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番石榴抗坏血酸合成酶基因在果实发育过程中表达特性分析

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南方农业学报 2024年第2期55卷 422-431页

作者：萧允艺赵晓梦刘金丰于泽浩刘杰凤林丽静广东石油化工学院生物与食品工程学院广东茂名525000 中国热带农业科学院农产品加工研究所/海南省果蔬贮藏与加工重点实验室广东湛江524001

【目的】测定番石榴果实发育过程中的抗坏血酸含量,并分析番石榴抗坏血酸合成酶基因的表达特性,为后续深入挖掘果实抗坏血酸合成调控机理及选育优质番石榴品种提供理论参考。【方法】以番石榴品种珍珠果实为研究对象,测定不同果实发育(... 详细信息

【目的】测定番石榴果实发育过程中的抗坏血酸含量,并分析番石榴抗坏血酸合成酶基因的表达特性,为后续深入挖掘果实抗坏血酸合成调控机理及选育优质番石榴品种提供理论参考。【方法】以番石榴品种珍珠果实为研究对象,测定不同果实发育(花后10~130 d)过程中果肉(鲜重)中抗坏血酸含量及单果抗坏血酸总量,并从番石榴基因组数据中挖掘其抗坏血酸合成途径酶基因,通过实时荧光定量PCR检测其在果实发育过程中的表达特性。【结果】珍珠番石榴品种果实发育成熟周期为130 d,生长速率出现2个高峰期,分别出现在幼果膨大期和大果膨大期,果实果皮颜色从深绿色向浅绿转变,在花后110 d果皮颜色转变最明显。番石榴果肉抗坏血酸含量在果实发育成熟前期(花后10~40d)保持在较高水平,特别是在花后30d,果肉抗坏血酸含量达132mg/100g,在花后40~100d抗坏血酸含量稍微下降但较稳定,保持在102.73 mg/100 g以上,成熟后期随果实迅速膨大而抗坏血酸含量略有下降,为84.39 mg/100 g,但单果抗坏血酸总量持续上升。从番石榴基因组数据共鉴定获得13个基因编码果实抗坏血酸合成途径相关酶的基因,这些基因编码完整l-半乳糖途径的相关酶,其中有6个基因集中于3号染色体,推测3号染色体是番石榴果实维生素合成的关键染色体。实时荧光定量PCR检测结果显示,除磷酸甘露糖异构酶基因(PgPMI1)和甘露糖磷酸变位酶基因(PgPMM1)在果实发育成熟过程中表达不规律外,其余11个l-半乳糖途径相关酶基因均在果实发育中期上调表达,后期表达又下调。果实抗坏血酸含量与GDP-甘露糖焦磷酸化酶基因(PgGMP1)和GDP-甘露糖-3',5'-表型异构酶基因(gGME2)表达显著正相关(Pl-半乳糖途径存在复杂的分子调控网络,挖掘获得的13个l-半乳糖途径成员,可用于后续番石榴优良品种的选育及转录调控机理研究。

关键词：番石榴抗坏血酸果实发育基因表达

超高效液相色谱-串联质谱法测定饮料中高氯酸盐和氯酸盐

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卫生研究 2024年第1期53卷 102-108页

作者：陈东李倩刘平陈忠辉谢昀许秀丽张晶张峰北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室北京100013 北京市大兴区疾病预防控制中心北京102600 中国检验检疫科学研究院北京100176

目的建立同位素内标法定量测定饮料中高氯酸盐和氯酸盐的高效液相色谱-串联质谱法。方法液体饮料样品经甲醇溶液提取,固体饮料样品经乙酸水提取,PSA/C18基质分散固相萃取净化,再经Acquity CSH氟苯基色谱柱(100 mm×2.1 mm,1.7μm)分离,... 详细信息

