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Dynamic Change of Genetic Diversity in Conserved Populations with Different Initial Genetic Architectures

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Journal of Integrative Agriculture 2013年第7期12卷 1225-1233页

作者： lu yun-feng LI Hong-wei WU Ke-liang WU Chang-xin College of Animal Science and Technology China Agricultural University School of Life Science and Technology Nanyang Normal University National Engineering Laboratory for Animal Breeding

Maintenance and management of genetic diversity of farm animal genetic resources （AnGR） is very important for biological, socioeconomical and cultural significance. The core concern of conservation for farm AnGR is the retention of genetic diversity of conserved populations in a long-term perspective. However, numerous factors may affect evolution of genetic diversity of a conserved population. Among those factors, the genetic architecture of conserved populations is little considered in current conservation strategies. In this study, we investigated the dynamic changes of genetic diversity of conserved populations with two scenarios on initial genetic architectures by computer simulation in which thirty polymorphic microsatellite loci were chosen to represent genetic architecture of the populations with observed heterozygosity （Ho） and expected heterozygosity （He）, observed and mean effective number of alleles （Ao and Ae）, number of polymorphic loci （NP） and the percentage of polymorphic loci （PP）, number of rare alleles （RA） and number of non-rich polymorphic loci （NRP） as the estimates of genetic diversity. The two scenarios on genetic architecture were taken into account, namely, one conserved population with same allele frequency （AS） and another one with actual allele frequency （AA）. The results showed that the magnitude of loss of genetic diversity is associated with genetic architecture of initial conserved population, the amplitude of genetic diversity decline in the context AS was more narrow extent than those in context AA, the ranges of decline of Ho and Ao were about 4 and 2 times in AA compared with that in AS, respectively, the occurrence of first monomorphic locus and the time of change of measure NP in scenario AA is 20 generations and 23 generations earlier than that in scenario AS, respectively. Additionally, we found that NRP, a novel measure proposed by our research group, was a proper estimate for monitoring the evolution of genetic diversity i

关键词： genetic diversity genetic architecture conservation simulation

A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO_2 thin film

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第11期10卷 860-866页

作者： Xiao-lin ZONG Chun-sheng WU Xiao-ling WU yun-feng lu Ping WANG Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Education Ministry Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China

Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS),fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that,with UV irradiation,the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.

关键词： DNA biosensor Titanium dioxide (Ti02) thin film Light addressable potentiometric sensor (LAPS) Silanization Fluorescein label Gene chip

Two-dimensional mesoporous materials:From fragile coatings to flexible membranes

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Chinese Chemical Letters 2013年第2期24卷 89-92页

作者： Zheng-Long Yang Jiao-Li Li Cheng-Liang Zhang yun-feng lu Zhen-Zhong Yang School of Chemistry and Materials Science Ludong University lnstitute of Chemistry Chinese Academy of Sciences Institute of Chemistry Chinese Academy of Sciences Department of Chemical and Biomolecular Engineering.University of California at Los Angeles Los AngelesCA 90095USA

Received 30 November 2012 Received in revised form 24 December 2012 Accepted 26 December 2012 Available online 29 January 2013

关键词： Mesoporous materialsTwo-dimensional materialsCoatingsMembranes

Porous copper cluster-based MOF with strong cuprophilic interactions for highly selective electrocatalytic reduction of CO_(2) to CH4

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Nano Research 2022年第12期15卷 10185-10193页

作者： Long-Zhang Dong yun-feng lu Rui Wang Jie Zhou Yu Zhang Lei Zhang Jiang Liu Shun-Li Li Ya-Qian Lan National and Local Joint Engineering Research Center of MPTES in High Energy and Safety LIBs Engineering Research Center of MTEES(Ministry of Education)Key Lab.of ETESPG(GHEI)School of ChemistrySouth China Normal UniversityGuangzhou510006China Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials Jiangsu Key Laboratory of New Power BatteriesSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing210023China

