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A Cell-based High-throughput Screening Assay for Farnesoid X Receptor Agonists

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Biomedical and Environmental Sciences 2007年第6期20卷 465-469页

作者： ZHI-HUI ZHENG Guo-PING LV SHU-YI SI yue-SHENG dong BAO-HUA ZHAO HUA ZHANG JIAN-GONG HE Insitute of Medicinal Biotechnology Peking Union Medical College / Chinese Academy of Medical Sciences Beijing 100050 China College of Life Science Hebei Normal University Shijiazhuang 050016 Hebei China New Drug Research ＆ Development Center of North China Pharmaceutical Group Corporation National Microbial Medicine Engineering ＆ Research Center Shijiazhuang 050015 Hebei China

Objective To develop a high-throughput screening assay for Farnesoid X receptor （FXR） agonists based on mammalian one-hybrid system （a chimera receptor gene system） for the purpose of identifying new lead compounds for dyslipidaemia drug from the chemical library. Methods cDNA encoding the human FXR ligand binding domain （LBD） was amplified by RT-PCR from a human liver total mRNA and fused to the DNA binding domain （DBD） of yeast GAL4 of pBIND to construct a GAL4-FXR （LBD） chimera expression plasmid. Five copies of the GAL4 DNA binding site were synthesized and inserted into upstream of the SV40 promoter of pGL3-promoter vector to construct a reporter plasmid pG5-SV40 Luc. The assay was developed by transient co-transfection with pG5-SV40 Luc reporter plasmid and pBIND-FXR-LBD （189-472） chimera expression plasmid. Results After optimization, CDCA, a FXR natural agonist, could induce expression of the luciferase gene in a dose-dependent manner, and had a signal/noise ratio of 10 and Z＇ factor value of 0.65, Conclusion A stable and sensitive cell-based high-throughput screening model can be used in high-throughput screening for FXR agonists from the synthetic and natural compound library.

关键词： Farnesoid X receptor Agonist High-throughput screening Chimera

Correlation of pressure gradient in three hepatic veins with portal pressure gradient

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World Journal of Clinical Cases 2022年第14期10卷 4460-4469页

作者： Hao-Yu Wang Qing-Kun Song Zhen-dong yue Lei Wang Zhen-Hua Fan Yi-Fan Wu Cheng-Bin dong Yu Zhang Ming-Ming Meng Ke Zhang Li Jiang Hui-Guo Ding yue-Ning Zhang Yong-Ping Yang Fu-Quan Liu Department of Oncology The 9^(th)Clinical Medical College&Beijing Shijitan HospitalPeking UniversityBeijing 100038China Division of Clinical Epidemiology and Evidence-Based Medicine Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Interventional Therapy Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Gastroenterology Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of General Surgery Beijing Ditan HospitalCapital Medical UniversityBeijing 100102China Department of Gastroenterology and Hepatology Beijing You'an HospitalCapital Medical UniversityBeijing 100069China Department of Therapeutic Research for Liver Cancer The Fifth Medical Center of Chinese PLA General HospitalBeijing 100039China

BACKGROUND The liver is one of the most important organs in the human body,with functions such as detoxification,digestion,and blood *** terms of vascular anatomy,the liver is divided into the left and the right liver by the main portal vein,and there are three hepatic efferent veins(right,middle,and left)and two portal *** with impaired liver function have increased intrahepatic vascular resistance and splanchnic vasodilation,which may lead to an increase in the portal pressure gradient(PPG)and cause portal hypertension(PHT).In order to measure the increased pressure gradient of portal vein,the hepatic venous pressure gradient(HVPG)can be measured to reflect it in clinical *** accuracy of PPG measurements is directly related to patient *** To analyze the correlation between HVPG of three hepatic veins and PPG in patients with *** From January 2017 to December 2019,102 patients with PHT who met the inclusion criteria were evaluated during the transjugular intrahepatic portosystemic shunt procedure and *** The mean HVPG of the middle hepatic vein was 17.47±10.25 mmHg,and the mean HVPG of the right and left hepatic veins was 16.34±7.60 and 16.52±8.15 mmHg,*** average PPG was 26.03±9.24 *** correlation coefficient and coefficient of determination of the right hepatic vein,middle hepatic vein,and left hepatic vein were 0.15 and 0.02(P=0.164);0.25 and 0.05(P=0.013);and 0.14 and 0.02(P=0.013),*** mean wedged hepatic vein/venous pressure(WHVP)of the middle and left hepatic veins was similar at 29.71±12.48 and 29.1±10.91 mmHg,respectively,and the mean WHVP of the right hepatic vein was slightly lower at 28.01±8.95 *** mean portal vein pressure was 34.11±8.56 *** correlation coefficient and coefficient of determination of the right hepatic vein,middle hepatic vein,and left hepatic vein were 0.26 and 0.07(P=0.009);0.38 and 0.15(P<0.001);and 0.26 and 0.07(P=0.008),respecti

关键词： Portal hypertension Portal pressure gradient Hepatic venous pressure gradient Free hepatic venous pressure

