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Expression and characterization of a novel GH128 laminarinase OUC-BsLam26 harboring both hydrolyzing and transferring activities

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Food Science and Human Wellness 2024年

作者： yueyang dong Yongyi Quan Hong Jiang Hamed I.Hamouda Zewei Lu Yimiao Chen Chengqiang Li Xiangzhao Mao State Key Laboratory of Marine Food Processing and Safety Control， College of Food Science and Engineering， Ocean University of China Qingdao Key Laboratory of Food Biotechnology Sanya Ocean Research Institute， Ocean University of China Processes Design and Development Department， Egyptian Petroleum Research Institute Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Biological Processing of Aquatic Products，China National Light Industry

Laminarin oligosaccharides (LOSs) with a specific degree of polymerization prepared through the laminarin degradation via laminarinase present more significant nutritional functions and application values. Human intestinal bacteria are promising potential producers of novel carbohydrate-active enzymes with unique properties. Here， a novel glycoside hydrolase family 128 (GH128) laminarinase OUC-BsLam26 from the intestinal bacterium Bacteroides sp. CBA7301 was heterologously expressed and characterized. The recombinant OUC-BsLam26 with a molecular mass of 49.86 kDa exhibits highest activity (6.60 U/mg) at 45 ℃ and pH 6.0， which shows noticeable temperature and pH stability. The purified OUC-BsLam26 could degrade laminarin via an endo-type mode with the generation of laminaripentaose， laminaritetraose， laminaritriose， and laminaribiose， among them， laminaritetraose is the principal product， which accounts for 45.25% of the total products， which is significantly different from the reported GH128 laminarinases. The minimum recognition substrate of OUC-BsLam26 is laminarihexaose. Furthermore， OUC-BsLam26 also could catalyze the transglycosylation process with the production of some novel glycosides. Altogether， the intestinal bacterium Bacteroides sp. CBA7301 contains laminarinase with unique product composition and OUC-BsLam26 is a hopeful bio-catalyst with the potential to produce laminaritetraose and some novel glycosides.

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Resonance mechanism of flapping wing based on fluid structure interaction simulation

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Chinese Journal of Aeronautics 2024年第5期37卷 243-262页

作者： yueyang GUO Wenqing YANG Yuanbo dong dong XUE National Key Laboratory of Science and Technology on Aerodynamic Design and Research School of AeronauticsNorthwestern Polytechnical UniversityXi'an 710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518063China Yangtze River Delta Research Institute of Northwestern Polytechnical University Taicang 215400China

Certain insect species have been observed to exploit the resonance mechanism of their *** order to achieve resonance and optimize aerodynamic performance,the conventional approach is to set the flapping frequency of flexible wings based on the Traditional Structural Modal(TSM)***,there exists controversy among researchers regarding the relationship between frequency and aerodynamic *** that the structural response of wings can be influenced by the surrounding air vibrations,an analysis known as Acoustic Structure Interaction Modal(ASIM)is introduced to calculate the resonant *** this study,Fluid Structure Interaction(FSI)simulations are employed to investigate the aerodynamic performance of flapping wings at modal frequencies derived from both TSM and ASIM *** performance is evaluated for various mass ratios and frequency ratios,and the findings indicate that the deformation and changes in vortex structure exhibit similarities at mass ratios that yield the highest aerodynamic ***,the flapping frequency associated with the maximum time-averaged vertical force coefficient at each mass ratio closely aligns with the ASIM frequency,as does the frequency corresponding to maximum ***,the ASIM analysis can provide an effective means for predicting the optimal flapping frequency for flexible ***,it enables the prediction that flexible wings with varying mass ratios will exhibit similar deformation and vortex structure *** paper offers a fresh perspective on the ongoing debate concerning the resonance mechanism of Flexible Flapping Wings(FFWs)and proposes an effective methodology for predicting their aerodynamic performance.

