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Usefulness of the acromioclavicular joint cross-sectional area as a diagnostic image parameter of acromioclavicular osteoarthritis

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World Journal of Clinical Cases 2022年第7期10卷 2087-2094页

作者： Young Joo Jee Youn Moon Jung Youn Han yun-sic bang Keum Nae Kang Young Su Lim Young-SoonChoi Young-Uk Kim Department of Anesthesiology and Pain Medicine CHA Ilsan Medical CenterSchool of MedicineCHA UniversityIlsanGyeonggi-do 10414South Korea Department of Anesthesiology and Pain Medicine Seoul National University Hospital College of MedicineSeoul 03080South Korea Department of Anesthesiology and Pain Medicine CHA Bundang Medical CenterSchool of MedicineSeongnamGyeonggi-do 13496South Korea Department of Anesthesiology and Pain Medicine National Police HospitalSeoul 05715South Korea Department of Anesthesiology and Pain Medicine Catholic Kwandong UniversityCollege of MedicineInternational St.Mary’s HospitalIncheon 22711South Korea

BACKGROUND Acromioclavicular joint(ACJ)space narrowing has been considered to be an important diagnostic image parameter of ACJ osteoarthritis(ACJO).However,the morphology of the ACJ space is irregular because of osteophyte formation,subchondral irregularity,capsular distention,sclerosis,and ***,we created the ACJ cross-sectional area(ACJCSA)as a new diagnostic image parameter to assess the irregular morphologic changes of the *** To hypothesize that the ACJCSA is a new diagnostic image parameter for *** ACJ samples were obtained from 35 patients with ACJO and 30 healthy individuals who underwent shoulder magnetic resonance(S-MR)imaging that revealed no evidence of *** coronal,T2-weighted,fat-suppressed SMR images were acquired at the ACJ level from the two *** measured the ACJCSA and the ACJ space width(ACJSW)at the ACJ on the S-MR images using our imaging analysis *** ACJCSA was measured as the cross-sectional area of the *** ACJSW was measured as the narrowest point between the acromion and the *** The average ACJCSA was 39.88±10.60 mm;in the normal group and 18.80±5.13 mm;in the ACJO *** mean ACJSW was 3.51±0.58 mm in the normal group and 2.02±0.48 mm in the ACJO *** individuals had significantly lower ACJCSA and ACJSW than the healthy *** operating characteristic curve analyses demonstrated that the most suitable ACJCSA cutoff score was 26.14 mm^(2),with 91.4%sensitivity and 90.0%*** The optimal ACJSW cutoff score was 2.37 mm,with 88.6%sensitivity and 96.7%*** though both the ACJCSA and ACJSW were significantly associated with ACJO,the ACJCSA was a more sensitive diagnostic image parameter.

关键词： Acromioclavicular joint Osteoarthritis Cross-sectional area Diagnosis

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Low-molecular-weight fucoidan inhibits the proliferation of melanoma via Bcl-2 phosphorylation and PTEN/AKT pathway

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Oncology Research 2024年第2期32卷 273-282页

作者： MINJI PARK CHULHWAN bang WON-SOO yun yun-MI JEONG T&R Biofab Co. Ltd.Seongnam-si13487Korea Department of Dermatology Seoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul02706Korea Department of Mechanical Engineering Tech University of KoreaSi-heung City15073Korea

Fucoidan,a sulfate polysaccharide obtained from brown seaweed,has various bioactive properties,including anti-inflammatory,anti-cancer,anti-viral,anti-oxidant,anti-coagulant,anti-thrombotic,anti-angiogenic,and anti-Helicobacter pylori ***,the effects of low-molecular-weight fucoidan(LMW-F)on melanoma cell lines and three dimensional(3D)cell culture models are not well *** study aimed to investigate the effects of LMW-F on A375 human melanoma cells and cryopreserved biospecimens derived from patients with advanced *** wave was used to fragment fucoidan derived from Fucus vesiculosus into smaller *** and live/dead assays showed that LMW-F inhibited cell proliferation in both A375 cells and patientderived melanoma explants in a 3D-printed collagen *** PTEN/AKT pathway was found to be involved in the anti-melanoma effects of *** blot analysis revealed that LMW-F reduced the phosphorylation of Bcl-2 at Thr 56,which was associated with the prevention of anti-apoptotic activity of cancer *** findings suggested that LMW-F could enhance anti-melanoma chemotherapy and improve the outcomes of patients with melanoma resistance.

