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Arabidopsis calcium-dependent protein kinase CPK28 is potentially involved in the response to osmotic stress

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Chinese Science Bulletin 2014年第11期59卷 1113-1122页

作者： Anli Gao Qingyang Wu Yu Zhang yuchen miao Chunpeng Song State Key Laboratory of Cotton Biology Institute of Plant Stress BiologyCollege of Life SciencesHenan University

As calcium sensors, plant calcium-dependent protein kinases(CDPKs) play important roles in plants' responses to various abiotic stresses. Here, we report the functional characterization of CPK28, a member of the CDPK family in Arabidopsis, in response to osmotic *** cpk28 mutant, a loss-of-function mutant, exhibited an NaCl- and mannitol-sensitive phenotype in green cotyledons, while CPK28-overexpressing plants displayed stronger tolerance to NaCl and mannitol stresses than wildtype plants. Reverse transcriptase-polymerase chain reaction and beta-glucuronidase staining assays showed that NaCl and mannitol stresses induced CPK28. CPK28-overexpressing lines accumulated significantly more proline relative to wild-type plants and mutant plants under NaCl and mannitol stresses. Transient expression of CPK28-GFP in mesophyll cell protoplasts, as well as stable transgenic lines expressing CPK28-GFP, showed that CPK28 was localized in the plasma membrane. Expression levels of known stress-responsive genes were not significantly altered in the null mutant and overexpression lines,suggesting that CPK28 possibly mediated the stress response via the regulation of target proteins rather than via regulation at the level of transcription. Meanwhile, CPK28could autophosphorylate. Taken together, these data demonstrated that CPK28, a potential positive regulator, is involved in the response to osmotic stress in Arabidopsis.

关键词：钙依赖蛋白激酶渗透胁迫拟南芥聚合酶链反应 CDPK 应激反应转录水平非生物胁迫

Generation of the genetic mutant population for the screening and characterization of the mutants in response to drought in maize

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Chinese Science Bulletin 2014年第8期59卷 766-775页

作者： Zhiyong Gao Hao Liu Hongliang Wang Ning Li Daojie Wang Yuwei Song yuchen miao Chunpeng Song State Key Laboratory of Cotton Biology Laboratory of Plant Stress Biology Department of Biology Henan University

The autonomous Mutator(Mu)transposon in maize(Zea mays L.)lines 115F,330I,and 715D were crossed with inbred lines B73,Mo17,97108,and H9-21,M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool and The M2plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the *** we statistically analyzed these candidate *** drought stress,we screened seedlings at 3-leaf stage by using the infrared thermography,and successfully got 108 droughtinsensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines,which temperature were significant different with *** candidate mutants were primarily analyzed by infrared thermography,chlorophyll fluorescence,leaf water losing,photosynthetic characteristics,content of soluble sugars,soluble protein,and proline *** selected mutants were cloned by MuTAIL–PCR *** we provide the better genetic materials for research on maize breeding for drought tolerance.

关键词：干旱胁迫玉米育种突变体基因突变红外热成像鉴定筛选群体

Osmotically stress-regulated the expression of glutathione peroxidase 3 in Arabidopsis

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Chinese Science Bulletin 2007年第1期52卷 127-130页

作者： miao yuchen GUO JingGong LIU ErTao LI Kun DAI Jie WANG PengCheng CHEN Jia SONG ChunPeng Key Laboratory of Plant Stress Biology College of Life Sciences Henan University Kaifeng 475001 China State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China

Gene expression of glutathione peroxidase 3 (ATGPX3) in response to osmotic stress was analyzed in Arabidopsis using ATGPX3 promoter-glucuronidase (GUS) transgenic plants. High levels of GUS ex- pression were detected under osmotic stress in ATGPX3 promoter-GUS transgenic plants. Compared with the wild type, the growth and development of ATGPX3 mutants (atgpx3-1) were more sensitive to mannitol. In addition, the expression of RD29A, ABI1, ABI2 and RbohD in atgpx3-1 was induced by ABA stress. These results suggest that ATGPX3 might be involved in the signal transduction of osmotic stress.

