎大鼠滑膜组织Beclin-1、LC3-Ⅱ表达的影响

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湖南中医药大学学报 2024年第1期44卷 122-127页

作者：彭雄周兰施雨燕龚志贤张亮艾坤李鑫陈青刘梨湖南中医药大学第一附属医院湖南长沙410007 湖南中医药大学湖南长沙410208 湖南中医药大学中医诊断学湖南省重点实验室湖南长沙410208

目的观察艾灸对类风湿关节炎(rheumatoid arthritis,RA)大鼠炎症因子白细胞介素-2(interleukin-2,IL-2)及滑膜细胞中自噬相关因子Beclin-1、微管相关蛋白1轻链3-Ⅱ(microtubule-associated protein1 light chain 3-Ⅱ,LC3-Ⅱ)表达的影响,探索艾灸治疗RA的作用机制。方法将36只雄性SD大鼠随机分为空白组、模型组、甲氨蝶呤组、艾灸组,每组9只。采用弗氏完全佐剂造模法制备RA大鼠模型。造模成功后艾灸组予艾灸足三里、关元,每次20 min,每天1次;甲氨蝶呤组予甲氨蝶呤0.35 mg/kg灌胃,每周2次。空白组、模型组、甲氨蝶呤组每天给予艾灸组大鼠同样时长及强度的捆绑。每组均干预3周。观察大鼠一般情况,采用足趾容积测量仪检测大鼠左后肢足趾容积,ELISA法检测血清中IL-2含量,Western blot检测大鼠踝关节滑膜组织中Beclin-1、LC3-Ⅱ蛋白相对表达量。结果与模型组比较,甲氨蝶呤组及艾灸组精神一般,反应尚可,体质量恢复,较活跃,摄食、饮水尚可,足部肿胀、红肿缓解,其局部炎症及全身多发性关节炎情况均轻于模型组。与空白组比较,模型组大鼠于造模后第3、10、17、24天足趾容积明显增大(P<0.01),结合一般情况,提示模型制备成功;甲氨蝶呤组、艾灸组足趾容积于第24天增大(P<0.05)。与模型组比较,甲氨蝶呤组造模后第17、24天足趾容积降低(P<0.05,P<0.01),艾灸组第10、17、24天足趾容积明显降低(P<0.01)。干预3周后,与空白组比较,模型组血清中IL-2含量明显升高(P<0.01),滑膜组织Beclin-1、LC3-Ⅱ蛋白表达量明显上升(P<0.01);与模型组比较,甲氨蝶呤组、艾灸组血清中IL-2含量降低(P<0.05),艾灸组滑膜组织Beclin-1、LC3-Ⅱ蛋白表达量下降(P<0.05)。结论艾灸能改善RA大鼠关节肿胀,降低IL-2含量,其作用机制可能是通过调节自噬因子Beclin-1、LC3-Ⅱ蛋白表达量有关。

关键词：类风湿关节炎佐剂性关节炎滑膜组织艾灸白细胞介素-2 Beclin-1 微管相关蛋白1轻链3-Ⅱ

血液外泌体来源miRNA对类风湿关节炎诊断价值的Meta分析

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标记免疫分析与临床 2024年第1期31卷 88-93页

作者：施雨燕龚志贤李鑫张亮王文怡徐美丽艾坤刘梨湖南中医药大学第一附属医院湖南长沙410007 湖南中医药大学中医诊断学湖南省重点实验室湖南长沙410208 湖南中医药大学湖南长沙410208

目的有研究发现血液外泌体(Exosomes)中的miRNA(microRNAs)在类风湿关节炎患者中存在异常表达,这可能成为RA诊断的潜在标志物。本研究的目的是系统评价血液外泌体来源的miRNA对类风湿关节炎(RA)的诊断价值。方法利用计算机收集PubMed... 详细信息