目的建立同位素内标法定量测定饮料中高氯酸盐和氯酸盐的高效液相色谱-串联质谱法。方法液体饮料样品经甲醇溶液提取,固体饮料样品经乙酸水提取,PSA/C18基质分散固相萃取净化,再经Acquity CSH氟苯基色谱柱(100 mm×2.1 mm,1.7μm)分离,串联质谱法检测,内标法定量。结果高氯酸盐和氯酸盐在各自的线性范围内峰面积比与其质量浓度线性关系良好,相关系数(r)大于0.999;高氯酸盐和氯酸盐的检出限分别为0.2和1μg/l,定量限为0.5和3μg/l;两种化合物的平均回收率分别为84.0%~105.0%和82.7%~112.1%,相对标准偏差为4.2%~17.0%和5.5%~18.4%(n=6)。11类饮料样品中高氯酸盐含量为0.53~4.12μg/l,氯酸盐含量为3.27~61.86μg/l。结论本方法前处理简单、灵敏度高、准确、可靠,适用于饮料中高氯酸盐和氯酸盐的测定。

关键词：高氯酸盐氯酸盐同位素内标法饮料超高效液相色谱-串联质谱法

固废基气凝胶粒子电极的制备及电催化降解有机污染物的性能与机制

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高等学校化学学报 2024年第10期45卷 57-68页

作者：李赟李红艳张峰肖子君王芳崔佳丽杨群太原理工大学环境科学与工程学院山西省市政工程研究生教育创新中心晋中030600 吕梁学院建筑与土木工程系吕梁033001 哈尔滨工业大学环境学院城市水资源与水环境国家重点实验室哈尔滨150090 山西省生物研究院有限公司食用菌研究中心太原030006 黄土高原食用菌山西省重点实验室太谷030801

以废菌棒(MS)和赤泥(RM)为原料,经过煅烧得到粉末状废菌棒赤泥生物炭(MSRMBC).采用悬浮滴定CaCl_(2)法以海藻酸钠(SA)为骨架的凝胶对MSRMBC进行包埋制得气凝胶粒子电极(MSRMBC@SA),并利用扫描电子显微镜(SEM)、能量色散谱(EDS)、傅里叶... 详细信息

以废菌棒(MS)和赤泥(RM)为原料,经过煅烧得到粉末状废菌棒赤泥生物炭(MSRMBC).采用悬浮滴定CaCl_(2)法以海藻酸钠(SA)为骨架的凝胶对MSRMBC进行包埋制得气凝胶粒子电极(MSRMBC@SA),并利用扫描电子显微镜(SEM)、能量色散谱(EDS)、傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)、氮气吸附-脱附实验和循环伏安曲线(CV)对其进行表征分析.利用MSRMBC@SA构建三维电芬顿体系(3D-EFMSRMBC@SA),以卡马西平(CBZ)为模型化合物,考察了MSRMBC@SA投加量、电压、曝气量和pH值对MSRMBC@SA电催化性能的影响,最佳降解条件下CBZ(10 mg/l)的降解率为86.05%.因MSRMBC@SA具有良好的pH值调节能力,故体系受pH值影响较小.自由基猝灭、电子自旋共振(ESR)及X射线光电子能谱(XPS)表征结果表明,·OH为主要活性物种,MSRMBC@SA在CBZ降解过程中起到了关键的催化作用.研究发现,MSRMBC@SA稳定性较好,且3D-EF-MSRMBC@SA较2D-EF及3D-EF-MSRMBC具有更低的能耗和更佳的降解效果.MSRMBC@SA可有效降低CBZ溶液的生物毒性,对实际CBZ抗生素废水降解具有良好的应用前景.

关键词：气凝胶粒子电极高级氧化技术卡马西平三维电芬顿体系降解机理

Immunoglobulin G4-related lymph node disease with an orbital mass mimicking Castleman disease:A case report

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World Journal of Clinical Cases 2021年第35期9卷 10999-11006页