It is well known that the low-valent Cu species are important catalytically active centers in the reduction of CO_(2) to hydrocarbon ***,the Cu(l)-based catalysts are easily reduced during the electroreduction of CO_(2),which causes phase transformation of catalysts and leads to a decrease of intrinsic catalytic ***,it is of great significance to synthesize Cu(l)-based catalysts with specific interactions that can keep the catalytically active Cu sites stable in the electrocatalytic *** on the above considerations,a hexanuclear Cu cluster with strong cuprophilic interactions has been designed and utilized as a secondary building unit(SBU)to construct a stable metal-organic framework(MOF)electrocatalyst(NNU-50).As expected,the NNU-50 has served as an effective electrocatalyst for the CO_(2)-to-CH4 conversion by exhibiting a high Faradaic efficiency for CH4(FEcH4)of 66.40%and a large current density of~400 mA·cm-2 at-1.0 V *** hydrogen electrode(RHE),which is one of the best catalytic performances among the stable MOF electrocatalysts until *** work contributes more ideas for the design of stable and efficient MOF-based electrocatalysts for CO_(2) reduction reaction.

关键词： metal-organic framework cuprophilic interactions carbon dioxide electroreduction

Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds

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Journal of Integrative Agriculture 2017年第8期16卷 1789-1799页

作者： lu yun-feng CHEN Ji-bao ZHANG Bo LI Qing-gang WANG Zhi-xiu ZHANG Hao WU Ke-liang College of Animal Science and Technology China Agricultural University School of Life Science and Technology Nanyang Normal University Institute of Animal Sciences and Veterinary Medicine Anhui Academy of Agricultural Sciences

The enzyme △3,△2-dienoyl-CoA isomerase （ECI1） plays a crucial role in the mitochondria113-oxidation of fatty acids with a double-bond in odd and even positions. The ECll gene might be a qualified candidate for studies pertaining to lipid deposition and meat quality in swine. In the present study, ECI1 cDNA of the Tibetan pig was obtained by in silico cloning and verified by PCR analysis. Single-nucleotide polymorphisms （SNPs） of ECll were screened by PCR-sequencing and genotypes of those SNPs were tested by PCR-restriction fragment length polymorphism （PCR-RFLP） in Diannan small-ear pigs （DSP, n=40）, Tibetan pigs （TP, n=60） and Yorkshire pigs （YP, n=30）. The expression levels of ECll were analyzed by real-time quantitative PCR and Western blotting in tissues of the liver, backfat, and Iongissimus dorsi （LD） muscle of DSP （n=8）, TP （n=8） and YP （n=8）. Single factor linear correlation analysis was applied separately for each breed to evaluate correlations between ECll gene expression in the LD muscle and intramuscular fat （IMF） content. We obtained an ECll gene length of 1 401 bp from the cDNA that contained a full coding region of 909 bp. Three novel SNPs （g.42425337G〉A; g.42424666A〉G; and g.42422755A〉G） were detected, and only g.42424666A〉G exhibited three genotypes among the three breeds. The ECll expression levels in the LD muscle of DSP and TP were significantly higher than that of YP （P〈0.05）. Moreover, TP had the highest ECI1 expression in backfat （P〈O.01）, and a positive correlation was observed between gene expression and IMF content. The results suggest that differences in ECI1 gene expression might be related to lipid depo- sition and meat quality in pig.

关键词： ECll gene lipid deposition mRNA expression pig single-nucleotide polymorphism Western blotting

On-spot preparation of EDTA solution for the treatment of band keratopathy:a case report

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 767-769页

作者： Yan-Ting Li Bin Chen Tian-Hong Wu Bo Jiang yun-feng lu Department of Ophthalmology the First Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina Department of Ophthalmology Suzhou EENT HospitalSuzhou 215000Jiangsu ProvinceChina

Dear Editor,We report a case of band keratopathy who experienced several ocular surgeries,secondary uveitis and glaucoma which eventually led to severe band *** use commonly-used vacuum blood collection tubes with ethylenediaminetetraacetic acid dipotassium salt(EDTA-K2)to prepare chelation solution on-spot to remove the calcium deposits on the corneal *** is proved to be relatively safety,efficacy,cost-effective.