目的有研究发现血液外泌体(Exosomes)中的miRNA(microRNAs)在类风湿关节炎患者中存在异常表达,这可能成为RA诊断的潜在标志物。本研究的目的是系统评价血液外泌体来源的miRNA对类风湿关节炎(RA)的诊断价值。方法利用计算机收集PubMed、Web of science、Embase、Cochrane Library、中国知网、万方、维普网、中国生物医学文献等数据库中有关外泌体诊断类风湿关节炎方面的文献,根据制定的文献检索策略筛选文献并提取文献中相关数据。采用诊断准确性研究的质量评价工具(quality assessment of diagnostic accuracy studies, QUADS-2)对纳入文献进行质量评价。应用RevMan 5.3、Meta-Disc 1.4软件与Stata 16.0软件进行Meta分析,计算合并灵敏度、特异性、阳性似然比、阴性似然比、诊断比值比等指标,利用受试者工作特征(ROC)曲线及曲线下面积(AUC)对其异质性与发表偏倚进行分析。结果计算机共检索出2 698篇文献,经过筛选后纳入文献共6篇,其中包含18种miRNA,Meta分析结果显示不存在由阈值效应引起的异质性,但存在由非阈值效应引起的异质性,故采用随机效应模型合并效应量,合并灵敏度为0.80(95%CI 0.73~0.86)、合并特异性为0.77(95%CI 0.73~0.80)、合并阳性似然比为3.5(95%CI 2.9~4.2)、合并阴性似然比为0.26(95%CI 0.18~0.36)、合并诊断比值比为14(95%CI 8~22)、受试者工作特征(ROC)曲线下面积AUC=0.82(95%CI 0.79~0.85)。结论血液外泌体来源miRNA对类风湿关节炎(rheumatoid arthritis, RA)有较高诊断价值与意义,可作为诊断RA的新生标志物,但受目前文献数量及质量的限制,今后需要更多的临床研究加以验证。

关键词：外泌体类风湿关节炎诊断 Meta分析

Repurposing diacerein to suppress colorectal cancer growth by inhibiting the DCLK1/STAT3 signaling pathway

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Chinese Journal of Natural Medicines 2024年第4期22卷 318-328页

作者： YE Qiaobei ZHU Yu shi Meng LV Linxi GONG yuyan ZHANG Luyao YANG Lehe ZHAO Haiyang ZHAO Chengguang XU Huanhai Affiliated Yueqing Hospital Wenzhou Medical UniversityWenzhou 325600China The Institute of Life Sciences Biomedical Collaborative Innovation Center of Zhejiang ProvinceWenzhou UniversityWenzhou 325035China School of Pharmaceutical Sciences Wenzhou Medical UniversityWenzhou 325035China

Double cortin-like kinase 1(DCLK1)exhibits high expression levels across various cancers,notably in human colorectal cancer(CRC).Diacerein,a clinically approved interleukin(IL)-1βinhibitor for osteoarthritis treatment,was evaluated for its impact on CRC proliferation and migration,alongside its underlying mechanisms,through both in vitro and in vivo *** study employed MTT assay,colony formation,wound healing,transwell assays,flow cytometry,and Hoechst 33342 staining to assess cell proliferation,migration,and ***,proteome microarray assay and western blotting analyses were conducted to elucidate diacerein’s specific mechanism of *** findings indicate that diacerein significantly inhibits DCLK1-dependent CRC growth in vitro and in *** high-throughput proteomics microarray and molecular docking studies,we identified that diacerein directly interacts with ***,the suppression of p-STAT3 expression following DCLK1 inhibition by diacerein or specific DCLK1 siRNA was ***,diacerein effectively disrupted the DCLK1/STAT3 signaling pathway and its downstream targets,including MCL-1,VEGF,and survivin,thereby inhibiting CRC progression in a mouse model,thereby inhibiting CRC progression in a mouse model.

