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Copper-Free Click Chemistry-Mediated Assembly of Single Quantum Dot Nanosensor for Accurately Monitoring Locus-Specific m^(6)A in Cancer Cells

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Chinese Journal of Chemistry 2024年第5期42卷 499-504页

作者： yue-ying li Ning-Ning Zhao Yi-Xuan Geng Qian Han Jian-Ge Qiu Bing-Hua Jiang Zi-yue Wang Chun-Yang Zhang Translational Medicine Center The First Affiliated Hospital of Zhengzhou UniversityThe Academy of Medical SciencesHenan Institute of Medical and Pharmaceutical Sciences BGI CollegeZhengzhou UniversityZhengzhouHenan450052 China College of Chemistry Chemical Engineering and Materials ScienceShandong Normal UniversityJinanShandong250014 China School of Chemistry and Chemical Engineering Southeast UniversityNanjingJiangsu211189 China

N6-methyladenosine(m^(6)A)plays an important role in embryogenesis,nuclear export,transcription splicing,and protein translation ***,we demonstrate a copper-free click chemistry-mediated assembly of single quantum dot(QD)nanosensor for accurately monitoring locus-specific m^(6)A in cancer *** m^(6)A-sensitive endoribonuclease MazF can digest the unmethylated A-RNA,and the intact m^(6)A-RNA then hybridizes with DNA probes a and b to produce a sandwich hybrid,initiating the click chemistry to generate probe a–b ligation product via first tandem ligation detection reaction(LDR)***,DNA probes c and d can hybridize with the probe a–b ligation product to generate the probe c–d ligation product via second LDR *** LDR cycles can be repeated through denaturation and annealing reaction to generate abundant biotin-/fluorophore-modified probe c–d ligation products that can easily assemble on the QD surface to induce distinct fluorescence resonance energy transfer(FRET)between QD and *** assay can be homogenously performed without the involvement of copper catalyst,m^(6)A-specific antibody,radioactive labeling,ligase enzyme,enzymatic reverse transcription,and next-generation ***,it can discriminate even 0.01% m^(6)A level in complex samples and accurately measure cellular m^(6)A-RNA expression,providing a promising avenue for clinical diagnostics and biomedical research.

关键词： RNA methylation Click chemistry Förster resonance energy transfer Biosensor Cancer

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High dimensional proteomic mapping of bone marrow immune characteristics in immune thrombocytopenia

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Science China(life Sciences) 2024年第8期67卷 1635-1647页

作者： Feng-Qi liu Qing-Yuan Qu ying Lei Qi Chen Yu-Xiu Chen Meng-lin li Xue-Yan Sun Ye-Jun Wu Qiu-Sha Huang Hai-Xia Fu Yuan Kong yue-ying li Qian-Fei Wang Xiao-Jun Huang Xiao-Hui Zhang Peking University People’s Hospital Peking University Institute of HematologyBeijing 100044China Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Beijing 100044China National Clinical Research Center for Hematologic Disease Beijing 100044China Collaborative Innovation Centre of Hematology Peking UniversityBeijing 100044China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Peking-Tsinghua Center for life Sciences Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100074China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China

To investigate the role of co-stimulatory and co-inhibitory molecules on immune tolerance in immune thrombocytopenia(ITP),this study mapped the immune cell heterogeneity in the bone marrow of ITP at the single-cell level using Cytometry by Time of Flight(CyTOF).Thirtysix patients with ITP and nine healthy volunteers were enrolled in the *** soluble immunomodulatory molecules,more sCD25 and sGalectin-9 were detected in ITP *** the cell surface,co-stimulatory molecules like ICOS and HVEM were observed to be upregulated in mainly central memory and effector T *** contrast,co-inhibitory molecules such as CTLA-4 were significantly reduced in Th1 and Th17 cell *** a platelet count of 30×10^(9)L^(−1)as the cutoff value,ITP patients with high and low platelet counts showed different T cell immune ***-presenting cells such as monocytes and B cells may regulate the activation of T cells through CTLA-4/CD86 and HVEM/BTLA interactions,respectively,and participate in the pathogenesis of *** conclusion,the proteomic and soluble molecular profiles brought insight into the interaction and modulation of immune cells in the bone marrow of *** may offer novel targets to develop personalized immunotherapies.