关键词： Low-molecular-weight fucoidan Melanoma Patient-derived melanoma explants in a 3D-printed collagen scaffold Anti-melanoma effect PTEN-AKT-Bcl-2 network

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High-resolution neutronics model for ^(238)Pu production in high-flux reactors

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Nuclear Science and Techniques 2024年第5期35卷 226-236页

作者： Qing-Quan Pan Qing-Fei Zhao Lian-Jie Wang bang-Yang Xia yun Cai Jin-Biao Xiong Xiao-Jing Liu School of Nuclear Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Science and Technology on Reactor System Design Technology Laboratory Nuclear Power Institute of ChinaChengdu 610200China

We proposed and compared three methods(filter burnup,single energy burnup,and burnup extremum analysis)to build a high-resolution neutronics model for 238Pu production in high-flux *** filter burnup and single energy burnup methods have no theoretical approximation and can achieve a spectrum resolution of up to~1 eV,thereby constructing the importance curve and yield curve of the full energy *** burnup extreme analysis method combines the importance and yield curves to consider the influence of irradiation time on production efficiency,thereby constructing extreme *** three curves,which quantify the transmutation rate of the nuclei in each energy region,are of physical significance because they have similar distributions.A high-resolution neutronics model for ^(238)Pu production was established based on these three curves,and its universality and feasibility were *** neutronics model can guide the neutron spectrum optimization and improve the yield of ^(238)Pu by up to 18.81%.The neutronics model revealed the law of nuclei transmutation in all energy regions with high spectrum resolution,thus providing theoretical support for high-flux reactor design and irradiation production of ^(238)Pu.

关键词： ^(238)Pu Neutronics model High-flux reactor Spectrum resolution Spectrum optimization

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SARS-CoV-2 envelope protein impairs airway epithelial barrier function and exacerbates airway inflammation via increased intracellular Cl^(-)concentration

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1745-1759页

作者： Jian-bang Xu Wei-Jie Guan Yi-Lin Zhang Zhuo-Er Qiu Lei Chen Xiao-Chun Hou Junqing Yue Yu-yun Zhou Jie Sheng Lei Zhao yun-Xin Zhu Jing Sun Jincun Zhao Wen-Liang Zhou Nan-Shan Zhong State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseNational Center for Respiratory MedicineDepartment of Respiratory and Critical Care MedicineGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouP.R.China Department of Thoracic Surgery Guangzhou Institute for Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouP.R.China Guangzhou National Laboratory GuangzhouP.R.China School of Life Sciences Sun Yat-sen UniversityGuangzhouP.R.China Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes School of Life SciencesSun Yat-sen UniversityGuangzhouP.R.China Department of Physiology School of Basic Medical SciencesGuangzhou Medical UniversityGuangzhouP.R.China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection disrupts the epithelial barrier and triggers airway *** envelope(E)protein,a core virulence structural component of coronaviruses,may play a role in this *** could interfere with transepithelial Cl^(-)transport via impairment of the cystic fibrosis transmembrane conductance regulator(CFTR),which modulates nuclear factor kB(NF-kB)***,the pathological effects of SARS-CoV-2 E protein on airway epithelial barrier function,Cl^(-)transport and the robust inflammatory response remain to be ***,we have demonstrated that E protein down-regulated the expression of tight junctional proteins,leading to the disruption of the airway epithelial *** addition,E protein triggered the activation of Toll-like receptor(TLR)2/4 and downstream c-Jun N-terminal kinase(JNK)signaling,resulting in an increased intracellular Cl^(-)concentration([Cl^(-)]_(i))via up-regulating phosphodiesterase 4D(PDE4D)expression in airway epithelial *** elevated[Cl^(-)]_(i);contributed to the heightened airway inflammation through promoting the phosphorylation of serum/glucocorticoid regulated kinase 1(SGK1).Moreover,blockade of SGK1 or PDE4 alleviated the robust inflammatory response induced by E ***,these findings provide novel insights into the pathogenic role of SARS-CoV-2 E protein in airway epithelial damage and the ongoing airway inflammation during SARS-CoV-2 infection.

