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Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage

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World Journal of Gastrointestinal Oncology 2024年第12期16卷 4716-4727页

作者： Yu-Bin Guo yue-ming wu Zhi-Zhao Lin Guangdong Provincial Key Laboratory of Gastroenterology Department of GastroenterologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina

BACKGROUND Colorectal cancer(CRC),the third most common cancer worldwide,has increasingly detrimental effects on human *** resistance diminishes treatment *** suggest that spermine synthase(SMS)may serve as a potential target to enhance the *** To investigate the association between SMS and radiosensitivity in CRC cells,along with a detailed elucidation of the underlying *** Western blot was adopted to assess SMS expression in normal colonic epithelial cells and CRC cell ***116 cells were transfected with control/SMS-specific shRNA or control/pcDNA3.1-SMS *** included cell viability,colony formation,and apoptosis via MTT assays,colony formation assays,and flow *** was studied in SMS-specific shRNA-transfected HCT116 cells post-4 Gy radiation,evaluating cell viability,colony formation,apoptosis,DNA damage(comet assays),autophagy(immunofluorescence),and mammalian target of rapamycin(mTOR)pathway protein expression(western blot).RESULTS Significant up-regulation of SMS expression levels was observed in the CRC cell *** down-regulation of SMS expression,cellular viability and colonyforming ability were markedly suppressed,concomitant with a notable increase in apoptotic ***,attenuation of SMS expression significantly augmented the sensitivity of HCT116 cells to radiation therapy,evidenced by a pronounced elevation in levels of cellular DNA damage and *** tantly,down-regulation of SMS corresponded with a marked reduction in the expression levels of proteins associated with the mTOR signaling *** Knocking down SMS attenuates the mTOR signaling pathway,thereby promoting cellular autophagy and DNA damage to enhance the radiosensitivity of CRC cells.

关键词： Spermine synthase Colorectal cancer Radiosensitivity Autophagy DNA damage

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Emerging roles of NRBF2/PI3KC3 axis in maintaining homeostasis of brain and guts

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Neural Regeneration Research 2022年第2期17卷 323-324页

作者： ming-yue wu Cui-Zan Cai Chuanbin Yang Zhenyu yue Ye Chen ZhaoXiang Bian Min Li Jia-Hong Lu State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao Special Administrative RegionChina School of Chinese Medicine Hong Kong Baptist UniversityHong Kong Special Administrative RegionChina Department of Neurology and Neuroscience Friedman Brain InstituteIcahn School of Medicine at Mount SinaiNew YorkNYUSA Department of Gastroenterology State Key Laboratory of Organ Failure ResearchGuangdong Provincial Key Laboratory of GastroenterologyNanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

NRBF2 has been identified as the fifth component of PI3KC3 complex and is required for maintaining the kinase activity to promote ***,the physiological and pathological roles of NRBF2are largely unknown.

关键词： PI3K homeostasis NRB

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Diversity analysis of soil dematiaceous hyphomycetes from the Yellow River source area:Ⅰ

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 829-834页

作者： Hao-qin PAN Jin-feng YU yue-ming wu Tian-yu ZHANG Hong-feng WANG Mycological Laboratory Department of PlantPathology Shandong Agricultural University Tai＇an 271018 China

Twenty-four soil samples of eight ecosystem-types around the Yellow River source area were investigated for the number and specific composition of soil dematiaceous hyphomycetes by dilution plate technique. And then the co-relationship between genus species of soil dematiaceous hyphomycetes and ecosystem-types was analyzed. The results show that the amount and species distribution of soil dematiaceous hyphomycetes had an obvious variability in different ecosystem-types, and that the dominant genus species varied in the eight ecosystem-types studied, with Cladosporium being the dominant genus in seven of the eight ecosystem-types except wetland. The index of species diversity varied in different ecosystem-types. The niche breadth analysis showed that Cladosporium had the highest niche breadth and distributed in all ecosystem-types, while the genera with a narrow niche breadth distributed only in a few ecosystem-types. The results of niche overlap index analysis indicated that Stachybotrys and Torula, Doratomyces and Scolecobasidium, Cladosporium and Chrysosporium had a higher niche overlap, whereas Arthrinium and Gliomastix, Phialophora and Doratomyces, Oidiodendron and Ulocladium had no niche overlap.

