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Battle of the sexes: contrasting roles of testis-specific protein Y-encoded (TSPY) and TSPX in human oncogenesis

Asian Journal of Andrology 2019年第3期21卷 260-269页

作者： Yun-Fai Chris Lau yunmin li Tatsuo Kido Division of Cell and Developmental Genetics Department of Medicine VA Medical Center and Institute for Human Genetics University of California San Francisco CA 94121 USA

The Y-located testis-specific protein Y-encoded (TSPY) and its X-homologue TSPX originated from the same ancestral gene, but act as a proto-oncogene and a tumor suppressor gene, respectively. TSPY has specialized in male-specific functions, while TSPX has assumed the functions of the ancestral gene. Both TSPY and TSPX harbor a conserved SET/NAP domain, but are divergent at flanking structures. Specifically, TSPX contains a C-terminal acidic domain, absent in TSPY. They possess contrasting properties, in which TSPY and TSPX, respectively, accelerate and arrest cell proliferation, stimulate and inhibit cyclin B-CDK1 phosphorylation activities, have no effect and promote proteosomal degradation of the viral HBx oncoprotein, and exacerbate and repress androgen receptor (AR) and constitutively active AR variant, such as AR-V7, gene transactivation. The inhibitory domain has been mapped to the carboxyl acidic domain in TSPX, truncation of which results in an abbreviated TSPX exerting positive actions as TSPY. Transposition of the acidic domain to the C-terminus of TSPY results in an inhibitory protein as intact TSPX. Hence, genomic mutations/aberrant splicing events could generate TSPX proteins with truncated acidic domain and oncogenic properties as those for TSPY. Further, TSPY is upregulated by AR and AR-V7 in ligand-dependent and ligand-independent manners, respectively, suggesting the existence of a positive feedback loop between a Y-located proto-oncogene and male sex hormone/receptors, thereby amplifying the respective male oncogenic actions in human cancers and diseases. TSPX counteracts such positive feedback loop. Hence, TSPY and TSPX are homologues on the sex chromosomes that function at the two extremes of the human oncogenic spectrum.

关键词： androgen receptor AR-V7 cell cycle regulation cyclin B-CDK1 oncogene sex chromosomes sex differences TSPX TSPY tumor suppressor

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Investigation on mechanical properties regulation of rock-like specimens based on 3D printing and similarity quantification

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International Journal of Mining Science and Technology 2024年第5期34卷 573-585页

作者： Duanyang Zhuang Zexu Ning yunmin Chen Jinlong li Qingdong li Wenjie Xu Center for Hypergravity Experiment and Interdisciplinary Research Zhejiang UniversityHangzhou 310058China MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering Zhejiang UniversityHangzhou 310058China

3D printing is widely adopted to quickly produce rock mass models with complex structures in batches,improving the consistency and repeatability of physical *** is necessary to regulate the mechanical properties of 3D-printed specimens to make them proportionally similar to natural *** study investigates mechanical properties of 3D-printed rock analogues prepared by furan resin-bonded silica sand *** mechanical property regulation of 3D-printed specimens is realized through quantifying its similarity to sandstone,so that analogous deformation characteristics and failure mode are *** similarity conversion,uniaxial compressive strength,cohesion and stress–strain relationship curve of 3D-printed specimen are similar to those of *** the study ranges,the strength of 3D-printed specimen is positively correlated with the additive content,negatively correlated with the sand particle size,and first increases then decreases with the increase of curing *** regulation scheme with optimal similarity quantification index,that is the sand type of 70/140,additive content of 2.5‰and curing temperature of 81.6℃,is determined for preparing 3D-printed sandstone analogues and *** effectiveness of mechanical property regulation is proved through uniaxial compression contrast *** study provides a reference for preparing rock-like specimens and engineering models using 3D printing technology.

关键词： 3D printing Mechanical property regulation Similarity quantification Rock analogue Sandstone

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Developmental staging of male murine embryonic gonad by SAGE analysis

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Journal of Genetics and Genomics 2009年第4期36卷 215-227页

作者： Tin-Lap Lee yunmin li Diana Alba Queenie R Vong Shao-Ming Wu Vanessa Baxendale Owen M. Rennert Yun-Fai Chris Lau Wai-Yee Chan Section on Developmental Genomics Laboratory of Clinical Genomics Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Bethesda MD 20892 USA Department of Medicine VA Medical Center University of California San Francisco CA 94121 USA Department of Pediatrics Biochemistry Molecular & Cellular Biology Georgetown University Washington DC 20007 USA

Despite the identification of key genes such as Sry integral to embryonic gonadal development, the genomic classification and identification of chromosomal activation of this process is still poorly understood. To better understand the genetic regulation of gonadal development, we performed Serial Analysis of Gene Expression （SAGE） to profile the genes and novel transcripts, and an average of 152,000 tags from male embryonic gonads at E10.5 （embryonic day 10.5）, E11.5, E12.5, E13.5, E15.5 and E17.5 were analyzed. A total of 275,583 non-singleton tags that do not map to any annotated sequence were identified in the six gonad libraries, and 47,255 tags were mapped to 24,975 annotated sequences, among which 987 sequences were uncharacterized. Utilizing an unsupervised pattern identification technique, we established molecular staging of male gonadal development. Rather than providing a static descriptive analysis, we developed algorithms to cluster the SAGE data and assign SAGE tags to a corresponding chromosomal position; these data are displayed in chromosome graphic format. A prominent increase in global genomic activity from E10.5 to E17.5 was observed. Important chromosomal regions related to the developmental processes were identified and validated based on established mouse models with developmental disorders. These regions may represent markers for early diagnosis for disorders of male gonad development as well as potential treatment targets.