Influence of Horizontal Flow on Atrium in High-Rise Residential Building

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湖南大学学报（自然科学版） 2009年第S1期36卷 15-18页

作者： LI Nian-ping,LI Jing,HE dong-yue,ZHANG Min-hui,HOU Su-juan(College of Civil Engineering,Hunan University,Changsha,Hunan 410082,China) College of Civil Engineering Hunan University Changsha 410082 China

In this study a 10-storied residential building model was employed to clarify the ventilation characteristics of the atrium and the rooms,which were effected by the different forms of lateral *** experiment was conducted under the combined effect of wind force and thermal buoyancy,and the similarity requirements were *** results have shown that the different forms of lateral openings cause the different ventilation effect of the building,and also have some certain *** conclusions provide a theoretical foundation for how to use the ventilation of atrium better in high-rise residential building.

关键词： high-rise residential building atrium model experiment horizontal flow natural ventilation

Disturbance Observer based Nonlinear Robust Attitude Tracking Controller for a Hexarotor UAV

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Disturbance Observer based Nonlinear Robust Attitude Trackin...

Realizing Stable Carbonate Electrolytes in Li-O_(2)/CO_(2)Batteries

第37届中国控制会议

作者： Bo Zhao dong yue School of Automation Nanjing University of Post and Telecommunication

This paper presents a novel nonlinear robust control law for a hexarotor unmanned aerial vehicle（UAV） with disturbance uncertainties. To attenuate the influence of the external turbulences effectively, an observer is designed to obtain the estimations. It is proved that the proposed attitude tracking control law guarantees exponential tracking of the reference trajectory and ensures all the signals of the closed-loop system globally uniformly ultimately bounded. Detailed numerical simulation results are provided to validate the control performance and effectiveness of the control scheme.

关键词： hexarotor UAV disturbance observer robust control nonlinear control

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Chinese Journal of Chemistry 2023年第3期41卷 314-321页

作者： Kai Chen Jia-Yi Du Jin Wang dong-yue Yang Jiang-Wei Chu Hao Chen Hao-Ran Zhang Gang Huang Xin-Bo Zhang State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchunJilin130022 China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefeiAnhui230026 China

The increasing demand for high-energy storage systems has propelled the development of Li-air batteries and Li-O_(2)/CO_(2)batteries to elucidate the mechanism and extend battery ***,the high charge voltage of Li2CO3 accelerates the decomposition of traditional sulfone and ether electrolytes,thus adopting high-voltage electrolytes in Li-O_(2)/CO_(2)batteries is vital to achieve a stable battery ***,we adopt a commercial carbonate electrolyte to prove its excellent suitability in Li-O_(2)/CO_(2)*** generated superoxide can be captured by CO_(2)to form less aggressive intermediates,stabilizing the carbonate electrolyte without reactive oxygen species induced *** addition,this electrolyte permits the Li metal plating/stripping with a significantly improved reversibility,enabling the possibility of using ultra-thin Li *** from the good rechargeability of Li2CO3,less cathode passivation,and stabilized Li anode in carbonate electrolyte,the Li-O_(2)/CO_(2)battery demonstrates a long cycling lifetime of 167 cycles at 0.1 mA·cm^(-2)and 0.25 mAh·cm^(-2).This work paves a new avenue for optimizing carbonate-based electrolytes for Li-O_(2)and Li-O_(2)/CO_(2)batteries.

关键词： Energy conversion Li-O_(2)/CO_(2)batteries Carbonate electrolytes Carbon dioxide fixation Oxygen heterocycles

Safety and efficacy of transfemoral intrahepatic portosystemic shunt for portal hypertension: A single-center retrospective study

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World Journal of Clinical Cases 2019年第12期7卷 1410-1420页

作者： Yu Zhang Fu-Quan Liu Zhen-dong yue Hong-Wei Zhao Lei Wang Zhen-Hua Fan Fu-Liang He Department of Interventional Therapy Peking University Ninth School of Clinical MedicineBeijing Shijitan Hospital and Capital Medical University

BACKGROUND Transfemoral intrahepatic portosystemic shunt (TFIPS) can be performed to treat portal hypertension. However, few studies have evaluated the safety and efficacy of this technique. AIM To retrospectively evaluate the safety and clinical outcomes of TFIPS and compare them with those of typical transjugular intrahepatic portosystemic shunt (TIPS). METHODS This retrospective study was approved by our hospital ethics committee. From November 2012 to November 2015, 19 patients who underwent successful TFIPS placement were included. In addition, 21 patients treated with TIPS during the same period were selected as controls. Data collected included the success rate and complications of TIPS and TFIPS. Continuous data were expressed as the mean ± SD and were compared using the Student’s t test. All categorical data were expressed as count (percentage) and were compared using the χ2 test or Fisher’s exact test. The Kaplan–Meier method was used to calculate cumulative survival rate and survival curves. RESULTS Baseline characteristics were comparable between the two groups. The success rate of TFIPS and TIPS was 95%(19/20) and 100%(21/21), respectively. Effective portal decompression and free antegrade shunt flow was completed in all patients. The portal pressure gradient prior to TIPS and TFIPS placement was 23.91 ± 4.64 mmHg and 22.61 ± 5.39 mmHg, respectively, and it was significantly decreased to 10.85 ± 3.33 mmHg and 10.84 ± 3.33 mmHg after stent placement, respectively. Time–to-event calculated rates of shunt patency at one and two years in the TFIPS and TIPS groups were not statistically different (94.7% vs 95.2% and 94.7% vs 90.5%, respectively). De nova hepatic encephalopathy was 27.5%(11/40) with five patients in the TFIPS group (26.3%) and six patients (28.6%) in the TIPS group experiencing it (P = 0.873). The cumulative survival rates were similar between the two groups: 94.7% and 94.7% at 1 and 2 years, respectively, in the TFIPS group vs 100% and 95.2%

