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麻醉与监护论坛 2012年第6期19卷 447-451页

作者： yun wang zong-ze zhang yun wu yan-lin wang Department of Anesthesiology Zhongnan Hospital of Wuhan University Wuhan 430071 China

一种新型的含筛孔八棱锥内构件泡沫分离设备的构建及其应用

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食品工业科技 2025年第1期46卷 104-113页

作者：薛静丽方思含张才亮韩娟吴嘉聪王蕾王赟江苏大学食品与生物工程学院江苏镇江212013 江苏大学化学化工学院江苏镇江212013

目的:本研究设计了一种新型的含筛孔八棱锥内构件,用以强化泡沫排液,提高泡沫分离效率。方法:以重组β-葡萄糖苷酶(Glu-linker-ELP50-GB,GLEGB)发酵液为模型,考察内构件的结构参数(个数、间距、筛孔直径)和泡沫分离实验参数(温度、初始... 详细信息

目的:本研究设计了一种新型的含筛孔八棱锥内构件,用以强化泡沫排液,提高泡沫分离效率。方法:以重组β-葡萄糖苷酶(Glu-linker-ELP50-GB,GLEGB)发酵液为模型,考察内构件的结构参数(个数、间距、筛孔直径)和泡沫分离实验参数(温度、初始蛋白浓度、气体流速、装液量)对GLEGB富集比和回收率的影响。此外,基于弹性蛋白样多肽(elastin-like polypeptides,ELPs)的温敏性,对重组β-葡萄糖苷酶泡沫液进一步纯化,并通过圆二色光谱仪(CD)、紫外-可见分光光度计(UV-vis)和傅里叶-红外光谱仪(FT-IR)对纯化后GLEGB的结构进行分析。结果:在内构件数量为5、间距为78 mm、筛孔直径为2 mm、蛋白质初始浓度为0.3 mg/mL、温度为45℃、气体流速为200 mL/min、装液量为100 mL的最佳条件下,GLEGB的蛋白富集比为2.46±0.10是对照柱的1.33倍,对重组β-葡萄糖苷酶泡沫液进一步纯化后GLEGB的纯化倍数为37.25±0.60,此外,结果证明纯化过程不会影响GLEGB的结构。结论:本研究设计的含筛孔八棱锥内构件可以有效地提高从发酵液中分离GLEGB的富集比,为泡沫分离设备的设计、研究及工艺优化提供了新的研究思路。

关键词：泡沫分离富集比重组 β-葡萄糖苷酶内构件

Understanding visual-auditory correlation from heterogeneous features for cross-media retrieval

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第2期9卷 241-249页

作者： Hong zhang yan-yun wang Hong PAN Fei wu College of Computer Science and Technology Wuhan University of Science and Technology Wuhan 430081 China School of Computer Science and Technology Zhejiang University Hangzhou 310027 China School of Elementary Education Hangzhou Normal University Hangzhou 310036 China School of Information Engineering Hangzhou Normal University Hangzhou 310036 China

Cross-media retrieval is an interesting research topic,which seeks to remove the barriers among different *** enable cross-media retrieval,it is needed to find the correlation measures between heterogeneous low-level features and to judge the semantic *** paper presents a novel approach to learn cross-media correlation between visual features and auditory features for image-audio retrieval.A semi-supervised correlation preserving mapping(SSCPM)method is described to construct the isomorphic SSCPM subspace where canonical correlations between the original visual and auditory features are further *** optimization algorithm is proposed to improve the local image cluster and audio cluster quality in an interactive way.A unique relevance feedback strategy is developed to update the knowledge of cross-media correlation by learning from user behaviors,so retrieval performance is enhanced in a progressive *** results show that the performance of our approach is effective.

关键词： Heterogeneity Cross-media retrieval Subspace optimization Dynamic correlation update

Bone marrow-derived dendritic cells pulsed with tumor lysates induce anti-tumor immunity against gastric cancer ex vivo

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World Journal of Gastroenterology 2008年第46期14卷 7127-7132页

作者： yan-lin Li Yu-Gang wu Yong-Qing wang Zhong Li Rong-Chao wang Liang wang yan-yun zhang Department of Paediatrics the First People Hospital of Changzhou and The Third Affiliated Hospital of Soochow University Changzhou 213000 Jiangsu Province China Department of Surgery the First People Hospital of Changzhou and The Third Affiliated Hospital of Soochow University Changzhou 213000 Jiangsu Province China Department of Surgery the First Affiliated Hospital of Sooehow University Suzhou 215006 Jiangsu Province China Institute of Health Sciences and Shanghai Institute of Immunology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