关键词： immune thrombocytopenia autoimmune diseases co-stimulatory co-inhibitory immune checkpoint CTLA4 Cytometry by Time of Flight bone marrow

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基于蝴蝶优化算法的通信传输链路抗干扰仿真

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计算机仿真 2024年第2期41卷 401-405页

作者：陈瑾瑾岳丽颖郑州工业应用技术学院信息工程学院河南郑州451150 深圳大学电子与信息工程学院广东深圳518000

为了避免干扰信号影响信息在链路中的传输效果,提出可见光通信高速传输链路抗干扰方法。建立可见光通信网络模型,对链路抗干扰问题建模;针对上述模型建立可见光通信高速传输链路抗干扰优化目标函数,并选取信道容量、信道误码率和链路中... 详细信息

为了避免干扰信号影响信息在链路中的传输效果,提出可见光通信高速传输链路抗干扰方法。建立可见光通信网络模型,对链路抗干扰问题建模;针对上述模型建立可见光通信高速传输链路抗干扰优化目标函数,并选取信道容量、信道误码率和链路中断概率作为约束条件;引入蝴蝶优化算法求解目标函数,实现可见光通信高速传输链路抗干扰。实验结果表明,所提方法的误码率低、信息传输成功率高、抗干扰能力强,能够确保通信传输的顺畅性,具有实际应用价值。

关键词：可见光通信技术链路抗干扰优化误码率链路中断概率蝴蝶优化算法

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LncRNA CRNDE调节miR⁃338⁃3p/KLF6轴对LPS诱导的肺泡上皮细胞损伤的影响

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解剖学研究 2024年第3期46卷 228-235页

作者：岳利英林雪容张盟盟张家口市第一医院输血科河北张家口075000 河北北方学院附属第一医院急诊科河北张家口075000

目的探讨LncRNA结直肠肿瘤差异表达基因(CRNDE)调节miR⁃338⁃3p/KLF6轴对LPS诱导的肺泡上皮细胞损伤的影响。方法将人肺泡上皮细胞A549分为CK组、LPS组、LPS+si⁃NC组、LPS+si⁃CRNDE组、LPS+si⁃CRNDE+inhibitor NC组、LPS+si⁃CRNDE+miR⁃338⁃3p inhibitor组、LPS+si⁃CRNDE+oe⁃NC组、LPS+si⁃CRNDE+oe⁃KLF6组;qRT⁃PCR检测各组细胞CRNDE、miR⁃338⁃3p和KLF6表达水平;MTT法检测细胞增殖;流式细胞术检测细胞凋亡率;EliSA试剂盒检测TNF⁃α、IL⁃10和IL⁃1β水平;商品化试剂盒分析MDA、GSH⁃Px、SOD水平;Western blot检测细胞中KLF6、Cleaved⁃caspase⁃3、Bax、Bcl⁃2蛋白表达量。双荧光素酶报告基因实验验证miR⁃338⁃3p与RNDE和KLF6的关系。结果与CK组相比,LPS组和LPS+si⁃NC组A549细胞CRNDE和KLF6 mRNA表达、TNF⁃α、IL⁃1β水平、MDA含量、细胞凋亡率、caspase⁃3、Bax、KLF6蛋白表达显著升高,miR⁃338⁃3p表达、细胞活力、IL⁃10水平、SOD和GSH⁃Px活性、Bcl⁃2蛋白表达显著降低(P<0.05);与LPS+si⁃NC组相比,LPS+si⁃CRNDE组A549细胞CRNDE和KLF6 mRNA表达、TNF⁃α、IL⁃1β水平、MDA含量、细胞凋亡率、caspase⁃3、Bax、KLF6蛋白表达显著降低,miR⁃150⁃5p表达、细胞活力、IL⁃10水平、SOD和GSH⁃Px活性、Bcl⁃2蛋白表达显著升高(P<0.05);干扰miR⁃338⁃3p表达或过表达KLF6均可降低下调CRNDE表达改善LPS诱导A549细胞损伤的作用(P<0.05)。结论下调CRNDE可调控miR⁃338⁃3p/KLF6轴减轻LPS诱导的A549细胞损伤。