关键词： SAGE transcriptome male gonads gene tag novel transcripts cluster analysis chromosome transcription hotspot

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Feasibility analysis of hypergravity experiment of density-driven convection of dissolved CO

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Journal of Earth Science 2024年

作者： Ruiqi Chen Wenjie Xu Yingtao Hu yunmin Chen Jinlong li Duanyang Zhuang Center for Hypergravity Experimental and Interdisciplinary Research， Zhejiang University MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering， Zhejiang University School of Engineering， Hangzhou City University

Dissolution trapping is one of the most promising mechanisms for safe geological carbon storage. Density-driven convection substantially accelerates the conversion of free-phase CO2to the dissolved state， enhancing the sequestration safety. Since this process occurs on time scales of hundreds to thousands of years， reproducing it through conventional laboratory physical model tests is challenging. The hypergravity experiment reduces the model size and shortens the experimental time， enabling the modeling of gravity-driven flow processes at the field scale. However， it is uncertain whether the preferential flow effect caused by fractures can be reproduced in a hypergravity experiment. In this study， a three-dimensional discrete fracture-matrix model （3D-DFM） was used to evaluate the feasibility of hypergravity experiment of the transport of dissolved CO2in fractured *** hypergravity tests were performed to examine the feasibility of modeling density-driven convection in homogeneous and heterogeneous media at different centrifuge accelerations. The hypergravity experiment can be used to study density-driven convection of dissolved CO2at the field scale in homogeneous system. The numerical results show that the hypergravity experiment enables a faster breakthrough of plume and overestimates CO2migration in the matrix surrounding the fractures.

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机械通气并发呼吸道感染的临床分析

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亚太传统医药 2007年第11期3卷 89-90页

作者：李云敏瑞安市人民医院浙江瑞安325200

目的：分析机械通气并发下呼吸道感染的病原学及药敏情况，探讨有效的护理预防措施。方法：回顾分析我院2004年1月-2007年1月三年间重症监护病房（ICU）机械通气并发下呼吸道感染的情况。结果：机械通气并发下呼吸道感染率为78．1％，... 详细信息

目的：分析机械通气并发下呼吸道感染的病原学及药敏情况，探讨有效的护理预防措施。方法：回顾分析我院2004年1月-2007年1月三年间重症监护病房（ICU）机械通气并发下呼吸道感染的情况。结果：机械通气并发下呼吸道感染率为78．1％，主要病原菌是革兰氏阴性杆菌、革兰氏阳性球菌和真菌；各种菌类对相应的抗生素都比较敏感。结论：机械通气并发下呼吸道感染以革兰氏阴性杆菌为主，应加强人工气道护理和消毒隔离工作，根据药敏试验结果选用有效的抗菌药物是，临床医生用药的关键。

关键词：机械通气下呼吸道感染药敏 ICU 革兰氏阴性杆菌

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结构性软土压缩特性研究

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土木工程学报 2004年第4期37卷 46-53页

作者：王立忠丁利陈云敏李玲玲浙江大学建筑工程学院

针对结构性软土的压缩性 ,通过对温州软土的原状土和重塑土在不同加压率下的固结试验 ,分析原状土和重塑土不同的压缩性状以及加压率对土体压缩性状的影响。在此基础上对已有的原位压缩曲线确定方法进行探讨 ,并结合对世界各地土样的试... 详细信息

针对结构性软土的压缩性 ,通过对温州软土的原状土和重塑土在不同加压率下的固结试验 ,分析原状土和重塑土不同的压缩性状以及加压率对土体压缩性状的影响。在此基础上对已有的原位压缩曲线确定方法进行探讨 ,并结合对世界各地土样的试验室压缩曲线的分析 ,提出新的折线形原位压缩曲线确定方法。

关键词：结构性土体压缩性加压率原位压缩曲线

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艾司西酞普兰联合氨磺必利治疗老年抑郁症患者的效果

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实用临床医药杂志 2020年第9期24卷 27-29页

作者：廉拥军李云敏陕西省铜川矿务局精神卫生康复中心精神科陕西铜川727008

目的探讨艾司西酞普兰联合氨磺必利治疗65岁以上抑郁症患者的效果及对负性情绪的影响。方法回顾性分析190例65岁以上抑郁症患者的临床资料,并按治疗方案不同分为2组。将单纯应用艾司西酞普兰治疗的患者设为对照组(n=95),将采取艾司西酞... 详细信息