AIM： To investigate whether bone marrow-derived denritic cells pulsed with tumor lysates induce immunity against gastric cancer ex vivo. METHODS： c-kit＋ hematopoietic progenitor cells were magnetically isolated with a MiniMACS separator from BALB/c mice bone marrow cells. These cells were cultured with cytokines GM-CSF, IL-4, and TNFα to induce their maturation. They were analysed by morphological observation, phenotype analysis, and mixed lymphocyte reaction （MLR）. Bone marrowderived DCs （BM-DCs） were pulsed with tumor cell lysate obtained by rapid freezing and thawing at a 1：3 DC：tumor cell ratio. Finally, cytotoxic T lymphocyte （CTL） activity and interferon gamma （IFNγ） secretion was evaluated ex vivo. RESULTS： c-kit^＋ hematopoietic progenitor cells from mice bone marrow cells cultured with cytokines for 8 d showed the character of typical mature ***, observed by light microscope, these cells were large with oval or irregularly shaped nuclei and with many small dendrites. Phenotypically, FACS analysis showed that they *** levels of la, DEC-205, CD11b, CD80 and CD86 antigen, moderate levels of CD40, and negative for F4/80. Functionally, these cells gained the capacity to stimulate allogeneic T cells in MLR assay. However, immature DCs cultured with cytokines for 5 d did not have typical DCs phenotypic markers and could not stimulate allogeneic T cells. Ex vivo primed T cells with SGC-7901 tumor cell lysate-pulsed （TP） DCs were able to induce effective CTL activity against SGC-7901 tumor cells （E：T = 100：1, 69.55% ± 6.05% specific lysis）, but not B16 tumor cells, and produced higher levels of IFNγ, when stimulated with SGC-7901 tumor cells but not when stimulated with B16 tumor cells （1575.31 ± 60.25 pg/mL in SGC-7901 group vs 164.11± 18.52 pg/mL in B16 group, P 〈 0.01）. CONCLUSION： BM-derived DCs pulsed with tumor lysates Can induce anti-tumor immunity specific to gastric cancer ex vivo.

关键词： Dendritic cells Cytokine Gastric cancer Cytotoxic T lymphocyte Imrnunotherapy

Long-term potentiation-based screening identifies neuronal PYGM as a synaptic plasticity regulator participating in Alzheimer's disease

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Zoological Research 2023年第5期44卷 867-881页

作者： Ting wang yun-Qiang Zhou Yong wang Liang zhang Xiang Zhu Xiu-yan wang Jing-Hui wang lin-Kun Han Jian Meng Xian zhang Hong Luo Qi-lin Ma Zhan-Xiang wang yun-wu zhang Xiamen Key Laboratory of Brain Center The First Affiliated Hospital of Xiamen Universityand Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging ResearchInstitute of NeuroscienceSchool of MedicineXiamen UniversityXiamenFujian 361102China Fujian Provincial Clinical Research Center for Brain Diseases The First Affiliated Hospital of Xiamen UniversityXiamenFujian 361003China

Synaptic dysfunction is an important pathological hallmark and cause of Alzheimer's disease(AD).High-frequency stimulation(HFS)-induced long-term potentiation(LTP)has been widely used to study synaptic plasticity,with impaired LTP found to be associated with ***,the exact molecular mechanism underlying synaptic plasticity has yet to be completely *** genes regulating synaptic plasticity are altered in AD and contribute to disease onset also remains ***,we induced LTP in the hippocampal CA1 region of wildtype(WT)and AD model mice by administering HFS to the CA3 region and then studied transcriptome changes in the CA1 *** identified 89 genes that may participate in normal synaptic plasticity by screening HFS-induced differentially expressed genes(DEGs)in mice with normal LTP,and 43 genes that may contribute to synaptic dysfunction in AD by comparing HFS-induced DEGs in mice with normal LTP and AD mice with impaired *** further refined the 43 genes down to 14 by screening for genes with altered expression in pathological-stage AD mice without HFS *** them,we found that the expression of Pygm,which catabolizes glycogen,was also decreased in AD *** further demonstrated that down-regulation of PYGM in neurons impaired synaptic plasticity and cognition in WT mice,while its overexpression attenuated synaptic dysfunction and cognitive deficits in AD ***,we showed that PYGM directly regulated energy generation in *** study not only indicates that PYGM-mediated energy production in neurons plays an important role in synaptic function,but also provides a novel LTP-based strategy to systematically identify genes regulating synaptic plasticity under physiological and pathological conditions.

