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Microarray analysis of Escherichia coli0157:H7

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World Journal of Gastroenterology 2005年第37期11卷 5811-5815页

作者： Hui-Ying Jin Kai-Hua Tao yue-xi li Fa-Qing li Su-Qin li Huadong Research Institute for Medicine and Biotechnics Nanjing 210002 Jiangsu Province China

AIM： To establish the rapid, specific, and sensitive method for detecting O157：H7 with DNA microchips. METHODS： Specific oligonucleotide probes （26-28 nt） of bacterial antigenic and virulent genes of E. coliO157：H7 and other related pathogen genes were pre-synthesized and immobilized on a solid support to make microchips. The four genes encoding O157 somatic antigen （rfbE）, H7 flagellar antigen （fliC） and toxins （SLT1, SLT2） were monitored by multiplex PCR with four pairs of specific primers. Fluorescence-Cy3 labeled samples for hybridization were generated by PCR with Cy3-1abeled single prime. Hybridization was performed for 60 min at 45 ℃. Microchip images were taken using a confocal fluorescent scanner. RESULTS： Twelve different bacterial strains were detected with various combinations of four virulent genes. All the O157：H7 strains yielded positive results by multiplex PCR. The size of the PCR products generated with these primers varied from 210 to 678 bp. All the rfbE/fliC/SLT1/SLT2 probes specifically recognized Cy3-1abeled fluorescent samples from O157：H7 strains, or strains containing O157 and H7 genes. No cross hybridization of O157：H7 fluorescent samples occurred in other probes. Non-O157：H7 pathogens failed to yield any signal under comparable conditions. If the Cy3-1abeled fluorescent product of O157 single PCR was diluted 50-fold, no signal was found in agarose gel electrophoresis, but a positive signal was found in microarray hybridization. CONCLUSION： Microarray analysis of O157：H7 is a rapid, specific, and efficient method for identification and detection of bacterial pathogens.

关键词： Microarray Multiplex PCR, Escherichia coli O157:H7 Shiga-like toxin

Correlation of Helicobacter pylori infection with high-density lipoprotein cholesterol levels and pulse wave conduction velocity

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Clinical Research Communications 2024年第4期7卷 33-38页

作者： yue-xi li xiao-Qin liu Yu-Han Luo Qiao-li Wang Health Management Center Deyang People’s HospitalDeyang 618000China

Background:Helicobacter pylori(HP)is associated with several gastrointestinal diseases,including peptic ulcer diseases and gastric cancer,and non-gastrointestinal diseases such as hypertension and Alzheimer's ***,the relationship between HP and lipid metabolism and atherosclerosis remains *** study aims to investigate the association between *** infection and high-density lipoprotein cholesterol levels and pulse wave conduction ***:This is a report of a cross-sectional study that collected data from 2,827 *** data collected included results of life questionnaires,laboratory tests,13C-urea breath test(13C-UBT),and pulse wave conduction velocity *** on the results of the 13C-UBT test,the subjects were divided into two groups:the HP-uninfected group(HP−)and the HP-infected group(HP+).The study compared the differences in HDL-C levels and brachial-ankle pulse wave velocity(baPWV)between the two ***-way regression analysis was used to identify potential factors affecting HDL-C levels in the study *** regression equations were presented to analyze whether HP infection was an independent risk factor for abnormal HDL-C metabolism in the ***:Univariate analysis demonstrated that high-density lipoprotein cholesterol(HDL-C)levels were significantly lower in the HP+group compared to the HP−group,with a mean difference ofβ=−18.1 mg/dl(95%CI:−19.3 to−17.0,P<0.001).After adjusting for all variables,the HDL-C levels remained lower in the HP+group compared to the HP-group,with a mean difference ofβ=−17.4 mg/dl(95%CI:−18.2 to−16.7,P<0.001).These findings suggest that *** infection is independently associated with abnormal HDL-C ***,brachial-ankle pulse wave velocity(baPWV)was higher in the HP+group than in the HP−group on both *** the right side,the baPWV was 1,713.4±231.4 cm/s in the HP+group compared to 1,542.8±237.5 cm/s in the HP−group(t=−18.30,P<0.001).O

