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Analyzing the cellular and molecular atlas of ovarian mesenchymal cells provides a strategy against female reproductive aging

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Science China(Life Sciences) 2023年第12期66卷 2818-2836页

作者： Longzhong Jia Wenji Wang Jing Liang Shudong Niu Yibo Wang Jian Yang Lingyu Li Ge Wang Xueqiang Xu Lu Mu Kaixin Cheng Xuebing Yang Yijing Wang Haoshu Luo Guoliang Xia yuwen ke Yan Zhang Hua Zhang State key Laboratory of Animal Biotech Breeding College of Biological SciencesChina Agricultural UniversityBeijing 100193China School of Life Sciences Taizhou UniversityTaizhou 318000China National Institute of Biological Sciences Beijing 102206China key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China College of Life ScienceNingxia UniversityYinchuan 750021China

Ovarian mesenchymal cells(oMCs)constitute a distinct microenvironment that supports folliculogenesis under physiological *** of exogenous non-ovarian mesenchymal-related cells has been reported to be an efficient approach to improve ovarian ***,the development and cellular and molecular characteristics of endogenous oMCs remain largely *** this study,we surveyed the single-cell transcriptomic landscape to dissect the cellular and molecular changes associated with the aging of oMCs in *** results showed that the oMCs were composed of five ovarian differentiatedMC(odMC)populations and one ovarian mesenchymal progenitor(oMP)cell *** cells could differentiate into various odMCs via an oMP-derived route to construct the ovarian stroma *** analysis revealed that ovarian aging was associated with decreased quantity of oMP cells and reduced quality of *** on the findings of bioinformatics analysis,we designed different strategies involving supplementation with young oMCs to examine their effects on female fertility and *** functional investigations revealed that oMCs supplementation prior to ovarian senescence was the optimal method to improve female fertility and extend the reproductive lifespan of aged females in the longterm.

关键词： single-cell analysis ovary mesenchyma mesenchymal progenitor cells aging infertility

动物早期胚胎发育中染色质结构的继承和重编程

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遗传 2018年第11期40卷 977-987页

作者：柯玉文刘江中国科学院北京基因组研究所中国科学院基因组科学与信息重点实验室北京100101 中国科学院大学北京100049

染色质开放性和染色质三维高级结构在基因表达和调控中发挥着非常重要的作用,广泛参与分化、发育、肿瘤发生等细胞生理过程,是表观遗传研究的热点领域之一。动物胚胎发育起始于终端分化的卵子受精形成全能性的受精卵。在精卵结合的过程... 详细信息

染色质开放性和染色质三维高级结构在基因表达和调控中发挥着非常重要的作用,广泛参与分化、发育、肿瘤发生等细胞生理过程,是表观遗传研究的热点领域之一。动物胚胎发育起始于终端分化的卵子受精形成全能性的受精卵。在精卵结合的过程中,染色质开放性和染色质三维高级结构发生了剧烈的变化,经历继承、重编程、重新建立的过程,并指导调控受精卵分化发育最终成为多细胞、多器官组织的新生命个体。本文介绍了近年来研究染色质开放性和染色质三维高级结构的实验分析技术手段,染色质结构在动物早期胚胎发育过程中的变化规律及其在早期胚胎发育中的作用,染色质结构与其他表观遗传信息(甲基化、组蛋白修饰等)关系方面的重要研究进展和存在的科学问题,以期为表观遗传调控早期胚胎发育的研究提供参考。

关键词：表观遗传胚胎发育染色质开放性染色质三维高级结构

Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology

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Journal of Genetics and Genomics 2017年第10期44卷 475-481页

作者： Guoqiang Li Yang Yu Yong Fan Congru Li Xiaocui Xu Jialei Duan Rong Li Xiangjin Kang Xin Ma Xuepeng Chen yuwen ke Jie Yan Ying Lian Ping Liu Yue Zhao Hongcui Zhao Yaoyong Chen Xiaofang Sun Jianqiao Liu Jie Qiao Jiang Liu Ministry of Education key Laboratory of Assisted Reproduction Beijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductiveTechnology Center of Reproductive Medicine Peking University Third Hospital Beijing 100191 China GAS key Laboratory of Genome Sciences and Information Collaborative Innovation Center of Genetics and Development Beijing Institute of GenomicsGAS Beijing 160101 China key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150China University of Chinese Academy of Sciences Beijing 100029 China

Proper reprogramming of parental DNA methylomes is essential for mammalian embryonic development. However, it is unknown whether abnormal methylome reprogramming occurs and is associated with the failure of embryonic development. Here we analyzed the DNA methylomes of 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology （ART） practices. Our data reveal that the global methylation levels of high-quality blastocysts are similar （0.30 ± 0.02, mean ± SD）, while the methylation levels of low-quality blastocysts are divergent and away from those of high-quality blastocysts. The proportion of blastocysts with a methylation level falling within the range of 0.30± 0.02 in different grades correlates with the live birth rate for that grade. Moreover, abnormal methylated regions are associated with the failure of embryonic development. Furthermore, we can use the methylation data of cells biopsied from trophectoderm to predict the blastocyst methylation level as well as to detect the aneuploidy of the blastocysts. Our data indicate that global abnormal methylome reprogramming often occurs in human embryos, and suggest that DNA methylome is a potential biomarker in blastocyst selection in ART.