关键词： Alzheimer's disease High-frequency stimulation Long-term potentiation PYGM Synaptic plasticity Transcriptome

Distribution and features of landslides in the Tianshui Basin, Northwest China

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Journal of Mountain Science 2020年第3期17卷 686-708页

作者： zhang ze-lin wang Tao wu Shu-ren China Agricultural University Beijing 100083China Chinese Academy of Geological Sciences Institute of Geomechanics Beijing 100081China

Landslides in Tianshui Basin, Gansu Province, Northwest China, severely affect the local population and the economy;therefore,understanding their evolution and kinematics is of great interest for landslide risk assessment and prevention. However, there is no unified classification standard for the types of loess landslides in *** this study, we explored the landslide distribution and failure characteristics by means of field investigation,remotesensinginterpretation,geological mapping, drilling exploration and shearwave velocity tests, and established a database of Tianshui landslides. Our analysis shows that shear zones in mudstone usually develop in weak intercalated layers. Landslides occur mainly along the West Qinling faults on slopes with gradients of 10° to 25° and on southeast-and southwest-facing *** landslides were classified into five types: loess landslides, loess–mudstone interface landslides, loess flow-slides, loess–mudstone plane landslides and loess–mudstone cutting landslides. We discussed the evolution and failure process of each landslide type and analyzed the formation mechanism and motion characteristics of large-scale landslides. The analysis results show that the landslides in the study area are characterized by a gentle slope, long runout and high risk. The relationship between the runout L and the vertical drop H of the large-scale landslides in the study area is L > 4 H. There are good correlations between the equivalent friction coefficient of largescale landslides and their maximum height, runout,area and volume. The sliding zone of large-scale landslides often develops in the bedrock contact zone or in a weak interlayer within mudstone. From microstructure analysis, undisturbed mudstone consists mainly of small aggregates with dispersed inter-aggregate pores, whereas sheared clay has a more homogeneous structure. linear striations are well developed on shear surfaces, and the clay pores in those surfaces have a more uni

关键词： Loess Plateau Landslide distribution large-scale landslide landslide types slide distance Qinling fault

Construction of Electrocatalytic and Heat-Resistant Self-Supporting Electrodes for High-Performance Lithium–Sulfur Batteries

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Nano-Micro Letters 2019年第4期11卷 386-402页

作者： Xuemei zhang yunhong Wei Boya wang Mei wang yun zhang Qian wang Hao wu College of Materials Science and Engineering Sichuan UniversityChengdu 610064People’s Republic of China Department of Advanced Energy Materials Sichuan UniversityChengdu 610064People’s Republic of China

Boosting the utilization efficiency of sulfur electrodes and suppressing the“shuttle effect”of intermediate polysulfides remain the critical challenge for high-performance lithium-sulfur batteries(LSBs).However,most of reported sulfur electrodes are not competent to realize the fast conversion of polysulfides into insoluble lithium sulfides when applied with high sulfur loading,as well as to mitigate the more serious shuttle effect of polysulfides,especially when worked at an elevated ***,we reported a unique structural engineering strategy of crafting a unique hierarchical multifunctional electrode architecture constructed by rooting MOF-derived CoS2/carbon nanoleaf arrays(CoS2-CNA)into a nitrogen-rich 3D conductive scaffold(CTNF@CoS2-CNA)for *** accelerated electrocatalytic effect and improved polysulfide redox kinetics arising from CoS2-CNA were ***,the strong capillarity effect and chemisorption of CTNF@CoS2-CNA to polysulfides enable high loading and efficient utilization of sulfur,thus leading to high-performance LIBs performed not only at room temperature but also up to an elevated temperature(55°C).Even with the ultrahigh sulfur loading of 7.19 mg cm?2,the CTNF@CoS2-CNA/S cathode still exhibits high rate capacity at 55°C.

