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Sequencing and analysis of the complete mitochondrial DNA of Russell's snapper (***)

Progress in Natural science:Materials International 2008年第10期18卷 1233-1238页

作者： yusong guo~(a,1) {2. wang~(a,b,1) chuwu liu~(a,*) yun liu~b ~a fisheries College,guangdong ocean university,zhanjiang 52.02.,china ~b College of {12. science,hunan normal university,changsha 410081,china fisheries College Guangdong Ocean University Zhanjiang 524025 China College of life science Hunan Normal University Changsha 410081 China

The entire mitochondrial DNA sequence (mitogenome) of Russell’s snapper (Lutjanus russellii) was determined using long PCR and primer-walking methodology,representing the first complete mitogenome accessioned for Lutjanid fishes (16,505 bp,Genbank Acces- sion ***5142.8).The mitogenome was similar in gene composition and order to those of other vertebrates,having 37 structural genes,i.e.,two ribosomal RNAs,2. transfer RNAs,and 13 protein-coding *** analyses based on the mtDNA sequence of Russell’s snapper supported a close relationship between Lutjaninae and Caesioninae,consistent with taxonomic hypotheses based on *** studies utilizing mitogenomes are needed to resolve high-level relationships among snappers.

关键词： Lutjanus russellii Mitochondrial DNA Mitogenome Complete mtDNA sequence Long PCR

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DNA barcoding, phylogenetic relationships and speciation of snappers (genus Lutjanus)

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science china({2. sciences) 2010年第8期53卷 1025-1030页

作者： wang zhongduo1,2.3, {2. yusong1,3, TAN Wei1, LI Lu1, TANG EnPu1, liu chuwu1,2.3 & liu yun2.1fisheries College, guangdong ocean university, zhanjiang 52.02., china 2.ollege of {12. science, hunan normal university, changsha 410081, china 3Key Laboratory of Aquaculture in South china Sea for Aquatic Economic Animal of guangdong Higher Education Institutes, zhanjiang 52.02., china 1. fisheries College Guangdong Ocean University Zhanjiang 524025 China2. College of Life Science Hunan Normal University Changsha 410081 China3. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes Zhanjiang 524025 China

The phylogenetic relationships of 13 snapper species from the South china Sea have been established using the combined DNA sequences of three full-length mitochondrial genes (COI, COII and CYTb) and two partial nuclear genes (RAG1, RAG2.. The 13 species (genus Lutjanus) were selected after DNA barcoding 72.individuals, representing 2. species. Our study suggests that although DNA barcoding aims to develop species identification systems, it may also be useful in the construction of phylogenies by aiding the selection of taxa. Combined mitochondrial and nuclear gene data has an advantage over an individual dataset because of its higher resolving power.

关键词： phylogenetic relationship Lutjanus speciation DNA barcoding

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