关键词：急性肺损伤长链非编码RNA 结直肠肿瘤差异表达基因 miR⁃338⁃3p/KLF6轴脂多糖肺泡上皮细胞

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Well-dispersed Sb particles embedded on N-doped carbon nanofibers toward high-performance li-ion battery

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Rare Metals 2024年第7期43卷 2994-3006页

作者： ying li Yi-Han Zhao Lu-Lu Zhao Peng-Fei Wang ying Xie Ting-Feng Yi School of Materials Science and Engineering Northeastern UniversityShenyang 110819China Key Laboratory of Dielectric and Electrolyte Functional Material Hebei Province School of Resources and MaterialsNortheastern University at QinhuangdaoQinhuangdao 066004China Key Laboratory of Functional Inorganic Material Chemistry Ministry of EducationSchool of Chemistry and Materials ScienceHeilongjiang UniversityHarbin 150080China

The alloy-type material Sb is widely used in the anode materials of lithium-ion batteries(liBs)due to its high theoretical specific ***,its serious volume expansion problem during alloying/dealloying of li~+limits its practical *** this work,C-Sb composite was constructed as anode material of liBs by electrospinning route for the first time,Sb was introduced into the polyacrylonitrile-based hard carbon and coal tar pitch-based soft carbon composite amorphous carbon fiber with a diameter of 300-600 nm,which realized high cycling *** C-Sb-2(the mass ratio of polyacrylonitrile to Sb source is 1:2)electrode displayed charge capacities of 1098.5,930.3,841.7,753.5,643.9 and 545.8 mAh·g^(-1)at 0.1,0.2,0.3,0.5,1 and 2 A·g^(-1),*** when the current density returned to 0.1 A·g^(-1),the charge capacity was 939.3 mAh·g^(-1),revealing good stability and *** introduction of Sb into the amorphous carbon improved its conductivity and addressed the volume expansion issue of high specific capacity Sb during charge/***-situ XRD analysis confirmed the high reversibility of the C-Sb-2 during charging and *** functional theory(DFT)calculations revealed the gradual enhancement of the interface interaction between Sb_(x)li_(y) and amorphous carbon(AMC)with increasing lithium content,contributing to the anchoring of alloy nanoparticles on the AMC surface and buffering the volume change of the ***,the gradual lithiation of Sb facilitated the electron transfer from Sb_(x)li_(y) to *** findings hold promise for designing lithium storage materials with exceptional performance,highlighting the potential of C-Sb composites as anode materials for efficient next-generation lithium storage.

关键词： li-ion battery Metallic Sb Carbon Electrospinning Cycling stability

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li_(2)ZnTi_(3)O_(8)as the host–separator modifier with efficient polysulfides trapping and fast li^(+)diffusion for lithium-sulfur batteries

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Nano Research 2024年第7期17卷 6087-6094页

作者： Mao Qian Yakun Tang Lang liu yue Zhang Xiaohui li JiaJia Chen State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources Xinjiang UniversityUrumqi 830017China State Key Laboratory of Physical Chemistry of Solid Surfaces Xiamen UniversityXiamen 361005China

