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Journal of Integrative Plant Biology 2011年第9期53卷 698-709页

作者： Cheng Du yunyuan xu Yingdian Wang Kang Chong Key Laboratory of Photosynthesis and Molecular Environmental Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China College of Life Sciences Beijing Normal UniversityBeijing 100875China

Polar auxin transport,which depends on polarized subcellular distribution of AUXIN RESISTANT 1/LIKE AUX1（AUX1/LAX） influx carriers and PIN-FORMED（PIN） efflux carriers,mediates various processes of plant growth and *** recycling of PIN1 is mediated by an adenosine diphosphate（ADP）ribosylation factor（ARF）-GTPase exchange factor protein,***,the mediation of auxin influx carrier recycling is poorly ***,we report that overexpression of OsAGAP,an ARF-GTPase-activating protein in rice,stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and ***1 endosomes could partially colocalize with FM4-64 labeled early endosome after actin ***,OsAGAP is involved in actin cytoskeletal organization,and its overexpression tends to reduce the thickness and bundling of actin *** recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells,and was only slightly promoted when the actin filaments were completely disrupted by Lat ***,we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption.

关键词： actin adenosine diphosphate ribosylation factor-GTPase-activating protein AUX1 endosome polar auxin transport.

Transcriptome-wide Analysis Of Vernalization Reveals Conserved and Species-specific Mechanisms in Brachypodium

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Journal of Integrative Plant Biology 2013年第8期55卷 696-709页

作者： Qing Huan Zhiwei Mao Jingyu Zhang yunyuan xu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of Sciences University of Chinese Academy of Sciences the Chinese Academy of Sciences

Several temperate cereals need vernalization to promote flowering. Little, however, is known about the vernalization-memory-related genes, and almost no comparative analysis has been performed. Here, RNA- Seq was used for transcriptome analysis in non-vernalized, vernalized and post-vernalized Brachypodium distachyon （L.） Beauv. seedlings. In total, the expression of 1,665 genes showed significant changes （fold change 〉4） in response to vernalization. Among them, 674 putative vernalization-memory-related genes with a constant response to vernalization were significantly enriched in transcriptional regulation and monooxygenase-mediated biological processes. Comparative analysis of vernalization-memory-related genes with barley demonstrated that the oxidative-stress response was the most conserved pathway between these two plant species. Moreover, Brachypodium preferred to regulate transcription and protein phosphorylation processes, while vernalization-memory-related genes, whose products are cytoplasmic membrane-bound-vesicle-located proteins, were preferred to be regulated in barley. Correlation analysis of the vernalization-related genes with barley revealed that the vernalization mechanism was conserved between these two plant species. In summary, vernalization, including its memory mechanism, is conserved between Brachypodium and barley, although several species-specific features also exist. The data reported here will provide primary resources for subsequent functional research in vernalization.

关键词： Brachypodium comparative analysis RNA-Seq vernalization.

The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat

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Molecular Plant 2021年第9期14卷 1525-1538页

作者： Shujuan xu Qi Dong Min Deng Dexing Lin Jun Xiao Peilei Cheng Lijing Xing Yuda Niu Caixia Gao Wenhao Zhang yunyuan xu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Innovation Academy for Seed Design Chinese Academy of SciencesBeijing 100093China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Plant Cell and Chromosome Engineering Center for Genome EditingInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China College of Horticulture Nanjing Agricultural UniversityKey Laboratory of LandscapingMinistry of AgricultureNanjing 210095China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Vernalization is a physiological process in which prolonged cold exposure establishes flowering competence in winter plants. In hexaploid wheat, TaVRN1 is a cold-induced key regulator that accelerates floral transition. However, the molecular mechanism underlying the gradual activation of TaVRN1 during the vernalization process remains unknown. In this study, we identified the novel transcript VAS (TaVRN1 alternative splicing) as a non-coding RNA derived from the sense strand of the TaVRN1 gene only in winter wheat, which regulates TaVRN1 transcription for flowering. VAS was induced during the early period of vernalization, and its overexpression promoted TaVRN1 expression to accelerate flowering in winter wheat. VAS physically associates with TaRF2b and facilitates docking of the TaRF2b-TaRF2a complex at the TaVRN1 promoter during the middle period of vernalization. TaRF2b recognizes the Sp1 motif within the TaVRN1 proximal promoter region, which is gradually exposed along with the disruption of a loop structure at the TaVRN1 locus during vernalization, to activate the transcription of TaVRN1. The tarf2b mutants exhibited delayed flowering, whereas transgenic wheat lines overexpressing TaRF2b showed earlier flowering. Taken together, our data reveal a distinct regulatory mechanism by which a long non-coding RNA facilitates the transcription factor targeting to regulate wheat flowering, providing novel insights into the vernalization process and a potential target for wheat genetic improvement.