关键词： Human Abnormal Blastocyst Methylome ART

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表观遗传信息在动物中的跨代遗传和重编程

中国科学：生命科学 2021年第5期51卷 556-566页

作者：荐思婧宁超高磊柯玉文刘江中国科学院北京基因组研究所基因组科学与信息重点实验室北京100101 中国科学院大学北京100049

在动物早期发育过程中,无论是生物个体的形态还是基因的表达都发生了巨大的变化,而表观遗传信息在此过程中起着重要的作用.近年来,随着基因组等技术的发展,人们对不同表观遗传信息在配子形成、跨代遗传和早期发育过程中的认识越来越丰富... 详细信息

在动物早期发育过程中,无论是生物个体的形态还是基因的表达都发生了巨大的变化,而表观遗传信息在此过程中起着重要的作用.近年来,随着基因组等技术的发展,人们对不同表观遗传信息在配子形成、跨代遗传和早期发育过程中的认识越来越丰富,发现在这些过程中往往会出现全基因组层次的重编程.本文主要介绍了DNA甲基化、染色质开放性、组蛋白修饰及染色体三维结构这些表观遗传信息在跨代遗传和胚胎发育时期的重编程的进展,以及不同物种间的保守性和进化性.

关键词：表观遗传 DNA甲基化染色质开放性组蛋白修饰染色体三维结构配子形成早期胚胎发育

两种不同方法判断左侧双腔支气管插管位置的比较

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外科研究与新技术 2019年第3期8卷 177-179页

作者：帅兵柯毓文朱彩艳赵昭刘志恒深圳市第二人民医院麻醉科

目的探讨支气管套囊测压法及纤维支气管镜法判断左侧双腔支气管插管定位的准确性。方法选择100例需左侧双腔支气管插管单肺通气行胸科手术的患者,随机分为两组,每组50例。双腔支气管插管后分别采用支气管套囊测压法及纤维支气管镜法判... 详细信息

目的探讨支气管套囊测压法及纤维支气管镜法判断左侧双腔支气管插管定位的准确性。方法选择100例需左侧双腔支气管插管单肺通气行胸科手术的患者,随机分为两组,每组50例。双腔支气管插管后分别采用支气管套囊测压法及纤维支气管镜法判断双腔支气管的位置。结果支气管套囊测压法、纤维支气管镜法定位准确率分别为88%、100%,纤维支气管镜法明显高于支气管套囊测压法(P0.05)。结论支气管套囊测压法简单实用,具较高的定位准确率和较低的并发症发生率。

关键词：纤维支气管镜双腔支气管定位

从海马区自噬水平探讨右美托咪定缓解老年患者全身麻醉术后认知功能障碍的作用机制

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中国临床药学杂志 2020年第2期29卷 99-102页

作者：帅兵柯毓文朱彩艳赵昭刘志恒广东省深圳市第二人民医院麻醉科深圳518035

目的从海马区自噬水平探讨右美托咪定缓解老年患者全身麻醉术后认知功能障碍的作用机制。方法选取2015年1月至2018年12月行全身麻醉手术治疗的80例老年患者,随机分为观察组和对照组,各40例。对照组给予常规的全身静脉麻醉,观察组在手术... 详细信息

目的从海马区自噬水平探讨右美托咪定缓解老年患者全身麻醉术后认知功能障碍的作用机制。方法选取2015年1月至2018年12月行全身麻醉手术治疗的80例老年患者,随机分为观察组和对照组,各40例。对照组给予常规的全身静脉麻醉,观察组在手术结束前30 min采用静脉泵给予右美托咪定。比较术后2组患者的镇痛效果、认知功能评分、海马区自噬水平和不良反应发生率等。结果观察组患者在术后6、12、24 h的视觉模拟评分法(VAS)评分均低于对照组(P0.05)。结论右美托咪定在老年患者全身麻醉术后镇痛效果良好,能够改善患者术后的认知功能,上调海马区自噬标记蛋白水平,且用药安全性好,其改善患者认知功能可能与抑制海马区自噬有关。

关键词：全身静脉麻醉右美托咪定老年患者海马区自噬水平药物作用机制

Load and velocity boundaries of oil-based superlubricity using 1,3-diketone

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Friction 2023年第5期11卷 704-715页

作者： Yuyang YUAN Tobias AMANN yuwen XU Yan ZHANG Jingfu CHEN Chenqing YUAN ke LI School of Transportation and Logistics Engineering Wuhan University of TechnologyWuhan 430063China Reliability Engineering Institute National Engineering Research Center for Water Transport SafetyMOSTWuhan 430063China Fraunhofer Institute for Mechanics of Materials IWM Freiburg 79108Germany

