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Dysregulated Hepatic Expression of Glucose Transporter Type-1,Toll-Like Receptor 4,and Nuclear Factor Kappa B in Estrogen-Induced Cholestasis Pregnant Rats with Placental Ischemia-Reperfusion Stress

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Maternal-Fetal Medicine 2022年第1期4卷 17-23页

作者： Fan Zhou Huafang Chen Dan Shan yuxia wu Qian Chen Yayi Hu Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengduSichuan 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University) Ministry of EducationChengduSichuan 610041China

Objective:This study aimed at investigating the expression of nuclear factor kappa B(NF-κB)and mammalian target of rapamycin(mTOR)related signal pathways in liver tissues of intrahepatic cholestasis of pregnancy animal models.Methods:Estrogen(EE)-induced cholestasis and a placental ischemia-reperfusion(IR)model were established in pregnant rats.All pregnant rats were divided into four groups by random number table:EE-IR group(n=6),EE-sham group(n=6),control-IR group(n=6)and control-sham group(n=6).Liver expression of mTOR,its upstream regulator DNA damage response-1(REDD1),and downstream factor glucose transporter type-1(GLUT1),accompanied by NF-κB(p65 is the most important component),its activator toll-like receptor 4(TLR4),and inhibitor IκBα,were detected by western blot analysis and real-time polymerase chain reaction.The intergroup comparisons were performed with a one-way analysis of variance,the comparisons among groups were analyzed with the nonparametric Kruskal-Wallis test.Results:Giving pregnant rats EE alone reduced the hepatic expression of IκBα(0.72±0.20vs.1.01±0.07,P=0.008).Meanwhile,giving pregnant rats placental IR alone increased liver levels of REDD1(3.24±0.98vs.1.06±0.24,P=0.025),GLUT1(2.37±0.82vs.1.09±0.10,P=0.039),TLR4(2.12±0.29vs.1.20±0.28,P=0.010),and p65(2.09±0.85vs.1.04±0.06,P=0.023),and decreased hepatic mTOR(0.50±0.07vs.1.01±0.03,P=0.001)and IκBα(0.61±0.08vs.1.01±0.07,P=0.014)expression.Subjecting EE-treated rats to placental IR did not further alter liver levels of GLUT1(2.02±0.45vs.1.79±0.39,P=0.240),TLR4(2.10±0.74vs.1.60±0.36,P=0.129),or p65(2.41±0.83vs.1.65±0.46,P=0.145),whereas it did decrease hepatic mTOR(0.42±0.09vs.0.90±0.14,P=0.008)and IκBα(0.43±0.09vs.0.72±0.20,P=0.004)expression and enhance REDD1 expression(4.46±0.65vs.2.05±0.47,P=0.009).Placental IR stress did impact the hepatic expression of REDD1-mTOR-GLUT1 and TLR4/NF-κB/IκBαin pregnant rats.Conclusion:Placental IR-induced hepatic GLUT1,TLR4,and p65 alternation,which responded efficiently in control rats,were impaired in

关键词： Cholestasis,intrahepatic Liver mTOR Pregnancy

Research on history of Chinese seismology

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Earthquake Science 2010年第3期23卷 243-257页

作者： Rui Feng yuxia wu Institute of Geophysics China Earthquake Administration Beijing 100081 China China Earthquake Networks Center Beijing 100045 China

The history of Chinese seismology can be traced back to four thousand years before and divided into four stages,i.e.,primitive knowledge,worship of nature,perceptual knowledge and scientific research.The second stage ran in the whole Qin-Han dynasties,and the fourth stage began from Emperor Kangxi in Qing Dynasty and continued to the present.So far China has made four great contributions to seismological development of the world,i.e.,the invention of Heng Zhang＇s seismoscope,great amount of historical records of earthquakes of four thousand years,most abundant anomaly data before earthquakes,and successful practice of earthquake prediction in Haicheng.However,the seismological research in China at present is still on the junior and developing stage.Now we have been carrying on some recessively historical load in our mind such as the subconsciousness of absolute obedience,habit of phenomenological study as well as the methods of philosophical analysis without sincerity.For constructing a high-level Chinese culture in seismological research,we need to pay attention to combining the phenomenological research with experiment,observation and theory study.It is also suggested to take the appropriated measures matched with the present research level in seismology,as well as to promote coexisting and merging of multi-cultures.