关键词： Metal organic framework Lithium-sulfur batteries Cobalt sulfide Heat-resistant N-doped carbon foam

Flow Control Effect of Spanwise Distributed Pulsed Arc Discharge Plasma Actuation on Supersonic Compressor Cascade Flow

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Journal of Thermal Science 2022年第5期31卷 1723-1733页

作者： SHENG Jiaming wu yun zhang Haideng wang Yizhou TANG Mengxiao Science and Technology on Plasma Dynamic Laboratory Air Force Engineering UniversityXi’an710038China Institute of Aero-engine School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an710049China

To achieve efficient control of supersonic compressor cascade flow,a type of spanwise distributed pulsed arc discharge plasma actuation(PADPA)was *** simulate the influences of PADPA on the flow field,a phenomenological model was ***,the flow control effects of PADPA on supersonic compressor cascade flow were researched *** results show that under low static pressure ratio condition,the compressive wave induced by PADPA reduced the intensity of the passage shock wave,which eventually reduced shock wave *** was also found that PADPA produced an adverse pressure gradient(pre-compression effect)around the actuation location,which reduced the strength of the high adverse pressure gradient induced by the passage shock *** airflow on both sides of the actuation location was accelerated by PADPA owing to the spanwise distributed ***,it improved the ability of the boundary layer to resist the effect of the adverse pressure gradient and reduced the separation ***,the total pressure loss was reduced by 6.8%.Under high pressure ratio condition,the effect of PADPA on the suction side controlling the large separation of the boundary layer was *** total pressure loss also increased slightly.

关键词： plasma flow control supersonic cascade shock wave/boundary layer interaction numerical simulation

High-pseudocapacitance of porous and square NiO@NC nanosheets for high-performance lithium-ion batteries

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Rare Metals 2021年第6期40卷 1451-1458页

作者： ze-lin Cai Zi-lin Peng Meng-Qi wang Jia-yan wu Hao-Sen Fan Yu-Fei zhang School of Chemistry and Chemical Engineering Guangzhou UniversityGuangzhou 510006China School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510006China

Layered nickel oxides have been focused with intense research interests as high-performance lithium-ion batterie(LIB)***,it is hard to obtain few layered nickel oxides material directly as it easily forms bulk material with the strong interaction between the *** this work,two-dimensional(2 D)nickel-based coordination polymers were successfully prepared according to aqueous phase copolymerization *** then uniform carbondoped NiO nanosheets were successfully obtained from facile solution assembly and post-thermal *** detailed electrochemical testing shows that the uniform NiO nanocrystals encapsulated into porous N-doped carbon(NiO@NC)nanosheets present much higher rate capability with the discharge specific capacity of 782.7 mAh·g^(-1) at high current density of 2.0 A·g^(-1) than pure NiO(690 mAh·g^(-1)).It also shows long-term cycling performance with 91%retention after 50 cycles at 1.0 A·g^(-1).The high rate capability,cycling stability and the easy synthesis make NiO@NC nanosheets as a promising candidate for LIB anode and build up new way for the fabrication of metal oxides anode materials.

关键词： Anode Batteries Square NiO@NC nanosheets Pseudocapacitive

Normal sperm head morphometric reference values in fertile Asian males

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Asian Journal of Andrology 2024年第3期26卷 315-320页

作者： Ye-lin Jia Ying-Bi wu lin Yu yan Zheng Ting-Ting yang yan-yun wang Bin Zhou lin zhang Fu-Ping Li Laboratory of Andrology West China Second University Hospital Human Sperm BankChengdu 610000China Laboratory of Molecular Translational Medicine Center for Translational MedicineChengdu 610061China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University) Ministry of EducationChengdu 610061China NHC Key Laboratory of Chronobiology West China Second University HospitalSichuan UniversityChengdu 610000China

Sperm head morphology is crucial for male factor infertility diagnosis and assessment of male reproductive *** are available to analyze sperm head morphology,but they are limited by poor methodology comparability and *** study aimed to explore comprehensive and new normal morphometric reference values for spermatozoa heads infertile Asian *** automated sperm morphology analysis system captured 23152 stained spermatozoa from confirmed *** these samples,1856 sperm head images were annotated by three experienced laboratory technicians as“normal”.Weemployed 14 novel morphometric features to describe sperm head size(head length,head width,length/width ratio,and girth),shape(ellipse intersection over union,girth intersection over union,short-axis symmetry,and long-axis symmetry),area(head,acrosome,postacrosomal areas,and acrosome area ratio),and degrees of acrosome and nuclear *** straight-forwardmethod for the morphometric analysis of sperm by accurate visual measurements is clinically *** measured parameterspresent valuable information to establish morphometric reference intervals for normal sperm heads in fertile Asian *** detailed measurement data will be valuable for interlaboratory comparisons and technician *** vitro fertilizationand andrology laboratory technicians can use these parameters to perform objective morphology evaluation when assessing malefertilization potential.

关键词： fertile Asian males head morphometric reference value normal spermatozoa