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Size-dependent gene delivery of amine-modified silica nanoparticles

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Nano Research 2016年第2期9卷 291-305页

作者： Meihua Yu yuting niu Jun Zhang Hongwei Zhang Yannan Yang Elena Taran Siddharth Jambhrunkar Wenyi Gu Peter Thorn Chengzhong Yu Australian Institute for Bioengineering and Nanotechnology The University of Queensland Brisbane QLD 4072 Australia Australian National Fabrication Facility-QLD Node Brisbane QLD 4072 Australia School of Biomedical Sciences The University of Queensland Brisbane QLD 4072 Australia

Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse St6ber spheres （NH2-SS） with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein （GFP）（pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp） were studied in HEK293T cells. NH2-SS with a diameter of 330 nm （NH2-SS330） showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and cellular uptake performance of NH2-SS330 and pcDNA conjugates. NH2-SS330 also demonstrated the highest transfection efficiency for plasmid DNA （pDNA） with a bigger size of 8.9 kbp. To our knowledge, this study is the first to demonstrate the significance of particle size for gene transfection efficiency in silica-based gene delivery systems. Our findings are crucial to the rational design of synthetic vectors for gene therapy.

关键词： silica nanoparticles gene delivery plasmid DNA particle sizes cellular uptake

A general and simple method for evaluating the electrical transport performance of graphene by the van der Pauw–Hall measurement

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Science Bulletin 2018年第22期63卷 1521-1526页

作者： Fangzhu Qing Yang Shu Linsen Qing yuting niu He Guo Shuyi Zhang Chunlin Liu Changqing Shen Wanli Zhang Samuel S.Mao Wenjuan Zhu Xuesong Li State Key Laboratory of Electronic Thin Films and Integrated Devices &School of Electronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 610054China Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041China National Engineering Research Center of Electromagnetic Radiation Control Materials University of Electronic Science and Technology of ChinaChengdu 610054China Institute of New Energy Shenzhen 518031China Department of Mechanical Engineering University of California at BerkeleyBerkeleyCA 94720USA Department of Electrical and Computer Engineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Expected for many promising applications in the field of electronics and optoelectronics, a reliable method for the characterization of graphene electrical transport properties is desired to predict its device performance or provide feedback for its ***, the commonly used methods of extracting carrier mobility from graphene field effect transistor or Hall-bar is time consuming, expensive, and significantly affected by the device fabrication process other than graphene *** we reported a general and simple method to evaluate the electrical transport performance of graphene by the van der Pauw–Hall *** annealing graphene in vacuum to remove the adsorbed dopants and then exposing it in ambient surroundings, carrier mobility as a function of density can be measured with the increase of carrier density due to the dopant re-adsorption from the ***, the relationship between the carrier mobility and density can be simply fitted with a power equation to the first level approximation, with which any pair of measured carrier mobility and density can be normalized to an arbitrary carrier density for *** experimentally demonstrated the reliability of the method, which is much simpler than making devices and may promote the standard making for graphene characterization.

关键词： Graphene Van der Pauw Hall Chemical vapor deposition (CVD)

Alkaline activation of endogenous latent TGFβ1 by an injectable hydrogel directs cell homing for in situ complex tissue regeneration

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Bioactive Materials 2022年第9期7卷 316-329页

作者： Sainan Wang yuting niu Peipei Jia Zheting Liao Weimin Guo Rodrigo Cotrim Chaves Khanh-Hoa Tran-Ba Ling He Hanying Bai Sam Sia Laura J.Kaufman Xiaoyan Wang Yongsheng Zhou Yanmei Dong Jeremy J.Mao Department of Cariology and Endodontology Peking University School and Hospital of StomatologyBeijing100081China Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing100081China Center for Craniofacial Regeneration Columbia UniversityNew YorkNY10032USA Department of Biomedical Engineering Columbia UniversityNew YorkNY10027USA Department of Chemistry Columbia UniversityNew YorkNY10027USA