The diffusion and loss of lithium polysulfides(liPSs)in lithium-sulfur batteries(LSBs)reduce the sulfur utilization rate and the catalytic conversion efficiency of sulfur species,resulting in early battery ***_(2)ZnTi_(3)O_(8)(LZTO),characterized by its stable spinel structure,exhibits high li+conductivity and holds great potential as an effective adsorbent for *** study proposes a collaborative design concept of LZTO host–separator modifier,which offers a complementary and matching approach in the cathode side,effectively addressing the challenges associated with dissolution and inadequate conversion of *** functional theory(DFT)calculation substantiates the pronounced chemical affinity of LZTO towards *** importantly,the high efficiency ion transport channels are achieved in separator coating due to the presence of the LZTO ***,the catalytic efficacy of LZTO is validated through meticulous analysis of symmetric batteries and Tafel ***,the LZTO host–separator modifier-based cell displays satisfactory rate capability(1449 and 1166 mAh·g^(−1)at 0.1 and 0.5 C)and an impressively capacity(606 mAh·g^(−1)after 500 cycles at 1 C).The coordinated strategy of host–separator modifier is supposed to have wide applications in LSBs.

关键词： li_(2)ZnTi_(3)O_(8) lithium-sulfur batteries host-separator modifier catalytic conversion diffusion and loss of liPSs

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Aggregation of CeAlO_(3) inclusions in heavy ingot of a steel containing 0.007% aluminum

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Journal of Iron and Steel Research International 2024年第8期31卷 1960-1972页

作者： Qiu-yue Zhou Jun-tao Ba Lun Zhang Wei Chen ying Ren li-feng Zhang School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004HebeiChina School of Mechanical and Materials Engineering North China University of TechnologyBeijing 100144China

The distribution of inclusions at the bottom of a Ce-treated heavy steel ingot was detected and *** three-dimensional morphology and spatial distribution of CeAlOs clusters were characterized using the electrolytic extraction and Micro-CT detection.A model of inclusion collision to predict the aggregation of CeAlO_(3) inclusions in the ingot was established and validated by measured *** were mainly CeAlO_(3) and a small amount of Ce_(2)O_(2)S in the tundish after cerium *** collision and aggregation of inclusions led to the formation of large clusters in the ingot during the solidification *** slag entrainment inclusions,large CeAO_(3) clusters and small CeAlO_(3) particles were observed from the center to the edge of the ingot *** inclusions were mainly concentrated at the *** number density of inclusions larger than 200μm was 0.21 mm^(-3).The maximum diameter of CeA1O_(3) clusters was 1340μ*** the edge to the radial center and from the bottom to the top,the average diameter of inclusions gradually increased due to the longer solidification time of the ingot.

关键词： Inclusion Heavy ingot Cerium treatment Collision

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Advances in targeted therapy for human epidermal growth factor receptor 2 positive in advanced gastric cancer

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2318-2334页

作者： Ya-Kun Jiang Wei li ying-Yang Qiu Meng yue Department of Gastroenterology Central Hospital Affiliated to Shandong First Medical UniversityJinan 250013Shandong ProvinceChina Health Management Center Central Hospital Affiliated to Shandong First Medical UniversityJinan 250013Shandong ProvinceChina Yong Loo lin School of Medicine National University of SingaporeSingapore 119077Singapore

Emerging therapeutic methods represented by targeted therapy are effective supplements to traditional first-line chemoradiotherapy *** epidermal growth factor receptor 2(HER2)is one of the most important targets in targeted therapy for gastric *** combined with chemotherapy has been used as the first-line treatment for advanced gastric *** safety and efficacy of pertuzumab and margetuximab in the treatment of gastric cancer have been ***,monoclonal antibodies,due to their large molecular weight,inability to penetrate the blood-brain barrier,and drug resistance,lead to decreased therapeutic efficacy,so it is necessary to explore the efficacy of other HER2-targeting therapies in gastric ***-molecule tyrosine kinase inhibitors,such as lapatinib and pyrrotinib,have the advantages of small molecular weight,penetrating the blood-brain barrier and high oral bioavailability,and are expected to become the drugs of choice for perioperative treatment and neoadjuvant therapy of gastric cancer after validation by large-scale clinical trials in the ***-drug conjugate,such as T-DM1 and T-DXd,can overcome the resistance of monoclonal antibodies despite their different mechanisms of tumor killing,and are a supplement for the treatment of patients who have failed the treatment of monoclonal antibodies such as ***,after more detailed stratification of gastric cancer patients,various gastric cancer drugs targeting HER2 are expected to play a more significant role.