关键词： winter wheat vernalization flowering long non-coding RNA chromosome loop

OsCIPK7 point-mutation leads to conformation and kinase-activity change for sensing cold response

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Journal of Integrative Plant Biology 2019年第12期61卷 1194-1200页

作者： Dajian Zhang Xiaoyu Guo yunyuan xu Hao Li Liang Ma xuefeng Yao Yuxiang Weng Yan Guo Chun-Ming Liu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Soft Matter Physics Institute of Physicsthe Chinese Academy of SciencesBeijing 100190China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Calcineurin B-like interacting protein kinases(CIPKs) play important roles via environmental ***, less is known how to sense the stress in molecular structure conformation level. Here, an Os CIPK7 mutant via TILLING procedure with a point mutation in the kinase domain showed increased chilling tolerance, which could be potentially used in the molecular breeding. We found that this point mutation of Os CIPK7 led to a conformationalchange in the activation loop of the kinase domain,subsequently with an increase of protein kinase activity,thus conferred an increased tolerance to chilling stress.

关键词： conformation conformational activation

The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice

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Molecular Plant 2021年第10期14卷 1699-1713页

作者： Wensi Yang Kun Wu Bo Wang Huanhuan Liu Siyi Guo Xiaoyu Guo Wei Luo Shengyuan Sun Yidan Ouyang Xiangdong Fu Kang Chong Qifa Zhang yunyuan xu Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China University of Chinese Academy of Sciences Beijing 100049China The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Innovation Academy for Seed Design CASBeijing 100101China

G-protein signaling and ubiquitin-dependent degradation are both involved in grain development in rice,but how these pathways are coordinated in regulating this process is ***,we show that Chang Li Geng 1(CLG1),which encodes an E3 ligase,regulates grain size by targeting the Gγprotein GS3,a negative regulator of grain length,for *** of CLG1 led to increased grain length,while overexpression of mutated CLG1 with changes in three conserved amino acids decreased grain *** found that CLG1 physically interacts with and ubiquitinats GS3which is subsequently degraded through the endosome degradation pathway,leading to increased grain ***,we identified a critical SNP in the exon 3 of CLG1 that is significantly associated with grain size variation in a core collection of cultivated *** SNP results in an amino acid substitution from Arg to Ser at position 163 of CLG1 that enhances the E3 ligase activity of CLG1 and thus increases rice grain *** the expression level of CLG1 and the SNP CLG1163S may be useful variations for manipulating grain size in rice.

关键词： grain size CLG1 GS3 E3 ligase endosome

O-linked β-N-acetylglucosamine modification and its biological functions

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Science Bulletin 2015年第12期60卷 1055-1061,I0002页

作者： Yan Liu Shaojun Dai Lijing Xing yunyuan xu Kang Chong Alkali Soil Natural Environmental Science Center NortheastForestry University Key Laboratory of Saline-Alkali VegetationEcology Restoration in Oil Field Ministry of Education Harbin150040 China Key Laboratory of Plant Molecular Physiology Institute ofBotany Chinese Academy of Sciences Beijing 100093 China

The covalent attachment of O-linked β-N- acetylglucosamine （O-GIcNAc） to Ser/Thr residues of proteins acts as not only a posttranslational modification but also a nutritional sensor in nucleus and cytoplasm, which directly regulates the expression of genes and multiple crucial signal transduction pathways. Dynamic O- GlcNAcylation at Ser/Thr residues is catalyzed by two key enzymes, O-GIcNAc transferase （OGT） and O-GlcNAcase, which are responsible for addition and removal of the O- GlcNAc modification, respectively. O-GlcNAc modifica- tion plays important roles in cellular signaling in animals, especially in human diseases. Two orthologs of OGT in plants, SECRET AGENT and SPINDLY, have been reported to be involved in diverse plant processes. However, compared with the functional mechanisms revealed in animals, the consequences of protein O-GlcNAc modifi- cation in plants is largely unknown, and the relationship between O-GlcNAcylation and cellular processes needs to be explored. In this review, we summarized the recent advances on O-GlcNAc modification and its biological functions in animals and plants, and prospect of more special functions of O-GlcNAc will be revealed in plants.

关键词： O-GlcNAc Posttranslational modification Epigenetic regulation Plants

Deep Sequencing of Brachypodium Small RNAs at the Global Genome Level Identifies MicroRNAs involved in Cold Stress Response

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Deep Sequencing of Brachypodium Small RNAs at the Global Gen...

