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6-Phosphogluconate dehydrogenase 2 bridges the OPP and shikimate pathways to enhance aromatic amino acid production in plants

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Science China Life Sciences 2024年

作者： Qian Tang yuxin huang Zhuanglin Shen Linhui Sun Yang Gu Huiqing He Yanhong Chen Jiahai Zhou Limin Zhang Cuihuan Zhao Shisong Ma Yunhai Li Jie Wu Qiao Zhao Shenzhen Key Laboratory of Synthetic Genomics Guangdong Provincial Key Laboratory of Synthetic Genomics Key Laboratory of Quantitative Synthetic Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced Technology Chinese Academy of Sciences University of Chinese Academy of Sciences CAS Key Laboratory for Quantitative Engineering Biology Shenzhen Institute of Synthetic Biology Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Key Laboratory of Quantitative Synthetic Biology Shenzhen Institute of Synthetic Biology Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Key Laboratory of Agricultural Microbiology of Heilongjiang Province Northeast Agricultural University Center for Plant Biology School of Life Sciences Tsinghua University State Key Laboratory of Plant Cell and Chromosome Engineering CAS Centre for Excellence in Molecular Plant Biology Institute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics School of Life Sciences University of Science and Technology of China Innovation Academy for Seed Design Chinese Academy of Sciences

The oxidative pentose phosphate(OPP) pathway provides metabolic intermediates for the shikimate pathway and directs carbon flow to the biosynthesis of aromatic amino acids(AAAs), which serve as basic protein building blocks and precursors of numerous metabolites essential for plant growth. However, genetic evidence linking the two pathways is largely unclear. In this study, we identified 6-phosphogluconate dehydrogenase 2(PGD2), the rate-limiting enzyme of the cytosolic OPP pathway, through suppressor screening of arogenate dehydrogenase 2(adh2) in Arabidopsis. Our data indicated that a single amino acid substitution at position 63(glutamic acid to lysine) of PGD2 enhanced its enzyme activity by facilitating the dissociation of products from the active site of PGD2, thus increasing the accumulation of AAAs and partially restoring the defective phenotype of adh2. Phylogenetic analysis indicated that the point mutation occurred in a well-conserved amino acid residue. Plants with different amino acids at this conserved site of PGDs confer diverse catalytic activities, thus exhibiting distinct AAAs producing capability. These findings uncover the genetic link between the OPP pathway and AAAs biosynthesis through PGD2. The gain-of-function point mutation of PGD2 identified here could be considered as a potential engineering target to alter the metabolic flux for the production of AAAs and downstream compounds.

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CeO_(2)nanoparticles-decorated CoP nanocubes for accelerating alkaline electrocatalytic oxygen evolution reaction

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Particuology 2023年第10期81卷 38-44页

作者： Xueda Ding yuxin huang Dongyang Chen Zailai Xie College of Chemistry Fuzhou UniversityFuzhou350116China College of Materials Science and Engineering Fuzhou UniversityFuzhou350116China

Constructing heterojunction interface as an active catalyst is an effective strategy to boost electrocatalytic activity of oxygen evolution reaction(OER).Herein,we report an interfacial CoP/CeO_(2)heterostructure catalyst constructed by interface engineering and selective phosphorization procedure.X-ray photoelectron spectroscopy(XPS)suggests that coupling CeO_(2)nanoparticles on the surface of CoP will generate interfacial interaction at the two-phase interface,resulting in electron transfer between CoP and CeO_(2)components at the interface.Benefitting from the interfacial interaction,large exposed interface area,and luxuriant mesopores structure,CoP/CeO_(2)shows fascinating alkaline OER performance.At the current densities of 10 and 50 mA cm^(−2),the optimal CoP/CeO_(2)heterojunction exhibits lower overpotential(257 and 298 mV)than either CoP(288 and 354 mV)or RuO_(2)(305 and 409 mV).This work provides a facile synthetic protocol for constructing heterostructure interfaces to improve OER performance.

关键词： Prussian blue analogue CeO_(2)nanoparticles CoP/CeO_(2) Oxygen evolution reaction

Exploiting comments information to improve legal public opinion news abstractive summarization

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Frontiers of Computer Science 2022年第6期16卷 31-40页

作者： yuxin huang Zhengtao YU Yan XIANG Zhiqiang YU Junjun GUO Faculty of Information Engineering and Automation Kunming University of Science and TechnologyKunming650500China Yunnan Key Laboratory of Artificial Intelligence Kunming University of Science and TechnologyKunming650500China

Automatically generating a brief summary for legal-related public opinion news(LPO-news,which contains legal words or phrases)plays an important role in rapid and effective public opinion disposal.For LPO-news,the critical case elements which are significant parts of the summary may be mentioned several times in the reader comments.Consequently,we investigate the task of comment-aware abstractive text summarization for LPO-news,which can generate salient summary by learning pivotal case elements from the reader comments.In this paper,we present a hierarchical comment-aware encoder(HCAE),which contains four components:1)a traditional sequenceto-sequence framework as our baseline;2)a selective denoising module to filter the noisy of comments and distinguish the case elements;3)a merge module by coupling the source article and comments to yield comment-aware context representation;4)a recoding module to capture the interaction among the source article words conditioned on the comments.Extensive experiments are conducted on a large dataset of legal public opinion news collected from micro-blog,and results show that the proposed model outperforms several existing state-of-the-art baseline models under the ROUGE metrics.