关键词：渗透性谷胱甘肽过氧化反应细胞学

SRO1 regulates heavy metal mercury stress response in Arabidopsis thaliana

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Chinese Science Bulletin 2014年第20期59卷 3134-3141页

作者： Xiaoli Zhang Xiaoliang Zhao Baozhu Li Jinchan Xia yuchen miao State Key Laboratory of Cotton Biology Laboratory of Plant Stress Biology Department of Biology Henan University School of Basic Medicine Henan College of Traditional Chinese Medicine

Heavy metals in the environment are harmful limiting factors for the normal growth and development of ***,we isolated and identified an Arabidopsis thaliana T-DNA insertion mutant,named sro1-1,which showed a hyper-sensitive response to *** SRO1protein contains a WWE domain that mediates proteinprotein *** HgCl2treatment,when compared with the wild-type plants,the growth of sro1-1 was repressed dramatically and the number of true leaves was reduced and *** electrolyte leakage rates showed that cell membrane integrity in sro1-1 was damaged more severely than in the wild ***(3,5-diaminobenzidine)staining and confocal microscopy showed that Hg2?stress induced more hydrogen peroxide accumulation in sro1-1 than in the wild *** qRT-PCR results indicated that the expression of some abiotic stress-induced genes,such as L-ascorbate peroxidase(APX1),was reduced under oxidative or Hg2?*** plants containing a GFP::SRO1 fusion protein showed that SRO1was localized in the nucleus of the ***1 was shown to be expressed in various tissues,and was most highly expressed in the vigorous *** results suggest thatSRO1 may play an important role in the stress response of *** to heavy metals.

关键词：应激反应氯化汞重金属拟南芥抗坏血酸过氧化物酶定量RT-PCR 转基因植物 T-DNA插入

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Strigolactones interact with other phytohormones to modulate plant root growth and development

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The Crop Journal 2022年第6期10卷 1517-1527页

作者： Huwei Sun Weiqiang Li David J.Burritt Hongtao Tian Heng Zhang Xiaohan Liang yuchen miao Mohammad Golam Mostofa Lam-Son Phan Tran Laboratory of Rice Biology in Henan Province Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450046HenanChina Jilin Da’an Agro-ecosystem National Observation Research Station Changchun Jingyuetan Remote Sensing Experiment StationKey Laboratory of Mollisols AgroecologyNortheast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102JilinChina State Key Laboratory of Cotton Biology Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityKaifeng 475001HenanChina d Department of Botany University of OtagoDunedin 9054New Zealand Institute of Genomics for Crop Abiotic Stress Tolerance Department of Plant and Soil ScienceTexas Tech UniversityLubbockTX 79409USA Institute of Research and Development Duy Tan University03 Quang TrungDa Nang 550000Vietnam

Strigolactones(SLs),which are biosynthesized mainly in roots,modulate various aspects of plant growth and ***,we review recent research on the role of SLs and their cross-regulation with auxin,cytokinin,and ethylene in the modulation of root growth and *** nutrientsufficient conditions,SLs regulate the elongation of primary roots and inhibit adventitious root formation in eudicot *** promote the elongation of seminal roots and increase the number of adventitious roots in grass plants in the short term,while inhibiting lateral root development in both grass and eudicot *** effects of SLs on the elongation of root hairs are variable and depend on plant species,growth conditions,and SL *** or phosphate deficiency induces the accumulation of endogenous SLs,modulates root growth and *** analyses indicate cross-regulation of SLs with auxin,cytokinin,and ethylene in regulation of root growth and *** discuss the implications of these studies and consider their potential for exploiting the components of SL signaling for the design of crop plants with more efficient soil-resource utilization.

关键词： Cross-regulation Development Phytohormones Roots Signaling Strigolactones

Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum

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Science Bulletin 2022年第3期67卷 315-327页

作者： Chen Dong Ge Qu Jinggong Guo Fang Wei Shuwen Gao Zhoutong Sun Lifeng Jin Xuwu Sun Jean-David Rochaix yuchen miao Ran Wang Zhengzhou Tobacco Research Institute Zhengzhou 450001China Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475001China College of Biological Engineering Henan University of TechnologyZhengzhou 450001China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China Departments of Molecular Biology and Plant Biology University of GenevaGeneva 1211Switzerland

Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and *** this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nicotiana *** the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of *** of this enzyme in the ntggpps1 mutant leads to decreased carotenoid *** the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence *** activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput colorimetric assay.d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis compared with wild-type ***,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to *** findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield *** results open the door for the structure-guided rational design of elite genes in higher plants。

关键词： Rational design Carotenoid Methylerythritol phosphate pathway(MEP) Geranylgeranyl diphosphate synthase(GGPPS) Nicotiana tabacum

Anchorene,a carotenoid-derived growth regulator,modulates auxin homeostasis by suppressing GH3-mediated auxin conjugation

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Journal of Integrative Plant Biology 2024年第11期66卷 2490-2504页

