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Crystal reconstruction of V_(2)O_(3)/carbon heterointerfaces via anodic hydration for ultrafast and reversible Mg-ion battery cathodes

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InfoMat 2024年第3期6卷 95-107页

作者： Gun Jang yun sang joe sang Joon Lee Hyun Gyu Cho sang Ha Baek Peixun Xiong Kang Ho Shin Jeong Seok Yeon Min Su Kang Si Hyoung Oh Ho Seok Park School of Chemical Engineering Sungkyunkwan University(SKKU)SuwonRepublic of Korea Advanced Batteries Research Center Korea Electronics Technology InstituteSeongnamRepublic of Korea Korea Institute of Science and Technology SeoulRepublic of Korea SKKU Advanced Institute of Nano Technology(SAINT) Sungkyunkwan UniversitySuwonRepublic of Korea Department of Health Sciences and Technology Samsung Advanced Institute for Health Sciences and Technology(SAIHST)Sungkyunkwan UniversitySuwonRepublic of Korea SKKU Institute of Energy Science and Technology(SIEST) Sungkyunkwan UniversitySuwonRepublic of Korea

Magnesium-ion batteries(MIBs)have promising applications because of their high theoretical capacity and the natural abundance of magnesium ***,the kinetic performance and cyclic stability of cathode materials are limited by the strong interactions between Mg ions and the crystal ***,we demonstrate the unique Mg^(2+)-ion storage mechanism of a hierarchical accordion-like vanadium oxide/carbon heterointerface(V_(2)O_(3)@C),where the V_(2)O_(3) crystalline structure is reconstructed into a MgV_(3)O_(7)·H_(2)O phase through an anodic hydration reaction upon first cycle,for the improved kinetic and cyclic *** verified by in situ/ex situ spectroscopic and electrochemical analyses,the fast charge transfer kinetics of the V_(2)O_(3)@C cathode were due to the crystal-reconstruction and chemically coupled *** V_(2)O_(3)@C demonstrated an ultrahigh rate capacity of 130.4 mAh g^(-1)at 50000 mA g^(-1)and 1000 cycles,achieving a Coulombic efficiency of 99.6%.The high capacity of 381.0 mA h g^(-1)can be attributed to the reversible Mg^(2+)-ion intercalation mechanism observed in the MgV_(3)O_(7)·H_(2)O phase using a 0.3 M Mg(TFSI)2/ACN(H_(2)O)***,within the voltage range of 2.25 V versus Mg/Mg^(2+),the V_(2)O_(3)@C exhibited a capacity of 245.1 mAh g^(-1)when evaluated with magnesium metal in a 0.3 M Mg(TFSI)^(2+)0.25 M MgCl_(2)/DME *** research findings have important implications for understanding the relationship between the Mg-ion storage mechanism and reconstructed crystal phase of vanadium oxides as well as the heterointerface reconstruction for the rational design of MIB cathode materials.

关键词： anodic hydration reaction magnesium ion battery nano crystal vanadium oxide

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Efficient nanoparticle-based CRISPR-Cas13d induced mRNA disruption of an eye pigmentation gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2023年第6期30卷 1552-1564页

作者： yun-Feng Ma Meng-Qi Zhang Lang-Lang Gong Xuan-Zheng Liu Gui-Jun Long Huan Guo J.joe Hull Youssef Dewer Ming He Peng He National Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina USDA-ARS Arid Land Agricultural Research Center MaricopaAZUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt

The discovery of the clustered regularly interspaced short palindromic repeat(CRISPR)system has driven gene manipulation technology to a new era with applications reported in organisms that span the tree of *** utility of CRISPR-mediated editing was further expanded to mRNA following identification of the RNA-targeting Cas13 family of smaller endonuclease *** of this family to insect research,however,has been more *** this study,the smallest Cas13 family member,Cas13d,and guide RNAs(gRNAs)were complexed with a versatile nanomaterial(star polycation,SPc)to generate a proof-of-concept RNA-editing platform capable of disrupting mRNA expression of the eye pigmentation gene tryptophan 2,3-dioxygenase(SfTO)in white-backed planthoppers(WBPHs).The resulting red-eye phenotype was present in 19.76%(with SPc)and 22.99%(without SPc)of the treatment groups and was comparable to the red-eye phenotype generated following conventional RNA interference knockdown(22.22%).Furthermore,the Cas13/gRNA phenotype manifested more quickly than RNA *** with the expected Cas13d mechanism,SfTO transcript levels were significantly *** together,the results indicate that the SPc-CRISPR-Cas13d/gRNA complex negatively impacted expression of the target *** findings confirm the utility of this novel mRNA disruption system in insects and lay the foundation for further development of these tools in the implementation of green agricultural pest management tactics.