The clarification of the critical operating conditions and the failure mechanism of superlubricity systems is of great significance for seeking appropriate applications in *** this work,the superlubricity region of 1,3-diketone oil EPND(1-(4-ethyl phenyl)nonane-1,3-dione)on steel surfaces was identified by performing a series of ball-on-disk rotation friction tests under various normal loads(3.5–64 N)and sliding velocities(100–600 mm/s).The result shows that beyond certain loads or velocities superlubricity failed to be reached due to the following negative effects:(1)Under low load(≤3.5 N),insufficient running-in could not ensure good asperity level conformity between the upper and lower surfaces;(2)the high load(≥64 N)produced excessive wear and big debris;(3)at low velocity(≤100 mm/s),the weak hydrodynamic effect and the generated debris deteriorated the lubrication performance;(4)at high velocity(≥500 mm/s),oil migration occurred and resulted in oil *** order to expand the load and velocity boundaries of the superlubricity region,an optimized running-in method was proposed to avoid the above negative *** initially operating a running-in process under a suitable combination of load and velocity(e.g.16 N and 300 mm/s)and then switching to the target certain higher or lower load/velocity(e.g.100 N),the superlubricity region could break through its original *** result of this work suggests that oil-based superlubricity of 1,3-diketone is a promising solution to friction reduction under suitable operating conditions especially using a well-designed running-in strategy.

关键词： macroscopic superlubricity 1,3-diketone oil running-in process load and velocity boundaries

Genome-wide analyses on transcription factors and their potential microRNA regulators involved in maize male fertility

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The Crop Journal 2021年第6期9卷 1248-1262页

作者： Ziwen Li Taotao Zhu Shuangshuang Liu Yilin Jiang Haoyun Liu yuwen Zhang ke Xie Jinping Li Xueli An Xiangyuan Wan Zhongzhi International Institute of Agricultural Biosciences Biology and Agriculture Research CenterUniversity of Science and Technology BeijingBeijing 100024China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing 100192China

Anther development is a programmed biological process crucial to plant male reproduction. Genomewide analyses on the functions of transcriptional factor(TF) genes and their microRNA(miRNA) regulators contributing to anther development have not been comprehensively performed in maize. Here, using published RNA-Seq and small RNA-Seq(sRNA-Seq) data from maize anthers at ten developmental stages in three genic male-sterility(GMS) mutants(ocl4, mac1, and ms23) and wild type W23, as well as newly sequenced maize anther transcriptomes of ms7-6007 and lob30 GMS mutants and their WT lines, we analyzed and found 1079 stage-differentially expressed(stage-DE) TF genes that can be grouped into six(premeiotic, meiotic, postmeiotic, premeiotic-meiotic, premeiotic-postmeiotic, and meiotic-postmeiotic clusters) expression clusters. Functional enrichment combined with cytological and physiological analyses revealed specific functions of genes in each expression cluster. In addition, 118 stage-DE miRNAs and99 miRNA-TF gene pairs were identified in maize anthers. Further analyses revealed the regulatory roles of zma-miR319 and zma-miR159 as well as ZmMs7 and ZmLOB30 on ZmGAMYB expression. Moreover,ZmGAMYB and its paralog ZmGAMYB-2 were demonstrated as novel maize GMS genes by CRISPR/Cas9 knockout analysis. These results extend our understanding on the functions of miRNA-TF gene regulatory pairs and GMS TF genes contributing to male fertility in plants.

关键词： Transcription factor gene microRNA Anther development Male fertility Genic male sterility

Influence of MoSi_(2) on oxidation protective ability of TaB_(2)-SiC coating in oxygen-containing environments within a broad temperature range

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Journal of Advanced Ceramics 2020年第6期9卷 703-715页

作者： Xuanru REN Junshuai LV Wei LI yuwen HU ke SUN Can MA Hong’ao CHU Weiguang WANG Leihua XU Ziyu LI Peizhong FENG School of Materials Science and Engineering China University of Mining and TechnologyXuzhou 221116China

TaB_(2)-SiC coating modified by different content of MoSi_(2) was fabricated on graphite substrate with SiC inner coating by liquid phase sintering to elevate the anti-oxidation capability of the TaB_(2)-SiC *** compared to the sample with the TaB_(2)-40wt% SiC coating,the coating sample modified with MoSi_(2) exhibited a weight gain trend at lower temperatures,the fastest weight loss rate went down by 76%,and the relative oxygen permeability value reduced from about 1% to near *** importantly,the large amount of SiO_(2) glass phase produced over the coating during oxidation was in contact with the modification of MoSi_(2),which was proved to be beneficial to the dispersion of Ta-oxides.A concomitantly formed continuous Ta-Si-O-B compound glass layer showed excellent capacity to prevent oxygen ***,when the TaB_(2) content was sacrificed to increase the MoSi_(2) content,the relative oxygen permeability of the coating increased instead of ***,on the basis of ample TaB_(2) content,increasing the MoSi_(2) content of the coating is conducive to reducing the relative oxygen permeability of the coatings in a broad temperature region.

关键词： MoSi_(2) TaB_(2)-SiC coating liquid phase sintering compound glass layer relative oxygen permeability