关键词： culture tradition phenomenological research earthquake prediction culture in science

Identification of Kalidium species(Chenopodiaceae)by DNA barcoding

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Research in Cold and Arid Regions 2017年第1期9卷 89-96页

作者： XiaoHui Liang yuxia wu State Key Laboratory of Grassland Agro-Ecosystem School of Life SciencesLanzhou UniversityLanzhouGansu 730000China

DNA barcoding is an increasingly prevalent molecular biological technology which uses a short and conserved DNA fragment to facilitate rapid and accurate species identification. Kalidium species are distributed in saline soil habitat throughout Southeast Europe and Northwest Asia, and used mainly as forage grass in China. The discrimination of Ka-lidium species was based only on morphology-based identification systems and limited to recognized species. Here, we tested four DNA candidate loci, one nuclear locus （ITS, internal transcribed spacer） and three plastid loci （rbcL9 matK and ycflb）, to select potential DNA barcodes for identifying different Kalidium species. Results showed that the best DNA barcode was ITS locus, which displayed the highest species discrimination rate （100%）, followed by matK （33.3%）,ycflb （16.7%）, and rbcL （16.7%）. Meanwhile, four loci clearly identified the variant species, Kalidium cuspidatum （Ung.-Stemb.） Gmb.var.A. J. Li,as a single species in Kalidium.

关键词： DNA barcoding Kalidium species identification

医社协同与社区智慧健康小屋:分级诊疗路径再建构

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中国全科医学 2024年第13期27卷 1638-1644页

作者：吴玉霞马洪波米红宁波工程学院人文与艺术学院宁波工程学院“一老一小”发展研究院浙江省宁波市315211 浙江省宁波市之江社会工作服务评估与研究中心宁波工程学院“一老一小”发展研究院 315200

背景分级诊疗自实施以来进展缓慢,其中对多元主体和利益相关者分析的全面性不足是重要原因之一。目的通过分析分级诊疗中多元主体的利益关系,探讨能结合自上而下和自下而上双重路径的机制创新方案,通过政策创新和路径创新,助力多元主体... 详细信息

背景分级诊疗自实施以来进展缓慢,其中对多元主体和利益相关者分析的全面性不足是重要原因之一。目的通过分析分级诊疗中多元主体的利益关系,探讨能结合自上而下和自下而上双重路径的机制创新方案,通过政策创新和路径创新,助力多元主体协同治理,优化分级分流的就医秩序。方法于2022-10-10—2023-03-20选取2个国内先进城市的先进城区(X市S区和N市H区)为典型调研区,通过滚雪球抽样和目的抽样法,选取典型调研区内36位不同利益相关者(涉及市级卫生行政部门、三级医院管理者、三级医院专科医生、社区卫生服务中心管理者、全科医生、健康社工师、患者等7类主体)为研究对象开展深度访谈,运用利益相关者的理论分析方法,分析7大利益主体之间的利益纠葛和掣肘及其对分级诊疗就医秩序健康发展的制约,以探索分级诊疗的机制困境;并对X市S区和N市H区实施健康社工和社区智慧健康小屋试点地区和非试点地区的典型利益相关者的访谈记录进行文本分析,以对比实施前后的效果。结果深度访谈结果显示,“利益相关程度、实施意愿程度、受实施影响程度和对实施影响力”4大维度是影响7类主体实施分级诊疗的主要方面,7大主体有各自利益立场,对分级诊疗起到不同程度的助力和阻力作用,多元主体之间难以形成协同机制是问题的关键。试点地区通过推行社区智慧健康小屋、健康社会工作及其形成的医社协同新机制,有助于增强7大主体的利益相关性,实现更好的分级诊疗秩序。结论社区智慧健康小屋是医社协同新机制的物化空间,健康社工师是新机制的链接点和赋能者。借助于社区智慧健康小屋的新载体和健康社工师的新力量,建构以健康社会工作为中心的医社协同新路径,能实现就医人口的基层前置,对形成分级诊疗的就医秩序起到“增能导流”的作用。

关键词：健康治理分级诊疗医社协同社区智慧健康小屋健康社会工作

Effect of salt stress on the expression of NHX-type Na^+/H^+ antiporters in Populus euphratica and P.pruinosa calli

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Research in Cold and Arid Regions 2014年第1期6卷 66-72页

