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An inducible CRISPR activation tool for accelerating plant regeneration

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Plant Communications 2024年第5期5卷 109-122页

作者： Cuimei Zhang Yajun Tang Shanjie Tang Lei Chen Tong Li Haidi Yuan yujun xu Yangyan Zhou Shuaibin Zhang Jianli Wang Hongyu Wen Wenbo Jiang Yongzhen Pang Xian Deng Xiaofeng Cao Junhui Zhou Xianwei Song Qikun Liu State Key Laboratory of Protein and Plant Gene Research School of Advanced Agricultural SciencesPeking UniversityBeijing 100871China Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences at WeifangShandong 261000China National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences Heilongjiang 150086China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Seed Innovation and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

The inducible CRISPR activation(CRISPR-a)system offers unparalleled precision and versatility for regu-lating endogenous genes,making it highly sought after in plant *** this study,we developed a chem-ically inducible CRISPR-a tool for plants called ER-Tag by combining the LexA-VP16-ER inducible system with the SunTag CRISPR-a *** systematically compared different induction strategies and achieved high efficiency in target gene *** demonstrated that guide RNAs can be multiplexed and pooled for large-scale screening of effective morphogenic genes and gene pairs involved in plant *** experiments showed that induced activation of these morphogenic genes can accelerate regenera-tion and improve regeneration efficiency in both eudicot and monocot plants,including alfalfa,woodland strawberry,and *** study expands the CRISPR toolset in plants and provides a powerful new strategy for studying gene function when constitutive expression is not feasible or ideal.

关键词： inducible CRISPR activation morphogenic gene genetic transformation plant regeneration

中日双语的“混血性”与“陌生化”——从《我是猫》到《我似猫》

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当代外语研究 2024年第3期 140-149页

作者：林祁许玉军厦门大学嘉庚学院漳州363105 集美大学厦门361021

横山悠太的《我似猫》上承夏目漱石《我是猫》的日本近代文学传统,进而杂糅中日双语而创造出崭新的文体,成为当代中日文学关系中的跨界奇作。这是一部与夏目漱石《我是猫》、鲁迅《阿Q日记》一脉相承的批判性杰作,其“混血性”的别样文... 详细信息

横山悠太的《我似猫》上承夏目漱石《我是猫》的日本近代文学传统,进而杂糅中日双语而创造出崭新的文体,成为当代中日文学关系中的跨界奇作。这是一部与夏目漱石《我是猫》、鲁迅《阿Q日记》一脉相承的批判性杰作,其“混血性”的别样文体先是在日语界引发热议,继而其中文版“不可翻译”的翻译又一次催生陌生化效果。它不仅具有中日之间的语言“移植”的实践意义,而且对文体创新具有意义。“混血性”与陌生化使语言文学充满生机,使读者在惊奇中获得新鲜的美学感受。