作者： feng-yun Hao feng-Xia Yang Hai-Yan Bian Xia Zhao Department of Pathology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Abdominal Ultrasound The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Hematology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Immunoglobulin(Ig)G4-associated diseases are a group of systemic diseases involving multiple organs and are also known as IgG4-associated sclerosing ***4-associated lymphadenopathy occurring in the lymph nodes is characterized by a lack of specificity due to its clinicopathological characteristics and must be differentiated from a variety of lesions,such as Castleman disease,lymphatic follicular reactive hyperplasia,and *** SUMMARY A 65-year-old male patient,with Guillain-Barre syndrome for 5 years,presented to our hospital complaining of bilateral orbital mass for 2 *** hospitalization,the results of the patient’s laboratory tests showed that immunoglobulin subgroup IgG4 was 33.90 g/l and IgG was 30.30 g/l,but serum interleukin-6 was *** pathological morphology of orbital mass and cervical lymph node were consistent,which showed that a large number of plasma cells and eosinophils were observed in the lymphatic follicles,and the interstitial fibrous tissue was *** showed that CD20(B cells)(+),CD3(T cells)(+),CD38(+),IgG(+),IgG4 positive cells>100/high powered field,and IgG4/IgG>40%.Combined with clinical and immunohistochemical results,lymphadenopathy was consistent with Castleman disease-like IgG4-associated sclerosing *** acetate treatment was given at 40 mg/*** 2 wk,the superficial lymph nodes and orbital masses shrank,and the IgG4 level *** prednisone acetate was regularly used at a reduced dosage,no recurrence of the disease has been *** This case suggested that it is necessary to proceed cautiously in clinical practice with such patients,and immunoglobulin,complement,interleukin-6,C-reactive protein,and other examinations should be performed to confirm the diagnosis.

关键词： IgG4-associated disease Castleman disease lymphadenopathy Orbital neoplasm Pathological morphology Case report

两种沉水植物及其叶面附着细菌对钙磷添加的响应研究

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水生态学杂志 2024年第3期45卷 103-111页

作者：王和云胡睿周易陈矿程华强常锋毅湖北工业大学土木建筑与环境学院河湖生态修复及藻类利用湖北省重点实验室湖北武汉430068 武汉市市政建设集团有限公司湖北武汉430023

沉水植物光合作用形成的微环境有利于水体中钙和磷形成CaCO_(3)-P共沉淀,但不同的沉水植物种类诱导CaCO_(3)-P共沉淀的能力不同。本研究以菹草和伊乐藻为研究对象,设置钙离子添加质量浓度(0和100 mg/l)和无机磷添加质量浓度(0、0.2和2 m... 详细信息

沉水植物光合作用形成的微环境有利于水体中钙和磷形成CaCO_(3)-P共沉淀,但不同的沉水植物种类诱导CaCO_(3)-P共沉淀的能力不同。本研究以菹草和伊乐藻为研究对象,设置钙离子添加质量浓度(0和100 mg/l)和无机磷添加质量浓度(0、0.2和2 mg/l)2个变量,通过测定培养液pH,总磷(TP)、溶解性活性磷(SRP)、叶绿素含量(Chl-a)和碱性磷酸酶活性(TAPA),植株干重全磷、灰分总磷和钙磷的含量及叶面附着细菌的群落特征,探讨植物及其叶面附着细菌对水体钙磷添加的响应。研究结果表明:(1)钙离子添加降低了水体TP和SRP,水体TAPA与pH负相关,菹草体系中,Chl-a与水体pH负相关,而伊乐藻体系中,Chl-a与pH正相关,添加100 mg/l钙离子浓度处理后,菹草上覆水中TAPA与SRP、TP负相关,而伊乐藻上覆水Chl-a与TP、SRP的正向关联被干扰;(2)菹草与伊乐藻的灰分总磷和钙磷的含量随磷浓度升高而增加,添加100 mg/l钙离子浓度处理后,菹草的灰分总磷和钙磷的含量升高,而伊乐藻的灰分总磷和钙磷含量降低,菹草的灰分钙磷含量大于伊乐藻;(3)菹草和伊乐藻叶面附着细菌中,变形菌门(Proteobacteria)与蓝藻门(Cyanobacteria)的相对丰度均位于前两名,两者之和占相对丰度的71%~84%,无钙离子添加处理中,添加2 mg/l磷处理中,菹草和伊乐藻叶面附着变形菌门丰度降低,蓝藻门丰度升高,添加100 mg/l钙离子后,随磷浓度升高,菹草变形菌门丰度升高,蓝藻门丰度降低,而伊乐藻变形菌门丰度变化不大,但蓝藻门丰度升高。总之,由于钙磷共沉淀能力的差异,2种植物的钙磷含量、植物叶面的形态结构和细菌群落组成表现出明显的种间差异。