作者： LiHua Hu yuxia wu State Key Laboratory of Grassland Agro-Ecosystem School of Life SciencesLanzhou University

Populus euphratica and Populuspruinosa, sister species in the Turanga Section （Salicaceae）, growing in semi-arid saline areas are known for their high salinity tolerance. In this study, by combining growth level with Na＋ and K＋ contents, the expression level of vacuolar Na＋/H＋ antiporters was investigated for NaCl-induced changes in P. euphratica and t3. pru- inosa calli. Compared to R euphratica, P. pruinosa calli grew well in 200 mM NaC1 stress from 14. to 21 days. Increasing the stressed time caused an increase in Na＋ content concomitant with a decrease in K＋ content in P. euphratica calli, whereas, with the presence of 200 mM NaCI, K＋ content has a less increase in 14 and 21 days than in 7 days which was detected in R pruinosa calli. The transcript levels of six genes coding for NHX-type Na＋/H＋ antiporters suggest that vacuolar NHX1-NHX6 antiporters play important roles in responding to salt stress in R pruinosa. Our data suggest that there exists a higher salt tolerance for P. pruinosa than P. euphratica at the cellular level, Na＋ avoidance or accumulation is observed in cellular compartments, and that expression of NHX antiporters is linked to the accumulator phenotype.

关键词： P. euphratica 19. pruinosa vacuolar Na＋/H＋ antiporters

Machine Learning(ML)-Assisted Design and Fabrication for Solar Cells

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Energy & Environmental Materials 2019年第4期2卷 280-291页

作者： Fan Li Xiaoqi Peng Zuo Wang Yi Zhou yuxia wu Minlin Jiang Min Xu Institute of Advanced Study Nanchang UniversityNanchang Jiangxi 330031China School of Information Engineering Nanchang UniversityNanchang Jiangxi 330031China Department of Computational Biology Carnegie Mellon UniversityPittsburgh PA 15213USA

Photovoltaic (PV) technologies have attracted great interest due to their capability of generating electricity directly from sunlight. Machine learning(ML) is a technique for computer to learn how to perform a specific task using known data. It can be used in many areas and has become a hot research topic recently due to the rapid accumulation of data and advancement of computer hardware. The application of ML techniques in the design and fabrication of solar cells started slowly but has recently gained tremendous momentum. An exhaustive compilation of the literatures indicates that all the major aspects in the research and development of solar cells can be effectively assisted by ML techniques. If combined with other tools and fed with additional theoretical and experimental data, more accurate and robust results can be achieved from ML techniques. The aspects can be grouped into four categories:prediction of material properties,optimization of device structures, optimization of fabrication processes, and reconstruction of measurement data. A statistical analysis of the literatures shows that artificial neural network (ANN) and genetic algorithm (GA) are the two most applied ML techniques and the topics in the optimization of device structures and optimization of fabrication processes are more popular.This article can be used as a reference by all PV researchers who are interested in ML techniques.

关键词： design and fabrication machine learning optimization solar cell

Differential expression of PeSOS1, PeNhaD1 and PeNHX1 genes between the salt-tolerant Populus euphratica and salt-sensitive P. hopeiensis calli

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Research in Cold and Arid Regions 2010年第1期2卷 43-50页

作者： yuxia wu MOE Key Laboratory of Arid and Grassland Ecology School of Life Sciences Lanzhou University Lanzhou Gansu 730000 China

Populus euphratica Oliv. is of high salinity tolerance and used as a model species for investigating molecular mechanisms of trees＇ responses to salt stress. In the work presented here we found that calli of P. euphratica grew more rapidly and accumulated less Na＋, but more K＋, under salt stress than those of salt-sensitive poplar, Populus hopeiensis. Different types of Na＋/H＋ antiporters （SOS1, NhaD1 and NHX1） were isolated from P. euphratica; all of these genes have been shown to play important roles in plant salt tolerance mechanism in previous studies. Expression profiles of these three genes were compared between P. hopeiensis and P. euphratica in the presence and absence of salt stress by real-time PCR. The three genes were induced in both P. euphratica and P. hopeiensis by salt. Transcript levels of PeNHX1 were lower in P. euphratica than in P. hopeiensis under 150 mM NaCl stress. In addition, transcript levels of PeNhaD1 were lower, while PeSOS1 were higher in P. euphratica than in P. hopeiensis under both stressed and unstressed conditions. The results indicated that P. euphratica up-regulates different genes and consistently maintains both effluxes of Na＋ and high K＋ levels. Our data suggests that differences in gene expression patterns may contribute to the dif-ference in salt tolerance between these two poplars.