关键词：《我是猫》《我似猫》中日双语的“混血性” 陌生化

海南省艾滋病抗病毒治疗脱失情况及影响因素分析

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中国热带医学 2024年第3期24卷 304-308页

作者：冯玉婷许玉军朱考考张雯婷海南省第五人民医院(海南医学院附属皮肤病医院)防治科海南海口570100

目的了解海南省艾滋病病毒感染者/患者(human immunodeficiency virus/acquired immunodeficiency syn‐drome,HIV/AIDS)参加抗病毒治疗(antiretroviral therapy,ART)在治与脱失的现状,分析治疗脱失的影响因素。方法本研究选取中国疾病预防控制信息系统抗病毒治疗子模块中海南省2005—2022年启动ART治疗的HIV感染者,根据纳入和排除标准共纳入4286例研究对象,采用Cox比例风险回归模型分析其脱失情况及影响因素。结果4286例研究对象中,男性3718例(86.7%),性别比为6.55∶1;未婚者占58.4%;年龄为(39.68±13.17)岁;同性性传播占49.8%;WHO临床分期Ⅰ期占84.3%;治疗方案含依非韦伦(efavirenz,EFV)方案占71.7%。共随访19677.44人年,总体脱失率为0.80/100人年,开始抗病毒治疗第1年内脱失率为21.10/100人年。Cox回归分析显示,开始治疗时间为2016—2022年(AHR=2.40,95%CI:1.40~4.10),最近一次HIV病毒载量(virus load,VL)检测值20~<1000 copies/mL(AHR=3.69,95%CI:2.08~6.54),最近一次VL检测值≥1000 copies/mL(AHR=15.98,95%CI:9.46~27.01),最近一次VL未检测(AHR=92.90,95%CI:57.68~149.62),确诊到治疗的时间间隔1~12月(AHR=1.62,95%CI:1.12~2.36),间隔大于12月(AHR=1.68,95%CI:1.07~2.62)是使HIV感染者接受抗病毒治疗后脱失风险增加的因素。治疗方案含洛匹那韦/利托那韦(lopinavir/ritonavir,Lpv/r)(AHR=0.34,95%CI:0.18~0.66)、含整合酶抑制剂(integrase strand transfer inhibitors,INSTIs)方案(AHR=0.24,95%CI:0.09~0.58)是使HIV感染者接受抗病毒治疗后脱失的风险降低的因素。结论海南省艾滋病患者抗病毒治疗出现脱失与确诊到治疗的时间间隔长、治疗方案、HIV病毒载量检测结果异常有关。应针对影响抗病毒治疗脱失的因素采取个案化的措施,同时提升抗病毒治疗及时性及治疗管理服务质量,进一步提高抗病毒治疗效果。

关键词：艾滋病抗病毒治疗脱失影响因素

海南省HIV感染者/AIDS患者抗病毒治疗免疫学效果及其影响因素

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中国热带医学 2024年第5期24卷 561-565,578页

作者：冯玉婷许玉军乔凤朱考考张雯婷海南省第五人民医院(海南医学院附属皮肤病医院)防治科海南海口570100

目的了解海南省艾滋病病毒感染者/艾滋病患者(human immunodeficiency virus/acquired immunodeficiency syndrome,HIV/AIDS)参加高效抗逆转录病毒治疗(highly active antiretroviral therapy,HAART)的状况,分析其治疗后免疫学效果及其影响因素。方法本研究选取中国疾病预防控制信息系统抗病毒治疗子模块中海南省截至2022年治疗的HIV/AIDS患者,根据纳入和排除标准共纳入3150例研究对象,采用logistic回归分析影响免疫学效果的因素。结果3150例研究对象中,发生免疫学成功2906例(占92.2%);治疗后最近一次CD4^(+)T淋巴细胞计数M(P25,P75)为491(351,656)个/μL,不同水平的CD4^(+)T淋巴细胞计数组间治疗后与基线增加值的差异有统计学意义(P<0.001)。Logistic多因素分析结果显示:离异或丧偶(OR=0.50,95%CI:0.31~0.79),开始治疗时年龄≥60岁(OR=0.51,95%CI:0.29~0.90),确诊到治疗的时间间隔1~<12个月(OR=0.55,95%CI:0.40~0.77),确诊到治疗的时间间隔12~<60个月(OR=0.53,95%CI:0.34~0.80),确诊到治疗的时间间隔≥60月(OR=0.41,95%CI:0.23~0.75),CD4^(+)T淋巴细胞计数300~<400个/μL组(OR=0.36,95%CI:0.22~0.58),CD4^(+)T淋巴细胞计数400~<500个/μL组(OR=0.30,95%CI:0.18~0.49)与发生免疫学成功呈负相关(P<0.05)。而同性传播(OR=3.46,95%CI:2.03~5.90),异性传播(OR=1.91,95%CI:1.14~3.19),其他传播途径(OR=3.13,95%CI:1.50~6.53),治疗时长2~<4年(OR=1.71,95%CI:1.13~2.58),治疗时长4~<6年(OR=2.86,95%CI:1.81~4.51),治疗时长≥6年(OR=2.21,95%CI:1.47~3.32)与发生免疫学成功呈正相关(P<0.05)。结论海南省HIV/AIDS患者抗病毒治疗后取得较好的免疫学效果,应扩大HIV监测检测,早发现早治疗,持续加强服药依从性教育,进一步提升治疗免疫学效果。

