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CRISPR/Cas9-mediated NlInR2 mutants:Analyses of residual mRNA and truncated proteins

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Journal of Integrative Agriculture 2024年第6期23卷 2006-2017页

作者： Jun Lü Jingxiang Chen yutao hu Lin Chen Shihui Li Yibing Zhang Wenqing Zhang State Key Laboratory of Biocontrol/School of Life Sciences Sun Yat-sen UniversityGuangzhou 510275China Development of Food Science and Engineering Moutai InstituteRenhuai 564507China

CRISPR/Cas9 technology is a powerful genome manipulation tool in ***,little is known about whether mRNA and protein of a target gene are completely cleared in homozygous *** study generated homozygous mutants of the insulin receptor gene 2(NlInR2)in the brown planthopper(Nilaparvata lugens)using CRISPR/Cas9 genome *** frameshift mutants,E5_D17 and E6_I7,differentiated towards long wings,but there were differences in wing morphology,with E5_D17 showing wing *** investigations revealed the presence of residual expression of NlInR2 mRNA in both mutants,as well as the occurrence of spliceosomes featuring exon skipping splicing in E5_***,the E5_D17 exhibited the detection of N-terminally truncated NlInR2 *** interference experiments indicated that the knockdown of NlInR2 expression in the E5_D17 mutant line increased the proportion of wing deformities from 11.1 to 65.6%,suggesting that the residual NlInR2 mRNA of the E5_D17 mutant might have retained some genetic *** results imply that systematic characterization of residual protein expression or function in CRISPR/Cas9-generated mutant lines is necessary for phenotypic interpretation.

关键词： CRISPR/Cas9 Nilaparvata lugens residual mRNA skipping exon truncated protein

Enhanced Q-switching performance of magnetite nanoparticle via compositional engineering with Ti_(3)C_(2) MXene in the near infrared region

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Journal of Materials Science & Technology 2021年第22期81卷 51-57页

作者： yutao hu Hongwei Chu Daozhi Li Ying Li Shengzhi Zhao Li Dong Dechun Li School of Information Science and Engineering Shandong UniversityQingdao266237China Key Laboratory of Colloid and Interface Chemistry Ministry of Educationand School of Chemistry and Chemical EngineeringShandong UniversityJinan250100China

In this work,we reported a new strategy to improve the nonlinear saturable absorption performance of magnetite(Fe_(3)O_(4))nanoparticles(FONPs)via the compositional engineering with the Ti_(3)C_(2) MXene in the near-infrared(NIR)*** on the DFT simulation,the band structures and work function were significantly modified by the Ti_(3)C_(2) MXene *** using the open-aperture Z-scan technology,the nonlinear optical features of the FONPs@Ti_(3)C_(2) nanocomposite were significantly improved,showing the great potential as the saturable absorber in the pulsed *** the nanocomposite as the saturable absorber,the passively Q-switched Nd:GdVO4 lasers emitted much shorter pulse durations when compared with the pristine FONP saturable *** findings indicated that FONPs@Ti_(3)C_(2) heterostructure was a promising saturable absorber for the short pulse generation in the NIR region.

关键词： Fe_(3)O_(4)nanoparticle Ti_(3)C_(2)Mxene Heterostructure Saturable absorber Q-switching

爆炸作用下浅埋直墙拱结构的毁伤评估方法与毁伤判据选择

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现代应用物理 2024年第3期15卷 139-147页

作者：柏准胡玉涛钱秉文李献陈昊张宁西北核技术研究所西安710024 陆军工程大学爆炸冲击防灾减灾国家重点实验室南京210007 南京理工大学理学院南京210094

为提出1种针对爆炸作用下浅埋拱结构毁伤的可靠评估方法,对结构毁伤评估的方法和毁伤判据的选择展开了讨论。通过分析钢筋混凝土直墙拱的土中浅埋爆炸试验以及有限元计算结果,将直墙拱结构爆炸作用下的破坏模式总结为弯曲破坏、弯曲-剪... 详细信息

为提出1种针对爆炸作用下浅埋拱结构毁伤的可靠评估方法,对结构毁伤评估的方法和毁伤判据的选择展开了讨论。通过分析钢筋混凝土直墙拱的土中浅埋爆炸试验以及有限元计算结果,将直墙拱结构爆炸作用下的破坏模式总结为弯曲破坏、弯曲-剪切破坏、局部震塌破坏、局部冲切破坏四种破坏模式。总结了结构目标物理毁伤评估的一般思路,讨论了不同破坏模式下毁伤判据的选择,为结构的毁伤等级评估工作的开展奠定了基础。归纳了毁伤判据与毁伤形态、毁伤等级的映射关系,结合毁伤形态对数值模拟结果划分毁伤等级,并比较了不同毁伤判据对等级评估结果准确性的影响,可为毁伤评估判据的选取提供了参考。