关键词：沉水植物叶面附着细菌群落灰分磷钙磷

Homocysteine is associated with the progression of non-culprit coronary lesions in elderly acute coronary syndrome patients after percutaneous coronary intervention

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Journal of Geriatric Cardiology 2016年第4期13卷 299-305页

作者： Tian-Wen HAN Shan-Shan ZHOU Jian-Tao lI feng TIAN Yang MU Jing JING yun-feng HAN yun-Dai CHEN Department of Cardiology Chinese PLA General Hospital Beijing China

Background The influence of homocysteine （Hcy） on the migration and proliferation of vascular smooth muscle cells has been well established. However, the impact of Hcy levels on the progression of non-culprit coronary lesions （NCCls） is controversial. This study aims to evaluate whether the plasma level of Hcy is related to the progression of NCCls after percutaneous coronary stent implantation in elderly patients with acute coronary syndrome （ACS）. Methods A total of 223 elderly patients （〉 65 years old） with ACS undergoing stent im- plantation and follow-up coronary angiography were enrolled. laboratory determination comprised of blood sample evaluation for Hcy was carried out before baseline coronary intervention. The patients were classified into two groups according to the blood Hcy tertiles （〉 15 mmol/l or 〈 15 mmol/l）. Patients were followed up for 12.2 months. NCCl progression was assessed by three-dimensional quantitative coronary angiography. Results A significantly higher ratio of NCCl progression was observed in the group with baseline Hcy concentrations above 15 mmol/l compared to the group with concentrations below 15 mmol/l （41/127, 32.3% vs. 14/96, 14.6%, P = 0.002）. Multivariate Cox regression analysis showed that Hcy and diabetes mellitus were independent risk factors for NCCl progression. The crude haz- ard ratio （HR） of NCCl progression for Hcy level was 1.056 （95% CI： 1.01-1.104, P = 0.015）. The adjusted HR of NCCl progression for Hcy level was 1.024 （95% CI： 1.007-1.042, P = 0.007）. The adjusted HR of NCCl progression for diabetes mellitus was 1.992 （95% CI： 1.15-3.44, P = 0.013）. Conclusions Hcy is an independent risk factor for NCCl progression after 12 months of follow-up in elderly patients with ACS who has undergone percutaneous coronary stenting.

关键词： Coronary angiography Elderly patients Homocysteine Non-culprit coronary lesion Percutaneous coronary intervention

低蛋白氨基酸平衡饲粮对福建龙岩蛋鸭生产性能、蛋品质、血清指标和氮排放的影响

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饲料研究 2024年第3期47卷 47-52页

作者：许云英丁梓钊肖长峰吕文纬潘明官王惠影朱丽慧上海农好饲料股份有限公司上海201501 上海市农业科学院畜牧兽医研究所上海201106

试验旨在研究低蛋白氨基酸平衡饲粮对产蛋高峰期龙岩蛋鸭生产性能、蛋品质、血清指标及粪氮含量的影响。选用540只140日龄健康蛋鸭,随机分为3组。对照组饲喂粗蛋白水平为17.31%的饲粮,试验1组和试验2组分别饲喂16.20%和15.30%粗蛋白平... 详细信息

试验旨在研究低蛋白氨基酸平衡饲粮对产蛋高峰期龙岩蛋鸭生产性能、蛋品质、血清指标及粪氮含量的影响。选用540只140日龄健康蛋鸭,随机分为3组。对照组饲喂粗蛋白水平为17.31%的饲粮,试验1组和试验2组分别饲喂16.20%和15.30%粗蛋白平衡氨基酸的饲粮。正式试验期为98 d。结果显示:与对照组和试验2组相比,试验1组的蛋黄重和蛋重显著提高(P<0.05);试验2组血清超氧化物歧化酶(SOD)活性显著高于其他两组(P<0.05),尿素氮(BUN)含量显著高于试验1组(P<0.05)。试验2组和对照组的干物质和粗纤维表观消化率显著高于试验1组(P<0.05)。随着粗蛋白水平的降低,粗蛋白表观消化率显著升高(P<0.05),粪氮含量显著降低(P<0.05)。研究表明,产蛋高峰期蛋鸭饲粮粗蛋白水平为17.31%时降低1.0%~2.5%的粗蛋白水平,并平衡必需氨基酸,对其生产性能、蛋品质以及血清生化和抗氧化指标无负面影响,并且可以降低粪氮含量。