关键词：艾滋病抗病毒治疗免疫学效果影响因素

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纳米除草剂的应用与环境安全性评估

世界农药 2024年第6期46卷 9-15页

作者：邓亚男徐榆钧邓希乐湖南省农业科学院湖南省农业生物技术研究所长沙410125 贵州大学精细化工研究开发中心绿色农药国家重点实验室绿色农药与农业生物工程教育部重点实验室贵阳550025

纳米技术在农业领域应用迅速兴起。纳米除草剂是基于纳米材料加工的植物保护产品。概述了有机纳米材料、无机纳米材料、杂化纳米材料及其在纳米除草剂中的应用,分析了纳米除草剂特点;初步分析了纳米除草剂发挥除草作用特点,探讨了纳米... 详细信息

纳米技术在农业领域应用迅速兴起。纳米除草剂是基于纳米材料加工的植物保护产品。概述了有机纳米材料、无机纳米材料、杂化纳米材料及其在纳米除草剂中的应用,分析了纳米除草剂特点;初步分析了纳米除草剂发挥除草作用特点,探讨了纳米除草剂在植物中的命运、行为和缺陷。旨在为环境友好型除草剂产品的可持续发展提供参考。

关键词：纳米除草剂杂草控制纳米材料农业应用环境风险评估

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真菌诱导子调控紫草素合成的分子机制探究

中国农业科技导报 2024年第1期26卷 78-88页

作者：李相吴刘自扬徐玉俊祝建波吴燕民石河子大学生命科学学院新疆石河子832003 中国农业科学院生物技术研究所北京100081 兰州大学草地农业科技学院兰州大学农业农村部草牧业创新重点实验室兰州730020

为探讨真菌诱导子调控紫草素合成的分子机制,以新疆紫草无菌苗为试材,经尖孢镰刀菌、立枯丝核菌制备成的诱导子生物诱导后,对其根部进行RNA测序和分析。结果表明,与对照组比较,尖孢镰刀菌试验组差异表达基因1735个;立枯丝核菌试验组差... 详细信息

为探讨真菌诱导子调控紫草素合成的分子机制,以新疆紫草无菌苗为试材,经尖孢镰刀菌、立枯丝核菌制备成的诱导子生物诱导后,对其根部进行RNA测序和分析。结果表明,与对照组比较,尖孢镰刀菌试验组差异表达基因1735个;立枯丝核菌试验组差异表达基因1043个。GO(gene ontology)分析发现,2个试验组差异表达基因主要富集在细胞过程、细胞组分中膜和分子功能中催化活性等生物学过程。KEGG(kyoto encyclopedia of genes and genomes)分析发现,2个试验组在植物与病原菌互作、植物激素信号转导途径和苯丙素合成等通路均有大量差异表达基因富集。2个试验组差异表达情况相同的转录因子主要包括bHLH、AP2/ERF-ERF和LOB等,并发现参与紫草素合成及其正向调控的AeGHQH、AeDSH1、AeAP、AePAL、AeDI2、AePGT、AeHMGR、AeG10H基因在2个试验组均上调表达,其中尖孢镰刀菌试验组上调表达较显著。以上结果从分子水平探究了新疆紫草对真菌诱导子生物诱导的响应机制,为未来真菌诱导子应用于新疆紫草种植生产奠定了理论基础。

关键词：新疆紫草真菌诱导子转录组差异表达基因

Water-conserving mining influencing factors identification and weight determination in northwest China

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International Journal of Coal Science & Technology 2019年第1期6卷 95-101页

作者： Limin Fan Liqiang Ma Yihe Yu Shuokang Wang yujun xu Shaanxi Institute of Geo-Environment Monitoring Xi'an 710054China School of Energy Xi'an University of Science and TechnologyXi'an 710054 Shaanxi China School of Mines China University of Mining and TechnologyXuzhou 221116JiangsuChina