关键词： airway inflammation elevated

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Electroacupuncture Promotes Angiogenesis in Mice with Cerebral Ischemia by Inhibiting miR-7

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Chinese Journal of Integrative Medicine 2024年第6期30卷 543-550页

作者： YU Qian SHU Shi JU Xin-yao PENG Wei REN Xue-qi SI Shu-han SONG Shi-zhen XIE Xue-yun FANG bang-jiang ZHOU Shuang School of Acupuncture-Moxibustion and Tuina Shanghai University of Traditional Chinese MedicineShanghai201203China College of Basic Medicine Shanghai University of Traditional Chinese MedicineShanghai201203China Department of Emergency Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai200032China

Objective To observe the angiogenesis effect of electroacupuncture(EA)at Shuigou acupoint(GV 26)in the treatment of cerebral ischemia,and explore the value of miRNA-7(miR-7)in *** First,48 mice were randomly divided into sham operation,middle cerebral artery occlusion(MCAO)model,and EA treatment *** 9 mice were divided into carrier control group,miR-7 knockout group and miR-7 overexpression group(n=3 each group).Finally,20 mice were divided into model and carrier control group,model and miR-7 knockout group,EA treatment and carrier control group and EA treatment and miR-7 overexpression group,with 3–6 mice in each *** MCAO model was established in the MCAO and EA *** deficit score and 2,3,5-triphenyltetrazolium chloride(TTC)staining were used to evaluate the severity of cerebral ***-eosin staining was used to describe basic pathological *** was used to quantify cerebral microvessel ***-time PCR and Western blot were used to detect the expression of miR-7 and its downstream target genes Krüppel-like factor 4/vascular endothelial growth factor(KLF4/VEGF)and angiopoietin-2(ANG-2)in the ischemic cerebral *** After EA,neurological deficit scores and infarction volumes decreased,and the density of cerebral microvessels *** the MCAO group,miR-7 expression was higher than that in the sham group(P<0.01).After EA at GV 26,miR-7 expression decreased(P<0.01)and the expression of downstream target genes KLF4/VEGF and ANG-2 increased as compared with the MCAO group(P<0.01).After EA combined with overexpression of miR-7,the expression of downstream target genes KLF4/VEGF and ANG-2 decreased compared to the control EA group(P<0.01).After miR-7 knockdown,the expression of KLF4/VEGF and ANG-2 increased(P<0.05 or P<0.01).Conclusions EA could promote angiogenesis in MCAO mice likely by inhibiting the expression of miR-7 and relieving inhibition of downstream target genes KLF4/VEG

关键词： electroacupuncture cerebral ischemia miRNA-7 angiogenesis Shuigou(GV 26) acupuncture points

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马克思恩格斯共同体理论对增进中华民族共同性的三个启示

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中南民族大学学报（人文社会科学版） 2024年第2期44卷 10-16,181页

作者：杜帮云熊坤新云南大学马克思主义学院云南昆明650091 中央民族大学中国民族理论与民族政策研究院北京100081

共同体理论是马克思恩格斯关于人类社会发展理论的重要基石。马克思恩格斯认为人是共同体中的存在,推崇“真正的共同体”;认为人们在共同体中共存互补,注重交往与联合;认为共同利益是把共同体成员维系在一起的纽带,强调成员利益的共同... 详细信息