关键词：浅埋爆炸直墙拱物理毁伤评估毁伤判据

环泊酚对体外循环下二尖瓣置换术麻醉诱导期的血流动力学影响

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重庆医学 2024年第9期53卷 1339-1343页

作者：宋波张永洪李军彭健胡玉涛绵阳市麻醉科与神经调控重点实验室/绵阳市中心医院麻醉科四川绵阳621000

目的通过血流动力学监测明确环泊酚、丙泊酚对二尖瓣置换术患者诱导期血流动力学及心脏做功的影响。方法选取该院2022年6月至2023年6月拟接受体外循环下二尖瓣置换术患者90例,随机分成2组,环泊酚组(P组,n=45)、丙泊酚组(B组,n=45)。观... 详细信息

目的通过血流动力学监测明确环泊酚、丙泊酚对二尖瓣置换术患者诱导期血流动力学及心脏做功的影响。方法选取该院2022年6月至2023年6月拟接受体外循环下二尖瓣置换术患者90例,随机分成2组,环泊酚组(P组,n=45)、丙泊酚组(B组,n=45)。观察患者给药前(T_(0))和给药后5 min(T_(1))的心率(HR)、血氧饱和度(SpO_(2))、脑频双电指数镇静指数(ICO1)、平均动脉压(MAP)、中心静脉压(CVP)、每搏量(SV)、心输出量(CO)、外周血管阻力(SVR)、心脏循环效率(CCE)、每搏变异度(SVV)、反映心肌收缩力的最大压力梯度(dp/dt)。结果P组T_(1)时ICO1、MAP、SV、CO和SVR较T_(0)时下降,CCE较T_(0)时升高,差异有统计学意义(P0.05);B、P两组T_(1)时HR、SpO_(2)、ICO1、CVP、SVV比较差异也无统计学意义(P>0.05);P组T_(1)时MAP、SV、CO、SVR、CCE、dp/dt高于B组,差异有统计学意义(P<0.05)。结论环泊酚在体外循环下二尖瓣置换术麻醉诱导时期能较好地保持血流动力学稳定,降低心功能损害。

关键词：环泊酚诱导二尖瓣置换血流动力学

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宁波市定点医院丙型肝炎病毒感染者基因型分布及感染途径分析

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中国预防医学杂志 2024年第7期25卷 867-872页

作者：蒋素文练祥胡爱荣胡宇涛汪东辉周爱武范莹林恳王家岚宁波市第二医院浙江宁波315010 宁波市第四医院绍兴文理学院医学院宁波大学医学部温州医科大学

目的分析宁波市两家定点医院丙型肝炎病毒(hepatitis C virus,HCV)慢性感染者病毒基因型和感染途径分布,为丙型肝炎的防治提供依据。方法纳入2011年4月—2021年12月在宁波市第二医院及宁波市第四医院确诊为慢性HCV感染者且基因型明确,... 详细信息

目的分析宁波市两家定点医院丙型肝炎病毒(hepatitis C virus,HCV)慢性感染者病毒基因型和感染途径分布,为丙型肝炎的防治提供依据。方法纳入2011年4月—2021年12月在宁波市第二医院及宁波市第四医院确诊为慢性HCV感染者且基因型明确,分析不同检测时间、年龄和感染途径人群HCV基因型分布。结果调查HCV感染者1342例,基于定点医院就诊者的数据,HCV基因型检出数量(比例)呈逐年增长趋势,2020年达高峰192例(14.31%),2021年降至135例(10.06%)。HCV感染前3位的基因型分别为1型659例(49.11%)、6型268例(19.97%)和3型208例(15.50%)。每年仍以HCV基因型1型为主,但2015年之后3型和6型的所占比例总体呈增加趋势,后期组中3型及6型的构成比(18.07%和27.10%)均高于前期组(11.87%和9.89%),差异有统计学意义(χ^(2)=9.544、60.329,P20~60岁)为主,1179例(87.85%);各年龄段间1型、2型、3型及6型的构成比差异有统计学意义(χ^(2)=112.986,P50~60岁组的占比(64.42%)最高,2型在>60岁组的占比(16.13%)最高,3型、6型在>30~40岁年龄段中的占比(26.01%和26.59%)最高。能明确感染途径的患者345例,前3位为静脉药瘾86例、有偿献血史61例和不安全的针刺、注射、口腔诊疗57例;不同感染途径患者的年龄及性别总体比较差异有统计学意义(F=16.761,P<0.01;χ^(2)=38.278,P<0.01)。结论宁波市两定点医院就诊者HCV基因型分布呈多样性;1型、6型和3型为前3位基因型,不同基因型感染者的年龄、性别和感染途径等存在差异。

关键词：丙型肝炎病毒丙型肝炎基因型感染途径

The Application of Quantitative Fluorescence Techniques in China

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Global Geology 2000年第2期5卷 220-222页

作者： hu yutao Yang Haibo Guo Qingxia No. 5 Production Plant Daqing 163000 P. R. China Daqing Petroleum Administrative Bureau Daging 163453 P. R. China Daqing Geoloyging Company Daqing 163411 P. R. China