关键词： Helicobacter pylori cholesterol high-density lipoprotein atherosclerosis

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双轴张应变对锗激光器工作性能的影响

华南理工大学学报（自然科学版） 2017年第7期45卷 120-125页

作者：李希越李斌 xiA Guangrui 华南理工大学电子与信息学院广东广州510640 英属哥伦比亚大学材料工程系

为探索锗激光器的性能优化方案,建立了基于双轴张应变的双异质结法布里-珀罗电激励式边缘发射锗激光器模型.通过该模型讨论了双轴张应变与最优掺杂密度的关系,分析了不同双轴张应变和掺杂条件下阈值电流密度、电光转换效率等激光器参数... 详细信息

为探索锗激光器的性能优化方案,建立了基于双轴张应变的双异质结法布里-珀罗电激励式边缘发射锗激光器模型.通过该模型讨论了双轴张应变与最优掺杂密度的关系,分析了不同双轴张应变和掺杂条件下阈值电流密度、电光转换效率等激光器参数的变化.结果表明:最优掺杂密度随着双轴张应变的增大而减小,过高的掺杂则导致激光器工作性能的下降;在同等掺杂条件下,光增益以及增益的峰值波长会随着双轴张应变的增大而增大;与文献数据相比,在0.8%双轴张应变和对应的最优掺杂密度(8×1019cm-3)下,锗激光器的阈值电流密度降低至文献数据的1/10,电光转换效率提升了约10倍.

关键词：锗激光器双轴张应变最优掺杂密度光增益阈值电流密度电光转换效率

三黄糖肾康颗粒对早期糖尿病肾病模型大鼠微炎症状态的影响

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中国全科医学 2017年第30期20卷 3770-3775页

作者：奚悦李敬林锦州医科大学附属第三医院辽宁省锦州市121000 辽宁中医药大学辽宁省沈阳市110032

目的探讨三黄糖肾康颗粒对早期糖尿病肾病模型大鼠微炎症状态的影响。方法研究时间为2012年8—10月,SPF级Wistar大鼠90只,雌雄各半,随机选取10只作为正常对照组,给予常规颗粒饲料喂养;其余80只随机分为模型组、西药组、中药预防组、中... 详细信息