关键词： Yellow River source area Ecosystems Fungal species diversity Soil dematiaceous hyphomycetes Niche breadth Niche overlap

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Pharmacological insights into autophagy modulation in autoimmune diseases

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Acta Pharmaceutica Sinica B 2021年第11期11卷 3364-3378页

作者： ming-yue wu Er-Jin Wang Du Feng Min Li Richard D.Ye Jia-Hong Lu State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 9999078China Key Laboratory of Protein Modification and Degradation State Key Laboratory of Respiratory DiseaseCollege of Basic Medical ScienceGuangzhou Medical UniversityGuangzhou 510000China School of Pharmaceutical Sciences National and Local United Engineering Lab of Druggability and New Drugs EvaluationGuangdong Provincial Key Laboratory of New Drug Design and EvaluationSun Yat-sen UniversityGuangzhou 510000China Kobilka Institute of Innovative Drug Discovery School of Life and Health Sciencesthe Chinese University of Hong KongShenzhen 518000China

As a cellular bulk degradation and survival mechanism,autophagy is implicated in diverse biological ***-wide association studies have revealed the link between autophagy gene polymorphisms and susceptibility of autoimmune diseases including systemic lupus erythematosus(SLE)and inflammatory bowel disease(IBD),indicating that autophagy dysregulation may be involved in the development of autoimmune diseases.A series of autophagy modulators have displayed protective effects on autoimmune disease models,highlighting the emerging role of autophagy modulators in treating autoimmune *** review explores the roles of autophagy in the autoimmune diseases,with emphasis on four major autoimmune diseases[SLE,rheumatoid arthritis(RA),IBD,and experimental autoimmune encephalomyelitis(EAE)].More importantly,the therapeutic potentials of small molecular autophagy modulators(including autophagy inducers and inhibitors)on autoimmune diseases are comprehensively analyzed.

关键词： Autophagy Autoimmune disease Systemic lupus erythematosus Inflammatory bowel disease Rheumatoid arthritis Experimental autoimmune encephalomyelitis Autophagy inducer Autophagy inhibitor

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Antimicrobial Properties and Application of Polysaccharides and Their Derivatives

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Chinese Journal of Polymer Science 2021年第2期39卷 133-146,I0004页

作者： Gui-Xue Xia yue-ming wu Yu-Fang Bi Kang Chen Wei-Wei Zhang Shi-Qi Liu Wen-Jing Zhang Run-Hui Liu State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237China Frontiers Science Center for Materiobiology and Dynamic Chemistry Key Laboratory of Specially Functional Polymeric Materials and Related Technology(ECUST)Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

With the quick emergence of antibiotic resistance and multi-drug resistant microbes,more and more attention has been paid to the development of new antimicrobial agents that have potential to take the ***,as one of the major classes of biopolymers,were explored for their antimicrobial properties and applications,owing to their easy accessibility,biocompatibility and easy *** and their derivatives have variable demonstrations and applications as antimicrobial agents and antimicrobial biomaterials.A variety of polysaccharides,such as chitosan,dextran,hyaluronic acid,cellulose,other plant/animal-derived polysaccharides and their derivatives have been explored for antimicrobial *** expect that this review can summarize the important progress of this field and inspire new concepts,which will contribute to the development of novel antimicrobial agents in combating antibiotic resistance and drug-resistant antimicrobial infections.

关键词： Antimicrobial Drug resistance Polysaccharides Polysaccharide derivatives Chitosan

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Effects of phytoestrogen genistein on myocardial ischemia/reperfusion injury and apoptosis in rabbits

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Acta Pharmacologica Sinica 2004年第3期25卷 52-58页

作者： En-sheng JI,Hua yue,Yu-ming wu,Rui-rong HE1 Department of Physiology,Institute ofBasic Medicine,Hebei MedicalUniversity,Shijiazhuang 050017,China Department of Physiology Institute of Basic Medicine Hebei Medical University

