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The 15th World veterinary Poultry Congress

作者： yumei cai~1 tongjie chai~(1*) xiaoli niu~2 1.college of animal science and veterinary medicine Shandong Agricultural University Taian 271018 PR.China. 2.college of foreign language Shandong Agricultural University Taian 271018 PR.China

＜正＞The fecal samples of 30-day AA broilor chickens were examined bacteriologically and by polymerase chain reaction（PCR）for occurrence of *** were examined by multie-polymerase chain re- action for genes encoding...

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Studies of induced colibacillosis in chickens

Studies of induced colibacillosis in chickens

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The 15th World veterinary Poultry Congress

作者： cai yumei~1 chai tongjie~1 Wang Xiaodong~2 1.college of animal science and veterinary medicine Shandong Agricultural University Taian 271018 PR.China 2.college of Plant Protection Shandong Agricultural University Taian 271018 PR.China

＜正＞Colibacillosis can be reproduced experimentally by inoculation of certain serogroups of Escherichia coli into ***,the severity of illness induced may vary,being influenced by many factors,one of which is the dos...

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Spread of *** Aerosols Carrying Antibiotic-Resistant Genes （Including MRSA） from Chicken Farms to Their Neighborhood Residents Environment

Spread of *** Aerosols Carrying Antibiotic-Resistant Genes ...

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2012年环境污染与大众健康学术会议

作者： Dunjiang Liu 1 tongjie chai 1, * yumei cai 1 Lingyu Sun 1 Haiyu Hao 1 Jian Wang 2 1.college of animal science and veterinary medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonoses of animal Origin of Shandong Province, Tai’an, 271018, China State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084 Beijing, China. 2 Bureau of animal Husbandry and veterinary Zouping County, Shandong Province, China.

There is a rather limited understanding concerning the antibiotic-resistance of the airborne S. aureus and the transmission of the antibiotic-resistant genes it carries Therefore, we isolated 149 S. aureus strains from the samples collected from the feces, the indoor air and the outdoor air of 6 chicken farms, and performed the research on them with 15 types of antibiotics and the REP-PCR trace identification. The 100% homologous strains were selected to conduct the research on the carrying and transmission status of the antibiotic-resistant genes. The results revealed that 5.37% strains（8/149） were resistant to methicillins （MRSA）, and 94% strains （140/149） were resistant to compound sulfamethoxazole, etc. In addition, these strains displayed a resistance to multiple antibiotics （4, 5 or 6 types） and there were also 3 strains resistant to 9 antibiotics. the strains with the same REP-PCR trace identification result carried the same type of antibiotic-resistant genes. The results showed that airborne transmission not only causes the spread of epidemic diseases but also exerts threats to the public health of a community.

关键词： chicken farming environment aerosols of Staphylococcus aureus including MRSA REP-PCR tracing antibiotic-resistance antibiotic-resistant genes significance to epidemiology and public health.

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Transmission identification of Escherichia coli aerosol in chicken houses to their environments using ERIC-PCR

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science China(Life sciences) 2008年第2期51卷 164-173页

作者： DUAN HuiYong chai tongjie cai yumei ZHONG ZhaoBing YAO MeiLing ZHANG XingXiao college of animal Husbandry and veterinary medicine Shandong Agricultural UniversityTai'an 271018China

In order to study E. coli aerosol spreading from chicken houses to their surrounding air, air samples, including indoor and outdoor air (upwind 10 and 50 m as well as downwind 10, 50, 100, 200 and 400 m away) of 5 chicken houses were collected using six-stage Andersen microbial samplers and Reuter-Centrifugal samplers (RCS). E. coli concentrations (CFU/m3 air) collected from different sampling sites were calculated. E. coli strains from chicken feces samples were also isolated. Furthermore, the enterobacterial repetitive intergenic consensus (ERIC)-PCR method was applied to amplify the isolated E. coli strain DNA samples. Through the genetic similarity analyses of the E. coli obtained from different sampling sites, the spreading of bioaerosol from animal houses to the ambient air was characterized. The results showed that the isolated E. coli concentrations in indoor air (9―63 CFU/m3) in 5 chicken houses were higher than those in upwind and downwind air, but there were no significant differences between the indoor and downwind sites 10 m away from all the 5 houses (P>0.05). The phylogenetic tree indicated that a part of the E. coli (34.1%) isolated from indoor air had 100% similarity with those isolated from feces, and that most of E. coli isolated (54.5%) from downwind at 10, 50, 100 or even 200 m had 100% similarity with those isolated from indoor air or feces too. But those isolated from upwind air had a lower similarity (73%―92%) with corresponding strains isolated from indoor air or feces. Our results suggested that some strains isolated from downwind air and indoor air originated in the chicken feces, but most of isolates obtained from upwind air samples did not come from the chicken feces or indoor air. Effective hygienic measures should be taken in animal farms to prevent or minimize downwind spreading of microorganism aerosol.