目的探讨三黄糖肾康颗粒对早期糖尿病肾病模型大鼠微炎症状态的影响。方法研究时间为2012年8—10月,SPF级Wistar大鼠90只,雌雄各半,随机选取10只作为正常对照组,给予常规颗粒饲料喂养;其余80只随机分为模型组、西药组、中药预防组、中药低剂量组、中药高剂量组,各16只,均给予高脂高糖饲料喂养。1周后除正常对照组大鼠外其余各组大鼠均予以链脲佐菌素(STZ)溶液42 mg/kg一次性腹腔注射,诱导糖尿病模型,8只大鼠造模失败;余72只大鼠2周后收集24 h尿液检测尿微量清蛋白(MALB)以判定早期糖尿病肾病模型是否成功,其中6只大鼠糖尿病肾病模型造模失败。实验过程中,模型组3只大鼠死亡,西药组1只大鼠死亡,中药低剂量组、中药高剂量组各有2只大鼠死亡。正常对照组和模型组均给予蒸馏水灌胃,中药预防组大鼠自早期糖尿病肾病造模成功日起即开始给药,连续给药10周;其余各组造模成功2周后,分别给予相应药物灌胃,连续给药8周。西药组给予贝那普利灌胃,中药高剂量组使用浓度为3.84 g/ml的三黄糖肾康颗粒药液,中药预防组和中药低剂量组使用浓度为1.28 g/ml的三黄糖肾康颗粒药液。实验结束后测定大鼠血糖(FBG)、糖化血红蛋白(HbA_(1c))、MALB、血清β2-微球蛋白(β2-MG)、血肌酐(Scr)、尿素氮(BUN)、血清及肾脏组织中C反应蛋白(CRP),肾脏组织中核因子κB(NF-κB)、单核细胞趋化蛋白-1(MCP-1)、细胞表面趋化因子受体2(CCR2)mRNA及蛋白表达水平,光镜下观察肾脏组织形态变化(HE染色和PAS染色)。结果模型组、西药组、中药预防组、中药低剂量组、中药高剂量组FBG、HbA_(1c)、MALB高于正常对照组(P<0.01);西药组、中药预防组、中药低剂量组、中药高剂量组FBG、HbA_(1c)、MALB、血清及肾脏组织中CRP,肾脏组织中NF-κB、MCP-1、CCR2 mRNA及蛋白表达水平均低于模型组(P<0.01);中药预防组FBG、MALB低于西药组、中药低剂量组、中药高剂量组(P<0.05)。正常对照组、西药组、中药预防组、中药低剂量组、中药高剂量组大鼠BUN低于模型组(P<0.01);中药低剂量组大鼠BUN高于正常对照组(P<0.05)。模型组大鼠血清及肾脏组织中CRP,肾脏组织中NF-κB、MCP-1、CCR2 mRNA及蛋白表达水平均高于正常对照组(P<0.01)。中药低剂量组、中药高剂量组大鼠肾脏组织中NF-κB mRNA及蛋白表达水平均高于中药预防组(P<0.01)。西药组大鼠肾脏组织中NF-κB mRNA及蛋白表达水平高于中药预防组、而低于中药低剂量组(P<0.01)。中药预防组大鼠肾脏组织中MCP-1、CCR2 mRNA及蛋白表达水平低于西药组及中药低、高剂量组(P<0.05);西药组MCP-1、CCR2 mRNA及蛋白表达水平低于中药低、高剂量组(P<0.05)。西药组、中药预防组、中药低剂量组、中药高剂量组大鼠肾脏病理变化程度明显轻于模型组。结论三黄糖肾康颗粒可有效降低糖尿病肾病模型大鼠FBG、HbA_(1c)、MALB、BUN、血清及肾脏组织中CRP,下调肾脏组织NF-κB、MCP-1、CCR2 mRNA及蛋白表达水平,改善肾脏形态学病变,减轻糖尿病肾病微炎症状态。

关键词：糖尿病肾病大鼠炎症三黄糖肾康

Targets capture by distributed active swarms via bio-inspired reinforcement learning

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 210-221页

作者： Kun Xu yue li Jun Sun Shuyuan Du xinpeng Di Yuguang Yang Bo li Institute of Biomechanics and Medical Engineering Applied Mechanics Laboratory Department of Engineering MechanicsTsinghua University Institute of Nuclear and New Energy Technology Tsinghua University Shanghai Aerospace Control Technology Institute Shanghai Key Laboratory of Aerospace Intelligent Control Technology College of Automation Engineering Nanjing University of Aeronautics and Astronautics

Natural swarms arranged from cells to herds are usually decentralized but display intriguing collective intelligence in coordinating individuals across large scales to efficiently achieve their common goals. Learning from nature may provide new strategies for controlling collective dynamics of synthetic swarms to accomplish specific functions. Here, we present a bioinspired computational framework that steers distributed active swarms to collectively capture and merge targets via reinforcement learning. We exploit collective milling structures of natural herds to cage the targets, and adopt a switching control policy inspired by sperms' chiral dynamics to optimize the trajectories of individuals, through which the active swarms can selforganize to enclose single or multiple distant targets in a dynamical, adaptive and scalable manner. There exists a critical swarm size, beyond which the excessive competition between agents would generate large mechanical forces, leading to capture instability but enabling the transition from short-distance to long-distance merging capture of multiple targets. This work provides physical insights into distributed active swarms and could offer a multilevel, decentralized strategy toward controlling swarm robotics in wide applications such as bio-medical devices, machine immunity, and target clearance.