The introduction of multi-methods of Geo-logging at wellsite has become the major measurement of oil & gas exploration. From the early stage of manually geo-logging to the modern mudlogging with new techonlogies of MWD, LWD and QFT etc. The new technologies have played very important roles in the exploring of oil & gas. Being one of the newest technology of mudlogging, QFT has been widely used in oilfield for about 3 years. When it is put in operation in some oilfields of China in 1997, its advantages in oil & gas detection at wellsite have been continuously recognized,especially in the detection of shows of light oil and condensed oil. A set of powerful classification standard of resource rock oil bearing grades and the interpretation standards have been summarized by the application of the quantitative fluorescencelogging techniques (QFT) in Basins of China, together with gas-logging data, and other information got from the Geo-logging procedures at wellsite.

关键词： Quantitative fluorescence Application Techniques

Natural Gas Resource in Jurassic,Y Faulted Subbasin of Songliao Basin

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Global Geology 2000年第2期5卷 202-206页

作者： Yang Haibo hu yutao Wei Xinghua Daqing Petroleum Administrative Bureau Daqing 163453 P. R. China No. 5 production plant Daqing 163000 P. R. China

Y faulted depression is located in the southeast of Songliao basin, which is another important area explored for natural gas in deep Formation after Xujiaweizi. But it is lowly explored and is not likely to make a major breakthrough in nature gas exploration for shallow and medium layers. The data on the geochemistry and geology indicate that the deep source rocks, especially the Jurassic mudstone has great potentials of natural gas generation. Based on this, the paper examines the conditions of natural gas generation and further points out the favorable prospective gas - bearing area. The results show that the amount of natural gas generated from the Jurassic mudstone in the Y faulted depression is 8. 42x1012m3 and the favorable gas - generating area is the body of the faulted depression.

关键词： Y faulted depression Deep strata Oil and gas resource

Wellsite Evaluation Method of Undercompacted Formation

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Global Geology 2000年第2期5卷 210-216页

作者： Guo Qingxia Wei Xinghua hu yutao Yang Haibo Daqing Geologging Company Daqing 163411 P. R. China Daqing Petroleum Administrative Bureau Daqing 163453 P. R. China No. 5 Production Plant Daqing 163000 P. R. China

Staring from the view point of practical application, based on large amount of pressure well - logging data home and abroad, the author demonstrated how to distinguish overpressure Formation using wellsite data through selected methods such as drilling parameters, rock cutting analysis, drilling fluid parameters, and electric welline logs.

关键词： Formation pressure Drilling parameters Rock fragment Drilling fluid Well - logging

A novel FGFR1 inhibitor CYY292 suppresses tumor progression,invasion,and metastasis of glioblastoma by inhibiting the Akt/GSK3β/snail signaling axis

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Genes & Diseases 2024年第1期11卷 479-494页

作者： Yanran Bi Ruiling Zheng Jiahao hu Ruiqing Shi Junfeng Shi yutao Wang Peng Wang Wenyi Jiang Gyudong Kim Zhiguo Liu Xiaokun Li Li Lin School of Pharmaceutical Science Wenzhou Medical UniversityWenzhouZhejiang 325035China College of Pharmacy and Research Institute of Drug Development Chonnam National UniversityGwangju 61186Republic of Korea Department of Dermatology Wenzhou Hospital of Integrated Traditional Chinese and Western MedicineWenzhouZhejiang 325000China Zhejiang Lab for Regenerative Medicine Oujiang LaboratoryWenzhouZhejiang 325035China

Glioblastoma(GBM)is a malignant brain tumor that grows quickly,spreads widely,and is resistant to *** growth factor receptor(FGFR)1 is a receptor tyrosine kinase that regulates cellular processes,including proliferation,survival,migration,and ***1 was predominantly expressed in GBM tissues,and FGFR1 expression was negatively correlated with overall *** rationally designed a novel small molecule CYY292,which exhibited a strong affinity for the FGFR1 protein in GBM cell lines in ***292 also exerted an effect on the conserved Ser777 residue of ***292 dose-depen-dently inhibited cell proliferation,epithelial-mesenchymal transition,stemness,invasion,and migration in vitro by specifically targeting the FGFR1/AKT/Snail pathways in GBM cells,and this effect was prevented by pharmacological inhibitors and critical gene *** vivo experiments revealed that CYY292 inhibited U87MG tumor growth more effectively than ***292 also efficiently reduced GBM cell proliferation and increased survival in orthotopic GBM *** study further elucidates the function of FGFR1 in the GBM and reveals the effect of CYY292,which targets FGFR1,on downstream signaling pathways directly reducing GBM cell growth,invasion,and metastasis and thus impairing the recruitment,activation,and function of immune cells.

关键词： Blood-brain barrier EMT FGFR1 FGFR1 inhibitor Glioblastoma