关键词： Flexible Flapping Wing(FFW) Acoustic Structure Interaction Modal(ASIM) Fluid Structure Interaction(FSI) Resonance mechanism Aerodynamic performance

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Enhanced autophagic clearance of amyloid-βvia histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer's disease in vitro and in vivo

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Neural Regeneration Research 2025年第9期20卷 2633-2644页

作者： Zhimin Long Chuanhua Ge yueyang Zhao Yuanjie Liu Qinghua Zeng Qing Tang Zhifang dong Guiqiong He Institute of Neuroscience School of Basic Medical SciencesChongqing Medical UniversityChongqingChina Department of Anatomy Chongqing Medical UniversityChongqingChina Department of Physiology Chongqing Medical UniversityChongqingChina Pediatric Research Institute Children’s Hospital of Chongqing Medical UniversityChongqingChina Ministry of Education Key Laboratory of Child Development and Disorders Children’s Hospital of Chongqing Medical UniversityChongqingChina National Clinical Research Center for Child Health and Disorders Children’s Hospital of Chongqing Medical UniversityChongqingChina Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders Children’s Hospital of Chongqing Medical UniversityChongqingChina

Recent studies have suggested that abnormal acidification of lysosomes induces autophagic accumulation of amyloid-βin neurons,which is a key step in senile plaque ***,resto ring normal lysosomal function and rebalancing lysosomal acidification in neurons in the brain may be a new treatment strategy for Alzheimer's *** acetylation/deacetylation plays a central role in lysosomal ***,we show that inhibiting the classic microtubule deacetylase histone deacetylase 6 with an histone deacetylase 6 shRNA or thehistone deacetylase 6 inhibitor valproic acid promoted lysosomal reacidification by modulating V-ATPase assembly in Alzheimer's *** rthermore,we found that treatment with valproic acid markedly enhanced *** clearance of amyloid-βaggregates,and ameliorated cognitive deficits in a mouse model of Alzheimer's *** findings demonstrate a previously unknown neuroprotective mechanism in Alzheimer's disease,in which histone deacetylase 6 inhibition by valproic acid increases V-ATPase assembly and lysosomal acidification.

关键词： Alzheimer's disease amyloid-β APP/PS1 mice autophagy cognitive impairment histone deacetylase 6 lysosomal acidification microtubule acetylation valproic acid V-ATPase

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高度人工化城市河流生态水位和生态流量计算方法

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水资源保护 2021年第1期37卷 140-144页

作者：华祖林董越洋褚克坚河海大学浅水湖泊综合治理与资源开发教育部重点实验室江苏南京210098 河海大学环境学院江苏南京210098

针对高度人工化城市河流生态水位和生态流量确定方法不成熟的问题,通过分析高度人工化城市河流特征和生态功能,解析了生态水位和生态流量的内涵,从维持河流连通性、水生生物生境、水质保障、城市景观娱乐功能等维度,提出了一种考虑河流... 详细信息

针对高度人工化城市河流生态水位和生态流量确定方法不成熟的问题,通过分析高度人工化城市河流特征和生态功能,解析了生态水位和生态流量的内涵,从维持河流连通性、水生生物生境、水质保障、城市景观娱乐功能等维度,提出了一种考虑河流形态、河道水生生物、水质和景观娱乐为要点的实用计算方法。以南京市秦淮河为案例,计算得到了秦淮河上段、秦淮河下段、外秦淮河和秦淮新河4个河段的生态水位分别为6.8 m、6.7 m、6.3 m和6.7 m,生态流量分别为20.8 m^3/s、25.8 m^3/s、26.5 m^3/s和19.1 m^3/s;计算结果介于Tennant标准的“好”和“非常好”之间,表明计算方法合理可信。

关键词：城市河流高度人工控制生态流量生态水位秦淮河

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Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer's disease in vitro and in vivo

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Neural Regeneration Research 2025年第09期 2633-2644页

作者： Zhimin Long Chuanhua Ge yueyang Zhao Yuanjie Liu Qinghua Zeng Qing Tang Zhifang dong Guiqiong He Institute of Neuroscience School of Basic Medical Sciences Chongqing Medical University Department of Anatomy Chongqing Medical University Department of Physiology Chongqing Medical University Pediatric Research Institute Children's Hospital of Chongqing Medical University Ministry of Education Key Laboratory of Child Development and Disorders Children's Hospital of Chongqing Medical University National Clinical Research Center for Child Health and Disorders Children's Hospital of Chongqing Medical University Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders Children's Hospital of Chongqing Medical University

Recent studies have suggested that abnormal acidification of lysosomes induces autophagic accumulation of amyloid-β in neurons,which is a key step in senile plaque ***,resto ring normal lysosomal function and rebalancing lysosomal acidification in neurons in the brain may be a new treatment strategy for Alzheimer's *** acetylation/deacetylation plays a central role in lysosomal ***,we show that inhibiting the classic microtubule deacetylase histone deacetylase 6 with an histone deacetylase 6 shRNA or thehistone deacetylase 6 inhibitor valproic acid promoted lysosomal reacidification by modulating V-ATPase assembly in Alzheimer's *** rthermore,we found that treatment with valproic acid markedly enhanced *** clearance of amyloid-β aggregates,and ameliorated cognitive deficits in a mouse model of Alzheimer's *** findings demonstrate a previously unknown neuroprotective mechanism in Alzheimer's disease,in which histone deacetylase 6 inhibition by valproic acid increases V-ATPase assembly and lysosomal acidification.