Water-conserving mining is an effective way to alleviate the contradiction between fragile ecological environment and high-intensity coal mining in the arid and semi-arid region of northwest China. It needs to consider the engineering and geological conditions, hydrogeological conditions and mining methods of coal seams. From the three aspects, this paper systematically analyzes the influencing factors and establishes an identification model with multi-level structures. The model includes three primary factors (including the engineering and geological conditions, hydrogeological conditions and mining methods), nine secondary factors (including overlying strata thickness, aquiclude, mining parameters and etc.), sixteen third-tier factors (including the faults, aquiclude thickness and effective mining height and etc.) and twelve fourth-tier factors (including the fault throw exp on ent, aquiclude permeability and coal pillar sizes and etc.). On the basis, the analytic hierarchy process is used to build the judgment matrix and obtain the weight of each influencing factor. The results indicate that the overlying strata thickness, aquiclude and effective mining height are the most import a nt factors among the primary factors of engineering and geological conditions, hydrogeological conditions and mining methods, respectively. The research results could provide theoretical references for the water-conserving mining of coal resources in northwest China.

关键词： Water-conserving mining In flue ncing factors Analytic hierarchy process Judgment matrix Weight

DAZL is a master translational regulator of murine spermatogenesis

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National Science Review 2019年第3期6卷 455-468页

作者： Haixin Li Zhuqing Liang Jian Yang Dan Wang Hanben Wang Mengyi Zhu Baobao Geng Eugene yujun xu State Key Laboratory of Reproductive Medicine Nanjing Medical University

Expression of DAZ-like(DAZL) is a hallmark of vertebrate germ cells, and is essential for embryonic germ cell development and differentiation, yet the gametogenic function of DAZL has not been fully characterized and most of its in vivo direct targets remain unknown. We showed that postnatal stage-specific deletion of Dazl in mouse germ cells did not affect female fertility, but caused complete male sterility with gradual loss of spermatogonial stem cells, meiotic arrest and spermatid arrest. Using the genome-wide high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation and mass spectrometry approach, we found that DAZL bound to a large number of testicular m RNA transcripts(at least 3008) at the 3-untranslated region and interacted with translation proteins including poly(A) binding protein. In the absence of DAZL, polysome-associated target transcripts, but not their total transcripts, were significantly decreased, resulting in a drastic reduction of an array of spermatogenic proteins and thus developmental arrest. Thus, DAZL is a master translational regulator essential for spermatogenesis.

关键词： RNA binding proteins CLIPs infertility translational regulation DAZ

PUM1 represses CDKN1B translation and contributes to prostate cancer progression

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The Journal of Biomedical Research 2021年第5期35卷 371-382页

作者： Xin Li Jian Yang Xia Chen Dandan Cao Eugene yujun xu State Key Laboratory of Reproductive Medicine Nanjing Medical UniversityNanjingJiangsu 211166China Department of Urology the Second Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 211166China Department of Neurology Feinberg School of MedicineNorthwestern UniversityChicagoIL 60611USA

Posttranscriptional regulation of cancer gene expression programs plays a vital role in carcinogenesis;identifying the critical regulators of tumorigenesis and their molecular targets may provide novel strategies for cancer diagnosis and *** conserved RNA-binding protein Pumilio-1(PUM1)regulates mouse growth and cell proliferation,propelling us to examine its role in *** found human PUM1 is highly expressed in a diverse group of cancer,including prostate cancer;enhanced PUM1 expression is also correlated with reduced survival among prostate cancer *** expression analysis in twenty prostate cancer tissues showed enhanced expression of PUM1 at mRNA and protein *** of PUM1 reduced prostate cancer cell proliferation and colony formation,and subcutaneous injection of PUM1 knockdown cells led to reduced tumor *** of PUM1 in prostate cancer cells consistently elevated cyclin-dependent kinase inhibitor 1B(CDKN1B)protein expression through increased translation but did not impact its mRNA level,while overexpression of PUM1 reduced CDKN1B protein *** finding established a critical role of PUM1 mediated translational control,particularly the PUM1-CDKN1B axis,in prostate cancer cell growth and *** proposed that PUM1-CDKN1B regulatory axis may represent a novel mechanism for the loss of CDKN1B protein expression in diverse cancers and potential targets for therapeutics development.

关键词： PUM1,prostate cancer posttranscriptional regulation CDKN1B