关键词： DCLK1 Diacerein Rhein STAT3 Colorectal cancer

Shear-wave Splitting in the Crust beneath Shandong and Its Adjacent Area

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Earthquake Research in China 2016年第4期30卷 556-565页

作者： Miao Qingjie Liu Xiqiang shi yuyan Qu Junhao Zheng Jianchang Tian Fengdong Earthquake Administration of Shandong Province Jinan 250102China

Based on the seismic data of near-field source from the Shandong Digital Seismic Network,this study obtained the shear-wave splitting parameters from multiple stations. The results show that dominant directions of polarizations of fast shear-waves reflect the spatial distribution characteristics of tectonic stress in this area,CHD and LIS stations show 2 dominant directions,and reveals that the crustal seismic anisotropy in the Shandong area is constrained by the regional stress background,local failure and structure.

关键词：剪切波分裂山东地区地壳邻区数字地震台网空间分布特征地震各向异性地震资料

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信息扩散函数在半参数地壳形变分析中的应用

大地测量与地球动力学 2023年第6期43卷 646-650页

作者：石雨燕王靖予杨鹏张俊贵州大学矿业学院贵阳市550025

提出一种利用信息扩散函数构建正则矩阵的新方法。首先,仅保留半参数模型的参数化模型部分,利用最小二乘估计获取参数模型的最小二乘残差;然后,将此残差向量作为信号,计算信号的信息扩散函数估值;最后,将信息扩散估计值作为正则矩阵的... 详细信息

提出一种利用信息扩散函数构建正则矩阵的新方法。首先,仅保留半参数模型的参数化模型部分,利用最小二乘估计获取参数模型的最小二乘残差;然后,将此残差向量作为信号,计算信号的信息扩散函数估值;最后,将信息扩散估计值作为正则矩阵的主对角元素构建正则矩阵。与时间序列法相比,新方法淡化了对信号连续性和光滑性的要求,且信息扩散函数估值本质上是残差的概率分布,相当于事先合理地确定了信号权阵,使得参数估计具有良好的稳健性。中国大陆华北块体的半参数地壳形变分析结果表明,新方法具有明显优势。

关键词：地壳形变分析信息扩散函数半参数模型正则矩阵

Economic Value of Water Quality Improvement by One Grade Level in Erhai Lake： A Willingness-to-Pay Survey and a Benefit-Transfer Study

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Frontiers of Economics in China-Selected Publications from Chinese Universities 2015年第1期10卷 168-199页

作者： Hua Wang yuyan shi Yoonhee Kim Takuya Kamata Policy Research Center for Environment and Economy Ministry of Environmental ProtectionBeijing 100029 China Department of Family Medicine and Public Health University of California San DiegoCA92093 USA Development Research Group The World Bank Washington DC 20433 USA

Water quality in China has seriously deteriorated in recent years. However, very few valuation studies have been conducted to estimate the monetary values associated with water quality changes. As a result, the decision makers can hardly make rational choices with regard to investments in water quality improvement. This paper presents a valuation study conducted in Dali, Yunnan Province, which aims to estimate the total economic value of improving the water quality of Erhai Lake by one grade level. Both the contingent valuation method and the benefit transfer approach are employed in this study. The contingent valuation estimation strategy reveals that, on average, a household located in Dali is willing to pay about 27 yuan per month continuously for 5 years for the water quality improvement, equivalent to 1.7% of the household monthly income. The elasticity of willingness-to-pay with respect to income is estimated to be 0.28. The internal rate of economic return of the proposed pollution control project is estimated to be 13%. The benefit transfer exercise produces a similar estimation on willingness to pay （WTP） values, with a difference of less than 2% compared with the contingent valuation approach. The results indicate the potential reliability of using the benefit transfer approach for valuation estimations in Chinese provinces.