AIM: To study the effect of genistein (GST) on rabbit heart ischemia/reperfusion (I/R) injury. METHODS: Rabbit heart I/R injury was induced by occluding the left anterior descending coronary artery for 45 min and reperfusing for 180 min. GST (1.0 mg/kg) was intravenously injected 5 min before heart ischemia. Hemodynamic data, infarct size, and cardiomyocytic apoptosis were measured. The pathologic changes of I/R myocardium were observed. RESULTS: During the I/R, heart rate, mean arterial blood pressure, myocardial oxygen consumption, left ventricu- lar (LV) –dp/dtmax and +dp/dtmax were decreased progressively. The infarct size was occupied 60.23 %±3.97 % (% of area at risk) in vehicle +I/R group while GST reduced the infarct size to 39.62 %±4.30 % (P<0.01). DNA ladder patter in myocardium was revealed by agarose gel electrophoresis in vehicle +I/R group while was not found in GST+I/R group. Apoptotic cardiomyocytes were sparse within ischemic myocardium at risk in GST+I/R group as compared with that in vehicle +I/R group (TUNEL stain). Apoptosis rate in ischemic myocardium from vehicle +I/R and GST+I/R groups detected by flow cytometry were 15.33 %±1.31 % and 3.88 %±0.33 %, respectively. Fas and Bax protein expressions in ischemic myocardium of vehicle +I/R group were higher than that in GST+I/R group (P<0.01). Bcl-2/Bax ratio in vehicle +I/R group was lower than that in nonischemic myocar- dium (P<0.01), while in GST+I/R group, the Bcl-2/Bax ratio was higher than that in vehicle +I/R group (P<0.01). CONCLUSION: GST reduced infarct size and apoptosis of myocytes in I/R rabbit heart.

关键词： genistein reperfusion injury apoptosis rabbits

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Facile Synthesis of High Molecular Weight Polypeptides via Fast and Moisture Insensitive Polymerization of a-Amino Acid N-Carboxyanhydrides

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Chinese Journal of Polymer Science 2020年第10期38卷 1131-1140,I0007页

作者： yue ming wu Wei-Wei Zhang Rui-Yi Zhou Qi Chen Chen-Yu Xie Heng-Xue Xiang Bin Sun Mei-Fang Zhu Run-Hui Liu State KeyLaboratory of Bioreactor Enginering East China Uniersity of Science and TechnologyShanghai 200237China State Key Laboratory for Modification of Chemical Fibers and Polymer Materals College of Materias Science and EngineringDonghua UniversityShanghai 201620China Key Laboratory of Specally Functional Polymeric Materials and Related Technology(ECUST)Ministry of Education Key Laboratory for Ultrafine Materials of Ministry of EducationResearch Center for Biomedical Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

Polypeptides have been widely utilized in the fields of biomaterials and *** since N-carboxyanhydride(NCA)was reported by Hermann Leuchs in 1906,ring-opening polymerization of NCAS has been extensively used to prepare *** continuous innovations,it is still challenging to synthesize polypeptides in high molecular weight *** address this challenge,we developed KHMDS/NaHMDS initiated fast NCA polymerization that is also moisture tolerant,open-flask amenable and terminal *** NCA polymerization was able to proceed in most common solvents and meet the solubility requirement of variable NCA monomers and corresponding *** can initiate r benzy-L glutamate-N carboxyanhydride(BLG NCA)polymerization in a reaction rate 92 times faster than does hexylamine and 80 times faster than does *** NCA polymerization also demonstrated easy and fast synthesis of gram-scale long chain polypeptides in an open flask.

关键词： NCA polymerization Polypeptide Open vessel Fast polymerization High Mw

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Duodenum has the greatest potential to absorb soluble non-ammonia nitrogen in the nonmesenteric gastrointestinal tissues of dairy cows

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 503-510页

作者： Ying-ming XIE Qing-biao XU yue-ming wu Xin-bei HUANG Jian-xin LIU Institute of Dairy Science Mo E Key Laboratory of Molecular Animal NutritionCollege of Animal SciencesZhejiang University