关键词： chicken house spread of E.coli aerosol ERIC-PCR similarity identification epidemiological significance

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The expression of immune-related gene of ducks infected with Avian pathogenic Escherichia coli(APEC)

The expression of immune-related gene of ducks infected with...

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中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会

作者： Rong Li Ning Li Jinzhou Zhang Yao Wang Jiyuan Liu yumei cai tongjie chai Liangmeng Wei college of animal science and veterinary medicine Shandong Agricultural University Sino-German Cooperative Research Centre for Zoonosis of animal Origin Shandong Province Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Diseases of Taishan Medical college

Avian pathogenic Escherichia coli(APEC) can cause a serious disease in ducks,characterized by perihepatitis,pericarditis and *** the studies of bacteria isolation and methods of detection have been already reported,the host immune responses to APEC infection were still *** this research,we examined the expression of immune-related genes and bacteria distribution in APEC-infected ducks *** results demonstrated that APEC could quickly replicate in multiple tissues with the highest bacteria content in the spleen at 2 day post infection at early *** expressions of Toll-like receptors(TLRs),avian P-defensins(AvBDs) and Major histocompatibility complex(MHC) were tested in the liver,spleen and brain of the infected ***2,TLR4,TLRS and TLR15 showed different expression pattern,which indicated they were all response to APEC *** expression of AvBD2 was up-regulated in all the tested tissues,whereas the expression of AvBD4,AvBD5,AvBD7 and AvBD9 were down-regulated during three tested ***,MHC-Ⅰ was up-regulated at all tested days,but MHC-Ⅱ was dramatically *** study suggested that APEC could replicate in various tissues in a short time,and the activation of host immune responses begins at the initial time of *** results were useful to understand the immune responses of ducks infection with APEC and put insight into pathogenesis of APEC due to host facts.

关键词： Cherry Valley ducks APEC Host immune responses TLRs AvBDs MHCs

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Pathogenicity of duck plague and innate immune responses of the Cherry Valley ducks to duck plague virus

Pathogenicity of duck plague and innate immune responses of ...

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中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会

作者： Ning Li Tianqi Hong Rong Li Mengjiao Guo Yao Wang Jinzhou Zhang Jiyuan Liu yumei cai Sidang Liu tongjie chai Liangmeng Wei college of animal science and veterinary medicine Shandong Agricultural UniversitySino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Diseases of Taishan Medical college

Duck plague caused by duck plague virus(DPV) is an acute and contagious *** better understand the pathogenic mechanism of duck plague virus in ducklings,an infection experiment was *** results showed that typical symptoms were observed in the infected *** could replicate quickly in many tissues,leading to pathological lesions,especially on the ***-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the brain,and the antiviral innate immune response was established,but not sufficient to restrict viral *** contrast,although the expression of many major pattern recognition receptors(PRRs) increased in the spleen,the expression of most cytokines was *** study indicates that DPV is a pantropic virus that can replicate rapidly in tissues,causing serious pathological lesions but the immune responses are different in the spleen and *** our knowledge,this is the first report to systematically explore the expression profiles of the immune genes in the DPV-infected *** data provide a foundation for further study of the pathogenicity of duck plague.

关键词： duck plague virus Cherry Valley ducklings pathogenicity innate immunity

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破窗效应之“破”——基于小农户生猪粪污治理技术使用态度的考察

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黑龙江畜牧兽医（下半月） 2017年第9期 7-12页

作者：王晓莉徐娜朱秋鹰谢玉梅陆姣江南大学商学院江苏无锡214122 江南大学江苏省食品安全研究基地江苏无锡214122 石河子大学信息科学与技术学院新疆石河子832003

以江苏省654家不同村庄的生猪养殖户为样本,从破窗效应情境分析村域小农户是否采用粪污治理技术的不同后果;结合使用统一理论模型(UTAUT)考察影响小农户生猪粪污治理技术使用态度的主要因素,探讨农村环境污染的破窗效应如何"破"题。结... 详细信息

以江苏省654家不同村庄的生猪养殖户为样本,从破窗效应情境分析村域小农户是否采用粪污治理技术的不同后果;结合使用统一理论模型(UTAUT)考察影响小农户生猪粪污治理技术使用态度的主要因素,探讨农村环境污染的破窗效应如何"破"题。结果表明:社会影响、绩效预期、努力预期、便利条件和养殖户特征均显著影响养殖户粪污治理技术使用态度。其中,社会影响和绩效预期分列前两位。破窗效应之"破",首先可以考虑通过具有共同经济利益诉求的村域社会网络,利用"精英人物"的影响力,与政府支持相配合,引导小农户使用生猪粪污治理技术。

关键词：破窗效应小农户生猪粪污治理技术技术使用态度

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