关键词： swarm intelligence robotics multi-tasks artificial intelligence bionics

A Rapid Adaptation Approach for Dynamic Air‑Writing Recognition Using Wearable Wristbands with Self‑Supervised Contrastive Learning

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Nano-Micro Letters 2025年第2期17卷 417-431页

作者： Yunjian Guo Kunpeng li Wei yue Nam‑Young Kim Yang li Guozhen Shen Jong‑Chul Lee Department of Electronic Convergence Engineering Kwangwoon UniversitySeoul 01897South Korea Radio Frequency Integrated Circuit(RFIC)Bio Centre Kwangwoon UniversitySeoul 01897South Korea Department of Electronic Engineering Kwangwoon UniversitySeoul 01897South Korea School of Microelectronics Shandong UniversityJinan 250101People’s Republic of China State Key Laboratory of Integrated Chips and Systems Fudan UniversityShanghai 200433People’s Republic of China School of Integrated Circuits and Electronics Beijing Institute of TechnologyBeijing 100081People’s Republic of China

Wearable wristband systems leverage deep learning to revolutionize hand gesture recognition in daily *** existing approaches that often focus on static gestures and require extensive labeled data,the proposed wearable wristband with selfsupervised contrastive learning excels at dynamic motion tracking and adapts rapidly across multiple *** features a four-channel sensing array composed of an ionic hydrogel with hierarchical microcone structures and ultrathin flexible electrodes,resulting in high-sensitivity capacitance *** wireless transmission from a Wi-Fi module,the proposed algorithm learns latent features from the unlabeled signals of random wrist ***,only few-shot labeled data are sufficient for fine-tuning the model,enabling rapid adaptation to various *** system achieves a high accuracy of 94.9%in different scenarios,including the prediction of eight-direction commands,and air-writing of all numbers and *** proposed method facilitates smooth transitions between multiple tasks without the need for modifying the structure or undergoing extensive task-specific *** utility has been further extended to enhance human–machine interaction over digital platforms,such as game controls,calculators,and three-language login systems,offering users a natural and intuitive way of communication.

关键词： Wearable wristband Self-supervised contrastive learning Dynamic gesture Air-writing Human-machine interaction

FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression

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Neural Regeneration Research 2025年第7期20卷 2068-2083页

作者： Jing Yao Yuan li xi liu Wenping liang Yu li liyong Wu Zhe Wang Weihong Song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Pediatrics The Second Affiliated Hospital and Yuying Children’s HospitalKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine Visionand Brain Health)WenzhouZhejiang ProvinceChina

Alzheimer's disease is characterized by deposition of amyloid-β,which forms extracellular neuritic plaques,and accumulation of hyperphosphorylated tau,which aggregates to form intraneuronal neurofibrillary tangles,in the *** NLRP3 inflammasome may play a role in the transition from amyloid-βdeposition to tau phosphorylation and *** NLRP3 is primarily found in brain microglia,and tau is predominantly located in neurons,it has been suggested that NLRP3 expressed by microglia indirectly triggers tau phosphorylation by upregulating the expression of pro-inflammatory ***,we found that neurons also express NLRP3 in vitro and in vivo,and that neuronal NLRP3 regulates tau *** biochemical methods,we mapped the minimal NLRP3 promoter and identified FUBP3 as a transcription factor regulating NLRP3 expression in *** primary neurons and the neuroblastoma cell line Neuro2A,FUBP3 is required for endogenous NLRP3 expression and tau phosphorylation only when amyloid-βis *** the brains of aged wild-type mice and a mouse model of Alzheimer's disease,FUBP3 expression was markedly increased in cortical *** analysis suggested that FUBP3 plays a role in neuron-mediated immune *** also found that FUBP3 trimmed the 5′end of DNA fragments that it bound,implying that FUBP3 functions in stress-induced *** findings suggest that neuronal NLRP3 may be more directly involved in the amyloid-β-to–phospho-tau transition than microglial NLRP3,and that amyloid-βfundamentally alters the regulatory mechanism of NLRP3 expression in *** that FUBP3 was only expressed at low levels in young wild-type mice and was strongly upregulated in the brains of aged mice and Alzheimer's disease mice,FUBP3 could be a safe therapeutic target for preventing Alzheimer's disease progression.