关键词： contingent valuation benefit transfer water quality

2022-01-15汤加火山喷发的地震学震级测定

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大地测量与地球动力学 2023年第2期43卷 209-214页

作者：石玉燕张春鹏颜启苗庆杰山东省地震局济南市250014

利用山东地震台网70个宽频带测震台站记录的2022-01-15南太平洋汤加海底火山喷发原始地震波形数据,从时间域和频率域分析火山喷发地震波的记录特征;通过对20 s优势周期面波进行研究,测算该次火山喷发的地震学震级与地震波辐射能量。研... 详细信息

利用山东地震台网70个宽频带测震台站记录的2022-01-15南太平洋汤加海底火山喷发原始地震波形数据,从时间域和频率域分析火山喷发地震波的记录特征;通过对20 s优势周期面波进行研究,测算该次火山喷发的地震学震级与地震波辐射能量。研究表明,该次汤加火山喷发的面波震级为M_(S)5.674、能量震级为M_(e)5.704,与GFZ测定震级基本一致,比USGS测定震级小0.126。本次汤加火山爆炸指数(VEI)为5级,但本文测定的地震波辐射能量为8.155×10^(12)J,可见火山喷发以地震波辐射方式释放的能量较少,绝大部分能量以超过1000 km/h的冲击波形式穿过大气层迅速释放到空中,这种能量的快速释放会抑制深部应力的挤压与拉伸。

关键词：火山喷发面波震级台基校正时频分析地震波辐射能量

Motor symptom machine rating system for complete MDS-UPDRS III in Parkinson’s disease:A proof-of-concept pilot study

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Chinese Medical Journal 2024年第13期137卷 1632-1634页

作者： Xue Zhu Zhonglue Chen Yun Ling Ningdi Luo Qianyi Yin Yichi Zhang Aonan Zhao Guanyu Ye Haiyan Zhou Jing Pan Liche Zhou Linghao Cao Pei Huang Pingchen Zhang Cheng Chen Weikun shi shinuan Lin Haimei Zhuang Jin Zhao Kang Ren yuyan Tan Jun Liu Department of Neurology and Institute of Neurology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China GYENNO Science Co. Ltd.Nanshan DistrictShenzhenGuangdong 518100China HUST-GYENNO CNS Intelligent Digital Medicine Technology Center WuhanHubei 430074China School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhanHubei 430074China CAS Center for Excellence in Brain Science and Intelligence Technology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

To the Editor:Parkinson’s disease(PD)is one of the most common neurodegenerative movement disorders.[1]The severity of PD-related motor symptoms is usually semiquantitatively(“normal”,“slight”,“mild”,“moderate”,and“severe”)evaluated by expert physicians according to the Movement Disorder Society-sponsored revision of the Unified Parkinson’s Disease Rating Scale Part III(MDS-UPDRS III).[2]However,the MDS-UPDRS III is semiquantitative and subjective,which might mask mild treatment effects or even provide false-positive results.

关键词： MDS proof severity

Discussion on the Measurement Method for the Coda Wave Quality Factor

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Earthquake Research in China 2009年第4期23卷 420-433页

作者： Liu Xiqiang shi yuyan Qu Junhao Li Hong Li Bo Zhang Kun Earthquake Administration of Shandong Province Jinan 250014 China

The Quality factor is the parameter that can be used to describe the energy attenuation on seismic wave. In theory, we can obtain the relationship between the change of the coda wave quality factor with time and the strong earthquake preparation process on the basis of the quality factor of a coda wave in a same ray path. However, in reality the coda wave quality factor measured by different seismic coda waves corresponds to different seismic wave ray paths. The change of the quality factor with time is related to non-elastic characteristics of the medium and the volume of scattering ellipsoid constrained by scattered wave phase fronts, besides the change of regional stress field. This paper discusses the relationship between quality factor, epicenter distance and different lapse time, and then discusses the relationship between quality factor and frequency. Furthermore the determination method of the coda wave quality factor is put forward. The improved determination method of the quality factor, which removes the influence of different earthquakes or propagation depth of scattered waves, may increase measurement precision, thus information pertaining to abnormal changes in quality factor and the relationship between the quality factor and earthquake preparation process can be acquired.

关键词：品质因数测量方法尾波地震波能量质量因素品质因子地震孕育过程时间变化