共同体理论是马克思恩格斯关于人类社会发展理论的重要基石。马克思恩格斯认为人是共同体中的存在,推崇“真正的共同体”;认为人们在共同体中共存互补,注重交往与联合;认为共同利益是把共同体成员维系在一起的纽带,强调成员利益的共同性与一致性。马克思恩格斯共同体理论既是理解中华民族共同体的钥匙,也是铸牢中华民族共同体意识的重要学理支撑,对增进中华民族共同性有三方面启示:有助于深刻认识中华民族共同体的形成、演进与时代价值,增强对它的认同,在对“真正的共同体”的追求中,坚定对中国特色社会主义的信念和对共产主义的信仰;有助于团结共同主体,充分发挥各族人民的主动性、积极性和创造性;有助于促进共同利益认同,实现好、维护好、发展好各族人民的根本利益。

关键词：马克思恩格斯共同体理论中华民族共同性共同意识共同主体共同利益

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Thermodynamic equilibrium theory-guided design and synthesis of Mg-doped LiFe_(0.4)Mn_(0.6)PO_(4)/C cathode for lithium-ion batteries

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Journal of Energy Chemistry 2024年第4期91卷 619-627页

作者： Wei Lyu Wenlong Cai Tuan Wang Xiaobo Sun Enhao Xu Jinxuan Chen Kaipeng Wu yun Zhang College of Materials Science and Engineering Sichuan UniversityChengdu 610064SichuanChina

Mn-rich LiFe_(1-x)Mn_(x)PO_(4)(x>0.5),which combines the high operation voltage of LiMnPO_(4)with excellent rate performa nce of LiFePO4,is hindered by its sluggish kinetic ***,thermodynamic equilibrium analysis of Mn^(2+)-Fe^(2+)-Mg^(2+)-C_(2)O_(4)^(2-)-H_(2)O system is used to guide the design and preparation of insitu Mg-doped(Fe_(0.4)Mn_(0.6))_(1-x)Mg_(x)C_(2)O_(4)intermediate,which is then employed as an innovative precursor to synthesize high-performance Mg-doped LiFe_(0.4)Mn_(0.6)PO_(4).It indicates that the metal ions with a high precipitation efficiency and the stoichiometric precursors with uniform element distribution can be achieved under the optimized thermodynamic ***,accelerated Li+diffusivity and reduced charge transfer resistance originating from Mg doping are verified by various kinetic *** from the contributions of inherited homogeneous element distribution,small particle size,uniform carbon layer coating,enhanced Li+migration ability and structural stability induced by Mg doping,the Li(Fe_(0.4)Mn_(0.6))_(0.97)Mg_(0.03)PO_(4)/C exhibits splendid electrochemical performance.

关键词： (Fe_(0.4)Mn_(0.6))_(1-x)MgxC_(2)0_(4) precursors Thermodynami cequilibrium Co-precipitation Mg doping Lithium-ion batteries

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Boosting rate performance of layered lithium-rich cathode materials by oxygen vacancy induced surface multicomponent integration

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Journal of Energy Chemistry 2024年第5期92卷 250-262页

作者： Youyou Fang Yuefeng Su Jinyang Dong Jiayu Zhao Haoyu Wang yun Lu Bin Zhang Hua Yan Feng Wu Lai Chen Beijing Key Laboratory of Environmental Science and Engineering School of Material Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Chongqing Innovation Center Beijing Institute of TechnologyChongqing 401120China Yibin Libode New Materials Co. Ltd.Yibin 64400SichuanChina School of Metallurgy and Environment Central South UniversityChangsha 410083HunanChina Engineering Research Center of the Ministry of Education for Advanced Battery Materials Central South UniversityChangsha 410083HunanChina