Utilization of the body’s regenerative potential for tissue repair is known as in situ tissue ***,the use of exogenous growth factors requires delicate control of the dose and delivery strategies and may be accompanied by safety,efficacy and cost *** this study,we developed,for the first time,a biomaterial-based strategy to activate endogenous transforming growth factor beta 1(TGFβ1)under alkaline conditions for effective in situ tissue *** demonstrated that alkaline-activated TGFβ1 from blood serum,bone marrow fluids and soaking solutions of meniscus and tooth dentin was capable of increasing cell recruitment and early differentiation,implying its broad ***,we engineered an injectable hydrogel(MS-Gel)consisting of gelatin microspheres for loading strong alkaline substances and a modified gelatin matrix for hydrogel click *** vitro models showed that alkaline MS-Gel controllably and sustainably activated endogenous TGFβ1 from tooth dentin for robust bone marrow stem cell *** importantly,infusion of in vivo porcine prepared root canals with alkaline MS-Gel promoted significant pulp-dentin regeneration with neurovascular stroma and mineralized tissue by endogenous proliferative ***,this work offers a new bench-to-beside translation strategy using biomaterial-activated endogenous biomolecules to achieve in situ tissue regeneration without the need for cell or protein delivery.

关键词： Endogenous TGFβ1 Injectable alkaline hydrogel Cell homing Pulp-dentin complex In situ tissue regeneration

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淮南矿区土壤重金属污染状况评价及管理对策

中国资源综合利用 2017年第6期35卷 129-130页

作者：牛玉亭淮南矿业集团资源环境管理部安徽淮南232001

本文以淮南矿业集团所辖3 000 km^2矿区土壤为研究对象,共采集土壤表层样品269件,通过对样品中重金属元素测定和数据分析,采用单因子指数法、内梅罗综合指数法和多指标生态评价法对土壤中As、Cu、Zn、Cr、Ni、Cd、Pb、Hg8种重金属污染... 详细信息

本文以淮南矿业集团所辖3 000 km^2矿区土壤为研究对象,共采集土壤表层样品269件,通过对样品中重金属元素测定和数据分析,采用单因子指数法、内梅罗综合指数法和多指标生态评价法对土壤中As、Cu、Zn、Cr、Ni、Cd、Pb、Hg8种重金属污染现状进行检测、评价,提出煤矿区土壤污染环境管理对策措施。

关键词：土壤污染重金属评价环境管理

汇率升值与通货膨胀对我国股市影响的机理研究

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科技创业月刊 2008年第10期21卷 36-37页

作者：牛玉婷对外经济贸易大学国际经济贸易学院北京100029

自2005年中国实施汇率改革以来,就开始面临着汇率加速升值和通货膨胀率稳步上涨的局面。从当前这种经济宏观层面背景入手,采取理论解释结合实证来研究我国外汇储备、人民币汇率、通货膨胀和利率之间的联动关系,探讨这种关系对我国股市... 详细信息

自2005年中国实施汇率改革以来,就开始面临着汇率加速升值和通货膨胀率稳步上涨的局面。从当前这种经济宏观层面背景入手,采取理论解释结合实证来研究我国外汇储备、人民币汇率、通货膨胀和利率之间的联动关系,探讨这种关系对我国股市的影响机理,并给出总的投资原则。

关键词：汇率通货膨胀利率股市影响机理

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淮南矿区煤矸石资源化利用实践

中国资源综合利用 2017年第8期35卷 41-42,46页

作者：牛玉亭淮南矿业集团资源环境管理部安徽淮南232001

淮南矿区煤炭储量丰富,淮南矿业集团作为国家首批循环经济试点企业,近几年在矸石综合利用方面进行了积极探索和实践,从"堆存为主"转为"利用为主",在不断利用存量矸石的同时,新建矿井不设永久矸石山(堆场),产煤矸石综合利用率达到100%以... 详细信息

淮南矿区煤炭储量丰富,淮南矿业集团作为国家首批循环经济试点企业,近几年在矸石综合利用方面进行了积极探索和实践,从"堆存为主"转为"利用为主",在不断利用存量矸石的同时,新建矿井不设永久矸石山(堆场),产煤矸石综合利用率达到100%以上,煤矸石存量不断减少。本文对淮南矿区的煤矸石资源化利用实践进行介绍,分析了煤矸石综合利用存在的问题,提出了煤矸石资源化管理与利用的建议。