关键词： Human epidermal growth factor receptor 2 Gastric cancer Targeted therapy Review

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lithium-induced graphene layer containing li_(3)P alloy phase to achieve ultra-stable electrode interface for lithium metal anode

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Rare Metals 2024年第2期43卷 562-574页

作者： Jia-Xin Chen Guo-Qiang Zhang Xian-ying Qin Kui lin Zi-Jin Yang Ge-Meng liang yue Xia Guo-Bin Zhang Hai-Kun Wu Qiu-Chan Cai Hai lin Bao-Hua li Shenzhen Key Laboratory on Power Battery Safety Research and Shenzhen Geim Graphene Center Tsinghua Shenzhen International Graduate SchoolShenzhen518055China Shenzhen Graphene Innovation Center Co. Ltd.Shenzhen518107China School of Chemical Engineering and Advanced Materials The University of AdelaideAdelaideSA5005Australia Department of Chemistry City University of Hong KongHong Kong990077China

Uncontrolled growth of lithium dendrite will lead to low Coulombic efficiency and poor cycle stability,which hinders the commercialization of lithium metal ***,a novel modified lithium anode with reduced graphene oxide conductive network containing trace lithiophilic phosphorus(P-rGO/Cu)is prepared by electrospraying technique combined with heat treatment *** rGO layer has a concave and undulating conductive structure,which can significantly improve the effective electrical contact between lithium metal and the current collector,speed up the kinetics of interfacial electron transport and reaction,and improve the resistance of the negative electrode to the internal stress caused by volume change of the lithium,which is advantageous for the stability of the SEI *** extremely small and uniformly distributed red phosphorus element avoids the volume change caused by lithiation to the maximum *** two-phase compound li_(3)P obtained by alloying P with li can directionally induce the homogeneous nucleation and dense deposition of lithium metal,address the issue of lithium dendrites and extend the cycle life of the *** obtained P-rGO/Cu exhibits excellent electrochemical performance with an average Coulombic efficiency(CE)of 98%at a current density of 1 mA·cm^(−2) for 400 cycles,and the capacity retention rate of the full cell matched with lithium iron phosphate(LFP)is 83%after 400 cycles at 1C.

关键词： lithium anode Dendrite-free lithiophilicity Induced nucleation deposition

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Mechanical properties and microstructure of Mg-treated and Catreated industrial H13 steel after quenching and tempering

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Journal of Iron and Steel Research International 2024年第8期31卷 1987-2001页

作者： Jun-ying yue Dong Hou De-yong Wang Tian-peng Qu Xiang-long li Jun Tian School of Iron and Steel Soochow UniversitySuzhou 215021JiangsuChina

The effect of magnesium treatment and calcium treatment on the microstructure and mechanical properties of industrial H13 steel after quenching and tempering was *** impact toughness and tensile tests were mainly carried out,and the microstructure was observed by scanning electron microscopy,electron backscattered diffraction,and X-ray *** results show that magnesium treatment is still feasible in industrial *** is mainly manifested in the refinement of lath martensite and *** with calcium treatment,the prior austenite grains and carbides size of industrial H13 steel treated with magnesium decreased by 3.17μm after *** quenching and tempering,the carbides(especially V-rich carbides)in Mg treatment obviously spheroidized and distributed uniformly and increased in quantity *** lath martensite size is reduced from 2.45 to 2.31μ*** suggests that magnesium treatment was able to yield smaller grains and more evenly distributed ***,the impact toughness,yield strength,and ultimate tensile strength of industrial H13 steel with magnesium treatment increased by 28%,65.5 MPa and 123.7 MPa,*** increment of strength mainly comes from dislocation strengthening,grain refinement strengthening,and precipitation strengthening,among which precipitation strengthening accounts for the largest proportion.

关键词： Industrial H13 steel Mg treatment Heat treatment Carbide-Mechanical property

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