关键词： Transjugular intraheptic portosystemic shunt Transfemoral intraheptic portosystemic shunt Portal hypertension Variceal bleeding

Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production

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Chinese Medical Journal 2011年第21期124卷 3499-3503页

作者： BAO Xu-dong yue Lin GAO Xue-jun Department of Cariology and Endodontology Peking University School and Hospital of Stomatology Beijing 100081 China

Background Streptococcus （S.） oligofermentans is a newly identified bacteria with a yet to be defined mechanism of sucrose metabolism that results in acid production. This study aimed to investigate the biochemical mechanisms of S. oligoferm-entans glucose metaolism. Methods The S. oligofermentans LMG21532, Lactobacillus （L.） fermentum 38 and the S. mutans UA140 were used to characterize sucrose metabolism by measuring lactate dehydrogenase （LDH） activity and lactic acid production. Continuous dynamics and high performance capillary electrophoresis were used to determine LDH activity and lactic acid production, respectively, from bacteria collected at 0, 10 and 30 minutes after cultured in 10% sucrose. Results These analyses demonstrated that LDH activity of the three bacterial strains examined remained stable but significantly different throughout the sucrose fermentation process. The S. oligofermentans LDH activity （（0.61±0.05） U/mg） was significantly lower than that of L. fermentum （（52.91±8.97） U/mg）. In addition, the S. oligofermentans total lactate production （（0.048±0.021） mmol/L） was also significantly lower than that of L. fermentum （（0.958±0.201） mmol/L）. Although the S. oligofermentans LDH production was almost double of that produced by S. mutans （（0.32±0.07） U/mg）, lactic acid production was approximately one sixth that of S. mutans （（0.296±0.058） mmol/L）. Additional tests examining pyruvic acid production （the LDH substrate） demonstrated that lactic acid concentrations correlated with pyruvic acid production. That is, pyruvic acid production by S. oligofermentans was undetectable following sucrose incubation, however, （0.074±0.024） and （0.175±0.098） mmol/L pyruvic acid were produced by S. mutans and L. fermentum, respectively. Conclusion S. oligofermentans is incapable of fermenting carbohydrates to produce enough pyruvic acid, which results in reduced lactic acid production.

关键词： Streptococcus oligofermentans lactate dehydrogenase pyruvic acid lactic acid

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网络环境下不确定时滞系统鲁棒H_∞控制

自动化学报 2007年第10期33卷 1093-1096页

作者：彭晨岳东南京师范大学电气与自动化工程学院南京210042

研究了网络环境下不确定时滞系统的鲁棒H_∞控制器的设计问题.在综合考虑网络的非理想状况下,建立一种新的网络控制模型.然后利用Lyapunov Krsasovskii泛函方法,通过引入自由矩阵,得到网络控制系统的H_∞鲁棒稳定条件和相应的鲁棒H_∞... 详细信息

研究了网络环境下不确定时滞系统的鲁棒H_∞控制器的设计问题.在综合考虑网络的非理想状况下,建立一种新的网络控制模型.然后利用Lyapunov Krsasovskii泛函方法,通过引入自由矩阵,得到网络控制系统的H_∞鲁棒稳定条件和相应的鲁棒H_∞时滞状态反馈控制器的设计方法.实例表明了本文所得结果的有效性.

关键词：网络控制系统 H∞控制最大允许综合时滞界线性矩阵不等式

Measurement of ^(79)Se by accelerator mass spectrometry using projectile X-ray technique

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Nuclear Science and Techniques 2000年第1期11卷 1-5页

作者： HE Ming JIANG Shan JIANG Song-Sheng WU Shao-Yong Zhao Zhan-Xia dong yue-An(China institute of Atomic Energy, Beijing 102413) 中国原子能科学院北京 102413

In order to carry out the measurement of heavy nuclei in accelerator mass spectrometry, characteristic X-rays of the incident projectile have been explored and used as a method for isobar discrimination. The projectile X-ray combined with AMS technique has been set up in China institute of Atomic Energy. The measurement of 79Se was performed by resorting to the projectile X-ray AMS technique and the detection sensitivity of 79Se was improved more than 2 orders of magnitude. The detection limit was about 3.6x 10-9 for 79Se/Se.

关键词： X射线技术硒79 重核原子核测量 AMS 加速器质谱