In cattle, dietary protein is gradually degraded into peptide-bound amino acids（PBAAs）, free amino acids（FAAs）, and ultimately into ammonia by the rumen microbes. Both PBAA and FAA are milk protein precursors, and the rumen and small intestines are the main sites where such precursors are produced and absorbed. This work was designed to investigate the expression of the peptide transporter Pep T1 and the AA transporters ASCT2, y＋LAT1, and ATB0,＋, and the concentrations of PBAA, FAA, and soluble protein in the rumen, omasum, and duodenum of dairy cows. Tissues and digesta were collected from six healthy Chinese Holstein dairy cows immediately after the animals were slaughtered. The expression of transporters was analyzed by real-time quantitative polymerase chain reaction（PCR）. The FAA concentration was assessed using an amino acid（AA） analyzer, PBAA concentration by quantification of AA before and after acid-hydrolysis by 6 mol/L HCl, and soluble protein concentration by quantification of the bicinchoninic acid content. The results showed that the relative abundance of m RNA of the transporters and the soluble non-ammonia nitrogen（SNAN） concentration of each fraction were greater in the duodenum than in the rumen or omasum. These results indicate that the duodenum is the predominant location within the nonmesenteric digestive tract for producing milk protein precursors. In addition, PBAA was the largest component of SNAN in the digesta from the rumen, omasum, and duodenum. In conclusion, the duodenum has the greatest concentrations of SNAN and PBAA, and the greatest potential for absorption of SNAN in the form of PBAA in the nonmesenteric gastrointestinal tissues of dairy cows.

关键词： Soluble non-ammonia nitrogen Nonmesenteric gastrointestine Transporters Dairy cows

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Targeting macrophage autophagy for inflammation resolution and tissue repair in inflammatory bowel disease

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Burns & Trauma 2023年第1期11卷 245-257页

作者： Er-jin Wang ming-yue wu Zheng-yu Ren Ying Zheng Richard D.Ye Chris Soon Heng TAN Yitao Wang Jia-Hong Lu State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR999078China Center for Metabolic Liver Diseases and Center for Cholestatic Liver Diseases Department of GastroenterologyThe First Affiliated Hospital(Southwest Hospital)Third Military Medical University(Army Medical University)Chongqing400038China Kobilka Institute of Innovative Drug Discovery School of Life and Health SciencesThe Chinese University of Hong KongShenzhen518172China Department of Chemistry College of ScienceSouthern University of Science and TechnologyShenzhen518055China Guangdong-Hong Kong-Macao Joint Lab on Chinese Medicine and Immune Disease Research University of MacaoChina

Inflammatory bowel disease(IBD)is a chronic,non-specific,recurrent inflammatory disease,majorly affecting the gastrointestinal *** to its unclear pathogenesis,the current therapeutic strategy for IBD is focused on symptoms *** is a lysosome-mediated catabolic process for maintaining cellular ***-wide association studies and subsequent functional studies have highlighted the critical role of autophagy in IBD via a number of mechanisms,including modulating macrophage *** are the gatekeepers of intestinal immune homeostasis,especially involved in regulating inflammation remission and tissue ***,many autophagic proteins and IBD-related genes have been revealed to regulate macrophage function,suggesting that macrophage autophagy is a potentially important process implicated in IBD ***,we have summarized current understanding of macrophage autophagy function in pathogen and apoptotic cell clearance,inflammation remission and tissue repair regulation in IBD,and discuss how this knowledge can be used as a strategy for IBD treatment.

关键词： Autophagy Inflammatory bowel diseases Inflammation Tissue repair Efferocytosis Macrophage

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含钛CDS-1分子筛的水热合成与表征

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化学学报 2008年第1期66卷 141-144页

作者：吴蕾蕾刘月明吴鹏何鸣元华东师范大学化学系上海市绿色化学与化工过程绿色化重点实验室

以钛酸正丁酯(TBOT)为钛源、发烟硅胶为硅源、四甲基氢氧化铵(TMAOH)为模板剂,采用一步法直接水热合成了CDO(Cylindrically Double Saw-Edged Zeolite One)晶体结构的含钛分子筛Ti-CDS-1.用粉末X射线衍射、电子扫描、紫外可见漫反射光... 详细信息

以钛酸正丁酯(TBOT)为钛源、发烟硅胶为硅源、四甲基氢氧化铵(TMAOH)为模板剂,采用一步法直接水热合成了CDO(Cylindrically Double Saw-Edged Zeolite One)晶体结构的含钛分子筛Ti-CDS-1.用粉末X射线衍射、电子扫描、紫外可见漫反射光谱、傅立叶变换红外光谱等手段对所合成的样品进行了表征.结果表明,Ti-CDS-1维持原来全硅分子筛的片状晶体外形;原料中钛原子大部分嵌入到CDS-1分子筛骨架,少量钛在分子筛表面形成八面体非骨架钛物种,这部分骨架外钛可以用酸处理选择性去除.

关键词： Ti-CDS-1 层状分子筛水热合成表征

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