关键词： CRISPR-Cas13d RNA editing tryptophan 2,3-dioxygenase white-backed planthoppers

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Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

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Cancer Communications 2020年第4期40卷 135-153页

作者： Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim joe Yeong Tony Kiat Hon Lim Yong Loo Lin School of Medicine National University of SingaporeSingapore169856Singapore Department of Anatomical Pathology Singapore General HospitalSingapore169856Singapore Institute of Molecular Cell Biology(IMCB) Agency of ScienceTechnology and Research(A*STAR)Singapore169856Singapore Singapore Immunology Network Agency of Science(SIgN)Technology and Research(A*STAR)Singapore169856Singapore Duke-NUS Medical School Singapore169856Singapore

Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue ***,this technique is associated with a number of limitations,including high inter-observer variability and the capacity to label only one marker per tissue *** review details various highly multiplexed techniques that have emerged to circumvent these constraints,allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition,cellular functional and cell-cell *** these techniques,multiplex Immunohistochemistry/Immunofluorescence(mIHC/IF)has emerged to be particularly ***/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible,efficient and cost-effective tissue *** technique has immediate potential for translational research and clinical practice,particularly in the era of cancer immunotherapy.

关键词： immunofluorescence immunohistochemistry immunotherapy multiplex overview

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Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2024年第4期31卷 1015-1025页

作者： Meng-Qi Zhang Lang-Lang Gong Ya-Qin Zhao yun-Feng Ma Gui-Jun Long Huan Guo Xuan-Zheng Liu J.joe Hull Youssef Dewer Chao Yang Ning-Ning Zhang Ming He Peng He National Key Laboratory of Green Pesticides Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina Pest Management and Biocontrol Research Unit US Arid Land Agricultural Research CenterUSDA Agricultural Research ServicesMaricopaArizonaUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt Guizhou Jifeng Seed Industry Limited Liability Company XingyiGuizhou ProvinceChina Shandong Facility Horticulture Bioengineering Research Center Weifang University of Science and TechnologyWeifangShandong ProvinceChina

Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal *** is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice *** extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice *** bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult *** at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing *** evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 *** study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

关键词： CRISPR/Cas9 DIPA-CRISPR gene editing tryptophan 2,3-dioxygenase WBPH

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Principles and realizations of FBG wavelength tuning with elastic beams

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Optoelectronics Letters 2005年第1期1卷 5-9页

作者： ZHANG Wei-gang WU Zhi-gang LIU Yan-ge YANG Yi-fei KAI Gui-yun ZHAO Qi-da YUAN Shu-zhong DONG Xiao-yi LI En-bang XI Jiang-tao joe Chicharo Institute of Modern Optics Nankai University Tianjin 30071 China School of Electrical Computer and Telecommunication Engineering Faculty of Informatics University of Wollongong NSW 2522 Australia

The general principles and realizations of FBG wavelength tuning with elastic beams are proposed and *** and experiments show that when displacement at the center point of simple beams,deflection of cantilever beams and torsion strain of torsion beams are relatively small,Bragg wavelength shifts of sensing FBGs have linear relationship with applied external stress,lateral displacement,torque and torsional angles,*** experimental results indicate that the curvature sensitivity of the simple beam is 1.65 nm/m-1,the displacement sensitivity of the equivalent-strain cantilever beam is 4.4 cm/kg and the torque and torsional angle sensitivities of the torsion beam are 6.27 nm/Nm and 0.086 7 nm/degree,respectively.

关键词： Bragg波长弹性波束光纤灵敏度曲率

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Carnivalesque Features in joe Orton’s What the Butler Saw

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海外英语 2019年第23期 212-214页

作者：宫昀 Xianyang Normal University

joe Orton’s play What the Butler Saw is featured with a strong sense of *** can be made between the carnivalesque in this play and Rabelaisian carnivalesque life which Bakhtin delineates in Rabelais and His *** in Orton’s play have ambiguous sexual identities and dubious psychological states,and are unconscious of the desperate life they *** pseudo carnivalesque life,in a state of anarchic insanity,suggests Orton’s revolt against the rigid social institutions and his call for genuine mass democracy in the welfare society,nevertheless he is not optimistic about that if considering the religious path he indicates at the end of the play.