关键词： glaucoma eventually preparation

Diabetes mellitus in patients with type 1 autoimmune pancreatitis at diagnosis and after corticosteroid therapy

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Hepatobiliary & Pancreatic Diseases International 2024年第4期23卷 393-398页

作者： Mei-Zi Li Tao Guo yun-lu feng Sheng-Yu Zhang Xiao-Yin Bai Xi Wu Kai Xu Ai-Ming Yang Department of Gastroenterology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China Department of Radiology Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China

Background:A high prevalence of diabetes mellitus(DM)coexisting with autoimmune pancreatitis(AIP)is ***,evidence on the circumstances under which corticosteroid therapy(CST)for AIP improves or worsens DM is *** study aimed to demonstrate and identify predictors of DM control under the influence of ***:Patients diagnosed with type 1 AIP were enrolled from a prospectively maintained cohort and were classified into three groups according to the chronology in which AIP and DM were diagnosed:pre-existing DM(pDM),concurrent DM(cDM),and non-DM(nDM).The responses of DM to CST were assessed when corticosteroid was ceased or tapered to a maintenance dose and classified as‘improvement’and‘non-improvement’(including‘no change’and‘exacerbation’).Results:Among 101 patients with type 1 AIP,52(51.5%)patients were complicated with DM at the time of AIP diagnosis,with 36 patients in the cDM group and 16 patients in the pDM *** incidences of diffuse pancreatic swelling(72.2%)and pancreatic body/tail involvement(91.7%)were significantly higher in the cDM group than in both the pDM and nDM *** the 52 patients with DM,CST was administered in 48 *** logistic analysis identified that elevated serum gamma-glutamyl transferase(GGT)level at AIP diagnosis[odds ratio(OR)=0.032,95%confidence interval(CI):0.003-0.412,P=0.008]and pancreatic atrophy after CST(OR=0.027,95%CI:0.003-0.295,P=0.003)were negatively associated with DM control ***:Patients with diffuse pancreatic swelling and pancreatic body/tail involvement in pancreatitis tended to be complicated with cDM at AIP *** exerted a beneficial effect on the clinical course of DM in nearly half of the AIP patients complicated with DM at diagnosis,particularly in those without elevated serum GGT levels at diagnosis and who did not experience pancreatic atrophy after CST.

关键词： Type 1 autoimmune pancreatitis Diabetes mellitus Corticosteroid therapy Predictive factor Pancreatic atrophy

Short- and Long-term Therapeutic Efficacies of Intravenous Transplantation of Bone Marrow Stem Cells on Cardiac Function in Rats with Acute Myocardial Infarction:A Meta-analysis of Randomized Controlled Trials

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Chinese Medical Sciences Journal 2016年第3期31卷 142-148页

作者： Can Jiang Dong Zheng yun-lu feng Jun Guo Hai-rui Li Ai-dong Zhang Department of Cardiology the First Affiliated Hospital of Jinan University

Objective To investigate the short- and long-term therapeutic efficacies of intravenous transplantation of bone marrow stem cells(MSCs) in rats with experimental myocardial infarction by *** Randomized controlled trials were systematically searched from Pub Med,Science Citation Index(SCI),Chinese journal full-text database(CJFD) up to December *** the experimental groups(MSCs groups) were injected MSCs intravenously,the control groups were injected Delubecco's minimum essential medium(DMEM) or phosphate buffered saline(PBS).Subgroup analysis for each outcome measure was performed for the observing time point after the transplantation of *** mean differences(WMD) and 95% confidence intervals(CI) were calculated for outcome parameters including ejection fraction(EF) and fractional shortening(FS),which were measured by echocardiogram after intravenous injection and analyzed by Rev Man 5.2 and STATA *** Data from 9 studies(190 rats) were included in the *** compared to the control groups,the cardiac function of the experimental groups were not improved at day 7(EF:WMD=0.08,95%CI-1.32 to 1.16,P>0.01; FS:WMD=-0.12,95%CI-0.90 to 0.65,P>0.01) until at day 14 after MSCs' transplantation(EF:WMD=10.79,95%CI 9.16 to 12.42,Plusion The therapeutic efficacies of MSCs in rats with myocardid infarction become increasing apparent as time advances since 2 weeks after injection.

关键词： mesenchymal stem cells rats myocardial infarction intravenous

Pars plana vitrectomy with internal limiting membrane plugging assisted by autologous blood for optic disc pit associated with vitreomacular traction

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International Journal of Ophthalmology(English edition) 2024年第10期17卷 1957-1959页

作者： Bin Chen Yan-Ting Li Tian-Hong Wu Ya-Ping Yuan yun-feng lu Department of Ophthalmology Suzhou EENT HospitalSuzhou 215000Jiangsu ProvinceChina Department of Ophthalmology the First Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina

Dear Editor,We report a case of optic disc pit(ODP)associated with vitreomacular traction which has been successfully treated by a combined *** have obtained the written informed consent from the patient,and this case... 详细信息

关键词： technique traction macular