The rapid development of electric vehicles and portable energy storage systems demands improvements in the energy density and cost-effectiveness of lithium-ion batteries,a domain in which Lithium-rich layered cathode(LLO)materials inherently ***,these materials face practical challenges,such as low initial Coulombic efficiency,inferior cycle/rate performance,and voltage decline during cycling,which limit practical *** study introduces a surface multi-component integration strategy that incorporates oxygen vacancies into the pristine LLO material Li1.2Mn_(0.6)Ni_(0.2)O_(2).This process involves a brief citric acid treatment followed by calcination,aiming to explore rate-dependent degradation *** induced surface oxygen vacancies can reduce surface oxygen partial pressure and diminish the generation of O_(2)and other highly reactive oxygen species on the surface,thereby facilitating the activation of Li ions trapped in tetrahedral sites while overcoming transport ***,the formation of a spinel-like phase with 3D Li+diffusion channels significantly improves Li^(+)diffusion kinetics and stabilizes the surface *** optimally modified sample boasts a discharge capacity of 299.5 mA h g^(-1)at a 0.1 C and 251.6 mA h g^(-1)at a 1 C during the initial activation cycle,with an impressive capacity of 222.1 mA h g^(-1)at a 5 *** notably,it retained nearly 70%of its capacity after 300 cycles at this elevated *** straightforward,effective,and highly viable modification strategy provides a crucial resolution for overcoming challenges associated with LLO materials,making them more suitable for practical application.

关键词： Lithium-ion battery Layered lithium rich cathode Surface multicomponent integration Rate-dependent degradation and Li^(+) diffusion kinetics

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薯蓣皂苷通过调控AMPK/mTOR自噬信号通路影响骨肉瘤细胞增殖及侵袭的机制研究

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浙江中西医结合杂志 2024年第4期34卷 304-309,316页

作者：吴良邦葛云林中国人民解放军联勤保障部队903医院(杭州医学院附属西湖医院)骨三科杭州311243

目的探讨薯蓣皂苷(Dioscin)通过调控腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)自噬信号通路对人骨肉瘤MG63细胞增殖及侵袭的影响。方法取对数期生长人骨肉瘤MG63细胞分为空白对照组、Dioscin不同剂量组(20、40、80、100μ... 详细信息

目的探讨薯蓣皂苷(Dioscin)通过调控腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)自噬信号通路对人骨肉瘤MG63细胞增殖及侵袭的影响。方法取对数期生长人骨肉瘤MG63细胞分为空白对照组、Dioscin不同剂量组(20、40、80、100μmol/L)。CCK-8法检测细胞增殖能力,划痕实验检测细胞迁移能力,细胞迁移实验检测细胞侵袭能力,丹酰尸胺法观察细胞自噬情况,蛋白质印迹法检测磷酸化腺苷酸活化蛋白激酶(p-AMPK)/AMPK、磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)/mTOR、转录因子EB(TFEB)、人微管相关蛋白轻链3Ⅱ(LC3-Ⅱ)、自噬效应蛋白beclin-1表达水平。结果与空白对照组比较,Dioscin20、40、80、100μmol/L组MG63细胞增殖率[(65.21±4.09)%、(51.94±3.95)%、(46.57±3.72)%、(37.31±4.23)%比100.00%]、划痕愈合率[(85.43±3.84)%、(70.62±3.17)%、(55.83±2.92)%、(44.23±3.27)%比(98.25±1.23)%]、侵袭细胞比率[(72.43±2.83)%、(63.76±3.34)%、(51.92±3.13)%、(42.44±3.09)%比(91.14±2.91)%]依次降低,呈浓度依赖性(P<0.05);空白对照组中仅有少量细胞自噬泡,作用48 h后Dioscin各组自噬泡依次增多;与空白对照组比较,Dioscin20、40、80、100μmol/L组MG63细胞中p-AMPK/AMPK[(0.35±0.04)、(0.50±0.02)、(0.60±0.03)、(0.88±0.03)比(0.20±0.02)]、TFEB[(0.42±0.02)、(0.68±0.03)、(0.92±0.04)、(1.36±0.10)比(0.30±0.02)]、LC3-Ⅱ[(0.41±0.03)、(0.70±0.04)、(0.88±0.03)、(1.17±0.07)比(0.26±0.02)]、beclin-1[(0.32±0.03)、(0.50±0.04)、(0.68±0.05)、(0.75±0.06)比(0.16±0.02)]蛋白表达依次增加,p-mTOR/mTOR[(0.75±0.02)、(0.52±0.03)、(0.40±0.04)、(0.31±0.02)比(0.86±0.05)]蛋白表达依次降低,差异均具有统计学意义(P<0.05);与Dioscin(100μmol/L)组比较,Dioscin(100μmol/L)+AMPK抑制剂(Compound C 10μmol/L)组p-AMPK/AMPK[(0.49±0.03)比(0.63±0.04)]、TFEB[(0.16±0.03)比(0.24±0.02)]、LC3-Ⅱ[(0.35±0.02)比(0.44±0.04)]、beclin-1[(0.44±0.03)比(0.56±0.04)]蛋白表达水平明显降低,p-mTOR/mTOR[(0.63±0.05)比(0.45±0.04)]表达明显升高(P<0.05)。结论Dioscin可能通过调控AMPK/mTOR-TFEB通路抑制骨肉瘤细胞增殖、迁移及侵袭。