关键词： carnivalesque features sexual identity psychological states

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肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术

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癌症 2021年第9期40卷 367-387页

常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单... 详细信息

常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合研究。在这些技术中,多重免疫组织化学/免疫荧光(multiplex immunohistochemistry/immunofluorescence,mIHC/IF)已成为最具应用前景的技术。mIHC/IF为开展高重复性、高效、高性价比的组织研究提供了高通量多重染色技术和标准化的定量分析。该技术在转化研究和临床实践中,尤其是肿瘤免疫治疗中极具应用价值。

关键词：免疫荧光免疫组织化学免疫治疗多重概述

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政治地理学中的领域概念辨析

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人文地理 2019年第1期34卷 14-19页

作者：刘云刚王丰龙华南师范大学地理科学学院广州5106311 华东师范大学中国现代城市研究中心上海200062 华东师范大学城市发展研究院上海200062

领域是政治地理学的重要概念,但是目前国内对领域的内涵缺乏深入的解读和整理。本文在梳理西方对领域概念的理论探讨的基础上,归纳了理解领域概念的三个主要视角,并基于西方相关理论讨论,提出了领域概念的四个基本属性,即多尺度性、多... 详细信息

领域是政治地理学的重要概念,但是目前国内对领域的内涵缺乏深入的解读和整理。本文在梳理西方对领域概念的理论探讨的基础上,归纳了理解领域概念的三个主要视角,并基于西方相关理论讨论,提出了领域概念的四个基本属性,即多尺度性、多维性、关系性和生成性。继而,通过对西方领域理论的研究,提出未来中国政治地理学的领域研究应该加强的几个方向。本文对于深化政治地理学领域概念的理解,避免传统认识中的偏见和局限,以及更好地制定领域策略、展开领域研究将起到积极的推动作用。

关键词：领域尺度关系政治地理空间的生产

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拜登气候与能源政策主张对我国影响分析及对策建议

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世界科技研究与发展 2021年第5期43卷 605-615页

作者：汤匀陈伟中国科学院武汉文献情报中心武汉430071 科技大数据湖北省重点实验室武汉430071 中国科学院大学经济与管理学院北京100190

拜登作为新一任美国总统十分重视气候变化,制定了“清洁能源革命计划”来应对气候危机,宣布重返《巴黎气候协定》,提出2050年碳中和目标。本文对拜登“清洁能源革命计划”及气候政策主张等要点进行解读,结合对美国能源政策发展历程的回... 详细信息

拜登作为新一任美国总统十分重视气候变化,制定了“清洁能源革命计划”来应对气候危机,宣布重返《巴黎气候协定》,提出2050年碳中和目标。本文对拜登“清洁能源革命计划”及气候政策主张等要点进行解读,结合对美国能源政策发展历程的回溯分析,深入探究拜登政府的气候能源主张。研究发现,拜登在能源政策方面延续了民主党一贯的立场和主张,支持清洁能源革命,其基本立场与奥巴马时期无异,但有两方面的重要变化:一是美国已实现能源独立,将给拜登政府以及之后数届政府的内政外交政策选择带来更大的回旋空间,战略手段上更少受到能源因素的制约;二是拜登政府将更注重清洁能源技术创新的核心作用,借助美国掌握的源头创新优势和科技领导地位,主导制定一套新的游戏规则改革新形势下的国际能源治理体系,牢牢把控国际话语权以获得最大利益,在利用科技优势打压主要竞争对手上力度会进一步加强。随后分析了其对世界能源格局和对我国未来发展的影响,主要包括:1)气候变化认知和行为差异将造成未来中美摩擦;2)对中国的警惕将加剧美国与中国在气候相关创新技术领域的竞争;3)形成“民主国家”联盟对中国持续施压。对此,提出了我国应对建议:因应“双循环”新发展格局,加速推进清洁能源关键技术自立自强;加快建立国家级多能融合自主创新示范区,为我国实现碳达峰、碳中和目标贡献解决方案;深度融合数字技术与能源技术,构建我国智慧能源体系;深度参与全球合作,积极参与国际能源治理体系改革,为我国能源转型营造良好的外部环境;重启新篇开启中美合作新模式,共担责任引领推动全球能源绿色低碳转型和应对气候变化。

关键词：拜登气候与能源政策主张清洁能源革命计划能源格局碳中和对策建议

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曹魏铜雀台考

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北京林业大学学报（社会科学版） 2020年第3期19卷 79-83页

作者：胡运宏南京林业大学风景园林学院江苏省南京市210037

曹魏铜雀台有深厚的文化内涵。首先,在曹操逐步篡夺政权的过程中,铜雀台发挥着重要的政治喻示功能。其次,以铜雀台为核心的铜雀园又称西园,是当时作为我国历史上第一批真正文人的建安诸子们宴会雅集的场所,西园雅集称得上是后世文人园... 详细信息

曹魏铜雀台有深厚的文化内涵。首先,在曹操逐步篡夺政权的过程中,铜雀台发挥着重要的政治喻示功能。其次,以铜雀台为核心的铜雀园又称西园,是当时作为我国历史上第一批真正文人的建安诸子们宴会雅集的场所,西园雅集称得上是后世文人园林雅集的滥觞。第三,铜雀台(园)经过历代文人不断题咏,文学意象也经历了从"高台"到"铜雀伎",再到"深锁二乔"的流变与转型。

关键词：铜雀台天子三台西园雅集铜雀歌伎

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