作者： Danping Ke Yinpeng Xie Haipeng Li Liqun Hu Yi He Chao Guo Yahui Zhai Jinggong Guo Kun Li Zongyan Chu Junli Zhang Xuebin Zhang Salim Al-Babili Kai Jiang yuchen miao Kun-Peng Jia National Key Laboratory of Cotton Bio-breeding and Integrated Utilization State Key Laboratory of Crop Stress Adaptation and ImprovementHenan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityKaifeng 450046China Sanya Institute of Henan University Sanya 572025China State Key Laboratory for Crop Stress Resistance and High-Eﬁciency Production/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100China Institute of Plant and Food Science Department of BiologySchool of Life SciencesSouthern University of Science and Technology(SUSTech)Shenzhen 518055China The BioActives Lab Center for Desert AgricultureKing Abdullah University of Science and TechnologyThuwal 23955-6900Kingdom of Saudi Arabia Plant Science Program Biological and Environmental Science and Engineering(BESE)DivisionKing Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Kingdom of Saudi Arabia Laboratory of Cell Metabolism and Diseases State Key Laboratory of Conservation and Utilization of Bio-Resources in YunnanCenter for Life SciencesSchool of Life SciencesYunnan UniversityKunming 650500China

Anchorene,identified as an endogenous bioactive carotenoid-derived dialdehyde and diapocarotenoid,affects root development by modulating auxin ***,the precise interaction between anchorene and auxin,as well as the mechanisms by which anchorene modulates auxin levels,remain largely *** this study,we conducted a comparative analysis of anchorene's bioactivities alongside auxin and observed that anchorene induces multifaceted auxin-like *** genetic and pharmacological examinations,we revealed that anchorene's auxin-like activities depend on the indole-3-pyruvate-dependent auxin biosynthesis pathway,as well as the auxin inactivation pathway mediated by Group II Gretchen Hagen 3(GH3)proteins that mainly facilitate the conjugation of indole-3-acetic acid(IAA)to amino acids,leading to the formation of inactivated storage *** measurements indicated that anchorene treatment elevates IAA levels while reducing the quantities of inactivated IAA–amino acid conjugates and ox *** sequencing further revealed that anchorene triggers the expression of numerous auxin-responsive genes in a manner reliant on Group II ***,our in vitro enzymatic assays and biolayer interferometry(BLI)assay demonstrated anchorene's robust suppression of GH3.17-mediated IAA conjugation with ***,our findings highlight the significant role of carotenoid-derived metabolite anchorene in modulating auxin homeostasis,primarily through the repression of GH3-mediated IAA conjugation and inactivation pathways,offering novel insights into the regulatory mechanisms of plant bioactive apocarotenoids.

关键词： Anchorene auxin conjugation carotenoid-derived bioactive apocarotenoid Gretchen Hagen 3 root development

Local-contrastive-learning machine with both generalization and adversarial robustness: A statistical physics analysis

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 109-123页

作者： Mingshan Xie yuchen Wang Haiping Huang PMI Lab School of Physics Sun Yat-sen University Guangdong Provincial Key Laboratory of Magnetoelectric Physics and Devices Sun Yat-sen University

Distinct from human cognitive processing, deep neural networks trained by backpropagation can be easily fooled by adversarial examples. To design a semantically meaningful representation learning, we discard backpropagation, and instead, propose a local contrastive learning, where the representations for the inputs bearing the same label shrink(akin to boson) in hidden layers,while those of different labels repel(akin to fermion). This layer-wise learning is local in nature, being biologically plausible.A statistical mechanics analysis shows that the target fermion-pair-distance is a key parameter. Moreover, the application of this local contrastive learning to MNIST benchmark dataset demonstrates that the adversarial vulnerability of standard perceptron can be greatly mitigated by tuning the target distance, i.e., controlling the geometric separation of prototype manifolds.

关键词： machine learning statistical mechanics adversarial robustness neural networks

Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing

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Molecular Plant 2020年第8期13卷 1178-1193页

作者： Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix yuchen miao Xuwu Sun State Key Laboratory of Crop Stress Adaptation and Improvement State Key Laboratory of Cotton BiologyKey Laboratory of Plant Stress BiologySchool of Life SciencesHenan University85 Minglun StreetKaifeng 475001China College of Life Sciences Shanghai Normal UniversityGuilin Road 100Shanghai200234China Departments of Molecular Biology and Plant Biology University of GenevaGeneva1211Switzerland

The regulation of stomatal lineage cell development has been extensively ***,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been *** this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis *** identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker *** analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother *** of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these ***,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development.

关键词： molecular profiling stomatal development single-cell RNA-seq