关键词：煤矸石资源化利用实践

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国内口腔专业护理教育发展与前景

护理学杂志 2019年第14期34卷 1-4页

作者：李秀娥王春丽牛玉婷北京大学口腔医院护理部

介绍我国口腔专业护理教育现状,对口腔护理教育途径、教育内容、教学模式三方面进行论述,并对口腔专业护理教育的前景提出展望,提出应推进口腔专科教学体系的建立,不断拓展职业范围,促进我国口腔护理人才培养。

关键词：口腔护理专业护理教育专科护士口腔专科护士课程设置

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淮南迪沟采煤沉陷区水体水质特征与评价

水资源与水工程学报 2021年第2期32卷 58-65页

作者：陈从磊谢毫陈业禹牛玉亭范佳民淮南矿业(集团)有限责任公司煤业分公司资源环保部安徽淮南232001 煤矿生态环境保护国家工程实验室安徽淮南232001 安徽大学资源与环境工程学院安徽省矿山生态修复工程实验室安徽合肥230601

为了解淮南迪沟采煤沉陷区水体水质状况,将该沉陷积水区水域划分为3个单元(济河、沉陷积水区、对照区),采集不同单元样品,测试分析其水质特征,并利用单因子和综合水质标识指数法进行评价。结果表明:济河和沉陷积水区均为弱碱性水体,水... 详细信息

为了解淮南迪沟采煤沉陷区水体水质状况,将该沉陷积水区水域划分为3个单元(济河、沉陷积水区、对照区),采集不同单元样品,测试分析其水质特征,并利用单因子和综合水质标识指数法进行评价。结果表明:济河和沉陷积水区均为弱碱性水体,水质比较澄清,透明度好。氮磷营养盐指标总体含量表现为济河最高,这与济河周边的农业面源污染有关;重金属Cu、Zn、Pb和As在沉陷积水区均出现一定程度的富集,与沉陷区周边充填煤矸石中重金属淋溶释放、汽车尾气的排放以及地表扬尘有关;通过对沉陷积水区距离济河由近及远每隔15 m采集水样的纵向采样点C1、C2、C3分析得出,其TN和NH4^(+)分布表现出距离济河越近,则含量越低,而TP、Cu、Zn、Pb和As表现出相反的规律性;单因子水质标识指数结果显示,Hg和CODMn在济河和沉陷积水区污染相对严重;综合水质标识指数法显示,3个单元综合水质指标Pi均符合Ⅲ级标准。在交汇水域,济河采样点J3、J4与沉陷积水区采样点C1、C2的水质状况表现为J3(2.320)

关键词：采煤沉陷积水区水质特征水质评价综合水质标识指数淮南迪沟镇

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三医联动视角下老年慢病管理发展现状及对策

卫生经济研究 2023年第4期40卷 48-51,55页

作者：牛雨婷赵允伍王晓松王珩安徽医科大学卫生管理学院安徽合肥230032 安徽医科大学第一附属医院安徽合肥230022

当前,我国老年慢病管理已取得阶段性成效,但仍存在老年慢病优质资源失衡、基层医务人员积极性不高、医保医药局限及信息化规范程度较低等问题。对此,从三医联动视角,提出老年慢病管理的对策:医疗多方协同,坚实优质老年慢病服务基础;医... 详细信息

当前,我国老年慢病管理已取得阶段性成效,但仍存在老年慢病优质资源失衡、基层医务人员积极性不高、医保医药局限及信息化规范程度较低等问题。对此,从三医联动视角,提出老年慢病管理的对策:医疗多方协同,坚实优质老年慢病服务基础;医保延伸保障范围,探索医保规范化管理;医药扩大目录,畅通老年慢病药物供应链;普及数字化应用,提高老年慢病管理信息化水平。

关键词：老年慢病管理健康老龄化三医联动医疗联合体