目的探讨艾司西酞普兰联合氨磺必利治疗65岁以上抑郁症患者的效果及对负性情绪的影响。方法回顾性分析190例65岁以上抑郁症患者的临床资料,并按治疗方案不同分为2组。将单纯应用艾司西酞普兰治疗的患者设为对照组(n=95),将采取艾司西酞普兰联合氨磺必利治疗的患者设为研究组(n=95)。比较2组治疗效果、治疗前后负性情绪变化以及副反应发生率。结果研究组患者的抑郁症总体治疗有效率为97.89%,显著高于对照组的90.53%(P<0.05);治疗后,研究组和对照组的症状自评量表(SCL-90)评分均显著低于治疗前,且研究组SCL-90评分显著低于对照组(P<0.05);研究组患者的副反应发生率为4.21%,显著低于对照组的13.68%(P<0.05)。结论艾司西酞普兰联合氨磺必利治疗65岁以上抑郁症患者的临床效果显著,可有效消除患者负性情绪,且安全性较高。

关键词：艾司西酞普兰氨磺必利抑郁症老年患者不良反应负性情绪

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合肥光源40kW高频固态发射机的研制进展

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强激光与粒子束 2010年第10期22卷 2417-2420页

作者：黄贵荣初晨金凯王金祥贾大春贺正孙永惠李云敏中国科学技术大学国家同步辐射实验室合肥230029 北京北广科技股份有限公司北京100016

介绍了用于合肥光源800 MeV电子储存环高频系统的204 MHz,40 kW固态发射机的研制进展。该发射机为双塔结构,由130个330 W功放模块进行三级合成。其关键部件的样件包括10路功放和2.5kW八合一,20 kW八合一以及40 kW二合一功率合成器各1件... 详细信息

介绍了用于合肥光源800 MeV电子储存环高频系统的204 MHz,40 kW固态发射机的研制进展。该发射机为双塔结构,由130个330 W功放模块进行三级合成。其关键部件的样件包括10路功放和2.5kW八合一,20 kW八合一以及40 kW二合一功率合成器各1件的制作和测试已完成。测得各合成器输出端驻波比不超过1.05,功放模块的增益达到26 dB,幅相偏差小于0.1 dB和5°,达到设计要求。

关键词：高频系统固态发射机径向合成器高频功放模块

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眼科临床教学中医患沟通教育的探讨

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全科医学临床与教育 2006年第5期4卷 402-404页

作者：刘曦李毓敏王碧浪浙江大学医学院附属一院眼科浙江杭州310003

眼科临床实习是医学生临床实习的重要组成部分.医学生临床实习的过程是把理论知识运用到具体患者的诊断和治疗上.医学生临床实习的中心是患者,医疗工作的宗旨是为病人服务.在医疗工作中,医生既要注意患者身体的疾病,又要关注病人的心理... 详细信息

眼科临床实习是医学生临床实习的重要组成部分.医学生临床实习的过程是把理论知识运用到具体患者的诊断和治疗上.医学生临床实习的中心是患者,医疗工作的宗旨是为病人服务.在医疗工作中,医生既要注意患者身体的疾病,又要关注病人的心理和情感.解决好如何与患者沟通,使得病人能积极配合医生的检查和治疗,建立医患之间相互尊重、相互信任的友好关系是现代医学模式对临床医学教学的新要求,也是临床医学教育改革的新内容.

关键词：医患沟通教育临床教学眼科学生临床实习为病人服务临床医学教学现代医学模式医学教育改革

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Molecular force mechanism of hydrodynamics in clay nanopores

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浙江大学学报(英文版)A辑:应用物理与工程 2023年第9期24卷 817-827页

作者： Shengjie WEI Yuchao li Peng SHEN yunmin CHEN MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering College of Civil Engineering and ArchitectureZhejiang UniversityHangzhou 310058China

Nanopores are prevalent within various clay morphologies,and water flow in clay nanopores is significant for various engineering *** this study,we performed non-equilibrium molecular dynamics(NEMD)simulations to reveal the molecular force mechanisms of water flow in clay *** water dynamic viscosity,slip length,and average flow velocity were obtained to verify the NEMD *** the water confined in the nanopores maintained a dynamic mechanical equilibrium state,each water lamina can be regarded as a simply supported *** applied driving force,the force from clay crystal layers,the force from compensating sodium ions,and the force from other water laminae were further calculated to investigate the force *** van der Wals barrier above the surface and hydraulic gradient lead to distribution differences in water oxygen atoms,which contribute to a net van der Waals resistance component of the force from clay crystal ***,the water molecules tend to rotate to generate the electrostatic resistance component of the force from clay crystal layers and balance the increasing hydraulic *** to the velocity difference,the water molecules in the slower lamina have a higher tendency to lag and generate a net electrostatic resistance force as well as a net van der Waals driving force on the water molecules in the faster lamina,which together make up the viscous force.

关键词： Molecular dynamics Hydrodynamics Clay Nanopore Molecular force Boundary effect Viscous force

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