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Burden of six major types of digestive system cancers globally and in China

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中华医学杂志(英文版) 2024年第16期137卷 1957-1964页

作者： Zhou yueyang Song Kai Chen Yuqing Zhang Yuelun Dai Min Wu dong Chen Hongda Center for Prevention and Early Intervention National Infrastructures for Translational Medicine Institute of Clinical Medicine Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing China Department of Gastroenterology Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing China Department of Cancer Epidemiology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Department of Gastroenterology People’s Hospital of Xizang Autonomous Region Lhasa Xizang autonomous region China

Background: Digestive system cancers constitute a significant number of cancer cases, but their burden is not uniform. As Global Cancer Observatory (GLOBOCAN) 2022 has recently updated its estimates of cancer burden, we aimed to investigate the burden of six major digestive system cancers both worldwide and in China, along with geographical and temporal variations in cancer-specific incidence and ***: We extracted data on primary cancers of the esophagus, stomach, colorectum, liver, pancreas, and gallbladder from the GLOBOCAN database for 2022. Age-standardized incidence and mortality rates were calculated and stratified by sex, country, region, and human development index (HDI). We used the 2022 revision of the World Population Prospects (United Nations) to obtain demographic data for various age groups in China from 1988 to 2012 and used the joinpoint model and the average annual percentage change (AAPC) to analyze cancer incidence trends in ***: In 2022, the estimated global incidence of digestive system cancers reached 4,905,882, with an estimated 3,324,774 cancer-related deaths. Colorectal cancer was most prevalent in terms of incidence and mortality. There was a significant correlation between the burden of gastrointestinal cancers and country HDI. From 1988 to 2012, the incidence of esophageal, gastric, and liver cancers declined in China, whereas colorectal and pancreatic cancer incidences continued to increase. By 2050, colorectal and liver cancers are projected to remain the leading cancer types in China in terms of incidence and mortality, ***: Digestive system cancers remain a significant public health challenge globally and in China. Although progress has been made in the prevention and control of some cancers, the burden of digestive system cancers persists. The implementation of tertiary prevention strategies must be intensified to reduce the incidence and mortality of digestive system cancers, mitigating t

关键词： Digestive system cancer Incidence Mortality GLOBOCAN 2022 China Global Esophagus cancer Stomach cancer Colorectal cancer

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高度城镇化地区河网水系生态调控方案

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水资源保护 2022年第1期38卷 205-212页

作者：王鹏华祖林褚克坚董越洋河海大学浅水湖泊综合治理与资源开发教育部重点实验室江苏南京210098 河海大学长江保护与绿色发展研究院江苏南京210098

为探究长三角高度城镇化地区河网水系生态调控方案,以秦淮河流域下游地区水系为例,采用生态功能设定法,计算了各河段生态水位和生态流量。通过构建一维水量水质模型,在枯水年非汛期水量调控及截污措施协同驱动下,对水位、流量及污染物... 详细信息

为探究长三角高度城镇化地区河网水系生态调控方案,以秦淮河流域下游地区水系为例,采用生态功能设定法,计算了各河段生态水位和生态流量。通过构建一维水量水质模型,在枯水年非汛期水量调控及截污措施协同驱动下,对水位、流量及污染物浓度的时空分布特征进行多情景数值模拟,并对生态水量保障率及水质改善效果进行评估。结果表明,水位调控措施对水质改善作用非常有限,无法保障河道生态基流需求;引水措施的调控效果较为明显,但水质改善率增幅随引水流量增加而下降;截污措施的水质改善效果优于水量调控方案,但存在显著的空间异质性,且无法提高生态基流保障率;将截污措施与引水措施结合,是满足城市水系生态水量及水质要求的最佳选择。

关键词：高度城镇化生态需水量生态水位多目标调控水量水质模型秦淮河流域

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重组抗菌肽的表达及其在饲料中的应用研究进展

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中国畜牧杂志 2023年第12期59卷 67-73,79页