关键词：龙岩蛋鸭低蛋白氨基酸平衡饲粮蛋品质抗氧化氮排放

Blue light is more essential than red light for maintaining the activities of photosystem Ⅱ and Ⅰ and photosynthetic electron transport capacity in cucumber leaves

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Journal of Integrative Agriculture 2016年第1期15卷 87-100页

作者： MIAO Yan-xiu W ang Xiao-zhuo G AO li-hong C HEN Qing-yun QU Mei Beijing Key laboratory of Growth and Developmental Regulation for Protected Vegetable Crops College of Agriculture and Biotechnology China Agricultural UniversityBeijing 100193P.R.China

Blue and red lights differently regulate leaf photosynthesis. Previous studies indicated that plants under blue light generally exhibit better photosynthetic characteristics than those under red light. However, the regulation mechanism of related photosynthesis characteristics remains largely unclear. Here, four light qualities treatments （300 μmol m-2 s-1） including white fluorescent light （Fl）, blue monochromatic light （B, 440 nm）, red monochromatic light （R, 660 nm）, and a combination of red and blue light （RB, R：B=8：1） were carried out to investigate their effects on the activity of photosystem II （PSII） and photosystem I （PSI）, and photosynthetic electron transport capacity in the leaves of cucumber （Cucumis sativus l.） seedlings. The results showed that compared to the Fl treatment, the R treatment significantly limited electron transport rate in PSII （ETR11） and in PSI （ETR1） by 79.4 and 66.3%, respectively, increased non-light induced non-photochemical quenching in PSII （q^No） and limitation of donor side in PSI （φND） and reduced most JIP-test parameters, suggesting that the R treatment induced suboptimal activity of photosystems and inhibited electron transport from PSII donor side up to PSI. However, these suppressions were effectively alleviated by blue light addition （RB）. Compared with the R treatment, the RB treatment significantly increased ETR, and ETR1 by 176.9 and 127.0%, respectively, promoted photosystems activity and enhanced linear electron transport by elevating electron transport from QA to PSI. The B treatment plants exhibited normal photosystems activity and photosynthetic electron transport capacity similar to that of the Fl treatment. It was concluded that blue light is more essential than red light for normal photosynthesis by mediating photosystems activity and photosynthetic electron transport capacity.

关键词： cucumber photosystem II photosystem I electron transport

Ferroelectric effect and equivalent polarization charge model of PbZr_(0.2)Ti_(0.8)O_(3)on AlGaN/GaN MIS-HEMT

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Chinese Physics B 2021年第5期30卷 602-606页

作者： Yao-Peng Zhao Chong Wang Xue-feng Zheng Xiao-Hua Ma ang li Kai liu yun-long He Xiao-li lu Yue Hao Key laboratory of Wide Band-Gap Semiconductor Materials and Devices School of MicroelectronicsXidian UniversityXi'an 710071China

PbZr_(0.2)Ti_(0.8)O_(3)(PZT)gate insulator with the thickness of 30 nm is grown by pulsed laser deposition(PlD)in AlGaN/GaN metal-insulator-semiconductor high electron mobility transistors(MIS-HEMTs).The ferroelectric effect of PZT AlGaN/GaN MIS-HEMT is *** polarization charge in PZT varies with different gate *** equivalent polarization charge model(EPCM)is proposed for calculating the polarization charge and the concentration of two-dimensional electron gas(2DEG).The threshold voltage(V_(th))and output current density(I_(DS))can also be obtained by the *** theoretical values are in good agreement with the experimental results and the model can provide a guide for the design of the PZT *** polarization charges of PZT can be modulated by different gate-voltage stresses and the V_(th)has a regulation range of 4.0 *** polarization charge changes after the stress of gate voltage for several *** the gate voltage is stable or changes at high frequency,the output characteristics and the current collapse of the device remain stable.

关键词： PZT AlGaN/GaN MIS-HEMT equivalent polarization charge model(EPCM)