关键词： legal public opinion news abstractive summarization comment comment-aware context case elements bidirectional attention

Linguistic feature template integration for Chinese-Vietnamese neural machine translation

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Frontiers of Computer Science 2022年第3期16卷 223-225页

作者： Zhiqiang YU Yantuan XIAN Zhengtao YU yuxin huang Junjun GUO Faculty of Information Engineering and Automation Kunming University of Science and TechnologyKunming 650500China School of Mathematics and Computer Science Yunnan Minzu UniversityKunming 650500China Yunnan Key Laboratory of Artificial Intelligence Kunming University of Science and TechnologyKunming 650500China

1 Introduction and main contributions Template-based approaches have achieved significant progress in low-resource neural machine translation(NMT)recently[1],such as the efficient works,NMT-GTM[2],SoftPrototype[3],etc.However,most previous works only retrieve target sentence as template to generate translation,neglecting the utilization of linguistic feature that contained in the source sentence and template.

关键词： translation template such

基于多策略强化学习的低资源跨语言摘要方法研究

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计算机工程 2024年第2期50卷 68-77页

作者：冯雄波黄于欣赖华高玉梦昆明理工大学信息工程与自动化学院云南昆明650504 昆明理工大学云南省人工智能重点实验室云南昆明650504

(CLS)旨在给定1个源语言文件(如越南语),生成目标语言(如中文)的摘要。端到端的CLS模型在大规模、高质量的标记数据基础上取得较优的性能,这些标记数据通常是利用机器翻译模型将单语摘要语料库翻译成CLS语料库而构建的。然而,由于低资... 详细信息

(CLS)旨在给定1个源语言文件(如越南语),生成目标语言(如中文)的摘要。端到端的CLS模型在大规模、高质量的标记数据基础上取得较优的性能,这些标记数据通常是利用机器翻译模型将单语摘要语料库翻译成CLS语料库而构建的。然而,由于低资源语言翻译模型的性能受限,因此翻译噪声会被引入到CLS语料库中,导致CLS模型性能降低。提出基于多策略的低资源跨语言摘要方法。利用多策略强化学习解决低资源噪声训练数据场景下的CLS模型训练问题,引入源语言摘要作为额外的监督信号来缓解翻译后的噪声目标摘要影响。通过计算源语言摘要和生成目标语言摘要之间的单词相关性和单词缺失程度来学习强化奖励,在交叉熵损失和强化奖励的约束下优化CLS模型。为验证所提模型的性能,构建1个有噪声的汉语-越南语CLS语料库。在汉语-越南语和越南语-汉语跨语言摘要数据集上的实验结果表明,所提模型ROUGE分数明显优于其他基线模型,相比NCLS基线模型,该模型ROUGE-1分别提升0.71和0.84,能够有效弱化噪声干扰,从而提高生成摘要的质量。

关键词：汉语-越南语跨语言摘要低资源噪声数据噪声分析多策略强化学习

电解活化次氯酸钠和过硫酸钠对污泥脱水的影响

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环境科学学报 2024年第5期44卷 112-119页

作者：郭绍东尹希黄雨欣王张羽王皓武汉科技大学城市建设学院武汉430065

采用电解零价铁(EZVI)分别活化次氯酸钠(NaClO)和过硫酸盐(PDS)对污泥进行预处理,对比分析了两者对污泥脱水性能及破解效果的影响,并探讨其作用机制.同时,通过毛细吸水时间(CST)评价脱水性能,利用化学需氧量(COD)、氨氮(NH_(4)^(+)-N)... 详细信息

采用电解零价铁(EZVI)分别活化次氯酸钠(NaClO)和过硫酸盐(PDS)对污泥进行预处理,对比分析了两者对污泥脱水性能及破解效果的影响,并探讨其作用机制.同时,通过毛细吸水时间(CST)评价脱水性能,利用化学需氧量(COD)、氨氮(NH_(4)^(+)-N)、溶解性磷(SP)、胞外聚合物(EPS)(包括紧密附着型EPS(TB-EPS)、松散附着型EPS(LB-EPS)、溶解性EPS(S-EPS))考察污泥破解情况.结果表明,最佳处理时间下,EVZINaClO处理污泥,CST下降50.5%,上清液中NH_(4)^(+)-N、COD、SP的增幅分别达到1.8、2.5和10倍;EVZI-PDS组,CST下降37.5%,上清液中NH_(4)^(+)-N和COD的增幅分别为0.51和1.33倍,SP反而有所下降.EVZI-NaClO在改善污泥脱水性能及破解污泥细胞效果上优于EZVI-PDS,且使TB-EPS中荧光物质向S-EPS转化更显著.电子顺磁共振检测结果显示,EZVI-NaClO产生了ClO_(2)·,其强氧化性使污泥EPS破解,蛋白质和多糖结构被破坏,污泥疏水性变强.