作者：司先懂姜宏董悦阳董格菲陈鹏毛相朝中国海洋大学食品科学与工程学院山东青岛266000 北京英惠尔生物技术有限公司北京100081

抗菌肽是广泛存在于生物体内的一种小分子多肽类物质,具有抗革兰氏阴性菌和革兰氏阳性菌的作用,有抗菌谱广、不易分解、稳定性好且不易产生抗药性等特点。抗菌肽作为动物饲料中抗生素的理想替代品,逐渐受到行业的重视。抗菌肽的异源表... 详细信息

抗菌肽是广泛存在于生物体内的一种小分子多肽类物质,具有抗革兰氏阴性菌和革兰氏阳性菌的作用,有抗菌谱广、不易分解、稳定性好且不易产生抗药性等特点。抗菌肽作为动物饲料中抗生素的理想替代品,逐渐受到行业的重视。抗菌肽的异源表达可降低抗菌肽制剂的生产成本,促进抗菌肽制剂的规模化应用。本文对重组抗菌肽的来源、作用机理、重组表达及其在动物养殖中的应用等方面进行综述,为抗菌肽在饲料中的应用提供参考。

关键词：抗菌肽重组表达饲料

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Method for triangular fuzzy multiple attribute decision making based on two-dimensional density operator method

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Journal of Systems Engineering and Electronics 2024年第1期35卷 178-185页

作者： LIN Youliang LI Wu LIU Gang HUANG dong School of Economics and Management Hunan Institute of Science and TechnologyYueyang 414000China Hunan Vocational College for Nationalities Yueyang 414000China School of Information Science and Engineering Hunan Institute of Science and TechnologyYueyang 414000China

Aiming at the triangular fuzzy(TF)multi-attribute decision making(MADM)problem with a preference for the distribution density of attribute(DDA),a decision making method with TF number two-dimensional density(TFTD)operator is proposed based on the density operator theory for the decision maker(DM).Firstly,a simple TF vector clustering method is proposed,which considers the feature of TF number and the geometric distance of ***,the least deviation sum of squares method is used in the program model to obtain the density weight ***,two TFTD operators are defined,and the MADM method based on the TFTD operator is ***,a numerical example is given to illustrate the superiority of this method,which can not only solve the TF MADM problem with a preference for the DDA but also help the DM make an overall comparison.

关键词： fuzzy decision making clustering density operator multi-attribute decision making(MADM)

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辣椒叶片水分胁迫高光谱特性研究

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农业机械学报 2024年第7期55卷 336-344页

作者：王东孙欣张岳阳夏鹤宁逯明辉周林凡西北农林科技大学机械与电子工程学院杨凌712100 农业农村部农业物联网重点实验室杨凌712100 西北农林科技大学园艺学院杨凌712100

为精准判别作物需水程度,以生长期辣椒为实验样本,对辣椒进行不同程度的水浸、干旱等水分胁迫处理,分析不同水分胁迫程度下辣椒叶片的高光谱响应特性。样本分为重度干旱、轻度干旱、轻度水浸、重度水浸等4个水分胁迫组和一个实验对照组,... 详细信息

为精准判别作物需水程度,以生长期辣椒为实验样本,对辣椒进行不同程度的水浸、干旱等水分胁迫处理,分析不同水分胁迫程度下辣椒叶片的高光谱响应特性。样本分为重度干旱、轻度干旱、轻度水浸、重度水浸等4个水分胁迫组和一个实验对照组,共5个数据组,每组20株辣椒,待各组叶片外观出现明显差异时,分别采集各组辣椒叶片的叶绿素荧光参数与高光谱数据。比较多元散射校正(MSC)、SG卷积平滑滤波和标准正态变换(SNV)3种不同的预处理方法对背景信息干扰的消除效果。采用SPA算法和CARS算法提取对水分胁迫敏感的特征波长。建立预测辣椒叶片不同水分胁迫程度的支持向量机(SVM)、BP神经网络、径向基函数(RBF)和随机森林(RF)模型。结果说明,SG-SPA-RFB为预测辣椒叶片水分胁迫程度的最优组合,其训练集准确率为99.02%,测试集准确率为94.00%。本研究为判断辣椒植株水分胁迫状态提供了一种便捷可靠的无损检测方法。

关键词：辣椒水分胁迫高光谱图像特征波长神经网络叶绿素荧光参数

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