关键词： 5′end trimming Alzheimer's disease amyloid-beta amyloid-β-dependent transcription FUBP3 inflammasome inflammation neuron NLRP3 tau transcription factor

Hypidone hydrochloride(YL-0919)ameliorates functional deficits after traumatic brain injury in mice by activating the sigma-1 receptor for antioxidation

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Neural Regeneration Research 2025年第8期20卷 2325-2336页

作者： Yafan Bai Hui Ma yue Zhang Jinfeng li xiaojuan Hou Yixin Yang Guyan Wang Yunfeng li Department of Anesthesiology Beijing Tongren HospitalCapital Medical UniversityBeijingChina Beijing Institute of Basic Medical Sciences BeijingChina Hebei North University ZhangjiakouHebei ProvinceChina Beijing Institute of Pharmacology and Toxicology State Key Laboratory of Toxicology and Medical CountermeasuresBeijing Key Laboratory of NeuropsychopharmacologyBeijingChina

Traumatic brain injury involves complex pathophysiological mechanisms,among which oxidative stress significantly contributes to the occurrence of secondary *** this study,we evaluated hypidone hydrochloride(YL-0919),a self-developed antidepressant with selective sigma-1 receptor agonist properties,and its associated mechanisms and targets in traumatic brain *** experiments to assess functional deficits were followed by assessment of neuronal damage through histological analyses and examination of blood-brain barrier permeability and brain ***,we investigated the antioxidative effects of YL-0919 by assessing the levels of traditional markers of oxidative stress in vivo in mice and in vitro in HT22 ***,the targeted action of YL-0919 was verified by employing a sigma-1 receptor antagonist(BD-1047).Our findings demonstrated that YL-0919 markedly improved deficits in motor function and spatial cognition on day 3 post traumatic brain injury,while also decreasing neuronal mortality and reversing blood-brain barrier disruption and brain ***,YL-0919 effectively combated oxidative stress both in vivo and in *** protective effects of YL-0919 were partially inhibited by *** results indicated that YL-0919 relieved impairments in motor and spatial cognition by restraining oxidative stress,a neuroprotective effect that was partially reversed by the sigma-1 receptor antagonist ***-0919 may have potential as a new treatment for traumatic brain injury.

关键词： antidepressant drug blood-brain barrier cognitive function hypidone hydrochloride(YL-0919) neurological function nuclear factor-erythroid 2 related factor 2 oxidative stress sigma-1 receptor superoxide dismutase traumatic brain injury

“One stone, two birds”: Salt template enabling porosity engineering and single metal atom coordinating toward high-performance zinc-ion capacitors

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Journal of Energy Chemistry 2025年第1期100卷 637-645页

作者： Chunliu Zhu Huanyu liang Ping li Chenglong Qiu Jingyi Wu Jingwei Chen Weiqian Tian yue Zhu Zhi li Huanlei Wang School of Materials Science and Engineering Ocean University of China Department of Chemical and Materials Engineering University of Alberta

Zinc-ion hybrid capacitors （ZIHCs） have received increasing attention as energy storage devices owing to their low cost,high safety,and environmental ***,their progress has been hampered by low energy and power density,as well as unsatisfactory long-cycle stability,mainly due to the lack of suitable electrode *** this context,we have developed manganese single atoms implanted in nitrogen-doped porous carbon nanosheets （MnSAs/NCNs） using a metal salt template method as cathodes for *** metal salt serves a dual purpose in the synthesis process:It facilitates the uniform dispersion of Mn atoms within the carbon matrix and acts as an activating agent to create the porous *** applied in ZIHCs,the MnSAs/NCNs electrode demonstrates exceptional performance,including a high capacity of 203 m Ah g-1,an energy density of 138 Wh kg-1at 68 W kg-1,and excellent cycle stability with 91%retention over 10,000 *** calculations indicate that the introduced Mn atoms modulate the local charge distribution of carbon materials,thereby improving the electrochemical *** work demonstrates the significant potential of carbon materials with metal atoms in zinc-ion hybrid capacitors,not only in enhancing electrochemical performance but also in providing new insights and methods for developing high-performance energy storage devices.

关键词： Zinc ion hybrid capacitors Cathodes Carbon materials Heteroatoms Single atoms