关键词：污泥脱水氧化次氯酸纳过硫酸盐零价铁电解

关键节点因果逻辑下的乡村制度变迁研究——基于四川凉山彝族高价彩礼治理案例

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西南石油大学学报（社会科学版） 2024年第2期26卷 19-27页

作者：邝良锋黄禹鑫四川农业大学马克思主义学院四川成都611130

关键节点理论是历史制度主义关于传统制度如何突变的一种新解释。四川凉山彝族高价彩礼治理实践为理解乡村制度变迁提供了一个完整的过程:在乡村秩序维持阶段,容许性条件和生成性条件的双缺位致使传统秩序继续保持路径依赖的历史惯性;... 详细信息

关键节点理论是历史制度主义关于传统制度如何突变的一种新解释。四川凉山彝族高价彩礼治理实践为理解乡村制度变迁提供了一个完整的过程:在乡村秩序维持阶段,容许性条件和生成性条件的双缺位致使传统秩序继续保持路径依赖的历史惯性;在乡村秩序改进阶段,乡村内部萌发生成性条件,但容许性条件的匮乏让传统秩序依然难以实现质变;在乡村秩序突破阶段,外部政策的要求满足变迁的容许性条件,内生力量的需要则满足变迁的生成性条件,二者结合使关键节点的因果逻辑得以有效发挥。在制度变迁过程中,党建引领是关键因素,群众内生力量是基础动因,而自我实施是维持变迁的保障。

关键词：关键节点理论制度变迁条件乡村制度变迁彩礼习俗党建引领

Seabed deposition and erosion change and influence factors in the Yangshan Deepwater Port over the years

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Acta Oceanologica Sinica 2019年第7期38卷 96-106页

作者： Shuhua Zuo Hualiang Xie Xiaoming Ying Cheng Cui yuxin huang Huaiyuan Li Mingxiao Xie National Engineering Laboratory for Port Hydraulic Construction Technology Tianjin Research Institute for Water Transport EngineeringTianjin 300456China Key Laboratory of Engineering Sediment of Ministry of Transport Tianjin Research Institute for Water Transport EngineeringTianjin 300456China South China Sea Institute of Marine Planning and Environmental Research State Oceanic AdministrationGuangzhou 510300China

The seabed scouring and silting are very important to the construction of port and waterway engineering.Seabed deposition and erosion change is complicated due to the influence of sediment supply,human activities and other factors.The Yangshan Deepwater Port is the new deep water harbor,which is an important part of the Shanghai International Shipping Service Center.Its construction has received much attention.At present,the water depth from the 1st to the 3rd harbor district is currently suitable under regular dredging and tidal current action.The fourth harbor district will be built in the world’s largest fully-automated deep water wharf.In the study,bathymetry change of the entire sea area of the Yangshan Deepwater Port and the 4th harbor district(i.e.,PhaseⅣproject)waters were analyzed quantitatively using multiyear bathymetric,hydrological and sediment data.The results show that from 1998 to 2010,seabed changes are characterized by large volumes of erosion and sedimentation,which the southern part was deposited and the northern part was eroded in the inner harbor waters,but the seabed of the Kezhushan inlet was eroded.Seabed changes of PhaseⅣproject waters generally show a scour tendency in recent few years with the annual scour rate about 0.7 m.Among the many factors,the existence of Kezhushan inlet and its influence of the western water flow play an important positive role in water depth changes under the ebb tide action.

关键词： Yangshan Deepwater Port phaseⅣproject water and sediment environment seabed deposition and erosion change diversion dike

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非酒精性脂肪肝与骨质疏松-从临床到分子

中国骨质疏松杂志 2024年第5期30卷 755-760,774页

作者：莫亮黄予心王章正李健雄广州中医药大学广东广州510405 中山市人民医院康复医学科广东中山528400

肝脏和骨骼代谢之间的相互作用对维持机体代谢稳态具有重要作用。非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)和骨质疏松(osteoporosis,OP)是常见的慢性肝脏和骨骼疾病,近年来许多研究提示NAFLD患者中OP发病风险增加,然... 详细信息

肝脏和骨骼代谢之间的相互作用对维持机体代谢稳态具有重要作用。非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)和骨质疏松(osteoporosis,OP)是常见的慢性肝脏和骨骼疾病,近年来许多研究提示NAFLD患者中OP发病风险增加,然而NAFLD合并OP的病理机制尚不明确。本文综述了近年来的相关研究,探讨了NAFLD和OP的临床关系,及NAFLD病理状态下可能对骨代谢造成影响的分子基础,包括炎症因子、骨桥蛋白(OPN)、骨保护素(OPG)、胎球蛋白A(fetuin-A)、胰岛素样生长因子1(IGF-1)、脂联素(Adiponectin)、卵磷脂胆固醇酰基转移酶(LCAT)和维生素D。本研究旨在总结NAFLD合并OP的相关研究进展,以期为相关实验研究及临床防治提供新思路。

关键词：非酒精性脂肪肝骨质疏松骨代谢分子机制