关键词：骨肉瘤薯蓣皂苷自噬 AMPK/mTOR信号通路

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植物促生菌暹罗芽孢杆菌37402-1的功能特性及对大蒜的促生效果

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中国土壤与肥料 2024年第2期 185-192页

作者：张亚楠王凯望牛邦彦王丽张梦琦陈云云王纪忠高淼淮阴工学院生命科学与食品工程学院江苏淮安223003 北方干旱半干旱耕地高效利用重点实验室中国农业科学院农业资源与农业区划研究所北京100081 西安种子管理站陕西西安710054 北京农业职业学院食品与生物工程学院北京102442

大蒜(Allium sativum L.)是我国的重要经济作物,主产地连作障碍严重,其种植生产的持续性受到制约。植物根际促生菌(PGPR)能够促进植物生长、防治病害且对环境友好。研制高效防控大蒜土传病害的微生物肥料对我国大蒜产业可持续发展和绿... 详细信息

大蒜(Allium sativum L.)是我国的重要经济作物,主产地连作障碍严重,其种植生产的持续性受到制约。植物根际促生菌(PGPR)能够促进植物生长、防治病害且对环境友好。研制高效防控大蒜土传病害的微生物肥料对我国大蒜产业可持续发展和绿色发展有着重要的生态学和现实意义。以实验室前期从山东省金乡县连作大蒜根部分离的细菌为供试菌株,筛选获得对大蒜根腐病病原真菌尖刀镰孢菌(Fusarium oxysporum)ACCC37402和拟枝孢镰刀菌(Fusarium sporotrichioides)ACCC36464具有较好拮抗效果的菌株37402-1。菌株37402-1的PGPR功能特性研究结果显示:对大蒜根腐病病原真菌ACCC37402和ACCC36464的抑菌率分别为52.70%和37.98%;经16S rDNA基因序列同源性分析,确定为暹罗芽孢杆菌(Bacillus siamensis);具有水解蛋白能力、产铁载体能力、溶解有机磷和无机磷的能力。温室盆栽试验显示,接种暹罗芽孢杆菌37402-1菌悬液能够促进大蒜幼苗的生长。田间试验表明,接种暹罗芽孢杆菌37402-1菌剂使大蒜增产12.78%,并改变土壤酶活性(提高土壤蔗糖酶和土壤脲酶活性,降低土壤过氧化氢酶活性)。菌株暹罗芽孢杆菌37402-1兼具多种功能特性,对大蒜根腐病具有较好的拮抗效果,同时可以显著促进大蒜幼苗生长,并可在大田条件下促进大蒜的单产,有望开发成为高效的微生物肥料生产菌种。

关键词：大蒜植物根际促生菌暹罗芽孢杆菌促生抗病

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