第十二届全国植物基因组学大会

作者： Jingyu Zhang,yunyuan xu,Qing Huan,Kang Chong~* 中科院植物所

High-throughput sequencing and whole-genome-wide data mining led to the identification of 27 conserved miRNAs,as well as 129 predicted miRNAs in *** expression of 3 conserved miRNAs and 25 predicted miRNAs showed significant changes in response to cold *** these miRNAs, some were cold-induced and some were cold-suppressed,but all the conserved miRNAs were up-regulated under cold stress *** results suggest that Brachypodium miRNAs are composed of a set of

关键词：

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平板型空间网架抗震分析

同济大学学报 1983年第1期 44-57页

作者：陈扬骥徐运源

本文建立了在三维地震力作用下平板型空间网架的振动方程,采用子空间迭代法求网架自振频率和振型。并列出了电算程序的框图。利用此程序分析了十四个不同型式、不同跨度的网架的地震反应,用回归分析方法得出竖向荷载系数C_Z值的计算公式。

关键词：自振频率固有频率竖向地震荷载杆件内力网架结构抗震分析结构分析地震反应振型质量矩阵子空间迭代法振动方程地震波空间网架平板型

The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling

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Cell Research 2007年第6期17卷 458-470页

作者： Haili Dong1,2 Yan Deng1,2 Jinye Mu1 Qingtao Lu3 Yiqin Wang1 yunyuan xu3 Chengcai Chu1 Kang Chong3 Congming Lu3 Jianru Zuo1 1 State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beoing 100101, China 2 Graduate School, Chinese Academy of Sciences, Beijing 100039, China 3 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofSciencesBeijing100101China GraduateSchoolChineseAcademyofSciences Beijing

Carotenoids, a class of natural pigments found in all photosynthetic organisms, are involved in a variety of physiologi-cal processes, including coloration, photoprotection, biosynthesis of abscisic acid (ABA) and chloroplast *** carotenoid biosynthesis has been well studied biochemically, the genetic basis of the pathway is not wellunderstood. Here, we report the characterization of two allelic Arabidopsis mutants, spontaneous cell death 1-1 (spcl-1)and spcl-2. The weak allele spcl-1 mutant showed characteristics of bleached leaves, accumulation of superoxide andmosaic cell death. The strong mutant allele spcl-2 caused a complete arrest of plant growth and development shortlyafter germination, leading to a seedling-lethal phenotype. Genetic and molecular analyses indicated that SPC1 encodesa putative ζ-carotene desaturase (ZDS) in the carotenoid biosynthesis pathway. Analysis of carotenoids revealed thatseveral major carotenoid compounds downstream of SPC1/ZDS were substantially reduced in spcl-1, suggesting thatSPC1 is a functional ZDS. Consistent with the downregulated expression of CAO and PORB, the chlorophyll contentwas decreased in spcl-1 plants. In addition, expression of Lhcb1.1, Lhcb1.4 and RbcS was absent in spcl-2, suggestingthe possible involvement of carotenoids in the plastid-to-nucleus retrograde signaling. The spcl-1 mutant also displaysan ABA-deficient phenotype that can be partially rescued by the externally supplied phytohormone. These results suggestthat SPC1/ZDS is essential for biosynthesis of carotenoids and plays a crucial role in plant growth and development.

关键词： carotenoid biosynthesis cell death chloroplast development photoprotection Arabidopsis SPC1/ZDS

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利用低温水浴鉴定水稻苗期耐寒性

植物学报 2019年第4期54卷 509-514页

作者：刘栋峰唐永严雒胜韬罗伟李志涛种康徐云远中国科学院植物研究所植物分子生理学重点实验室北京100093

高效准确鉴定苗期耐寒性是水稻(Oryza sativa)耐寒研究的前提。基于流动水浴温度均一这一特性,建立了一种恒温水浴鉴定水稻幼苗耐寒性方法。该方法中环境温度设定为20°C,水浴温度设定为4°C。根据对2个水稻亚种不同材料的处理结果,总... 详细信息

高效准确鉴定苗期耐寒性是水稻(Oryza sativa)耐寒研究的前提。基于流动水浴温度均一这一特性,建立了一种恒温水浴鉴定水稻幼苗耐寒性方法。该方法中环境温度设定为20°C,水浴温度设定为4°C。根据对2个水稻亚种不同材料的处理结果,总结出几种常见品种的低温处理时间与存活率参考值,并对操作过程中的一些注意事项进行了说明。

关键词：水稻苗期耐寒低温水浴