关键词： P.euphratica P.hopeiensis Na＋/H＋ antiporters salinity tolerance

Structural and functional alterations in the brains of patients with anisometropic and strabismic amblyopia:a systematic review of magnetic resonance imaging studies

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Neural Regeneration Research 2023年第11期18卷 2348-2356页

作者： yuxia Wang Ye wu Lekai Luo Fei Li Huaxi MR Research Center(HMRRC) Department of RadiologyWest China Hospital of Sichuan UniversityChengduSichuan ProvinceChina Department of Ophthalmology Laboratory of Optometry and Vision SciencesWest China Hospital/West China School of MedicineSichuan UniversityChengduSichuan ProvinceChina Department of Radiology West China Second Hospital of Sichuan UniversityChengduSichuan ProvinceChina

Amblyopia is the most common cause of vision loss in children and can persist into adulthood in the absence of effective intervention.Previous clinical and neuroimaging studies have suggested that the neural mechanisms underlying strabismic amblyopia and anisometropic amblyopia may be different.Therefore,we performed a systematic review of magnetic resonance imaging studies investigating brain alterations in patients with these two subtypes of amblyopia;this study is registered with PROSPERO(registration ID:CRD42022349191).We searched three online databases(PubMed,EMBASE,and Web of Science) from inception to April 1,2022;39 studies with 633 patients(324patients with anisometropic amblyo pia and 309 patients with strabismic amblyopia) and 580 healthy controls met the inclusion criteria(e.g.,case-control designed,pee r-reviewed articles) and were included in this review.These studies highlighted that both strabismic amblyopia and anisometropic amblyopia patients showed reduced activation and distorted topological cortical activated maps in the striate and extrastriate co rtices during tas k-based functional magnetic resonance imaging with spatial-frequency stimulus and retinotopic representations,respectively;these may have arisen from abnormal visual experiences.Compensations for amblyopia that are reflected in enhanced spontaneous brain function have been reported in the early visual cortices in the resting state,as well as reduced functional connectivity in the dorsal pathway and structural connections in the ventral pathway in both anisometro pic amblyopia and strabismic amblyopia patients.The shared dysfunction of anisometro pic amblyopia and strabismic amblyopia patients,relative to controls,is also chara cterized by reduced spontaneous brain activity in the oculomotor co rtex,mainly involving the frontal and parietal eye fields and the cerebellu m;this may underlie the neural mechanisms of fixation instability and anomalous saccades in amblyopia.With regards to specific alterations of the two forms of amblyo

关键词： amblyopia anisometropia brain function magnetic resonance imaging oculomotor system precortical pathway strabismus structure visual cortex

Expression analysis of a Na^+/H^+ antiporter gene PeNHX1 from Populus euphratica

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Research in Cold and Arid Regions 2009年第6期1卷 541-548页

作者： yuxia wu JianQuan Liu MOE Key Laboratory of Arid and Grassland Ecology School of Life Sciences Lanzhou University Lanzhou 730000 China

Na+/H+ antiporters play an important role in the salt tolerance of a wide variety of plants. Using the rapid amplification of cDNA ends method, a Na+/H+ antiporter gene (PeNHX1) was isolated from Populus euphratica. The deduced amino acid sequence contained 528 amino acid residues with a conserved amiloride-binding domain ((LFFIYLLPPI86)-L-77) and shared more than 68% identity with that of AtNHX1 from Arabidopsis thaliana. PeNHX1 can confer resistance to Na+, as well as Li+, to (EP432) an Escherichia coli strain deficient in both nhaA and nhaB, thus proving that it is a functional Na+/H+ antiporter. PeNHX1 expression profile in EP432 reflected pH independent manner. PeNHX1 expression was regulated by salt at the transcriptional level. Meanwhile, results demonstrated that transcripts of PeNHX1 in P. euphratica calli showed a salt dependent response, and thus provide a valuable tool for studying signaling and biochemical pathways involved in salt recognition and response in P. euphratica.

关键词： Na+/H+ antiporter gene,salt tolerance,P.euphratica